18858261209 88208257 zhuow06@gmail.comB705

30312.13.148 0416.17.18 6064.6.12 7 611.13.19

(Endomembrane system)

endomembrane system

compartmentalization

Endoplasmic Reticulum, ER) 1945 Porter Claude

(laminal arrangement) vesicular arrangement) tubular arrangement)

A B A B

Cos-7

A B

1 5-6nm2

1:2 30Peptide Mass Fingerprint

-6-G-6-

P450

b5 GDP- NADH-b5

NADH-C -6- ATP - 5--

CKDLE HDLE4retention signal

1 Chaperonin 2 3 4

rough ER, rER

(smooth ER)

RERSER

How the growing polypeptide in ribosome is directed to the ER membrane?The signal hypothesis -- ER signal sequence

Structure of SRP (signal-recognition particle )

The signal-recognition particle (SRP) combining with ER signal sequence

ER signal sequences and SRP direct ribosomes to the ER membrane

How the growing polypeptide in ribosome passes through the ER membrane?

Structure of the Sec61 complex protein translocator

Peptide enter ER through a tunnel formed by large ribosomal subunit and translocator

What is the insertion mechanism of the ER transmembrane protein?

internal signal peptide N

A single-pass transmembrane protein with one internal signal peptide

The insertion of the multipass membrane protein rhodopsin into the ER membrane multiple internal signal peptide G

Signal peptide determines the destination of the synthesized protein

ER signal sequence retention signal ER retention signal ER retrieval signal nuclear import signal nuclear export signal peroxisomal targeting signalPTS transit peptide/ transit sequence

(glycosylation) N-N-linked glycosylation

Protein glycosylation in rough ER

1 2

Ca2+Ca2+

123

ER stress123

(Alzheimers disease, AD)

PS1PS1

(Golgi apparatus)1898Camillo golgi

3 ,.

. 60% 40%

continuous secretion discontinuous secretion

3H-10 3H-45

Warren Graham(Yale University) Jennifer Lippincott-Schwartz(National Institutes of Health) Fighting !!!The biogenesis of Golgi apparatusThe Golgi is totally from ER stop ER-Golgi transport the Golgi disappearsDuring mitosis: The GolgiERdaughter cells reappear from ERThe Golgi has its own remnantThe Golgi fragments in cytosol daughter cells reappear from aggregation

Lysosome 1955Christian de Duve 6nm60

primary lysosome secondary lysosome 1autophagolysosome 2heterophagic lysosome 3phagolysosome residual bodyAutophagy

1

2

3()()

1

(SiO2)

201317

2 40

Pompe-

-Gaucher110 Gaucher

330Allison

peroxisome 70Christian de Duve

The structure of peroxisome

40 1. RH2+O2 R+H2O2 . 2H2O2 2H2O +O2 3.

12

Thank you!

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