-250

-150

-50

50

150

250

350

450

550

650

750E

NA

-78

GR

O-

I-30

9IL

-8

MC

P-1

MC

P-2

MC

P-3

MD

C

MIP

-1

Ran

tes

SD

F-1

TA

RC

GM

-CS

F

MC

SF

Tpo

EG

F

IGF

-1

OS

M

PD

GF

VE

GF

SC

F

IFN

-y

IL-1

2

IL-1

5

IL-1

IL-1

b

IL-2

TN

F-

TN

F-

IL-3 IL

-10

IL-4

TG

F-

1

Ang

Lept

in

rela

tive

exp

ress

ion

expression FO/CO

expression WL/CO

chemokines CSF Growth Factors Immune stimulating cytokines

Immune suppressing cytokines

Others

Online supporting material; Supplemental figure 2

Supplemental Figure 2: Effects of moderate weight-loss (WL) or fish oil (FO) supplementation on cytokine expression profiles in

plasma of fasted moderately obese men as analyzed by a cytokine protein antibody array. Percent changes in expression at the

end of the fish oil (gray) and the weight-loss (black) period versus the control period (CO) are shown.