第九章 固有免疫的组成 细胞及固有免疫应答
DESCRIPTION
第九章 固有免疫的组成 细胞及固有免疫应答. 免疫分为 非特异性或天然免疫 ( Non-specific or innate immunity ) 特异性或获得性免疫 ( Specific or adaptive immunity ). The differences between the two types of immunity. 天然免疫. 获得性免疫. 抗原非依赖性( antigen-independent ). 抗原依赖性( antigen-dependent ). 立即( immediate )达到最大反应. - PowerPoint PPT PresentationTRANSCRIPT
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Non-specific or innate immunity Specific or adaptive immunity antigen-independent immediate antigen-dependent lag time patternMacromoleculesThe differences between the two types of immunity
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NKNKTB1T1 (neutrophils)2 (macrophages)
TB
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NK
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monocyte (macrophage, M)
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(
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A
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.pattern recognition receptor,PRR)pathogen associated molecular pattern, PAMP):
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scavenger receptorSRToll
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CLCLCLCLCLCLCLCLF2 NH2 CRDCOOH mannose receptorMR180 kDa8
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C SSCOOH COOHHOOCNH2NH2 2HNS scavenger receptorSR(scavenger receptor)
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TollLBP ()sCD14Soluble CD14LPS
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2.complement receptor, CRFcR
IgGC3b
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CD16 FcR IIICD11b/CD18 CD25CD28 CD32 Fc R II CD35 CR1 CD64 Fc R ICD71B7-2IL-2
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Adherence of bacteria via receptors
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A --- H2O2O2-NO B --- C ---
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pattern
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NADPH H+e- + O2O2-H+H2O2b558
II (NADPH) b558
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O2 + NADPH O2- + NADP + H+
2O2 + 2H2 H2O2 + O2
H2O2 + Cl + H+ HOCl + H2O
HOCl + R-NH RNCl + H2O
H2O2 + OCl- 1 O2 + H2O + Cl-
H2O2 + O2- O2 + OH- + .OH NADPH
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NO
NO
L- + 1/2O2 + NO
NO + O2 - ONOO-
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Oxygen-independent mechanisms of intracellular killingCationic ( )
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Macrophage Phagocytosis
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ADCC
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Th1
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--- (IL-1IL-6IL-8IL-12TNF-)-IL-1/12TNF--TGF-
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resting state
primed state-- IFN-
activated state-- C3b+LPS+IFN-
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CR1CR3 CR4 FclgG FcFcR// IgA Fc LFA-1IL-8R
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A. B. IgG-Ag FcR IgM CR
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Dentritic Cells, DC) nave) TDCT CD1aCD11cCD83 MLRT
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DC
PBMC1% DCDC DCLC DC DC DC
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DC DC
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TIR T
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DC a. b.FcR c. d. e.B7 f. DEC205
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DC a.MHC -III b.DC-CK T c. d.
T
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T BIgB
:
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NK(natural killer cell)(large granular lymphocyte, LGL TB-- TCRIg TCR-mIg-CD56+CD16+ --- NKCTLTh IFN510 12 NK
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Human NK cell
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NNK
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NK 1. 2.T --- A.NK --- CD3+NK --- CD3- B.NKT a. --- CD16 CD16+CD4-CD8- T CD16+CD4-CD8- NK b. --- NKCD56 CD3-CD4-CD8-CD56+ NK CD56- T 3. CD34+CD7+NKCD2+CD16+CD56+
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NKHLA 1.KIR IgKIR2DKIR3D ITIMKIR2DLKIR3DLKIR2DSKIR3DSITAMDAP12NK
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2.KLRCD94/NKG2ACD94/NKG2C3.KIRKLR
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NKCR MHC-I+NKNKMHC-I-KIRABMHC-IKAR NK(KIR)MHC I MHC I NK
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NK 1. 1 A./ B.Fas/FasL C.TNF-TNFR-
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2ADCC FcRNK+ IgG( )
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2. IFN-TNF-GM-CSFIL-3M-CSF A.TBAPC B.
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Antibody Dependent Cell-Medicated CytotoxicityADCCADCCIgGVFcNKFcRIgG
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NKCR NKCR IgNKCR KIR H2N NH2 -S-S- HOOC COOHITIMITIM COOHNH2ITIM: immunoreceptor tyrosine-based inhibitory motifs
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NK CD2CD16FcRIIICD69ADCC (antibody-dependent cell-mediated cytotoxicity)Target cell
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NK cells and their activation
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Electron micrograph of an NK cell
High power view of an NK cell granule containing perforin, granzymes, and other mediators
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Phase contrast micrograph of NK cell attacking a target cell projection.
An "NK" cell (N) attached to a "target" cell "T".. The NK cell will kill the now helpless target cell quickly, by the injection of deadly perforin
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NKT NK1.1TCR-CD3TCD4CD8TCD4TTCRTCRTCR CD1MHC
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1---FasL 2---NK1.1+T + DC + M a.DC+MIL-12NK1.1+TIFN-+IL-12Th0Th1 b.NK1.1+TIL-4Th0Th2 IgEIgG1
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. 1.FcR 2.IL-5CCchemokines, MCP-3
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A.,major basic protein B. C.IL-3IL-5 1. 2.
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(mast cell)(basophils) ---0.2% --- , 1.FcRIgEFc 2.C3aR C5aR
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1.IgE-Ag FcR 2.C3aC5a
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A. histamineC4leukotriene C4 D2prostaglandine D2 B. TNF IL-8PAF
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A. B.IgE C.
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1.2.3.-
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NKT NKTB1
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1.2.3.
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0-4h >96h APC4-96h NK
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0-4hM
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4-96hIL-8IL-6IL-1MCP-1(-1)LTB4(B4)PAF()PGE(E)-IFNTNF,IL-12
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96hAPC antigen-presenting cell
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1.pattern recognition receptor PRRpathogen associated patternPAMP2.Toll
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inflammation (acute inflammation)(chronic inflammation) -1 -
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PEICAM
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5- C3a C5aPGD2 LTC4LTD4TXEPAFN- PAFC5aLTB4PAF IL-8
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NKADCC
IgEFcIgE
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(ADCC)NKIgGFc(CDl6FcrR)IgGFcNKFcrRNKIgGNKADCC
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1. To1124(TLR2TLR4)G-G+TLR2G+TLR4G+
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2. ---