* 10-2009-0039413 []

* , , , 1 10,000 200,000 (dalton) (molecular weight cut-off) (ultrafiltration membrane) , , , . 10-2009-0039413 [] , !

*

! 5- 5- !

*(Saussurea lappa) (), (),

(Lycium chinense) (), (), ,

: encyber.com

*(Prunella vulgaris var.lilacina) (), (), ,

(Kochia scoparia) 1 (), ()

: encyber.com : herb.daegu.go.kr/

* 42~56 (C57BL/6, ) , (spontaneous alopecia)(melanocyte)

* 1.

(DWP-5000, ) 4 0.2 . 2.

(SM30-CE, ) 4 0.2 .

, , ( : 1,3- = 7:3) 400ml

* 1. , , ( : 1,3- = 7:3) 400ml (SM30-CE, ) 4 .

* 1. 1,2,3 , : 40% , : 1,2,3 1 1 100uL 28

* 2. 1,2,3 , 70% 1 DMEM 3 1 10% FBS DMEM 20% FBS DMEM 2 0.25% , 0.02% EDTA 2~3 10% FBS DMEM 2 X 104/mL 60 mm 5mL 37 24 FBS FBS 24 , (Thymidine) 10uCi 24 3 5% (trichloroacetic acid) 1 0.1% , 0.5N (scintillation cocktail) -(-counter) (uptake)

*(%) (%)

* 3. 1,2,3 V79-4( , ) MTT (Mossman T. J. Immunol. Meth. 55-63(1983)) . SLS

IC50 (%, w/v)SLS0.005 11.00 25.00 110.00

* 4. [ : Kimber (1990) : Identification of contact allergens using the murine local lymph node assay, J. Appl. Toxicol. 10(3); 173-180]. 20.0 %, 40.00 % 60.00 % . (Balb/c) 50 3 (auricular lymph node) [3H]-([3H]-methyl thymidine) 24 , - (Beckman LS 6000 TA, USA) (dpm, disintegrations per minute) . 60 % .

* . .

(107/mL)Cmp()2.132481 1.8715300.6 1.8823370.9 1.8723360.9

**