2002.8

immune(Antigen)

18

1618 16171818JennerVaccine()Vaccination

1920 Pasteur 1880 1882

1890 Metchnikoff1883

1897Behring(1890)1891Erlich 1897

701963Wright,Deuglas

20 20

64

Immune system

bone marrow B 40% thymus T bursa of fabricius B

lymphnodeB25%T75%(M)(DC)

(spleen) T25% B65% (M)(DC)

membrane immune systemMIS IgA TBMDC

Harder gland TB

(lyphocyte) TBNKT 1. ()

2.(1)T(T cell recepter,TCR)1015TcT

TCR

TCR TCR V 50 57D 2J 70 13VJ,VDJ 5070=3 103 57 2 13=1103 + +N V-J V-D,D-J ~1015

(2)(Erythrocyte receptor,ER)

CD2T ET B

TE

3PHAConA

PHAConAT T TB T

TPHA

4T TCluster of differentiationCD

CD2 TcERCD3 5-6TcTCR/CD3 CD4 THTHTCR HIVCD8 TcTcTCR

3. T

CD TC CD3+ CD4- CD8+ IFN-

TS CD3+ CD4- CD8+ TSFTB m TDTHTH1 CD3+ CD4+ CD8- MIFIFN- TH2 CD3+ CD4+ CD8- IL-4IL-5 B

B

1

B

21Surface membrane immunoglobulingSmIg B SmIgM B SmIgM+SmIgD 107BBSmIgMSmIgDB105SmIg SmIgTCRB

(2) FcBFc

3C3b B C3b

BEEEEEEEABEEEEEEEACFcRFcC3bRC3b

2. B B1 B2 B1CD5+ B2CD5- Sm Ig Sm IgM Sm IgMSm IgDIgG Fc +C3b + TI Ag TD Ag IgM IgMIgGIgAIgEIgD +

((natural kill cell,NKC) 1. FcCD16Fc KIR KAR2. ADCC

- Kupffer A :

() Fc C3b Ia_ M Ia+ M

1. 2.monocyte Ia M

a2 C1 C2 C3 C4 C5 PDI H IL-1IL-6IL-8TNF E

O2

O2n-

H2O2OH-IO2

3.Ia+ MThTCR

4.

MTNF

M

T -M -(DC) -Lc DCMHC-

.1. IgG FcADCCIgE Fc2. IgE Fc3. C3b -() 1982sigel()

4TCRBCRSmIgM SmIgDCD cluster of differtiation,CD TCD2CD3THCD4TcCD8CD CD

MHCMHCMHC HLA

DP DQ DR B C A D HLA- HLA- HLA- 1000~1200kb 1000kb 1600~2000kb 3500~4000kb

MHCHLA

1MHC-

(1) (2) MHC-I TcTCR

2 MHC-

TH immuno- assodiated antigenIa

3MHC-C2C4BfTNF ()(ImmunoglobulingIg)BIg (SmIgM SmIgD) B(IgM IgG IgA IgE IgD)

1.Ig

2Ig

3Ig

()complement system

1 20C1C1qC1rC1s------C911

256OC60OC30min

3. C1 C3

C1 C3IgG1IgM IgGIgA Ca++ Mg++ C1C1 C3PAD Mg++ C3 C2+C4 C4b2a C3(Bb) C3PA C3b +C3a C5 C5b + C5a C 56789 8-17

C3aC5a

()

1.

2(1) interleukin,IL IL-1~IL-23 ,(2) (colony stimulating factor, CSF) G-CSF M-CSF EPO T

1interferonIFN

IFN- IFN- IFN- T :

Antigen + ()+ +

10000dal10dal AA5734dal AA

1.2. 3.4. 5. 6. 7. 8.

antigenic determinants,AD BAD TAD: () (): AA,, : 56AA,, 918AA: BCRSmIg APC MHC-/-TCR (Th) MHC-/ -TCR(Tc)

- - - - - ,() - ( ,) Th -TD Ag -TI Ag

- FHN, (Ab) VP1VP4 - (Ab )T - ADT MHC- - (super Ag) ADT ( M)

(O)Ag 170 60,50 , AF (H)Ag 60 60,(a.b.c) , (1.2.3) Ag - , - (KAg) (Vi Ag) , 100,K88K99 Ag -(,,,0.3-0.4% ) -(,,)

(1.4.5.12i.1.2) OAg 1.4.5.12 HAg i,1.2

() () () ()

(adjuvant):10: : AE: (): +: IL-2IL-12IL-18DNA

()(Freund,s incomplete adjuvantFIA)Ag++80 AgAg ()(Freund,s complete adjuvant FCA) : FIA+: FIA MTB

(immune response)

TDAgTIAg

TDAg

(T cell mediated immunity,TMI)

Ag pro-Tc Tc

Ag Th Ag Th1(TDTC)

Tc -MHC-TCR - - Th1 ThAg50()

-() - -()

(B cell mediated immunity,BMI) TDAg APC Th Th2

Bc (IgMIgG) (IgG) TDAg

TIAg Bc (IgM)(Antibody,AbImmunoglubulin,Ig)() 1(primary response)(3-72-3) IgM

BIg

2 () () AbAbIgG

clone selection theoryAb33

AbCH IgMIgGIgAIgE IgD2. Ag Ab2 Ab3. Ag AbAb AbAb Ab AbAb

4AbIgGAbIgGIgMAbIgGIgMAb IgGIgMIgAAbIgGIgMIgGIgGIgM

5AbAbAbmonoclone Ab,McAbMcAbBB

McAb

() MHC-() Ab

VitAVitCFeZnSn

B Ag T Ag Ab2-3Ag 3-5Ag

. -Ab2 Ab

-

M

IL-1IL-6TNF- (1) (2) C3

(3) TNFIgGIL-8 (4) IL-12 Th Th1 NK()

1()C3()LPSMTNF-IL-1IL-6IL-12

2 AbIgG

IgG

IgMIgGC1IgGSIgA

(SIgA) (IgG)

1M IFN IL-12 NK2IFN-MTc

IgGIgGIgMC1+

IFN-NKIFN-NK

-IgGIgGIgMIgG-TcFasLIFN-

IgE

allergy AgAgallergen:

1. AbTDTH 2. AbTDTH

1 IgE2 3 4

1. 2. 2030min 1. 2. 3.

1 IgMIgGMNK2 1 A B A

2 + Aa+ Aa- Aa+ Aa- ()

3. 4

Ab(IgGIgAIgM)(,)Ag+Ab (Ag-Ab)nAb Ag

AgAb +

1IgGIgAIgMAg 2. 3.

1 Authus2103Ag

Ag Th1(TDTH)Ag Th1 Ag 1Ag48-72h 2

1 - ()0.lml72h 0.lml15-18h

2 Th1 Th 24-96h () : (1) (2) :

3MHC- Th1 : (1) (2)

-TET-TT

ETCD2TCD2

TE

. + + + 1.TB2.T

PHA+TPHA =(+)/200100%

TPHA

3H3H-T PHA+T+3H-TdR PHA+T 3H-TdR 3H-TdR CPM 3H-TCPM SI=CPM/CPM

Ag+T+M T+ M M M M M MIMI=M/M100%

TMTTPHAConA+T MTT formazan 595nmOD

()1 2 3 4 5

1

2 PH 7.0 T 370C Ag+(Ab) Ag-Ab PH3 T600C

3Ag-Ab41 - - - -2-80C

2 0.85%NaCl 6%8% 340C560C 370C 30min

4PHPH6.08.0PH7.07.2PH6PHPHPH7.07.2PBS

0.85%NaCl + ()

1 Ascoli

2

3 Ag Ab

Ag

+ ()

1 2 ()

3+ +() ( )

()Ab

123 4

1 -NDV 911d 2448h

-NDV+ 911d

2 (FMDV) 47d FMDV+ 47d 3 FMDV (IB-RS-2) (CPU)

FMDV+ IB-RS-2 CPU

1.2.

1.F+ Ab AbF(FITC) AbF + Ag Ag- AbF Ag- AbFAbF

F F Ag Ag

AbF AbF

2.1 2

3. Ag+AbR Ag-AbR AgR+Ab AgR-Ab R

4 ()

--1.(1)2,()()

1234

2.+1%+123

3. ++(1) (2)4.()

:(+) -- ///// ::

1.+K88

2.1

+

2

()

3

4

5

3.

. , SRBC , ,

-5---

1 2

(/) 1 12 - 1 5 1 10 - 0.5ml 15 2 25 1 4550 1 ++ 0.51ml 1020 + ++ 0.51ml +

(/) 1 1 35 1 + 0.5ml 25 1 1 2 1520 2

(/) 25 2 15 30 1.5ml 10 2 20 2 4

(/) 30 2 45 1 O 1 50 1 1 70 3 O 1

(/) - 24 2- 4 2 2 2 O 2 () 2-4 2

1520 0.5ml/ 1) 0.5ml/ 2) 0.5ml/ (A) 2ml/ 1) 2) 1ml/ (A) 2ml/

1. 2.

1.2.