中華牙醫學會第三十二屆學術研討會...

中華牙醫學會第三十二屆學術研討會中華牙醫學會第三十二屆學術研討會

中華民國口腔顎顏面放射線學會九十八年度學術研討會中華民國口腔顎顏面放射線學會九十八年度學術研討會

27th November, 200927th November, 2009

陳玉昆，陳玉昆， Y-K ChenY-K ChenDepartment of Oral Pathology,

School of Dentistry, College of Dental Medicine,

Kaohsiung Medical University,Kaohsiung, TAIWAN

虛擬顯微鏡於口腔顎顏面病理學實驗課程之應用

An Application of Virtual Microscopy to theTeaching of Oral & Maxillofacial Pathology

Laboratory Course

參考資料1. Evans AJ, et al. Primary frozen section diagnosis by robotic

microscopy and virtual slide telepathology: the University Health Network experience. Human Pathology 2009;40:1070-81.

2. Dee FR. Virtual microscopy in pathology education Human Pathol 2009;40: 1112-21.

3. Weinstein RS, et al. Overview of telepathology, virtual microscopy, and whole slide imaging: prospects for the future. Human Pathol 2009;40:1057-69.

4. Kaohsiung Medical University, Department of Oral Pathology.5. Marchevsky AM, et al. Self-instructional “Virtual Pathology”

laboratories using web-based technology enhance medical school teaching of pathology. Human Pathol 2003;34:423-9.

6. Chen YK, et al. An application of virtual microscopy to the teaching of oral and maxillofacial pathology laboratory course. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2008;105:342-7.

7. Hanks CT, et al. Internet- and DVD-based instruction in oral pathology for dental students. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2004;98:195.

8. Farah CS, et al. The e-evolution of microscopy in dental education. J Dental Edu 2009;73:942-9.

9. Dotslide, Olympus company catalogue

Virtual microscopy: emulate traditional light microscopy using digital image files (virtual slides) manipulated on a computer screen using microscope emulator software

Refs. 4, 9

Whole slide imaging: create digital images of the entire area of a glass slide

Hard Copy

Soft Copy

顯微影像數位掃瞄系統

Automate scan process- 50 slides

Virtual microscopy: use computer mouse to perform the control functions ordinarily handled with a traditional light microscope (position the objective lens)

Ref. 4

Ref. 4

OlyVIA閱片

Dot slide掃瞄、閱片

Procedures of whole slide scanning (1)Dot slide

Ref. 4

New slide


Ref. 4

Overview image


Ref. 4


Ref. 4

Edit scan area


Ref. 4


New area

Ref. 4

Live (active)


Ref. 4

Live (active)


Autofocus (AF)

Ref. 4

Start


Ref. 4


Ref. 4

Live (active)


Ref. 4


Ref. 4

Save file

ExtendedFocal Imaging

(EFI)


Ref. 4


Extended Focal Imaging (EFI)

Virtual slide viewer: use iconic representations of 4×, 10×, 20×, and 40× objective lenses and select the magnification of choice by clicking on one of these icons with a computer mouse

Ref. 4

OlyVIA

Refs. 4, 9

Net Image Server/Web Server, Dotslide and OlyVIA

Dot slide/OlyVIA

Dot slide/OlyVIA

Lan/Internet

Dot slide/OlyVIA

Dot slide/OlyVIA

Net ImageServer

Dot slide Web Server

Workstation Clients

Telepathology: practice of pathology at a long distance, has advanced continuously since 1986

Robotic controlof microscopy

Ref. 1

Frozen Section Telepathology UHNToronto Ontario Canada

Virtual microscopy or telepathology - attractive to educators due to:(1) nearly perfectly simulates the pan & zoom characteristics of conventional microscopy(2) versatility of computer-assisted education

Refs. 3, 8

Medical schools in the US: entirely digital for pathology courses, discarding light microscopes, and building virtual slide laboratories

UCLA School of Medicine’sCenterRef. 5

UCLA School of Medicine’s Center

Ref. 5

Table 1. Mean Ratings of Attributes of VirtualPathology Based on a Likert Scale (1, Not at

All Useful to 5 Very Useful), n = 72

Attributes of the Virtual Pathology Module Mean

Standard Deviation

Quality of images 4.4 0.69

Image description 4.1 0.87

Case histories framing the slides 4.1 0.88

Pretest 3.5 1.05

Posttest 3.7 0.98

Feedback on posttest 3.2 1.33

Overall format of presentation 4 0.89

Ease of navigation 4.6 0.63

Overall gain in learning 4.0 0.81

Presence of experts to assist me 4.2 0.75

UCLA School of Medicine’s Center

Ref. 5

Table 2. Mean Ratings of Student Perceptions of VirtualPathology Based on a Likert Scale (1, Not at

All Useful to 5 Very Useful), n = 72

Criteria for Student Perceptions Mean

Standard Deviation

I enjoyed working on the web-based slide presentation 3.4 0.62

This format was an effective way to learn the given content 3.4 0.62

I was able to complete all of the sections within the time limit 3.7 0.46

The petest helped me in focusing on what I did not know 3.0 0.85

I prefer this interactive format to a lecture presentation 3.2 0.76

Oral Pathology Laboratory course for dental students at University of Michigan - converted from a microscope-based course to an internet-accessible virtual histopathology ‘‘laboratory.’’ (2003) Ref. 7

University of Queensland, School of Dentistry (Brisbane, Australia) implement VM technology (Aperio Systems’ ImageScope) into its courses of Oral Biology (2006) & Oral Pathology (2008) Ref. 8

口腔病理學 (含實驗 ) - KMUOP1. 屬於基礎範圍，為提昇同學學習之興趣，於 2006年開始應用網路及多媒體教學，架構口腔病理科教學網頁

2. 假設虛擬顯微鏡，將口腔病理教學玻璃切片轉換成高解析度的數位影像檔，置放於本科網站中。下載 OlyVA軟體，使用寬頻網路閱覽口病教學切片。

Refs. 4, 6

Ref. 4Ref. 4

Ref. 4

Remote log in

Ref. 4

……….

Log in

User name

Password

administrator

Ref. 4

Open remotefile

Ref. 4

Search now

Ref. 4

Ref. 4

Open

Ref. 4

Ref. 4

Refs. 4, 6

高醫牙科口腔病理學實驗切片線上教學

小組線上教學

發表文章： An application of virtual microscopy in the teaching of an oral and maxillofacial pathology laboratory course. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2008;105:342-7

線上考試

口病實驗期末考

( 請務必寫上姓名與學號 )

姓名學號

內容：1.寫出口腔病理診斷2.標示口腔病理特徵

( 考試時間為 50 分鐘 )

Ref. 4

注意事項• 點選 OlyVIA 後，工具列中點選 remote login ，

鍵入個人帳號、個人密碼及 IP Address:163.15.173.75 ， max.Hits: 改為 500 、下載考試題目

• 考試當天，於口病網頁：oralpathol.dlearn.kmu.edu.tw 下載考試用ppt 檔於 D 槽，務必更改檔名為自己的姓名與學號，如 ’陳大明 9512345’ 。 ( 完成下載考試用 ppt 檔於 D 槽後，請務必關閉口病網頁，否則視同作弊 )

• 作答完成後，檢查無誤後，請將個人 ppt 檔儲存至老師提供之 USB ，最後請登錄 USB之號碼於點名簿上，方可離開電腦教室

( 請務必詳細閱讀 )

Ref. 4

請寫下題目 1-8 之病理診斷

請以英文作答，錯字扣 1 分

題目病理診斷得分1 Squamous cell carcinoma (M to P-D)2 AAA3 BBB4 CCC5 DDD6 EEE7 FFF8 Nevus (intramucosal)

得分 (A)

Ref. 4

請標示下列病理特徵題目病理特徵得分

A aaa

B bbb

C Nevus cells ( 示範從 OlyVIA 下載圖片之方法與練習用 )

D ddd

E eee

F fff

G ggg

H Abnormal mitosis( 練習用 )

I iii

J jjj

得分 (B)

總得分 = A + B =Ref. 4

從 OlyVIA下載圖片之方法 (1)

點選 Copy Display to Clipboard ，複製適合的圖片

Ref. 4

從 OlyVIA下載圖片之方法 (2)

BackNevus cells

Ref. 4

C. 請從題目 1-8 中找出並標示 Nevus cells 之病理特徵從 OlyVIA下載圖片之方法 (3)

貼上所選擇之圖片後，插上標誌，如星號 ( 可插上多個星號 )

貼上所選擇之圖片後，插上標誌，如箭號

Ref. 4

H. 請從題目 1-8 中找出並標示 Abnormal mitosis (009) 之病理特徵( 請自我練習 )

< 注意 > 請勿複製新投影片 Ref. 4

作答完成• 請務必更改 ppt 檔名為自己的姓名與學號，

如 ’陳大明 9512345’• 再將此 ppt 檔儲存至老師提供之 USB• 最後請登錄 USB 之號碼於點名簿上，方可

離開電腦教室

Ref. 4

Advantages of virtual microscopy vs traditional microscopy

Ref. 2

Accessibility- Access can be anywhere anytime there is computer (and the Web) available- One slide can be viewed by many or duplicated and shared Multiple recuts are not needed- One-of-a-kind slides that cannot be recut can be duplicated into an unlimited number of copies and shared with others, e.g. fortuitous cut in following case


Refs. 1, 2

Efficiency- Focus, proper condenser adjustment, and lighting are not required-Technical competence in viewing is easier to achieve and less frustrating for trainees who do not have an aptitude for traditional microscopy- There is rapid access to the next slide in the slide box


Refs. 2, 4

Pedagogic- Very-low-power over (<<x4) allows to better visualize relationship of pathologic to normal tissue- A thumbnail & location box allows trainee to remain oriented to the whole slide while viewing a high magnification


Ref. 2

Pedagogic- Marking x-y & magnification coordinates of multiple key areas & movement among these areas at the click of the mouse is possible


Ref. 2

Pedagogic- Side-by-side viewing, annotation overlays, & integration with descriptions, case scenarios, gross & radiological images or digital photomicrographs is possible


Ref. 2

Pedagogic-Group discussion is enhanced as each computer screen can serve as a multihead microscope

Conference

Disadvantages of virtual microscopy vs traditional microscopy

Ref. 2

Pedagogic-Trainees do not learn how to use a traditional microscope if virtual microscopy is used exclusively

Technology- Low magnification has less resolution when viewed on a standard computer screen- Refractile objects do not refract well- Original glass slide tissue artifact and imperfections are difficult to scan- Virtual focus acquisition & viewing is inefficient, especially over the Web

Ref. 2

Venues for virtual microscopy implementation

Education in Pathology1. Medical student education - Pathology small group teaching - Histopathology laboratories/large groups - Integrated and problem-based curricula - Repositories for sharing among institutions2. Cytology3. Hematology4. House staff education and evaluation5. Continuing medical education6. Veterinary pathology and comparative pathology7. Histology

Although the production of virtual microscopic slides remains a time-consuming task and the cost of archiving the images digitally is still considerably higher than archiving on glass slides, virtual microscopy still has many advantages over traditional glass microscopy in oral and maxillofacial pathology education

Ref. 6

Microscopic virtual images may one day be incorporated into digital databases for clinical diagnosis, adding the histopathological images as part of medical digital charts of patients, as is currently the case for images through teleradiology

Ref. 6

In 2005, DICOM established a special working group (Working Group 26) to develop extensions to the standard for telepathology imaging and pathology in general

2 significant limitations on single image objects within DICOM(1) DICOM image objects pixel dimensions are stored as 16-bit integers, for a maximum value of 64K, inadequate for virtual slide telepathology(2) DICOM image objects data size are stored as 32-bit integers, for a maximum value of 2 GB. This may need to be adjusted upward for some virtual slide telepathology applications.

Ref. 3

감 사 합 니 다 謝謝

中華牙醫學會第三十二屆學術研討會...

Documents