1.antibody, Ab B 2.immunoglobulin, Ig (1) (2) membrane immunoglobulin, mIg B (secreted immunoglobulin, sIg) ,

()heavy chainH 2 light chainL 2 ()variable region, V constant region, C () hyper-variable region, HVR), (complementary determining region, CDR framework region, FR ()hinge region

domain :VLCL :VHCH1CH2CH3(IgGIgA IgD)CH4IgMIgE1.

2.(1)VHVL (2)CH13CL Ig(3)CH2(IgG)CH3(IgM) C1q (4)CH2CH3(IgG) (5)CH3(IgG) FcR(6)CH4(IgE) FcR

Immunoglobulin Fragments: Structure/Function Relationships

1.Papain IgG Fab(fragment of antigen- binding, ) Fcfragment crystalizable, 2.Pepsin IgG F( ab)2 pFc

3.Ig

1.joining chainJ) 2.secretory piece, SP

isotype IgC

1.H IgG (gamma) IgA (alpha) IgM (mu) IgD (delta) IgE (epsilon) (1) (2)IgGIgG1, IgG2, IgG3, IgG4 IgAIgA1, IgA2IgM: IgM1, IgM2

2.(C) (1) (kappa) (lambda)(2)() OZ(+) (1) 190() OZ(-) (2) 190() Kern(+)(3) 154() Kern(-)(4) 154()

allotype CHCL (genetic markers ) GmGm130 AmA2m1A2m2 KmKm1Km2Km3

idiotype BVTBV

1. H14 2.2 3.22

Ig 1. V(100) J(5) C(1) 2. V(30) J-C(4) 3.H V(95) D(27) J(6) C(9) V

Mouse antibody genes

Human antibody genes

Ig 1. BV-D-JCHclass switching 2. B IgM IgGIFN- IL-4IgEIL-5IgA

V

IgG, IgE) 2 (IgA) 4 (IgM) 105

Ag 1.2.

C 1. Ab(IgMIgG) + Ag C1q IgG4IgA IgE 2. (1)opsonizationIgG + () FcR (2)ADCCIgG + () Fc RNK (3) 3.

IgG 1. (1) (2) (3)(75%Ig) (4)(2123) (5)35(8.017 mg/ml) (6)SPA

2.(1)(2)(3)(4)ADCC3.(1)(2) (3)()(4) (5) IgG (6)

IgM 1.(900 kd) 2.Ig 3. Ig 4.Ig510% 5. 5 IgM 6. 7.IgM IgM 8.BIgM(mIgM) 9.

IgA 1.

2. 6 3.Ig515% 4. 5.IgA IgA1

IgA2,

Origin of Secretory Component of sIgA

IgD 1. 2. B, 3. 1B 2 4.Ig1% 5. 3

IgE 1. 2.(Ig0.002%) 3. 3 4. 5. 6. IgE

polyclonal antibody 1. B:2. 1 2

monoclonal antibody, McAb

1. B 2. 3. B 4. HAT(H), (A) (T)

5. 1 2 3

(genetic engineering antibody) 1. DNAIg 2. -(chimeric antibody) (reshaped humanized antibody) (bispecific antibody)

1.2.3. 4.