DNARNADNARNA

6 DNARNA 1 2DNA. 1DNAmRNA 2mRNA 1 2 3 3 1 2

1 DNA RNA

FOU?

DNApg

CML E.coli

1. 2. 3. 4.

DNAmRNAprotein

CT

reverse genetics 1.DNA2.DNADNA BANK3.DNA 4.genetic marker.5.()6.7.8.

1./// 1 2/ 3/ 4/PCR 5 6//2.1

1.Southern DNA2.Northern RNAmRNA 3. 4.

(PCR PCRPCRDNA single strand conformation polymorphism,sscpDNAPCRPCRSSCP

DNADNADNADNA DNADNA DNA

1 2 2 3 4 5 1mRNA 2mRNA 3mRNA 1 2 3

3 mRNA

1.DNADNADNA 2.DNA 1 PCR/ASO 1a.b./ 2DNAPCR DNAASO

3 / /2 SSCPPAGE DNADNASSCPPAGE

DNACDNA // /PCR PCR

5313425313

1RFLPPCRPCR/RFLP DNARFLPRFLPRFLP DNA10020CMRFLP

3 a b c aRFLPRFLP bRFLP cRFLPRFLP d

9/922/922/22kb-9/9kbkan-3BamHIRFLPabRFSP DNARFLPDNA5%1%RFLP

2CA PCR/PAGEP146

1mRNA12RTPCR2mRNA RTPCR/PCR3mRNA NorthernRTPCR PCR

1.Hb . Rbc

4.Hb Punjab Hb55.6%16P30Hb121GAAGlu) CAAGln EccRIDNAPCR 33DNA144bpEcoRI3C1PCRPCR/ASO40/104bpHbPuN Job 144bp,40/104bpDNAMnl IHbE 26 GluLys))C1C2PCRC3PCR/RFLPRNAHbmRNART/PCRRT-PCRmRNAPCRmRNAmRNA

DMDBMD

1. DMDBMD 2. XP21.2121.3DMD5201/3500 BMDDMD1/30000 3. 1DNA60%DMDBMD25%32545~55

4.DMDDNADMDBMDDNA

1 XP21P202cDNAcDNA3PCRPCR9DNA80%DMD4RFLPCADMD

5.DMDRNA

RTPCR2.4MBc14kb

PKU 1 21

2phe 313/4PKU 11PAHPAH 2PAH11399GTAvalGTTvalPKU PAH

4DNAPCR/ASOPKURFLPPCR/SSCPPKU5 399 RFLP PKU8 1PAHRFLPECORIECORI11/17kbPKURFLP 2PCR/ASODNAPCR/PAHASO399ASO DNA/ASO; ;a1PKU399ATPKU a. bPKLLECLRIRFLPc DNAASO

1/5000~1/50000

XXqFX0,1%186kb26PCR/ASORFLP

X1%FIXFIXFIX398FIXXq26q2734kb8

FIXcDNADNAFIX5RFLPFIX5kbDNA2~3+2+13ab

4FcDNAVIIIDNADX18St14PCR/BCLIXbaI RFLPPCR/SSCPFVIII18BCL IDNA

1HAV7500HAVcDNACDNARNASSRNARNAHBVHBVDNA3.3kbPCRPCREBHDVHDVSSRNA1678 DNAcDNARNART/PCRELISA21EcoliEcoli;(2) 34PCR3PCR

1.1DNA22. DNA 1 2

JkB DNA 1 2B DNA221 2J1 21 2kB

C-myc bc1-2 t14;18)( q32;qI1) 85% 12C-onc

1.229CML22phCML9095%phCML9229q+22q-

2.1.pht9;222.C-onc C-ab c-sis9223.CML DNAbcr9c-abl5100kb225.8kbbcr4.CMLc-abl/bcrmRNA5.bcr CMLbcrbcr Bcrph BcrCMLALLAML PCRbcr/ablCML

DNA DNASou-thernDNAsouthernDNA

1.JeffreysRFLP100bp 2.161732333F4623F6bp 3.DNAsouthernblotDNA 4.DNADNA

11976DNAABCMD2ABCD3ANorthern blot B Western blot C dot blot D. Southern blot4PCRABCD

5DNAABCD

6ABCD

7ADNABDNACDANDDNA

8A2050B2060C2070D2080

9SangerA23B24C34D35

102090ABCD

11ABCD

12APCR/ASO BPCR/SSCP/sequencing C RT/PCR D RFLP

13.- A6P13.3- Pter B17 P13.3-PterC18 P13.3- Pter D19 P13.3- Pter

14- A2 B3 C4 D5

15 A BC D

16B- A BC D

17DMDDMD ABCD

18 A B C PAHDCK

19A BC D

20SOUTHERNDNA A DNA BRNAC D

1 A BC D

2 A B C D

3 A BC D

4 A BPCR CDNA D

5 ADNA BPCRCDNAPCR DDNA

6ABmRNACD

7ADNARNABCD

8 A B C D

9 AcDNA B CDNA DRNA10 A B C P D35S1 2DNA3 RFLP1234 12 34

1A1976-2CX1

3.D southern blot4.D

1DNARNADNARNA2DNADNA3Mg+Mg+4RNADNA5PH7.27.2

637945572

5BPAGESSCPPAG6B DNADNAIIII7DDNADNA8C Sanger

9A 3`5`243410A 11C 12A PCR/ASODNAASOPCR/SSCP/SEQUENCINGRT/PCRRFLP

13A 14 B15C 16D 17C 18A19D 20A 1985JeffreysDNASouther blot DNARNA

1 AB 2 ABCD MDNA3 A B C D 4 ABC5 ABC6ABD A D

7AD 8ABCD 1 10-5~10-6 /10-7~10-8/2 3 49 ABCD10ABC

1 (gene diagnosis) 2 DNADNA finger--printing 1985 SouthernDNASouthernDNA

1 6123DNARNA4DNA5 DNAmRNA mRNA:a. 6

2 mRNA 3DNAPCRDNAPCR41985DNA DNA

1.

DNA RNA ,FOU, .,DNApg.

12CMI3

2

(reverse genetics)1DNA2 DNA3DNA4genetic mark;5678

1/// / / /PCR // 21 43