RNA

N

NMetSer ThrAlaValCysGlyPro N12 N

N

HspATP

:

:

()

(autoradiography) (pulse-chase experiment)(microsomes)

?

Porter1945endoplasmic reticulumER

rough endoplasmic reticulumrER smooth endoplasmic reticulumsER

rER

rER

rER

sER

1.

2.

3.

N-

4. Sec61p

BiPER

5. P450 6-

Golgi bodyGolgi apparatusGolgi complex1898Camillo Golgi

GB(forming face)(cis face) (mature face)(trans face)

Regions of the Golgi apparatus

cis transTPP NADP

1. (cis Golgi)(cis Golgi network, CGN) cis CGN

2.

3.TGN TGN TGNTGN TGN

: 40%, 60%; : 20%, 50-60%; : 60%, 40%;

1. GBGB

2. rERERGBERGBGB N Asn-NH2 O SerThrTyr-OH

3. rERGB GB N

4. GB GB

,50,,,

(heterogenous),0.250.8m1m,25-50nm

pH4.6-4.8Cl-

:50(hydrolytic enzymes)pH4.6

(primary lysosome)(secondary lysosome)(residual body) (post lysosome)(tertiary lysosome )

-6--6-

-6- (mannose 6-phosphate, M6P)M6P

N--6-N- (N-acetyglucosamine phosphotransferase) N-[)]

(signal patch) , ,

6-

M6P(M6P receptor protein)M6PpH6.57M6P(pH=6)M6PM6P

(peroxisome)(microbody)

1. P19873

2. A

H2O2 H2O2 H2O2

3.

1. 2.3 3.

4. 5. -6-

(protein targeting)(protein sorting)

signal sequence sorting receptor

15-60N

15-35N-1N-6-12C-

12(SRP)SRPA3 SRP(DP)

75SRP64 SRPDP SRPGTPSRP

( signal recognition particle, SRP)docking protein, DP( translocon)

signal recognition particle, SRP617SRNAG: SRP

SPR

Docking protein,DPSRPG:, SRP

Sec61 2nm

Sec61, Sec61 10( translocon)

Signal hypothesis

(start-transfer sequence)(stop transfer sequence)

N- N-N- (internal signal sequence) N- (noncleavable)

N

(stop transfer sequence)

NN

NN

NC+-

coat

? ? ? ?

(coated vesicles)(clathrin-coated vesicle)COPII(COPII coated vesicles)COPI(COPI coated vesicles)

1. COP ERGB

ER

COPII-

2. COP I 8ARFSar Lys-Asp-Glu-LeuKDEL SRPCLys-Lys-X-X COP I

ER

Cop I Vesicles

Cop I and II Vesicles

3. TGN

Clathrin coated vesicles

Selective transport by clathrincoated vesicles

dynamin1.5nmpinch off

SNAREs soluble NSF attachment protein receptor20SNAREsv-SNAREst-SNAREs v-t-SNAREsSNAREstrans-SNAREs complexes

SNAREs

NSF

RabsRabstargeting GTPaseGTP30RabsRabsRabsGDPGTPSNAREsRabseffector

Rabs in docking

(molecular chaperones)

2Bipheavy-chain binding protein)Bip1

BiPER

1.2.