细胞在生物材料表面的粘附及影响粘附的因素 李湖燕
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细胞在生物材料表面的粘附及影响粘附的因素 李湖燕. 细胞与材料的相互作用是组织工程领域研究的重点领域之一，细胞必须首先与材料粘附（adhere），才能进行下一步的迁移(migration)、分化(differentiation)和增殖(proliferation)。. 生物识别（biorecognition） 细胞膜表面的受体（receptor）——细胞外与其相对应的信号分子配体（ligand）. 细胞表面： 细胞膜外层的寡糖外被、 磷脂双份子层细胞膜、 表层溶胶. - PowerPoint PPT PresentationTRANSCRIPT
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adhere(migration)(differentiation)(proliferation)biorecognitionreceptorligand
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adhesion molecules
cadherins(integrins)16724
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ECM:collegenECMfibronectinECMproteoglycanlaminin
extracellular matrix,ECMECMligandRGD--
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focal adhesions,FAs
protein complexes(cytoskeleton)(ECM)anchoring the celltransmitting signals 10-15nm
(talin)(paxillin)(vinculin)tensinfocal adhesion kinase
actin
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tyrosine kinase,Src(focal adhesion kinase)InRNA
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migration
focal complex0.25(focal adhesion)
http://www.reading.ac.uk/cellmigration/migration.htm
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topography
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1.Exploring and engineering the cell surface interface .Molly M. Stevens Julian H. George2. Dalby, M. J., et al., Nat. Mater. (2007) 6, 997
- Hydrophilicity/HydrophobicityVoglerself-association
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Hydrophilicity/Hydrophobicity:();(RGD);Cellsurface interactions of rat tooth germ cells on various biomaterials.Journal of Biomedical Materials Research Part A.
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(fanctional group) ;;()
Effects of surface functional groups on protein adsorption and subsequent cell adhesion using self-assembled monolayersYusuke Arim, Hiroo Iwata.
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(surface charge) BaldwinNGFInteraction of cells on chargeable functional group gradient surfaces. Jin Ho Lee, Jin Whan Lee etc.