. 1. 2. Mendel,(phenotype),,(allele),(gene).

Morgan,(linkage),(gene map).. 1. :(chromosome),,,(nucleic acid).2.1928, Griffith.1944, Avery.transformation:DNA.

. DNA1. Chargaff

2. Blender test

3. DNA Watson & Crick1953DNA(double helix).P:, dR :G : , C : T :, A :

4. (central dogma)DNARNA

DNADNADeoxyribonucleic acid

Polynucleotide and its componentPurinePyrimidineadenineguaninecytosinethymineuracilriboseadeninedeoxyribosenucleotidenucleotidenucleoside

. Phosphodiester Bond,1 2 3 4

. DNADNADNARNA

. DNA

. DNA1.

anneal

2. Melting temperature (Tm)3. pH4. (hybridization)

. DNADNADNADNANICK

1. DNA: DNA2. DNA: DNA,,,(linking number, Lk). a. : ,. b. DNA,,,.

c. :;(ethidium bromide, EB).d. (topoisomerase) I: DNA,,1. II: ATP,DNA,DNA,2.,.

e. palindromeStemloop structure of DNA

f. (extinction coeffecient)mgml-1, 1 mgml-1ds DNAA26020,ss DNARNAA26025.:1. ()2.

RNA. 1. DNA.2. .3. nucleotide,.4. 90%r RNA

,RNAsplicing

. :,RNase.

. RNA

1. mRNAGenomic DNA,RNA.mRNA. mRNA,,RNA(hnRNA: heterogeneous nuclear RNA)

2. rRNA

S(Svedberg),,,.

3. tRNAStem structureLoop structureAmino acidanticodon

4. snRNA small nuclear RNA,RNAsplicing.mRNAguanine

5. RNARNA(ribozyme): rRNA,splicing,RNA.,RNA. Ribonuclease P

.

. 1.

2.

. 1. (primary structure)

2. (secondary structure)

3. (tertiary structure)

4. (quaternary structure)

. ,,.

. (domain):.2. ():,-.

. 1. : . :(gel filtration chromatography)SDS-PAGE

: (ion-exchange chromatography)(isoelectric focusing) : ,,,. (affinity): His-tag,

2. HLMGSHLVDALELVMGDRGFEYTPKAWLVV8T1: HLMGSHLVDALELVMGDRT2: GFEYTPKT3: AWLVV1: HLMGSHLVDALET2: LVMGDRGFET3: YTPKAWLVHLMGSHLVDALELVMGDRGFEYTPKAWLVEdman degradation

3. SDS-PAGEMALDI-TOF MS (Matrix Assisted Laser Desorption Ionization, Time Of Flight MASS )

4. X5. .6. (proteome) .

Questions

,.,.