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, 2008-6-912

DNA3///1: ATG AAC ACG 2: ATG AAC ACG 3: ATG AAC ACG 4: ATG AAC ACG ..

1: ATG AAC ACG 2: ATG AAT ACA 3: ATG AAC ACG 4: ATG AAC ACG ..

1: ATG AAT ACG 2: ATG AAC ACA 3: ATG AAC ACG 4: ATG AAC ACG ..

41: ATG AAG ACG 2: ATG AAC ACG 3: ATG AAC ACG 4: ATG AAC ACG ..

1: ATG AAG ACG 2: ATG AAG ACG 3: ATG AAG ACG 4: ATG AAG ACG ..

1: ATG TTC ACG CCT 2: ATG TTT ACG CCT 1: ATG TTC ACG CCT 2: ATG TTT TCG CCT TTCTTTPhedSACGTCGThrSerdNN5

6miRNA miRNA753miRNA21-24nt

1 miRNA/2miRNA/

3 857mtmtmtmtmtmt+p=80/(152+80)=0.727 = p/2t = 0.727/(2 70 106) = 5.210-9 (miRNA/)miRNATargetmiRNATargetTarget??miRNA/9

2.1 miR397a AmiR535 BAB10miRNATargetNew targetTargetgenemiRNAmiRNANew target /1113/miRNAmiRNA67miRNA12DatasetRegionsKsPKaPTotal5flanking1.2280.0931.387e-060.1890.0161.824e-04Binding site0.5730.082/0.1250.026/3flanking1.4240.0929.46e-080.1860.0171.259e-03 2.1 95miRNAmiRNA13miRNATarget genenRegionPositionLength (bp)S (10-3) (10-3)5' flanking1-19719755.756.46mir156Os3989029Binding site198-218210003' flanking219-79727962.022.935' flanking1-45545510.150.56mir159Os5966029Binding site456-476210003' flanking477-77830232.092.545' flanking1-21221222.532.45mir390Os1010027Binding site213-233210003' flanking234-68945621.041.145' flanking1-363612.387.65mir395Os0993024Binding site37-57210003' flanking58-79373641.661.475' flanking1-2727000mir408Os1534025Binding site28-49220003' flanking50-64659710.560.445' flanking1-4040000mir820Os0201024Binding site41-61210003' flanking62-61054921.790.985' flanking/161.21.51.802.85AverageBinding site/21.20003' flanking/536.53.01.531.582.2 6miRNAmiRNA14

2.2 miRNA(A)miRNA (B)AB1 5.210-9miRNA/2miRNA::target3)/miRNA4miRNA15 DNA 16

3.1 1112272TIGR51712GT CATGTTC ACGCCT AGGT CATGTTT ACGCCT AGGCVSVSVS VS 18

3.2 G+C19

3.5 G+C (GCRs = 0.019, P = 0.649; GCRs = 0.000, P = 0.993)20

3.3 (AT, AC, AG, TC, TG, CG)21

12322

3.4 23Intron endPosition (base pairs)Constraint51-20.977 (0.000)3-60.394 (0.001)7-100.128 (0.001)31-21.000 (0.000)3-60.351 (0.001)7-160.007 (0.001)3.2 ()24Sites Type of nucleotide changeACCGAGTCATTG4-fold sites0.0132(0.0015)0.0155(0.0017)0.0283(0.0024)0.0211(0.0021)0.0126(0.0017)0.0130(0.0017)FEI0.0187(0.0020)0.0193(0.0025)0.0384 (0.0029)0.0355(0.0025)0.0200(0.0018)0.0178(0.0018)First intron0.0092(0.0014)0.0127(0.0018)0.0196(0.0021)0.0233(0.0021)0.0098(0.0013)0.0094(0.0014)3.3 4 FEI(1) (2) (3) (4) 25 G+C26

4.1 G+CG+C >65% (G+C)27GGCCCGCAGCTCGGCGCGC28GCGC 20~59?29GenesIntron number per gene0123456>6TotalHgene 115825724211237321Lgene5561533631281940321Arabidopsis567549 3532181937321 4.1 321G+CG+C30

4.2G+C31

4.3 322GC4GC32

4.4 G+CCCGCCTCCTGCGCGCT->G33AACodonRCSUAACodonRCSUHgeneLgeneHgeneLgeneAlaGCA+0.1780.928AsnAAC*1.7591.084 GCC*1.8231.206 AAU+0.2410.916 GCG*1.7670.92ProCCA+0.3531.147 GCU+0.2310.946 CCC*1.0430.794CysUGC*1.8671.349 CCG*2.2641.005 UGU+0.1330.651 CCU+0.341.054AspGAC*1.7320.982GlnCAA+0.2360.742 GAU+0.2681.018 CAG*1.7641.258GluGAA+0.1840.691ArgAGA+0.2531.065 GAG*1.8161.309 AGG+1.391.673PheUUC*1.8751.271 CGA+0.2430.455 UUU+0.1250.729 CGC*2.1721.246GlyGGA+0.3040.816 CGG*1.6790.978 GGC*2.5171.418 CGU+0.2630.583 GGG0.930.894SerAGC*1.7741.296 GGU+0.2490.872 AGU+0.1320.708HisCAC*1.6861.037 UCA+0.2511.083 CAU+0.3140.963 UCC*1.821.165IleAUA+0.2080.61 UCG*1.7790.77 AUC*2.5191.382 UCU+0.2450.977 AUU+0.2731.008ThrACA+0.241.085AAA+0.1670.613 ACC*1.6981.26 AAG*1.8331.387 ACG*1.8480.74LeuCUA+0.1270.483 ACU+0.2140.915 CUC*4.1041.663ValGUA+0.0810.412 CUG*2.1451.469 GUC*1.7581.164 CUU+0.2591.081 GUG*1.9631.42 UUA+0.0370.345 GUU+0.1991.004 UUG+0.3270.958TyrUAC*1.8711.199 UAU+0.1290.8014.2 (*) G+CG+C (0.05)(+) G+CG+C34

4.5 (CAI)4.5 G+CG+C35tRNAGCDNA1miRNAmiRNA23GCG+C36 BIOINPLANT

37