結構生物學 期末報告

指導老師 鄒文雄 學生 林彥君

從蛋白結構看 arabidopsis tubby-like protein2 的 F-box domain 和ASK1 蛋白，形成 SCF complxe的可能性

Skp1&F-box in SCF

http://au.expasy.org/tools/blast/

>Tlp2Fbox

SPWASLPPELLHDIIWRVEESTAWPARAAVVSCASVCKSWRGITMEIVRIPEQCG

http://au.expasy.org/tools/blast/

>ASK1(arabidopsis)MASVGIAPNPGARDSTGVDKLPEEMNDMKIRDDKEMEATVVDGNGTETGHIIVTTIGGRNGQPKQTISYM

AERVVGHGSFGVVFQAKCLETGETVAIKKVLQDRRYKNRELQTMRLLDHPNVVSLKHCFFSTTEKDELYL

NLVLEYVPETVHRVIKHYNKLNQRMPLIYVKLYTYQIFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVK

LCDFGSAKVLVKGEPNISYICSRYYRAPELIFGATEYTTAIDVWSAGCVLAELLLGQPLFPGESGVDQLV

EIIKVLGTPTREEIKCMNPNYTEFKFPQIKAHPWHKIFHKRMPPEAVDLVSRLLQYSPNLRSAALDTLVH

PFFDELRDPNARLPNGRFLPPLFNFKPHELKGVPLEMVAKLVPEHARKQCPWLGL

No 1LDK

Cul1-Rbx1-Skp1-F Boxskp2 SCF Ubiquitin

Ligase Complex

PDB ID:1LDK Source: Homo sapiens Deposition Date: 08-Apr-2002 Classification: Ligase

SCF complex (1LDK)

模擬 ASK1 在 SCF complex 中的位置

模擬 ASK1 立體結構

模擬 F-box 在 SCF complex 中的位置

模擬 F-box 立體結構

ASK1&F-box in SCF complex

ASK1

F-box

Conclusion

Mutant arabidopsis tubby-like protein2 (P52L) of F-box resulted in reduction of the interacting activity with ASK1(C.L Lee, 2004)