異嗜性抗體阻滯試劑與試管 之問與答
DESCRIPTION
Heterophilic Blocking Reagent. F requently A sked Q uestions. 異嗜性抗體阻滯試劑與試管 之問與答. Q1. 什麼時候會需要 HBR?. 正在發展三明治免疫檢測。 曾有檢測到假陽性反應之檢體。 確認檢體為假陽性,而非真陽性檢體。 檢測結果受到 HAMA 或異嗜性抗體干擾。 查出影響檢測結果之假陽性檢體類別。 檢測需要阻滯劑。阻滯劑為 HAMA 阻滯劑或異嗜性阻滯試劑 ? 需要一個通用阻滯劑加至各種檢測系統。 評估一個阻滯劑的要素。. Q2. 何謂異嗜性抗體干擾 ?. - PowerPoint PPT PresentationTRANSCRIPT
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Heterophilic Blocking ReagentFrequently Asked Questions
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Q1. HBR?HAMAHAMA?
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/ (species) (epitode) (solid phase)125I 1 (labeled antibody)2 Q2. ? 1 2
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Q3. HBR ?IgG IgG) (binding) (affinity) K-value =105-106
HBR HBR (binder) HBR (steric hinderance) HBR K-value = 109 HBR
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HBR 1000 HBR
1. HBR2. HBR 3. HBR anti- (anti-rabbit, anti-goat, anti-sheep, anti- mouse) 4. HBR subtypes (anti-mouse IgG1, anti-mouse IgG2)5. HBRQ4. HBR ?
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IgG IgM 40 10 (documented) Fc (multi-component) EIA RIA Fc (conjugation) Q5. ?
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() () () (,,)Q6. ? (AIDS) (OKT3) (E. Coli)
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Q7. ?
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HAMA
Q8. HAMA ?HAMA (Human anti-mouse antibody, )
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()
(1) HAMA (human anti-mouse antibodies)(2) (HBR) (HBT)
Q9. (Blocking Reagent)?
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(1) HAMAHAMA IgG (passive blocking agents) IgG (double monoclonal)
(2) (HBR) (HBT) (active blocking reagents) (kinetics)Q9. (Blocking Reagent)?
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HBR :a. HAMA b. c. d. e. Scantibodies HBR Scantibodies HBR ( IgG Scantibodies HBR (spike) (recovery)Scantibodies HBR Scantibodies HBR (reproducibility) Scantibodies HBR Scantibodies HBRIgGScantibodies HBR (conjugates) Q10. HBR
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Scantibodies (donors)IgG
Q11.
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Q12. HBR
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