מעבדה 7 ביכימיה
DESCRIPTION
דוח מעבדה מספר 7 בקורס מעבדה בביוכימיה א' , אוניברסיטת בר אילןTRANSCRIPT
7: Ka ,
80-213-01
:7.12.14, ' 8:00-11:00
:
203184262
:1. Ka 2. Ka 3. , ,
'- (Ka) 25 c: PKa Ka
4.751.8*10-5CH3COOH ()
3.741.8*10-4HCOOH
9.404*10-10HCN
9.226*10-10H3BO3
7.216.2*10-8NaH2PO4
Ka - 54321 '
98421 0.1M (")
12689 0.1M (")
5.736.056.487.347.66pH
0.090.080.040.020.01 (M) [HX]
0.010.020.060.080.09 (M) []
0.954-0.602-0.1760.6020.954Log ([X-]/[HX])
: :
1:::
2::: 3:::
4::: 5:::
Pka Y PH 1 :PKa=6.6166 1 : , . Pka =6.6166 Pka= -
ka=
, PKa =7.21 Ka = ( 80) (NaH2PO4) PH
'- :98.587.576.565.554.543.532.521.510.50NaOH(ml)
12.0811.8511.3810.6210.129.959.739.59.248.918.043.222.722.512.32.091.911.721.7PH
0.00180.00170.00160.00150.00140.00130.00120.00110.0010.00090.00080.00070.00060.00050.00040.00030.00020.00010 Naoh (mole)
:76.565.554.543.532.521.510.50NaOH(ml)
12.3212.2612.1311.9511.3610.159.489.188.818.457.812.842.322.021.92PH
0.00140.00130.00120.00110.0010.00090.00080.00070.00060.00050.00040.00030.00020.00010NaOH(mole)
: 0.2 0.0001
? : = PH=2.25 = PH . .
( ' ) ? 3 4 " 4 " 0.2 4 " 0.012 0.2 0.012 0.0024 ( )
? , , ( ) . 0.2mol -> 1LX mol ->0.004LX=
:1L - > 0.2mol0.0065L -> XX= () 8x10-4 1.3 x10-3 () () : mol acid ->1.3 x10-3 mol baseXmol acid -> 1mol acid () , V=9ml=0.009LC=0.2Mn=0.0018mole
: ( )V=7ml=0.007LC=0.2Mn=0.2x0.007=0.0014mole
:1mol acid1.73X--->3.925= 0.0567mg/ml
5. ( ). 1 0.0567 " 10 0.567 " : 1 -> 10 -> 5 : : g/mol 536.87 : :
:. -, . : , 3 (