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    ��56� S���& Se6+�:� ��& -��G��# �-�G�5�

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    caspius(. -��.�� 'c5�5�I�5*� ( 'c5�5�.*��

    2)3 :(88-69.

    Almeida P.R. 2003. Feeding ecology

    of Liza ramada (Risso, 1810) (Pisces, Mugilidae) in a south-western estuary of Portugal. Estuarine, Coastal and Shelf Science, 57: 313–323.

    Ameida P.R. 2003. Feeding ecology of Liza ramada (Risso, 1810) (Pisces, Mugilidae) in a south- western estuary of Portugal. Estuarine, Coastal and Shelf Science, 57: 313–323.

    An L., Hu J., Yang M., Zheng B., Wei A., Shang J. and Zhaho X. 2011. CYP1A mRNA expression in redeye mullets (Liza haematocheila) from Bohai Bay. Marine Pollution Bulletin, 62(4): 718–725.

    Bain P.A., Hutchinson R.G., Marks A.B., Crans M.J. and Schuller K.A. 2013. Establishment of a continuous cell line from south bluefine tuna (Thunnus maccoyii). Aquaculture, 59(3): 59–63.

    Boglion C., Costa C., Giganti M., Cecchetti M., Di Dato P., Scardi M. and Cataudell S. 2006. Biological monitoring of wild thicklip grey mullet (Chelon

    labrosus), golden grey mullet (Liza aurata), thinlip mullet (Liza ramada) and flathead mullet (Mugil cephalus) (Pisces: Mugilidae) from different Adriatic sites: meristic counts and skeletal anomalies. Ecological Indicators, 6(4): 712–732.

    Cardona L. 2001. Non-competitive coexistence between Mediterranean grey mullet: Evidence from seasonal changes in food availability, niche breadth and trophic overlap. Journal of Fish Biology, 59: 729–744.

    Fent K. 2001. Cytotoxicity of organic environmental chemicals to fish liver cells (PLHC-1). Marine Environmental Research, 42: 377–382.

    Fryer J.L. and Lannan C.N. 1994. Three decades of fish cell culture: A current listing of cell lines derived from fish. Journal of Tissue Culture Methods, 16: 87–94.

    Khamis O. and Hashem M.H. 2012. Developing a cell culture system from Nile tilapia (Oreochromis niloticus, L.) ovarian tissue in Egypt.

  • ()� �*+ � ,-./ 0(1 2��3 ��4 (� 5�.1�� ���6�7� ,89 :6� ������ 6�* � ���������� ��:�� 5)4"( 1396 ]37[

    IOSR Journal of Agriculture and Veterinary Science, 2: 8–12.

    Kumar A., Sharma B. and Pandey R.S. 1998. Assessment of stress in effect to pyrethroid insecticides, λ-cyhalothrin and cypermethrin, in a freshwater fish, Poecilia reticulata (Bloach). Cellular and Molecular Biology Letters, 58: 153–159.

    Lakra W.S., Swaminathan T.R. and Joy K.P. 2011. Development, characterization, conservation and storage of fish cell linesi, a review. Fish Physiology and Biochemistry, 37: 1–20.

    Laffaille P., Feunteun P., Lefebvre C., Radreau A., Sagan G. and Lefeuvre J.C. 2002. Cthin- lipped mullet directly exploit the primary and detritic production of European macrotidal salt marshes? Estuarine, Coastal and Shelf Science, 54(4): 729–739.

    McMaster M.E., Munkittrick K.R., Jardine J.J., Robinson R.D. and Van Der Kraak G.J. 1995. Protocol for measuring in vitro steroid production by fish gonadal tissue. Canadian Technical Report of Fisheries and Aquatic Sciences 1961. 78P.

    Pastor D., Boix J., fernandes V. and Albaiges J. 1996. Bioaccumulation of organachlorinated contaminants in three estuarine fish speciel (Mullus barbatus, Mugil cephalus and Dicentrarchus labrax). Marine pollution Bulletin, 32(3): 257–262.

    Part P., Morrghan L., Bergsrom E. and sjoberg P. 1993. Primary cultures of epithelial cells from rainbow trout gills. Indian Journal of Experimental Biology, 175: 219–233.

    Roy S. and Bhattacharya S. 2016. Arsenic-induced histopathology and synthesis proteins in ovary and kidney of Channa punctatus. Ecotoxicology and Environment Safety, 65(2): 218–229.

    Salamat N., Erfani Majd N., Hashemitabar M., Mesbah M. and Ahangarpoor A. 2010. Isolation of common carp ovarian follicular cells an evalution of their endocrine activity in primary cell culture. Iranian Journal of Fisheries Science, 9(2): 305–314.

    Sunil Kumar G., Bright Singh I.S. and Philip R. 2001. Development of a cell culture system from the ovarian tissue of African catfish (Clarias gariepinus). Aquaculture, 194(1–2): 51–62.

    Wen C.M., Lee C.W., Wang C.S., Cheng Y.H. and Huang H.Y. 2008. Development of two cells from Epinephelus coioides brain tissue for characterization of betanodavirus and megalocytivirus infectivity and propagation. Aquaculture, 278: 14–21.

    Wolf K. and Quimby M.C. 1976. Primary monolayer culture of fish cells initiated from minced tissue.

  • ]38[ �� ������� � ������ :���� ���������� � ����5)4 "(1396

    Tissue Culture Associated Manual, 2(4): 445–448.

    Yilmaz F. 2009. The comparison of heavy metal concentrations (Cd, Cu, Mn, Pb, and Zn) in tissues of three economically important fish (Anguilla anguilla, Mugil cephalus and Oreochromis niloticus) inhabiting, Koycegiz Lake- Mugla

    (Turkey). Turkish Journal of Science and Technology, 4(1): 7–15.

    Zhou B., Liu W., Wu R.S.S. and Lam P.K.S. 2005. Culture gill epithelial cells from tilapia (Oreochromis niloticus): A new in vitro assay for toxicants. Aquatic Toxicology, 71: 61–72.

  • Aquatic Physiology and Biotechnology

    Vol. 5, No. 4, Winter 2018

    The effect of temperature changes and fetal bovine serum concentration on the growth of cultivated ovarian cells of

    Liza klunzingeri

    Zahra Shaibani1,2, Abdolali Movahedinia3,4*, Negin Salamat4, Mahmoud Hashemitabar5, Vahid Bayati6

    Received: December 2016 Accepted: March 2017

    Abstract The aim of this study was to evaluate the effect of fetal bovine serum (FBS)

    and temperature changes on the cultivated ovarian cells of Liza klunzingeri. For this purpose, 10 fish weighing 45 to 50 grams were caught and transferred to the laboratory. After disinfecting the body of the fish with 70% ethanol, the ovarian tissue was removed from the body and divided by scissors into small pieces. The ovarian cells were aspirated by using trypsin enzyme and were cultivated in the flasks containing L-15 medium under different temperature conditions (20, 25, 28, 30 and 32°C) and the serum concentrations of bovine embryos (0, 10, 15 and 20%). Cell count showed the maximum density of L. klunzingeri ovarian cells was observed in 15% FBS concentration, while temperature variations had no significant effect on cell proliferation. In conclusion, the most suitable conditions for the culture of ovarian cells in L. klunzingeri are the use of FBS 15% and temperatures above 20°C.

    Key words: Cell Culture, Liza klunzingeri, FBS, Temperature. 1- Ph.D. Student in Marine Biology, Department of Marine Biology, Faculty of Marine Sciences, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran. 2- Scientific Member in Department of Biology, Payame Noor University, Tehran, Iran. 3- Associate Professor in Department of Marine Biology, Faculty of Marine Sciences, University of Mazandaran, Babolsar, Iran. 4- Associate Professor in Department of Marine Biology, Faculty of Marine Sciences, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran. 5- Associate Professor in Molecular and Cellular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. 6- Assistant Professor in Molecular and Cellular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. *Corresponding Author: [email protected]

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