三沢 典彦 研究業績全目録 平成 26 年 3 月 31 日 現在

＜英文原著論文 (査読付き)＞ 1. Jutori, H., Misawa, N., Ohyama, K., and Komano, T. (1983) Protoplast isolation from soybean and wheat cell

suspension cultures and soybean-wheat intergeneric fusion. Memories of the College of Agriculture, Kyoto University, 121: 35-41.

2. Ohyama, K., Uchida, Y., Misawa, N., Komano, T., Fujita, M., and Ueno, T. (1984) Oil body formation in Euphorbia tirucalli L. cell suspension cultures. Plant Cell Report, 3: 21-22.

3. Ohyama, K., Misawa, N., Yamano, Y., and Komano, T. (1984) Protoplast isolation from Euphorbia tirucalli L. cell suspension cultures and sustained cell division. Z. Pflanzenphysiol., 113: 367-370.

4. Misawa, N., Nakamura, K., and Kitamura, K. (1985) Three 1.7 kilobase pair plasmids in Zymomonas mobilis NRRL B-806. Agric. Biol. Chem., 49: 2769-2771.

5. Nakamura, K., Misawa, N., and Kitamura, K. (1986) Cellulase genes of Cellulomonas uda CB4. I. Cloning and

expression of β-glucosidase genes in Escherichia coli. J. Biotechnol., 3: 239-246. 6. Nakamura, K., Misawa, N., and Kitamura, K. (1986) Cellulase genes of Cellulomonas uda CB4. II. Cloning

and expression of a CM-cellulose hydrolyzing enzyme (endoglucanase) gene in Escherichia coli. J. Biotechnol., 3: 247-253.

7. Nakamura, K., Misawa, N., and Kitamura, K. (1986) Sequence of a cellulase gene of Cellulomonas uda CB4. J. Biotechnol., 4: 247-254.

8. Misawa, N., Okamoto, T., Nakamura, K., Kitamura, K., Yanase, H., and Tonomura, K. (1986) Construction of a new shuttle vector for Zymomonas mobilis. Agric. Biol. Chem., 50: 3201-3203.

9. Misawa, N., Okamoto, T., and Nakamura, K. (1988) Expression of a cellulase gene in Zymomonas mobilis. J. Biotechnol., 7: 167-177.

10. Misawa, N., and Nakamura, K. (1989) Expression and stability of a β-glucosidase gene of Ruminococcus albus in Zymomonas mobilis. Agric. Biol. Chem., 53: 723-727.

11. Misawa, N., and Nakamura, K. (1989) Nucleotide sequence of the 2.7 kb plasmid of Zymomonas mobilis ATCC10988. J. Biotechnol., 12: 63-70.

12. Misawa, N., Nakagawa, M., Kobayashi, K., Yamano, S., Izawa, Y., Nakamura, K., and Harashima, K. (1990) Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli. J. Bacteriol., 172 (12): 6704-6712.

13. Misawa, N., Yamano, S., and Ikenaga, H. (1991) Production of β-carotene in Zymomonas mobilis and Agrobacterium tumefaciens by introduction of the biosynthesis genes from Erwinia uredovora. Appl. Environ. Microbiol., 57: 1847-1849.

14. Nakagawa, M., and Misawa, N. (1991) Analysis of carotenoid glycosides produced in gram-negative bacteria by introduction of the Erwinia uredovora carotenoid biosynthesis genes. Agric. Biol. Chem., 55: 2147-2148.

15. Linden, H., Misawa, N., Chamovitz, D., Pecker, I., Hirschberg, J., and Sandmann, G. (1991) Functional complementation in Escherichia coli of different phytoene desaturase genes and analysis of accumulated carotenes. Z. Naturforsch., 46c: 1045-1051.

16. Sandmann, G., and Misawa, N. (1992) New functional assignment of the carotenogenic genes crtB and crtE

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with constructs of these genes from Erwinia species. FEMS Microbiol. Lett., 90: 253-258. 17. Chamovitz, D., Misawa, N., Sandmann, G., and Hirschberg, J. (1992) Molecular cloning and expression in

Escherichia coli of a cyanobacterial gene coding for phytoene synthase, a carotenoid biosynthesis enzyme. FEBS Lett., 296: 305-310.

18. Fraser, P. D., Misawa, N., Linden, H., Yamano, S., Kobayashi, K., and Sandmann, G. (1992) Expression in Escherichia coli, purification and reactivation of the recombinant Erwinia uredovora phytoene desturase. J. Biol. Chem., 267 (28): 19891-19895.

19. Sandmann, G., Misawa, N., Wiedemann, M., Vittorioso, P., Carattoli, A., Morelli, G., and Macino, G. (1993) Functional identification of al-3 from Neurospora crassa as the gene for geranylgeranyl pyrophosphate synthase by complementation with crt genes, in vitro characterization of the gene product and mutant analysis.

J. Photochem. Photobiol. B: Biol., 18: 245-251. 20. Cunningham, F. X., Chamovitz, D., Misawa, N., Gantt, E., and Hirschberg, H. (1993) Cloning and functional

expression in Escherichia coli of a cyanobacterial gene for lycopene cyclase, the enzyme that catalyzes the

biosynthesis of β-carotene. FEBS Lett., 328: 130-138. 21. Wiedemann, M., Misawa, N., and Sandmann, G. (1993) Purification and enzymatic characterization of the

geranylgeranyl pyrophosphate synthase from Erwinia uredovora after expression in Escherichia coli. Arch.

Biochem. Biophys., 306: 152-157. 22. Misawa, N., Yamano, S., Linden, H., De Felipe, M. R., Lucas, M., Ikenaga, H., and Sandmann, G. (1993)

Functional expression of the Erwinia uredovora carotenoid biosynthesis gene crtI in transgenic plants showing

an increase of β-carotene biosynthesis activity and resistance to the bleaching herbicide norflurazon. Plant J., 4 (5): 833-840.

23. Linden, H., Misawa, N., Saito, T., and Sandmann, G. (1994) A novel carotenoid biosynthesis gene coding for

ζ-carotene desaturase: functional expression, sequence and phylogenetic origin. Plant Mol. Biol., 24: 369-379. 24. Yamano, S., Ishii, T., Nakagawa, M., Ikenaga, H., Misawa, N. (1994) Metabolic engineering for production of

β-carotene and lycopene in Saccharomyces cerevisiae. Biosci. Biotech. Biochem., 58: 1112-1114. 25. Misawa, N., Masamoto, K., Hori, T., Ohtani, T., Böger, P., and Sandmann, G. (1994) Expression of an Erwinia

phytoene desaturase gene not only confers multiple resistance to herbicides interfering with carotenoid biosynthesis but also alters xanthophyll metabolism in transgenic plants. Plant J., 6 (4): 481-489.

26. Misawa, N., Truesdale, M. R., Sandmann, G., Fraser, P. D., Bird, C., Schuch, W., and Bramley, P. M. (1994) Expression of a tomato cDNA coding for phytoene synthase in Escherichia coli, phytoene formation in vivo and in vitro, and functional analysis of the various truncated gene products. J. Biochem., 116: 980-985.

27. Misawa, N., Kajiwara, S., Kondo, K., Yokoyama, A., Satomi, Y., Saito, T., Miki, W., and Ohtani, T. (1995)

Canthaxanthin biosynthesis by the conversion of methylene to keto groups in a hydrocarbon β-carotene by a single gene. Biochem. Biophy. Res. Com., 209: 867-876.

28. Satomi, Y., Yoshida, T., Aoki, K., Misawa, N., Masuda, M., Murakoshi, M., Takasuka, N., Sugimura, T., and Nishino, H. (1995) Production of phytoene, an oxidative stress protective carotenoid, in mammalian cells by introduction of phytoene synthase gene crtB isolated from a bacterium Erwinia uredovora. Proc. Japan Acad., 71, Ser. B: 236-240.

29. Kajiwara, S., Kakizono, T., Saito, T., Kondo, K., Ohtani, T., Nishio, N., Nagai, S., and Misawa, N. (1995) Isolation and functional identification of a novel cDNA for astaxanthin biosynthesis from Haematococcus

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pluvialis, and astaxanthin synthesis in Escherichia coli. Plant Mol. Biol., 29: 343-352. 30. Misawa, N., Satomi, Y., Kondo, K., Yokoyama, A., Kajiwara, S., Saito, T., Ohtani, T., and Miki, W. (1995)

Structure and functional analysis of a marine bacterial carotenoid biosynthesis gene cluster and astaxanthin biosynthetic pathway proposed at the gene level. J. Bacteriol., 177 (22): 6575-6584.

31. Kondo, K., Saito, T., Kajiwara, S., Takagi, M., and Misawa, N. (1995) A transformation system for the yeast Candida utilis: use of a modified endogenous ribosomal protein gene as a drug-resistant marker and ribosomal DNA as an integration target for vector DNA. J. Bacteriol., 177: 7171-7177.

32. Schnurr, G., Misawa, N., and Sandmann, G. (1996) Expression, purification and properties of lycopene cyclase from Erwinia uredovora. Biochem. J. 315: 869-874.

33. Breitenbach, J., Misawa, N., Kajiwara, S., and Sandman, G. (1996) Expression in Escherichia coli and properties of the carotene ketolase from Haematococcus pluvialis. FEMS Microbiol. Lett., 140: 241-246.

34. Takaichi, S., Sandmann, G., Schnurr, G., Satomi, Y., Suzuki, A., and Misawa, N. (1996) The carotenoid

7,8-dihydro-ψ-end group can be cyclized by the lycopene cyclases from the bacterium Erwinia uredovora and the higher plant Capsicum annuum. Eur. J. Biochem., 241: 291-296.

35. Fraser, P. D., Miura, Y., and Misawa, N. (1997) In vitro characterization of astaxanthin biosynthetic enzymes. J. Biol. Chem., 272:6128-6135.

36. Kajiwara, S., Fraser, P. D., Kondo, K., and Misawa, N. (1997) Expression of an exogenous isopentenyl diphosphate isomerase gene enhances isoprenoid biosynthesis in Escherichia coli. Biochem. J., 324: 421-426.

37. Ruther, A., Misawa, N., Boger, P., and Sandmann, G. (1997) Production of zeaxanthin in Escherichia coli transformed with different carotenogenic plasmids. Appl. Microbiol. Biotech., 48: 162-167.

38. Albrecht, M., Takaichi, S., Misawa, N., Schnurr, G., Boger, P., and Sandmann, G. (1997) Synthesis of atypical cyclic and acyclic hydroxy carotenoids in Escherichia coli transformants. J. Biotechnol., 58: 177-185.

39. Miura, Y., Kondo, K., Shimada, H., Saito, T., Nakamura, K., and Misawa, N. (1998) Production of lycopene by the food yeast, Candida utilis that does not naturally synthesize carotenoid. Biotechnol. Bioeng., 58: 306-308.

40. Fraser, P. D., Shimada, H., and Misawa, N. (1998) Enzymic confirmation of reactions involved in routes to astaxanthin formation, elucidated using a direct substrate in vitro assay. Eur. J. Biochem., 252: 229-236.

41. Miura, Y., Kondo, K., Saito, T., Shimada, H., Fraser. P. D., and Misawa, N. (1998) Production of the

carotenoids lycopene, β-carotene, and astaxanthin in the food yeast Candida utilis. Appl. Environ. Microbiol., 64: 1226-1229.

42. Yokoyama, A., Shizuri, Y., and Misawa, N. (1998) Production of new carotenoids, astaxanthin glucosides, by Escherichia coli transformants carrying carotenoid biosynthetic genes. Tetrahedron Lett., 39: 3709-3712.

43. Masamoto, K., Misawa, N., Kaneko, T., Kikuno, R., and Toh, H. (1998) β-carotene hydroxylase gene from the cyanobacterium Synechocystis sp. strain PCC6803. Plant Cell Physiol., 39: 560-564.

44. Shimada, H., Kondo, K., Fraser, P. D., Miura, Y., Saito, T., and Misawa, N. (1998) Increased carotenoid production by the food yeast Candida utilis through metabolic engineering of the isoprenoid pathway. Appl. Environ. Microbiol., 64: 2676-2680.

45. Fraser, P. D., Misawa, N., Sandmann, G., Johnson, J., Schuch, W., and Bramley, P. M. (1998) Production and characterization of monoclonal antibodies to phytoene synthase of Lycopersicom esculentum. Phytochemistry, 49: 971-978.

46. Tatsuzawa, H., Maruyama, T., Misawa, N., Fujimori, K., Hori, K., Sano, Y., Kambayashi, Y., and Nakano, M.

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(1998) Inactivation of bacterial respiratory chain enzymes by singlet oxygen. FEBS Lett., 439: 329-333. 47. Verdoes, J. C., Misawa, N., and van Ooyen, A. J. J. (1999) Cloning and characterization of the astaxanthin

biosynthetic gene encoding phytoene desaturase of Xanthophyllomyces dendrorhous. Biotechnol. Bioeng., 63: 750-755.

48. Arimochi, H., Kataoka, K., Kuwahara, T., Nakayama, H., Misawa, N., and Ohnishi, Y. (1999) Effects of

β-glucuronidase-deficient and lycopene-producing Escherichia coli stranis on formation of azoxymethane-induced aberrant crypt foci in the rat colon. Biochem. Biophy, Res. Com., 262: 322-327.

49. Albrecht, M., Misawa, N., and Sandmann, G. (1999) Metabolic engineering of the terpenoid biosynthetic

pathway of Escherichia coli for production of the carotenoids β-carotene and zeaxanthin. Biotechnol. Lett., 21: 791-795.

50. Römer, S, Fraser, P. D., Kiano, J. W., Shipton, C. A., Misawa, N., Schuch, W., and Bramley, P. M. (2000) Elevation of the provitamin A content of transgenic tomato plants. Nature Biotechnol., 18: 666-669.

51. Tatsuzawa, H., Maruyama, T., Misawa, N., Fujimori, K., and Nakano, M. (2000) Quenching of singlet oxygen by carotenoids produced in Escherichia coli - attenuation of singlet oxygen-mediated bacterial killing by carotenoids. FEBS Lett., 484: 280-284.

52. Kakinuma, K., Dekishima, Y., Matsushima, Y., Eguchi, T., Misawa, N., Takagi, M., Kuzuyama, T., and Seto, H. (2001) New approach to multiply deuterated isoprenoids using triply engineered Escherichia coli and its potential as a tool for mechanistic enzymology. J. Am. Chem. Soc., 123: 1238-1239.

53. Chun, H-. K., Ohnishi, Y., Misawa, N., Shindo, K., Hayashi, M., Harayama, S., and Horinouchi, S. (2001) Biotransformation of phenanthrene and 1-methoxynaphthalene with Streptomyces lividans cells expressing a marine bacterial phenanthrene dioxygenase gene cluster. Biosci. Biotechnol. Biochem., 65: 1774-1781.

54. Harada, J., Nagashima, K., Takaichi, S., Misawa, N., Matsuura, K., and Shimada, K. (2001) Phytoene desaturase, CrtI, of the purple photosynthetic bacterium, Rubrivivax gelatinosus, produces both neurosporene and lycopene. Plant Cell Physiol., 42: 1112-1118.

55. Shindo, K., Ohnishi, Y., Chun, H-. K., Takahashi, H., Hayashi, M., Saito, A., Iguchi, K., Furukawa, K., Harayama, S., Horinouchi, S., and Misawa, N. (2001) Oxygenation reactions of various tricyclic fused aromatic compounds using Escherichia coli and Streptomyces lividans transformants carrying several arene dioxygenase genes. Biosci. Biotechnol. Biochem., 65: 2472-2481.

56. Fraser, P. D., Römer, S., Shipton, C. A., Mills, P. B., Kiano, J. W., Misawa, N., Drake, R. G., Schuch, W., and Bramley, P. M. (2002) Evaluation of transgenic tomato plants expressing an additional phytoene synthase in a fruit-specific manner. Proc. Natl. Acad. Sci. USA, 99: 1092-1097.

57. Misawa, N., Shindo, K., Takahashi, H., Suenaga, H., Iguchi, K., Okazaki, H., Harayama, S., and Furukawa, K. (2002) Hydroxylation of various molecules including heterocyclic aromatics using recombinant Escherichia coli cells expressing modified biphenyl dioxygenase genes. Tetrahedron, 58: 9605-9612.

58. Chun, H-. K., Ohnishi, Y., Shindo, K., Misawa, N., Furukawa, K., and Horinouchi, S. (2003) Biotransformation of flavone and flavanone by Streptomyces lividans cells carrying shuffled biphenyl dioxygenase genes. J. Mol. Catalysis B: Enzymatic, 21: 113-121.

59. Shindo, K., Nakamura, R., Chinda, I., Ohnishi, Y., Horinouchi, S., Takahashi, H., Iguchi, K., Harayama, S., Furukawa, K., and Misawa, N. (2003) Hydroxylation of ionized aromatics including carboxylic acid or amine using recombinant Streptomyces lividans cells expressing modified biphenyl dioxygenase genes. Tetrahedron,

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59: 1895-1900. 60. Peng, X., Misawa, N., and Harayama, S. (2003) Isolation and characterization of thermophilic Bacilli

degrading cinnamic, 4-coumaric, and ferulic acids. Appl. Environ. Microbiol., 69: 1417-1427. 61. Shindo, K., Kagiyama, Y., Nakamura, R., Hara, A., Ikenaga, H., Furukawa, K., and Misawa, N. (2003)

Eyzymatic synthesis of novel antioxidant flavonoids by Escherichia coli cells expressing modified metabolic genes involved in biphenyl catabolism. J. Mol. Catalysis B: Enzymatic, 23: 9-16.

62. Teramoto, M., Takaichi, S., Inomata, Y., Ikenaga, H., and Misawa, N. (2003) Structural and functional analysis

of a lycopene β-monocyclase gene isolated from a unique marine bacterium that produces myxol. FEBS Lett., 545: 120-126.

63. Kasai, Y., Shindo, K., Harayama, S., and Misawa, N. (2003) Molecular characterization and substrate preference of polycyclic aromatic hydrocarbon dioxygenase from Cycloclasticus sp. strain A5. Appl. Environ. Microbiol., 69: 6688-6697.

64. Hara, A., Baik, S.-H., Syutsubo, K., Misawa, N., Smits, H. M., Beilen, J. B. van, and Harayama, S. (2004) Cloning and functional analysis of alkB genes in Alcanivorax borkumensis SK2. Environ. Microbiol., 6: 191-197.

65. Kasai, H., and Misawa, N. (2004) Genetic diversity of carotenogenic marine bacteria revealed by crtI. Carotenoid Sci., 7: 54-56.

66. Misawa, N., Teramoto, M., Choi, S.-K., and Adachi, K. (2004) Development of technologies for controlling the production of carotenoids in plants. –Introduction of NEDO project for plant biotechnology-. Carotenoid Sci., 7: 57-59.

67. Fuse, M., Tanaka, T., Shibata, T., Yoshida, T., Noguchi, Y., Misawa, N., Yasuda, T., Saito, Y., Kohn, L. D., and Tatsuno, I. (2004) Regulation of geranylgeranyl pyrophosphate synthase in the proliferation of rat FRTL-5

cells: involvement of both cAMP-PKA and PI3-AKT pathways. Biochem. Biophys. Res. Commum., 315: 1147-1153.

68. Ralley, L., Enfissi, E.M.A., Misawa, N., Schuch, W., Bramley, P.M., and Fraser, P.D. (2004) Metabolic engineering of ketocarotenoid formation in higher plants. Plant J., 39: 477-486.

69. Teramoto, M., Rahlert, N., and Misawa, N., and Sandmann, G. (2004) 1-Hydroxy monocyclic carotenoid 3,4-dehydrogenase from a marine bacterium that produces myxol. FEBS Lett., 570: 184-188.

70. Satomi, Y., Misawa, N., Maoka, T., and Nishino, H. (2004) Production of phytoene, a carotenoid, and induction of connexin 26 in transgenic mice carrying the phytoene synthase gene crtB. Biochem. Biophysi. Res. Comm., 320: 398-401.

71. Wang, J. -Q., Kon, J., Mogi, C., Tobo, M., Damirin, A., Sato, K., Komachi, M., Malchinkhuu, E. Murata, N., Kimura, T., Kuwabara, A., Wakamatsu, K., Koizumi, H., Uede, T., Tsujimoto, G., Kurose, H., Sato, T., Harada, A., Misawa, N., Tomura, H., and Okajima, F., (2004) TDAG8 is a proton-sensing and psychosine-sensitive G-protein-coupled receptor. J. Biol. Chem., 279: 45626-45633.

72. Shindo, K., Osawa, A., Nakamura, R., Kagiyama, Y., Sakuda, S., Shizuri, Y., Furukawa, K., and Misawa, N. (2004) Conversion from arenes having a benzene ring to those having a picolinic acid by simple growing cell reactions using Escherichia coli that expressed the six bacterial genes involved in biphenyl catabolism. J. Am. Chem. Soc., 126: 15042-15043.

73. Misawa, N., Nakamura, R., Kagiyama, Y., Ikenaga, H., Furukawa, K., and Shindo, K. (2005) Synthesis of

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vicinal diols from various arenes with a heterocyclic, amino or carboxyl group by using recombinant Escherichia coli cells expressing evolved biphenyl dioxygenase and dihydrodiol dehydrogenase genes. Tetrahedron, 61: 195-204.

74. Eguchi, T., Dekishima, Y., Aizawa, H., Tamegai, H., Kakinuma, K., Misawa, N., Kuzuyama, T., and Seto, H.

(2005) Preparation of highly deuterated zeaxanthin, lycopene, and β-carotene from fully deuterated mevalonate using engineered Escherichia coli. Tetrahedron, 61: 2027-2035.

75. Tsuchiya, T., Takaichi, S., Misawa, N., Maoka, T., Miyashita, H., and Mimuro, M. (2005). The cyanobacterium Gloeobacter violaceus PCC 7421 uses bacterial-type phytoene desaturase in carotenoid biosynthesis, FEBS Lett., 579: 2125-2129.

76. Tokimatsu, T., Sakurai, N., Suzuki, H., Ohta, H., Nishitani, K., Koyama, T., Umezawa, T., Misawa, N., Saito, K., and Shibata, D. (2005) KaPPA-View. A web-based analysis tool for integration of transcript and metabolite data on plant metabolic pathway maps. Plant Physiol., 138: 1289-1300.

77. Nishida, Y., Adachi, K., Kasai, H., Shizuri, Y., Shindo, K., Sawabe, A., Komemushi, S., Miki, W., and Misawa,

N. (2005) Elucidation of a carotenoid biosynthesis gene cluster encoding a novel enzyme, 2,2’-β-hydroxylase, from Brevundimonas sp. strain SD212 and combinatorial biosynthesis of new or rare xanthophylls. Appl. Environ. Microbiol., 71 (8): 4286-4296.

78. Shindo, K., Nakamura, R., Osawa, A., Kagami, O., Kanoh, K., Furukawa, K., and Misawa, N. (2005) Biocatalytic synthesis of monocyclic arene-dihydrodiols and -diols by Escherichia coli cells expressing hybrid toluene/biphenyl dioxygenase and dihydrodiol dehydrogenase genes. J. Mol. Catalysis B: Enzymatic, 35: 134-141.

79. Choi, S. –K., Nishida, Y., Matsuda, S., Adachi, K., Kasai, H., Peng, X., Komemushi, S., Miki, W., and Misawa,

N. (2005) Characterization of β-carotene ketolases, CrtW, from marine bacteria by complementation analysis in Escherichia coli, Marine Biotechnol., 7: 515-522.

80. Tomura, H., Wang. J. Q., Komachi, M., Damirin, A., Mogi, C., Tobo, M., Kon, J., Misawa, N., Sato, K., and Okajima, F. (2005) Prostaglandin I2 production and cAMP accumulation in response to acidic extracellular pH through OGR1 in human aortic smooth muscle cells. J. Biol. Chem., 280: 34458-34464.

81. Maruyama, T., Ishikura, M, Taki, H., Shindo, K., Kasai, H., Haga, M., Inomata, Y., and Misawa, N. (2005) Isolation and characterization of o-xylene oxygenase genes from Rhodococcus opacus TKN14. Appl. Environ.

Microbiol., 71: 7705-7715. 82. Peng, X., Shindo, K., Kanoh, K., Inomata, Y., Choi, S. -K., and Misawa, N. (2005) Characterization of

Sphingomonas aldehyde dehydrogenase catalyzing the conversion of various aromatic aldehydes to their carboxylic acids. Appl. Microbiol. Biotechnol., 69: 141-150.

83. Kubota, M., Nodate, M., Yasumoto-Hirose, M., Uchiyama, T., Kagami, O., Shizuri, Y., and Misawa, N. (2005) Isolation and functional analysis of cytochrome P450 CYP153A genes from various environments. Biosci.

Biotechnol. Biochem., 69: 2421-2430. 84. Shibata, T., Misawa, N., Takeo, C., Saeki, N., Saito, Y., and Tatsuno, I. (2005) Analysis of genes dominantly

expressed in rat cerebral endothelial cells using suppression subtractive hybridization. J. Atheroscler Thromb., 12: 330-337.

85. Tao, L., Yao, H., Kasai, H., Misawa, N., and Cheng, Q. (2006) A carotenoid synthesis gene cluster from

Algoriphagus sp. KK10202C with a novel fusion-type lycopene-β-cyclase gene. Mol. Gen. Genom., 276:

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79-86. 86. Nodate, M., Kubota, M., and Misawa, N. (2006) Functional expression system for cytochrome P450 genes

using the reductase domain of self-sufficient P450RhF from Rhodococcus sp. NCIMB 9784. Appl. Microbiol. Biotechnol., 71: 455-462.

87. Peng, X., Adachi, K., Chen, C., Kasai, H., Kanoh, K., Shizuri, Y., and Misawa, N. (2006) Discovery of a marine bacterium producing 4-hydroxybenzoate and its alkyl esters, parabens. Appl. Environ. Microbiol., 72: 5556-5561.

88. Peng, X., Taki, H., Komukai, S., Sekine, M., Kanoh, K., Kasai, H., Choi, S. -K., Omata, S., Tanikawa, S., Harayama, S., and Misawa, N. (2006) Characterization of four Rhodococcus alcohol dehydrogenase genes responsible for the oxidation of aromatic alcohols. Appl. Microbiol. Biotechnol., 71: 824-832.

89. Choi, S. –K., Matsuda, S., Hoshino, T., Peng, X., and Misawa, N. (2006) Characterization of bacterial

β-carotene 3,3’-hydroxylases, CrtZ, and P450 in astaxanthin biosynthetic pathway and adonirubin production by gene combination in Escherichia coli. Appl. Microbiol. Biotechnol., 72: 1238-1246.

90. Adachi, K., Katsuta, A., Matsuda, S., Peng, X., Misawa, N., Shizuri, Y., Kroppenstedt, R.M., Yokota, A., and Kasai, H. (2007) Smaragdicoccus niigatensis gen. nov, sp. nov., a novel member of the suborder Corynebacterineae. Int. J. Syst. Evol. Microbiol. 57: 297-301.

91. Shindo, K., Mikami, K., Tamesada, E., Takaichi, S., Adachi, K., Misawa, N., and Maoka, T. (2007) Diapolycopenedioc acid xylosyl ester, a novel glyco-C30-carotenoic acid produced by a new marine bacterium Rubritalea squalenifaciens. Tetrahedron Lett., 48: 2725-2727.

92. Shindo, K., Kikuta, K., Suzuki, A., Katsuta, A., Kasai, H., Yasumoto-Hirose, M., Matuo, Y., Misawa, N., and Takaichi, S. (2007) Rare carotenoids, (3R)-Saproxanthin and (3R,2’S)-myxol, isolated from novel marine bacteria (Flavobacteriaceae) and their antioxidant activities. Appl. Microbiol. Biotechnol., 74: 1350-1357.

93. Shindo, K., Shindo, Y., Hasegawa, T., Osawa, A., Kagami, O., Furukawa, K., and Misawa, N. (2007) Synthesis of highly hydroxylated aromatics by evolved biphenyl dioxygenase and subsequent dihydrodiol dehydrogenase. Appl. Microbiol. Biotechnol., 75: 1063-1069.

94. Choi, S. –K., Harada, H., Matsuda, S., and Misawa, N. (2007) Characterization of two β-carotene ketolases, CrtO and CrtW, by complementation analysis in Escherichia coli. Appl. Microbiol. Biotechnol., 75: 1335-1341.

95. Suzuki, S., Nishihara, M., Nakatsuka, T., Misawa, N., Ogiwara, I., and Yamamura, S. (2007) Flower color alteration in Lotus japonicus by modification of the carotenoid biosynthetic pathway. Plant Cell Rep., 26: 951-959.

96. Ramasamy, A. K., Choi, S. -K, Udayasuriyan, V., and Misawa, N. (2007) Structure of a carotenoid gene cluster from Pantoea sp. strain C1B1Y and characterization of β-carotene hydroxylase (crtZ) gene by functional complementation in Escherichia coli. Carotenoid Sci., 11: 10-15.

97. Maruyama, T., Kasai, H., Choi, S. -K, Ramasamy, A. K., Inomata, Y., and Misawa, N. (2007) Structure of a complete carotenoid biosynthesis gene cluster of marine bacterium Paracoccus sp. strain N81106. Carotenoid Sci., 11: 50-55.

98. Shindo, K., Osawa, A., Kasai, Y., Iba, N., Saotome, A., and Misawa, N. (2007) Hydroxylations of substituted naphthalenes by Escherichia coli expressing aromatic dihydroxylating dioxygenase genes from polycyclic aromatic hydrocarbon-utilizing marine bacteria. J. Mol. Catalysis B: Enzymatic, 48: 77-83.

99. Yu, F., Harada, H., Yamazaki, K., Okamoto, S., Hirase, S., Tanaka, Y., Misawa, N., and Utsumi, R. (2008)

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Isolation and functional characterization of a β-eudesmol synthase, a new sesquiterpene synthase from Zingiber zerumbet Smith. FEBS Lett., 582: 565-572.

100. Yu, F., Okamoto, S., Nakasone, K., Adachi, K., Matsuda, S., Harada, H., Misawa, N., and Utsumi, R. (2008)

Molecular cloning and functional characterization of α-humulene synthase, a possible key enzyme of zerumbone biosynthesis in shampoo ginger (Zingiber zerumbet Smith). Planta, 227: 1291-1299.

101. Shindo, K., Asagi, E., Sano, A., Hotta, Y., Minemura, N., Mikami, K., Tamesada, E., Misawa, N., and Maoka, T. (2008) Diapolycopenedioic acid xylosyl esters A, B, and C, novel antioxidative glyco-C30-carotenoic acids produced by a new marine bacterium Rubritalea squalenifaciens. J. Antibiotics, 61: 185-191.

102. Shindo, K., Hasunuma, T., Asagi, E., Sano, A., Hotta, E., Minemura, N., Miyake, C., Maoka, T., and Misawa, N. (2008) 4-Ketoantheraxanthin, a novel carotenoid produced by the combination of the bacterial enzyme

β-carotene ketolase CrtW and endogenous carotenoid biosynthetic enzymes in higher plants. Tetrahedron Lett., 49: 3294-3296.

103. Fujisawa, M., Watanabe, M., Choi, S. –K., Teramoto, M., Ohyama, K., and Misawa, N. (2008) Enrichment of carotenoids in flaxseed (Linum usitatissimum) by metabolic engineering with introduction of bacterial phytoene synthase gene crtB. J. Biosci. Bioeng., 105: 636-641.

104. Kagami, O., Shindo, K., Kyojima, A., Takeda, K., Ikenaga, H., Furukawa, K., and Misawa, N. (2008) Protein engineering on biphenyl dioxygenase for conferring activity to convert 7-hydroxyflavone and 5,7-dihydroxyflavone (chrysin). J. Biosci. Bioeng., 106: 121-127.

105. Hasunuma, T., Miyazawa, S., Yoshimura, S., Shinzaki, Y., Tomizawa, K., Shindo, K., Choi, S. –K., Misawa, N., and Miyake, C. (2008) Biosynthesis of astaxanthin in tobacco leaves by transplastomic engineering. Plant J., 55: 857-868.

106. Makino, T., Harada, H., Ikenaga, H., Matsuda, S., Takaichi, S., Shindo, K., Sandmann, G., Ogata, T., and Misawa, N. (2008) Characterization of cyanobacterial carotenoid ketolase CrtW and hydroxylase CrtR by complementation analysis in Escherichia coli. Plant Cell Physiol., 49: 1867-1878.

107. Rahim, M. A., Nakajima, A., Misawa, N., Shindo, K., Adachi, K., Shizuri, Y., Ohizumi, Y., and Yamakuni, T. (2008) A novel diol-derivative of chalcone produced by bioconversion, 3-(2,3-dihydroxyphenyl)-1-phenylpropan-1-one, activates PKA/MEK/ERK signaling and antagonizes

Aβ-inhibion of the cascade in cultured rat CNS neurons. Eur. J. Pharmacol., 600: 10-17. 108. Shindo, K., Endo, M., Miyake, Y., Wakasugi, K., Morritt, D., Bramley, P. M., Fraser, P. D., Kasai, H., and

Misawa, N. (2008) Methyl glucosyl-3,4-dehydro-apo-8’-lycopenoate, a novel antioxidative glyco-C30-carotenoic acid produced by a marine bacterium Planococcus maritimus. J. Antibiotics, 61: 729-735.

109. Harada, H., Yu, F., Okamoto, S., Kuzuyama, T., Utsumi, R., and Misawa, N. (2009) Efficient synthesis of functional isoprenoids from acetoacetate through metabolic pathway-engineered Escherichia coli. Appl. Microbiol. Biotechnol., 81: 915-925.

110. Fujisawa, M., Takita, E., Harada, H., Sakurai, N., Suzuki, H., Ohyama, K., Shibata, D., and Misawa, N. (2009) Pathway engineering of Brassica napus seeds using multiple key-enzyme genes involved in ketocarotenoid formation. J. Exp. Bot., 60 (4): 1319-1332.

111. Harada, H., Fujisawa, M., Teramoto, M., Sakurai, N., Suzuki, H., Shibata, D., and Misawa, N. (2009) Simple functional analysis of key genes involved in astaxanthin biosynthesis using Arabidopsis cultured cells. Plant Biotechnol., 26: 81-92.

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112. Fujita, N., Sumisa, F., Shindo, K., Kabumoto, H., Arisawa, A., Ikenaga, H., and Misawa, N. (2009) Comparison of two vectors for functional expression of a bacterial cytochrome P450 gene in Escherichia coli using CYP153 genes. Biosci. Biotechnol. Biochem., 73: 1825-1830.

113. Gotoh,Y., Doi, A., Furuta, E., Dubrac, S., Ishizaki, Y., Okada, M., Igarashi, M., Misawa, N., Yoshikawa, H., Okajima, T., Msadek, T., and Utsumi, R. (2010) Novel antibacterial compounds specifically targeting the essential WalR response regulator. J. Antibiotics, 63: 127-134.

114. Fujisawa, M., Harada, H., Kenmoku, H., Mizutani, S, and Misawa, N. (2010) Cloning and characterization of a novel gene that encodes (S)-β-bisabolene synthase from ginger, Zingiber officinale. Planta, 232: 121-130; Erratum, 232: 131.

115. Otomatsu, T., Bai, L., Fujita, N., Shindo, K., Shimizu, K., and Misawa, N. (2010) Bioconversion of aromatic compounds by Escherichia coli that expresses cytochrome P450 CYP153A13a gene isolated from an alkane-assimilating marine bacterium Alcanivorax borkumensis. J. Mol. Catalysis B:Enzymatic, 66: 234-240.

116. Azadi, P., Otang, N.V., Chin, D.P., Nakamura, I., Fujisawa, M., Harada, H., Misawa, N., and Mii, M. (2010) Metabolic engineering of Lilium x formolongi using multiple genes of the carotenoid biosynthesis pathway. Plant Biotechnol. Rep.,4: 269-280.

117. Yu, F., Okamoto, S., Harada, H., Yamasaki, K., Misawa, N., and Utsumi, R. (2011) Zingiber zerumbet CYP71BA1 catalyzes the conversion of α-humulene to 8-hydroxy-α-humulene in zerumbone biosynthesis. Cell. Mol. Life Sci., 68: 1033-1040.

118. Misawa, N., Nodate, M., Otomatsu, T., Shimizu, K., Kaido, C., Kikuta, M., Ideno, A., Ikenaga, H., Ogawa, J., Shimizu, S., and Shindo, K. (2011) Bioconversion of substituted naphthalenes and β-eudesmol with the cytochrome P450 BM3 variant F87V. Appl. Microbiol. Biotechnol., 90 (1): 147-157.

119. Harada, H., Shindo, K., Iki, K., Teraoka, A., Okamoto, S., Yu, F., Utsumi, R., Hattan, J., and Misawa, N. (2011) Efficient functional analysis system for cyanobacterial or plant cytochromes P450 involved in sesquiterpene biosynthesis. Appl. Microbiol. Biotechnol., 90 (2): 467-476.

120. Shindo, K., Tachibana, A., Tanaka, A., Toba, S., Yuki, E., Ozaki, T., Kumano, T., Nishiyama, M., Misawa, N., and Kuzuyama, T. (2011) Production of novel antioxidative prenyl naphthalen-ols by combinational bioconversion with dioxygenase PhnA1A2A3A4 and prenyltransferase NphB or SCO7190. Biosci. Biotechnol. Biochem., 75 (3): 505-510.

121. Osawa, A., Harada, H., Choi, S.-K., Misawa, N., and Shindo, K. (2011) Production of caloxanthin

3’-β-D-glucoside, zeaxanthin β-D-diglucoside, and nostoxanthin in a recombinant Escherichia coli expressing system harboring seven carotenoid biosynthesis genes, including crtX and crtG. Phytochem., 72: 711-716.

122. Okamoto, S., Yu, F., Harada, H., Okajima, T., Hattan, J., Misawa, N., and Utsumi, R. (2011) A short-chain dehydrogenase involved in terpene metabolism from Zingiber zerumbet. FEBS J., 278: 2892-2900.

123. Makino, T., Otomatsu, T., Shindo, K., Kitamura, E., Sandmann, G., Harada, H., and Misawa, N. (2012) Biocatalytic synthesis of flavones and hydroxyl-small molecules by recombinant Escherichia coli cells expressing the cyanobacterial CYP110E1 gene. Microbial Cell Factories, 11: 95.

124. Ichige, M., Fukuda, E., Miida, S., Hattan, J., Misawa, N., Saito, S., Fujimaki, T., Imoto, M., and Shindo, K. (2013) Novel isoflavone glucosides in groundnut (Apios americana Medik) and their antiandrogenic activities. J. Agric. Food Chem., 61 (9): 2183-2187.

125. Kitamura, E., Otomatsu,T., Maeda, C., Aoki, Y., Ota, C., Misawa, N., and Shindo K. (2013) Production of

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hydroxlated flavonoids with the cytochrome P450 BM3 variant F87V and their antioxidative activities. Biosci. Biotechnol. Biochem., 77 (6): 1340-1343.

126. Choi, S. –K., Osawa, A., Maoka, T., Hattan, J., Ito, K., Uchiyama, A., Suzuki, M., Shindo, K., and Misawa, N. (2013) 3-β-Glucosyl-3’-β-quinovosyl zeaxanthin, a novel carotenoid glycoside synthesized by Escherichia coli

cells expressing the Pantoea ananatis carotenoid biosynthesis gene cluster. Appl. Microbiol. Biotechnol., 97 (19): 8479-8486.

127. Harada, H., Maoka, T., Osawa, A., Hattan, J., Kanamoto, H., Shindo, K., Otomatsu, T., and Misawa, N. (2014) Construction of transplastomic lettuce (Lactuca sativa) dominantly producing astaxanthin fatty acid esters and detailed chemical analysis of generated carotenoids. Transgenic Res. 23 (2): 303-315.

128. Takemura, M., Maoka, T., and Misawa, N. (2014) Carotenoid analysis of a liverwort Marchantia polymorpha and functional identification of its lycopene β- and ε-cyclase genes. Plant Cell. Physiol., 55 (1): 194-200.

129. Makino, T., Katsuyama, Y., Otomatsu, T., Misawa, N., and Ohnishi, Y. (2014) Regio- and stereospecific hydroxylation of various steroids at the 16α position of the D ring by the Streptomyces griseus cytochrome P450 CYP154C3. Appl. Environ. Microbiol., 80 (4): 1371-1379.

＜和文総合論文 (査読付き)＞ 1． 新藤一敏，三沢典彦，Biphenyl 分解経路系酵素遺伝子群を複合的に利用した効率的微生物変換技

術(CellCombiChem)の開発． 有機合成化学協会誌，64，109-116, 2006． ＜総説＞ 1．池永裕, 三沢典彦, カロチノイド生合成経路の解明. バイオサイエンスとインダストリー, 49 (12):

1274-1277, 1991 (12 月 1 日). 2．三沢典彦, 遺伝子レベルでのカロテノイド生合成経路の解明と代謝工学. ファルマシア, 31 (4):

369-372, 1995 (4 月 1 日). 3．三沢典彦, 天然物化学的・分子生物学的・代謝工学的手法によるカロチノイド生合成研究. 日本農芸

化学会誌, 69 (12): 1567-1572, 1995 (12 月 1 日). 4．三沢典彦, 有用カロテノイド生産のための代謝工学. バイオサイエンスとインダストリー, 53:

1036-1041, 1995.

5．三沢典彦, 海洋細菌のカロテノイド生合成遺伝子 －色素の多様性と遺伝子の単純性のアウフヘーベ

ン. マリンバイオテクノロジー研究会報, 8 (3): 24-31, 1995. 6．三沢典彦, 遺伝子レベルで解明されたカロテノイド生合成経路. 蛋白質 核酸 酵素, 41 (4): 337-346,

1996 (4 月 1 日). 7．三沢典彦, カロテノイドの生理活性とバイオテクノロジー. FFIジャーナル, 169: 90-94, 1996 (7月 1日). 8．三沢典彦, カロテノイド生合成阻害剤抵抗性植物の作出. 植物の化学調節, 31 (2): 143-149, 1996 (12 月

25 日). 9．三沢典彦, セミナー室 メタボリックエンジニアリングの展開−2. 大腸菌・酵母によるカロテノイド

生産. 化学と生物, 35: 60-68, 1997 (1 月). 10．三沢典彦, トピックス イソペンテニルピロリン酸の異性化反応は細菌のイソプレノイド生合成のキ

ー段階である？ 生物工学会誌, 75 (5): 385, 1997 (9 月 25 日). 11．Misawa, N., and Shimada, S., Metabolic engineering for the production of carotenoids in non-carotenogenic

10

bacteria and yeasts. J. Biotechnol., 59: 169-181, 1998. 12．三沢典彦, カロテノイド生産のための食用酵母 Candida utilis の代謝工学. ケミカルエンジニヤリン

グ, 44: 447-452, 1999 (6 月). 13. 三沢典彦, 古川謙介, 新藤一敏, ジオキシゲナーゼによる芳香族化合物のバイオコンバージョン. バ

イオサイエンスとインダストリー, 63: 13-19, 2005 (11 月). 14. 三沢典彦, カロテノイド生合成遺伝子とバイオテクノロジー. FFI ジャーナル, 212: 532-538, 2007 (7 月). 15. 三沢典彦, β-カロテンを強化した遺伝子組換え作物. バイオミディア, 生物工学会誌, 86: 629, 2008. 16. Misawa, N., Pathway engineering of plants toward astaxanthin production. Plant Biotechnol., 26: 93-99, 2009

(March). 17. 藤澤雅樹, 三沢典彦, ナタネ等の作物におけるカロテノイド生産制御技術の開発. バイオインダスト

リー, 5: 28-35, 2009 (5 月). 18. 三沢典彦, 遺伝子組換え植物によるカロテノイドの生産. オレオサイエンス, 9 (9): 385-391, 2009 (9 月). 19. Harada, H. and Misawa, N., Novel approaches and achievements in biosynthesis of functional isoprenoids in

Escherichia coli. Appl. Microbiol. Biotechnol., 84: 1021-1031, 2009.

20. Misawa, N., Carotenoid β-ring hydroxylase and ketolase from marine bacteria －Promiscuous enzymes for

synthesizing functional xanthophylls. Mar. Drugs, 9 (5): 757-771, 2011.

21. 三沢典彦, 音松俊彦, 基質特異性の広い融合型シトクロムP450を用いたバイオコンバージョン. バイ

オサイエンスとインダストリー, 69: 299-302, 2011. 22. Misawa, N., Pathway engineering for functional isoprenoids. Curr. Opin. Biotechnol., 22: 627-633, 2011. 23. 原田尚志, 三沢典彦, 大腸菌を用いた植物由来テルペン生合成酵素遺伝子の効率的機能解析システム.

化学と生物, 49 (12): 825-833, 2011. 24. 三沢典彦, シリーズ くらしの最前線 85・日本の遺伝子組換え農作物・食品の現状. 家政学会誌, 63 (3):

151-155, 2012. 25. 三沢典彦, 植物におけるルテインの生合成とその役割, 吉川敏一 企画, Functional Food, 6 (1): 8-13,

2012 (7 月). 26. 八反順一郎, 三沢典彦, 芳香植物のテルペン合成酵素遺伝子のカタログ化－ツバキの花を例に－,

Aroma Research (Journal of Aroma Science Technology and Safety) , No. 57 (15 (1)): 38-47, 2014 (2 月 28 日). 27. Shindo, K. and Misawa, N., New and rare carotenoids isolated from marine bacteria and their antioxidant

activities. Mar. Drugs, 12: 1690-1698, 2014. ＜著書＞ 1. 石田功, 三沢典彦 著, "細胞工学実験操作入門", 講談社サイエンティフィク, 1992 (10 月 10 日). 2. 三沢典彦, 石田功, 大腸菌による大量発現. 石田功, 安藤民衞 編, "遺伝子発現実験マニュアル",

pp.70-99, 講談社サイエンティフィク, 1994 (12 月 10 日)． 3. 三沢典彦, β-カロテン, リコぺン生産. 籏野昌弘 監修, "新タンパク質応用工学", pp.598-604, フジ・テ

クノシステム, 1996. 4. Sandmann, G., Misawa, N., and Böger, P., Steps towards genetic engineering of crops resistant against

bleaching herbicides. In "Herbicide Resistant Crops". ed. by Duke, S. O., pp. 189-200, Lewis Publ., Boca Raton, FL, 1996.

5. Misawa, N., Carotenoid biosynthesis at the gene level. In "Dynamic Aspects of Natural Products Chemistry

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-Molecular Biological Approaches-", ed by Ogura, K. and Sankawa, U., PP.49-70, Kodansya, Tokyo, 1997. 6. 三沢典彦，植物細胞による生産 ＜農学，薬学への応用＞. 松橋通生, 真野佳博 編, "ポイントがわか

る分子生物学", pp.112-113, 丸善, 1999 (2 月 20 日). 7. 平井愛山, 龍野一郎, 三沢典彦, 新藤一敏, 紅参サポニン(ジンセノサイド Rg3) ヒト血中濃度測定の

試み. 熊谷 朗 監修, "薬用人参 2000", pp.54-57, 共立出版, 2000. 8. Sandmann, G. and Misawa, N. Fungal Carotenoids. In "the Mycota X，Industrial Applications", ed. by

Osiewacz, H. D., pp. 247-262, Springer-Verlag Berlin Heidelberg, 2002. 9. 三沢典彦, 第 5章 植物代謝工学の工業生産への応用 第 7節 二次代謝産物 第 2項 カロテノイド. 新

名惇彦, 吉田和哉 監修, ”植物代謝工学ハンドブック”, pp. 614-626, エヌティーエス, 2002 (6 月 25 日). 10. 三沢典彦, 代謝工学. 鈴木昭憲, 荒井綜一 編, "農芸化学の事典", pp. 521-523, 朝倉書店, 2003 (10 月 20

日). 11. 三沢典彦, 原田尚志, 内海龍太郎, 9 大腸菌を宿主としたパスウェイエンジニアリングによる食品成

分イソプレノイド (カロテノイド，セスキテルペン) の生産. 植田充美 監修, "微生物によるものづく

り －化学法に代わるホワイトバイオテクノロジーの全て－", pp. 107-117, シーエムシー出版, 2008 (6月 20 日).

12. 三沢典彦, 音松俊彦, 4.4 バイオコンバージョンによる多様化技術. "天然物化学 －海洋生物編－". 山村庄亮, 長谷川宏司, 木越英夫 編, pp. 406-416, アイピーシー, 2008 (6 月 30 日).

13. 三沢典彦, II 発酵と微生物 2.5 食品関連分野 (5) カロテノイド色素, pp. 136-137; 3.4 代謝工学 (3) カロテノイド, pp. 163-166; (4) セルコンビケム, pp. 166-168, 渡邉 信, 西村和子, 内山裕夫, 奥田 徹, 加来久敏, 広木幹也 編, "微生物の事典"，朝倉書店，2008 (9 月 25 日).

14. 三沢典彦, 第 10 章 海洋遺伝子資源からの P450 遺伝子の単離と解析. 松永 是, 竹山春子 監修, "マリ

ンメタゲノムの有効利用", pp. 123-140, シーエムシー出版, 2009 (7 月 24 日). 15. 梅野太輔, 古林真衣子, 三沢典彦, 第３章 カロテノイドの生合成, 宮下和夫 監修, “カロテノイドの

科学と最新応用技術”, pp. 27-37, シーエムシー出版, 2009 (9 月 30 日). 16. Misawa, N., Carotenoids, In Comprehensive Natural Products II Chemistry and Biology; Mander, L., Lui,

H.-W., Eds.; Elsevier: Oxford, 2010; volume 1, pp. 733–753. 17. Fujisawa, M. and Misawa, N., Enrichment of carotenoids in flaxseed by introducing a bacterial phytoene

synthase gene. In “Plant Secondary Metabolism Engineering, Methods and Applications”, ed. by Fett-Neto,

A.G., Methods in Molecular Biology 643, Humana Press, pp. 201-211, 2010. 18. Harada, H. and Misawa, N., Novel approach in the biosynthesis of functional carotenoids in Escherichia coli.

In “Microbial Carotenoids from Bacteria and Microalgae, Methods and Protocols”, ed. by Barredo, J.L., Methods in Molecular Biology 892, Humana Press, pp.133-141, 2012.

19. 三沢典彦, 新進化論とアスタキサンチンを産生する高等植物, 吉川敏一, 内藤裕二 監修, “アスタキ

サンチンの機能と応用”, pp. 29-36, シーエムシー出版, 2012 (7 月). 20. Hattan, J. and Misawa, N., Production of functional isoprenoids through pathway engineering, ed. by Grunwald,

P., In “Industrial Biocatalysis”, Pan Stanford Publishing, Singapore, in press. ＜国際学会、国際シンポ発表；第 1 または最終演者の場合のみ＞ 1. Misawa, N., Yamano, Y., Ohyama, K., and Komano, T. Protoplast culture of Euphorbia tirucalli and oil body

formation, Proc. 5th Intl. Cong. Plant Tissue & Cell Culture, pp. 607-608, Shizuoka, Japan, 1982.

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2. Misawa, N., A single gene for multiple steps in carotenoid biosynthesis. (Invited Speaker). In “Gordon Research Conference on Chemistry and Biology of Carotenoids”, Casa Sirena Resort, Oxnard, California, USA, March 8-13, 1992.

3. Misawa, N. and Sandmann, G., Functional expression of the Erwinia uredovora carotenoid biosynthesis gene crtI in transgenic plants. In “10th International Symposium on Carotenoids”, Trondheim, Norway, 1993.

4. Misawa, N., Elucidation of the carotenoid biosynthetic pathway using the biosynthetic genes. In "4th US-Japan Seminar on the Biosynthesis of Natural Products -From Pathway to Genes to Products-", Fuji Kyoiku Kenshujo, Susono-shi, Shizuoka, Japan, 1994.

5. Misawa, N., Elucidation of astaxanthin biosynthetic pathway at the gene level. In “Pacifichen 95”, Honolulu, HI, USA, 1995.

6. Fraser, P. D. and Misawa, N., The biosynthetic pathway from β-carotene to astaxanthin elucidated at the level of the genes and enzymes. In “11th International Symposium on Carotenoids”, Leiden, the Netherlands, 1996.

7. Misawa, N., Metabolic engineering for the production of carotenoids in Escherichia coli and other microorganisms. In “rDNA Biotechnology IV: Metabolic engineering”, Danvers, Massachusetts, USA, 1996.

8. Misawa, N., Metabolic engineering for production of useful carotenoids in yeasts, Proceedings of the International Symposium on Strain Development for Bioindustry, ed by the Korean Society for Applied Microbiology, pp. 37-44. Seoul, Korea, April 25-26, 1997.

9. Misawa, N., Analysis of isoprenoid metabolism in chloroplasts using transgenic plants. In "5th US-Japan Seminar on the Biosynthesis of Natural Products”, Sun Mountain Lodge, Winthrop, Washington, USA, 1997.

10. Misawa, N., Miura, Y., Shimada, H., Saito, T., Fraser, P. D., and Kondo, K., Production of carotenoids by the food yeast Candida utilis, Society for Industrial Microbiology 50th Anniversary Annual meeting Program & Abstracts, pp. 94, Crystal Gateway Marriott, Arlington VA, USA, 1999.

11. Misawa, N., Eguchi, T., Dekishima, Y., Matsushima, Y., Takagi, M., Kuzuyama, T., Seto, H., Kakinuma, K., New methodology to multiply various highly deuterated carotenoids from deuterated mevalonate using triply engineered Escherichia coli. 13th International Carotenoid Symposium, pp. 166, Renaissance Ilikai Hotel, Honolulu, HI, USA, Jan., 2002.

12. Kasai, Y., Shindo, K., Harayama, S., and Misawa, N., Molecular characterization and substrate specificity of polycyclic aromatic hydrocarbon dioxygenase from Cycloclasticus sp. strain A5. Marine Biotechnology Conference 2003, pp. 84, Makuhari Messe, Chiba, Japan, Sep., 2003.

13. Misawa, N. and Shindo, K., Synthesis of various new aromatics hydroxylated by bacterial cells expressing modified biphenyl-catabolic genes. 10th Congress of Federation of Asian & Oceanian Biochemists and Molecular Biologists (FAOBMB Congress), pp. 146, Bangalore, India, Dec., 2003.

14. Shindo, K., Furukawa, K., and Misawa, N., Synthesis of new picolinic acid compounds by growing cell reactions using Escherichia coli that expresses biphenyl-catabolic genes (bphABC). 2nd International Congress on Biocatalysis, pp. 225-226, Hamburg, Germany, Aug.-Sep., 2004.

15. Peng, X., Maruyama, T., Shindo, K., Kanoh, K., Ikenaga, H., and Misawa, N., Conversion of dimethylnaphthalenes and xylenes to their carboxylic acids by recombinant Escherichia coli expressing oxygennase and dehydrogenase genes. 2nd International Congress on Biocatalysis, pp. 227-228, Hamburg, Germany, Aug.-Sep., 2004.

13

16. Choi, S. -K. and Misawa, N., Characterization of bacterial β-carotene ketolases and β-carotene hydroxylases needed for astaxanthin production. 14th International Symposium on Carotenoids. Edinburgh, UK, July, 2005 (Carotenoid Sci., 9, 34, 2005).

17. Nishida, Y., Adachi, K., Kasai, H., Shizuri, Y., Shindo, K., Sawabe, A., Komemushi, S., Miki, W., and Misawa,

N., Isolation of a carotenoid biosynthesis gene cluster encoding a novel enzyme, 2,2’-β-hydroxylase, from Brevundimonas sp. SD212 and combinatorial biosynthesis of new or rare xanthophylls. The 14th International Symposium on Carotenoids. Edinburgh, UK, July, 2005 (Carotenoid Sci., 9, 85, 2005).

18. Misawa, N., Isolation of new P450 CYP153 genes and terminal hydroxylations of alkanes and alkyl aromatic compounds. Pacific Rim Summit of Industrial Biotechnology and Bioenergy, pp. 38-39, Honolulu, Hawaii, USA, January, 2006.

19. Misawa, N., Pathway engineering of oil crops for carotenoid production. The 15th International Symposium on Carotenoids, Okinawa, Japan, June, 2008 (Carotenoid Sci., 12, 65, 2008).

20. Misawa, N., Carotenoid biofortification of oil crops, rape and flax plants. The 1st International Conference on Health and Longevity Sciences. Yaku-shoku Dogen, ‘Medicine and Food, Sharing a Common Origin’ , pp. 21-22, University of Shizuoka, Japan, December 18-19, 2008.

21. Harada, H., Fujisawa, M., Kuzuyama, T., and Misawa, N., Efficient production of useful isoprenoids through early isoprenoid pathway-engineered Escherichia coli. TERPNET 2009 (9th International Meeting: Biosynthesis and Function of Isoprenoids in Plants, Microorganisms and Parasites), pp. O-30, The University of Tokyo, Japan, May 25-29, 2009.

22. Fujisawa, M., Takita, E., Harada, H., Watanabe, M., Choi, S. -K., Sakurai, N., Suzuki, H., Ohyama, K., Shibata, D., and Misawa, N., Pathway engineering of carotenoid biosynthesis in oilseed crops. TERPNET 2009, pp. P-88, The University of Tokyo, Japan, May 25-29, 2009.

23. Shindo, K. and Misawa, N., Novel C30 carotenoids from marine bacteria. TERPNET 2009, pp. P-90, The University of Tokyo, Japan, May 25-29, 2009.

24. Misawa, N., Functional analysis in Escherichia coli of biosynthesis genes for isoprenoids, carotenoids and sesquiterpenes, and their production with higher plants. 2010 International Meeting of the Microbiological Society of Korea, pp. 115-117, Uljin-Gun, Gyeongsangbuk-Do, Korea, May 6-7, 2010.

25. Misawa, N., Pathway engineering for efficient production of astaxanthin in lettuce plants. The 16th International Symposium on Carotenoids, Krakow, Poland, July 17-22, 2011 (Acta Biologica Cracoviensia, 53 (suppl. 1): 83-84, 2011).

26. Hattan, J. and Misawa, N., Novel sesquiterpene synthase genes from edible plants for efficient production of β-caryophyllene and β-eudesmol in Escherichia coli. International Conference and Exhibition on Nutraceuticals and Functional Foods 2011, pp. 164, Sapporo, Japan, November 14-17, 2011.

＜特許出願＞ 1．三沢典彦，小林和男，中村克己，カロチノイドの合成に有用な DNA 鎖，特願平 2-53255，1990 年 3

月 5 日［優先件主張：特願平 1-103078（1989 年 4 月 21 日）］，麒麟麦酒(株) 出願．日本（特許第 2950888号），米国，ヨーロッパで特許成立．

2．三沢典彦，山野重幸，ブリーチング除草剤耐性植物の製造法，特願平 5-163926，1993 年 6 月 8 日, 麒麟麦酒(株) 出願．日本で特許成立（特許第 3759628 号）．

14

3．三沢典彦，近藤恵二，梶原 将，横山昭裕，キサントフィルの合成に有用な DNA およびキサントフ

ィルの製造法，PCT/JP94/02220, 1994 年 12 月 26 日［優先権主張：特願平 5-348737（1993 年 12 月 27日）及び 特願平 6-235917（1994 年 9 月 5 日）］，麒麟麦酒(株) , (株)海洋バイオテクノロジー研究所 出願．日本（特許第 3375639 号），米国，ヨーロッパ，オーストラリア，韓国で特許成立．

4．梶原 将，三沢典彦，近藤恵二，ケト基導入酵素，それをコードする DNA およびケトカロチノイド

の製造法，特願平 7-47266，1995 年 3 月 7 日, 麒麟麦酒(株) 出願．日本（特許第 2960967 号），米国，

ヨーロッパで特許成立． 5．梶原 将，三沢典彦，近藤恵二，カロチノイド生産量の増量に有用な DNA 鎖，特願平 7-51234，1995

年 3 月 10 日, 麒麟麦酒(株) 出願．日本（特許第 3151371 号，特許第 3403381 号），ヨーロッパ（European patent No. 1760150），米国，オーストラリア，イスラエルで特許成立．

6．西野輔翼，里見佳子，三沢典彦，植物性成分の動物での産生法、ストレス耐性動物の作製法および

植物性成分の用途，特願平 7-210022，1995 年 7 月 26 日, 麒麟麦酒(株) 出願． 7．近藤恵二，梶原 将，三沢典彦，キャンディダ・ユティリス酵母の形質転換系、およびそれによる異

種遺伝子の発現，PCT/JP95/01005, 1995 年 5 月 25 日（特願平 7-529524）, 麒麟麦酒(株) 出願．日本（特

許第 3385027 号，特許第 4017966 号），米国，ヨーロッパで特許成立． 8．Paul D. Fraser，三沢典彦，酵素法による有用カロテノイドの製造法，特願平 8-168857，1996 年 6 月

28 日, 麒麟麦酒(株) 出願． 9．三沢典彦，島田裕士，三浦 裕，近藤恵二，カロチノイド生産の増量に有用な遺伝子、およびカロチ

ノイドの製造法，特願平 9-58012，1997 年 3 月 12 日, 麒麟麦酒(株) 出願． 10．三沢典彦，横山昭裕，カロテノイド配糖体およびその製造法，特願平 9-140460，1997 年 5 月 29 日, 麒

麟麦酒(株), (株)海洋バイオテクノロジー研究所 出願． 11．三沢典彦，正元和盛，金子貴一，藤 博幸，β-カロチンハイドロキシラーゼ遺伝子およびその使用，

特願平 9-213648，1997 年 8 月 7 日, 麒麟麦酒(株) 出願．日本で特許成立（特許第 3874897 号）． 12．三沢典彦，新藤一敏，岡崎 寛，古川謙介，堀之内末治，水酸化された復素環化合物および芳香族カ

ルボン酸の製造法および改変された芳香環ジオキシゲナーゼ，特願 2001-270878，2001 年 9 月 6 日［優

先件主張：特願 2000-279703（平成 12 年 9 月 14 日）］, 麒麟麦酒(株) 出願［→ 神戸天然物化学(株)］．N. Misawa et al, Method for producing hydroxylated heterocyclic compounds and aromatic carboxylic acids, and modified aromatic ring dioxygenases. 米国出願．日本で特許成立（特許第 4972255 号）．

13．寺本真紀，三沢典彦，単環式カロテノイドの合成に有用な遺伝子、および単環式カロテノイドの製

造法，特願 2002-323307，平成 14 年 11 月 7 日, (株)海洋バイオテクノロジー研究所 出願［→ キリン

ホールディングス(株)］．日本で特許成立（特許第 4205398 号）． 14．三沢典彦，加々美 修，新藤一敏，カルボン酸類の製造方法，特願 2003-290804，平成 15 年 8 月 8 日,

(株)海洋バイオテクノロジー研究所 出願［→ 神戸天然物化学(株)］． 15．笠井由紀，三沢典彦，新藤一敏，新規な芳香環ジオキシゲナーゼ遺伝子群及びその用途，PCT

/JP03/15102（特願 2004-570723），平成 15 年 11 月 26 日［優先権主張：特願 2002-347240（平成 14 年

11 月 29 日）及び 特願 2003-324117（平成 15 年 9 月 17 日）］, (株)海洋バイオテクノロジー研究所 出願［→ 神戸天然物化学(株)］．日本で特許成立（特許第 4390713 号）．

16．三沢典彦，新藤一敏，芳香族ジオールの製造方法，PCT/JP2004/002654，平成 16 年 3 月 3 日［優先権

主張：特願 2003-57867（平成 15 年 3 月 4 日）］，(株)海洋バイオテクノロジー研究所 出願［→ 神戸天

然物化学(株)］．

15

17. 丸山高廣，石倉正治，瀧 寛則，三沢典彦，新規ペプチド、アルキル基が水酸化された芳香族化合物

の製造法及び環境の浄化方法，特願 2004-235918，平成 16 年 8 月 13 日［優先権主張：特願 2004-60036（平成 16 年 3 月 4 日）］， (株)海洋バイオテクノロジー研究所 出願［→ 神戸天然物化学(株)］．

日本で特許成立（特許第 4643947 号）． 18．西田康宏，足立恭子，笠井宏朗，志津里芳一，米虫節夫，三沢典彦，新規なカロテノイドヒドロキ

シラーゼ遺伝子及び水酸化されたカロテノイドの製造法及び新規なゲラニルゲラニルピロリン酸シ

ンターゼ遺伝子，PCT/JP2004/16297，平成 16 年 11 月 4 日，(株)海洋バイオテクノロジー研究所 出願

［→ キリンホールディングス(株)］．米国で特許成立（US 特許番号 7,695,931），ヨーロッパ（スイス，

ドイツ，フランス，イギリス）で特許成立（欧州特許番号 1693377），日本で特許成立（特許第 4398431号，特許第 4460621 号）．

19. 新藤一敏，加々美 修，三沢 典彦，ピコリン酸類の製造方法, PCT/JP2005/2755，平成 17 年 2 月 22 日, 特願 2006-510638［優先権主張：特願 2004-61238（平成 16 年 3 月 4 日）］(株)海洋バイオテクノロジ

ー研究所 出願［→ 神戸天然物化学(株)］．日本で特許成立（特許第 4744437 号）． 20. 彭 学，三沢典彦, 新規な芳香族アルコール酸化酵素遺伝子及び芳香族カルボン酸の製造法，特願

2005-174511, 平成17年6月15日，(株)海洋バイオテクノロジー研究所 出願［→ 神戸天然物化学(株)］．日本で特許成立（特許第 4704814 号）．

21. 彭 学，瀧 寛則，加納周雄，三沢典彦，新規な芳香族デヒドロゲナーゼ遺伝子及び芳香族カルボン酸

の製造法，PCT/2005/004163，平成 17 年 3 月 3 日 , 特願 2006-531252［優先権主張：特願 2004-235414（平成 16 年 8 月 12 日）］(株)海洋バイオテクノロジー研究所出願［→ 神戸天然物化学(株)］．日本で

特許成立（特許第 4881160 号）． 22. 野舘美保，三沢典彦，井手野 晃，小川 順，清水 昌，シトクロム P450 モノオキシゲナーゼの生産方

法，特願 2005-038576，平成 17 年 2 月 16 日 , (株)海洋バイオテクノロジー研究所 出願［→ 神戸天然

物化学(株)］．日本で特許成立（特許第 4750430 号）． 23. 野舘美保，三沢典彦，融合型シトクロム P450 モノオキシゲナーゼ，特願 2005-083122，平成 17 年 3

月 23 日 , (株)海洋バイオテクノロジー研究所 出願［→ 神戸天然物化学(株)］． 24. 久保田光俊，野舘美保，内山 拓，三沢典彦，新規 P450 遺伝子及びその単離法及びそれを用いた酸化

化合物の生産法，特願 2005-083019，平成 17 年 3 月 23 日 ,［優先権主張：特願 2004-326139（平成 16年 11 月 10 日）］, (株)海洋バイオテクノロジー研究所 出願［→ 神戸天然物化学(株)］．

25. 加々美 修，三沢典彦，新藤一敏，改変型芳香環ジオキシゲナーゼ及び水酸化フラボン類化合物の製

造法，特願 2005-313177，平成 17 年 10 月 27 日，(株)海洋バイオテクノロジー研究所 出願［→ 神戸

天然物化学(株)］．日本で特許成立（特許第 4800000 号）． 26. 久保田光俊，野舘美保，内山 拓，三沢典彦，P450 遺伝子の単離法，PCT/JP2005/20180，平成 17 年 11

月 2 日 , (株)海洋バイオテクノロジー研究所 出願［→ 神戸天然物化学(株)］． 27．崔 善江，三沢典彦，カロテノイドケトラーゼ及びカロテノイドヒドロキシラーゼ遺伝子を利用した

アスタキサンチンまたはその代謝物の製造法，PCT/JP2005/9609（US No. 11/597,362），平成 17 年 5 月

26 日［基礎出願：特願 2004-166625（平成 16 年 6 月 4 日）］，(株)海洋バイオテクノロジー研究所 出願［→ キリンホールディングス(株)］．米国で特許成立（US 特許番号 7,999,151），ヨーロッパ（スイ

ス，ドイツ，スペイン，スウェーデン）で特許成立（欧州特許番号 1780281），日本で特許成立（特

許第 4803739 号）． 28. 蓮沼誠久，三宅親弘，三沢典彦，アスタキサンチンの製造方法．特願 2007-196776，平成 19 年 7 月

16

27 日, (財)地球環境産業技術研究機構 出願． 29. 蓮沼誠久，三宅親弘，三沢典彦，新藤一敏，新規カロテノイド化合物．特願 2008-017443，平成 20

年 1 月 29 日, (財)地球環境産業技術研究機構 出願． 30. 原田尚志，三沢典彦，内海龍太郎，組換え大腸菌及びそれを用いたイソプレノイドの製造方法．特願

2008-51539，平成 20 年 3 月 3 日, (株)海洋バイオテクノロジー研究所, 近畿大学 出願［→ キリンホ

ールディングス(株) , 近畿大学］，日本で特許成立（特許第 5405030 号）． 31. 音松俊彦, 三沢典彦, 藤田尚也, CYP153 による芳香族化合物の製造方法, 特願 2008-131734, 平成 20

年 5 月 20 日［基礎出願：特願 2007-139575（平成 19 年 5 月 25 日）］, 神戸天然物化学(株) 出願． 32. 牧野拓也，三沢典彦，新藤一敏，音松俊彦，CYP110 による芳香族化合物の製造方法, 特願 2009-45213,

平成 21年 2月 27日, 神戸天然物化学(株) 出願． 33. 三沢典彦, 新藤一敏, P450 による難化学合成化合物の製造方法及び新規セスキテルペン, 特願

2009-137211, 平成 21 年 6 月 8 日, キリンホールディングス(株) 出願．

34. 内海龍太郎, 余 豊年, 岡本 尚, 原田尚志, 三沢典彦, セスキテルペン変換酵素遺伝子及びそれを利

用した酸化セスキテルペンの製造方法, 特願 2009-205961，平成 21 年 9 月 7 日，近畿大学, キリンホ

ールディングス(株) 出願．

35. 藤澤雅樹, 原田尚志, 三沢典彦, 兼目裕充, 新規セスキテルペン合成酵素遺伝子及びそれを利用したセス

キテルペンの製造方法, 特願2009-287139, 平成21年12月18日, キリンホールディングス(株) 出願．

日本で特許成立（特許第 05457159 号）． 36. 杉村 哲, 梅基直行, 三沢典彦, 原田尚志, 矢崎一史, 新規のテルペン合成酵素活性を有するタンパク質

とそれをコードする遺伝子, 特願 2009-295889, 平成 21 年 12 月 25 日, キリンホールディングス(株) 出願．

37. 牧野拓也，三沢典彦，音松俊彦，16α位水酸化ステロイド化合物の製造方法，特願 2011-36447，平成

23 年 2 月 22 日，神戸天然物化学(株) 出願． 38. 梅野太輔, 古林真衣子, 三沢典彦, 高市真一, 炭素数50のカロテノイドの製造方法, PCT/JP2012/64817,

平成 24 年 6 月 8 日［基礎出願：特願 2011-130326 (平成 23 年 6 月 10 日)］， 国立大学法人 千葉大

学 出願． 39. 八反順一郎, 三沢典彦, セスキテルペン合成酵素遺伝子及び該遺伝子を利用したセスキテルペンの製造

方法, 特願 2012-186804, 平成 24 年 8 月 27 日［優先権主張：特願 2011-185356 (平成 23 年 8 月 27 日)］，石川県公立大学法人 石川県立大学 出願．

40. 三沢典彦, 原田尚志, 眞岡孝至, 植物からのカロテノイドの抽出方法, 特願 2012-198865, 平成 24 年 9月 10 日, 神戸天然物化学(株) 出願．

41. 三沢典彦, 八反順一郎, 新藤一敏, 新規イソフラボン配糖体、及び該イソフラボン配糖体を含むアン

ドロゲン受容体結合組成物又はアンドロゲン受容体アンタゴニスト組成物, 特願 2012-272894, 平成

24 年 12 月 13 日, 石川県公立大学法人 石川県立大学 出願．

以上

17