hematocrit, HctHt,HCT packed cell volume, PCV

layer after blood centrifugated

plasm

platlet white cellreduced hemoglobin oxygen red cell

Red Blood Cells

1Wintrobe2.microhematocrit

3 Hct = RBCMCV

1 ICSHWintrobe WHO 2

1. 2. Hct

Wintrobe0.40.540.370.47 0.470.040.420.05 132

mean corpuscular volume, MCVfl(mean corpuscular hemoglobin, MCH)pg(mean corpuscular Hemoglobin concentration, MCHC)g/L

1 1 Hct MCV= = fl RBC 2 Hb MCH= = pg RBC 3 Hb MCHC= = Hct

2 MCVRBCHbMCHMCHCMCH=Hb/RBCMCHC=Hb/RBCMCV

1.MCV MCV360flMCV600mg/LMCVCV1%CV10%2.MCH MCH3.MCHC MCHCHctHb

1. 2. 333.XBMCHC33%,2%3%60

MCV 8092flMCH 2731pg MCHC320360g/L MCV 80100flMCH 2734pgMCHC320360g/L

(red blood cell volume distribution width, RDW)CV

RDWMCV RDWRDW 11.6%14.6%

1 1983BessmanMCVRDW 2.iron deficiency anemia, IDA RDW95% 3. RDW-RDW

reticulocyte, RetRETribomucleic acidRNAbrilliant cresyl blue79mHeilmyer5ICSH4Heilmyer 0

1 2 -YRNAflow cytometryFCM 3

1

reticulocyte

Miller

2 high fluorescent ratioHFRmiddle fluorescent rationMFRlow fluorescent ratioLFR

3 RetRctRct%mean reticulocyte volume, MCVrreticulocyte hemoglobin distribution width , HDWrreticulocyte hemoglobin concentration ,HCRmean reticulocyte hemoglobin concentration , MCHCrLFRMFRHFRreticulocyte maturity index RMIRMI=MFR+HFR/LFR100

4 12=/L

3reticulocyte production index, RPI2d Hct 1 RPI= Ret% Hct 2Hct45%40%

1 Miller2 10 00050 000Howell-Jolly 1 0.0080.022575109/L0.020.062

0.20.3IV0.70.8III

1 1Ret 2

2 Ret3

RNA

basiphilic stippling count1000

50%= 510 310-4

1. 2.

erythrocyte sedimentation rateESR310min40min

1Westergren 1hmm

2 National Clinical Committee of Laboratory Standardization NCCLS2000EDTA-K21HtH-t2201000

1 ICSH

2

1

2 3 45

50020mm/h030mm/h>85030mm/h042mm/h010mm/h

1132 123d 2

345 62

white blood cell WBC leukocyteLEUgranulocyteGRANlymphocyteLmonocyteM

1 2

1 1/5001 2

WBCWBC

WBC/HP WBC109/L

24 47 46 79 610 1012 1012 ( 13l8 )

2.(routine checking standard ,RCS) 3. V Xi Xm

4. 22 rx1x2125. (CV) n x1x2

(4l0) 109/L (1520) 109/L62 (1112) 109/L

(differential countDC) 1

2

1. 1 2 2. 10020095%

3. 4. National Clinical Committee of Laboratory StandardizationNCCLSEDTAK3 Romanowaky2004200(800)

% 109/L

15 (0.040.5) 5070 (27) 0.55 (0.020.5) 01 (01) 2040 (0.84) 38 (0.120.8)

(neutrophilN) (mitotic pool)(maturation pool)(storage pool)(circulating pool)marginating pool

granulocyte pool

10 Bone marrow 10h 1~2Mitotic poolMaturation poolStorage poolMargin poolCirculating pool

(1)(neutrophilia)70%7l09/L1) 69d4530%

2)

(1020) l09/L(2030) l09/L

()1236h ,10l09/L12d1

12h(1020) l09/L

MICMM

(acute myelogenous leukemiaAML)(acute lymphocytic leukemiaALL)(1050) l09/L100l09/L

10%(100600) l09/L 50l09/L

100l09/L10%

2neutropenia 1.5l09/Lgranulocytopenia0.5l09/L(agranulocytosis)

12(aleukemic leukemia)

34systemic lupus erythematosusSLE5

3

1)shift to the left5%

(5%)10%25%

2)shift to the right53%

2. 3. (basophilB) 1basophilia0.05109/L1IgE2 3 2(basopenia)

4. 1(lymphocytosis) 4109/L 7.2109/L4 9109/L 1

2

2(lymphopenia)

5. 1(monocytosis)0.8109/L 12 2 2

(eosinophilE )

1 2

(0.050.5)109/L 1

2(eosinophilia) 0.5109/L (1)10% (2) (3)

(4) (5) (6)

3(eosinopenia)

4 (1) (2) (3)adrenocorticotropic hormoneACTHACTH 25mg 0.1%0.5mlACTH

50%50%80%l00%50%(Addison)4%

1 Romanovsky 2

1. 4

/

2.11 Dhle

2

3(Auer body)

4PelgerHuet2()

ChediakHigashi1025m

Alder-Reilly

MayHegglinDhle

21abnormal lymphocyteTDowney3I()() ()

23

3plasma cell

1MottMott cell

2flame cellIgA

3Russell (Russell body) 23m