HPLCHAMILTON HPLC Polymer Column

HAMILTON HPLC Polymer Column

HAMILTON

http://www.hamiltoncompany.com/HPLC/

PRP `` pH pH 1 13

``

`` 35 MPa

PRP

1.0 100 mm 33 305 mm

HAMILTON Polymer Column2

pH

PRP-1 PS-DVB 100 1 13pH = 1 13, 5 85 , 35 MPa HPLC

PRP-3 PS-DVB 300 1 13 300 HPLC

PRP-C18 PS-DVB C18 100 1 13

C18HPLCLC/MS

PRP-h5 5 PS-DVB 300 1 13 300 HPLC

PRP-X100 PS-DVB 0.19 meq/g 100 1 13

PRP-X110PRP-X110S PS-DVB

0.11 meq/g 100 1 13

PRP-X110S

PRP-X500 Polymer 1.6 meq/gSuperficially

porous 1 13 DNA

PRP-X600 Polymer 1.6 meq/gSuperficially

porous 1 13 DNA

RCX-10 PS-DVB 0.35 meq/g 100 1 13DP8

RCX-30 PS-DVB 1.0 meq/g 100 1 13

PRP-X200 PS-DVB 35 eq/g 100 1 13 1 2

PRP-X400 PS-DVB 2.5 meq/g N/A 1 13

PRP-X800 PS-DVB 1.6 meq/g N/A 1 13 1 2

PRP-X300 PS-DVB 0.17 meq/g 100 1 13

HC-75 PS-DVB

H+

Pb2+Ca2+

5 meq/gm N/A

Pb2+ Ca2+

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PRP-1

PRP-3

PRP-h5

P/N Cat. No. 150 x 2.1 mm I.D. 5 m 79366 7845-79366 86,000

150 x 4.1 mm I.D. 5 m 79444 5055-16000 91,000

250 x 4.1 mm I.D. 5 m 79820 7845-79820 113,000

150 x 4.6 mm I.D. PEEK 5 m 79423 7845-79423 95,000

250 x 4.6 mm I.D. PEEK 5 m 79571 7845-79571 117,000

250 x 4.1 mm I.D. 7 m 79422 7845-79422 108,000

250 x 4.6 mm I.D. PEEK 7 m 79380 7845-79380 117,000

250 x 21.2 mm I.D. 7 m 79352 7845-79352 764,000

150 x 4.1 mm I.D. 10 m 79425 5055-16005 74,000

250 x 4.1 mm I.D. 10 m 79427 5055-16010 93,000

150 x 4.6 mm I.D. PEEK 10 m 79351 7845-79351 78,000

250 x 4.6 mm I.D. PEEK 10 m 79381 7845-79381 97,000

250 x 10 mm I.D. 10 m 79499 7845-79499 188,000

250 x 21.2 mm I.D. 10 m 79478 7845-79478 573,000

250 x 21.2 mm I.D. 12-20 m 79428 5055-16020 454,000

500 x 212 mm I.D. 12-20 m 79350 7845-79350 656,000

250 x 30 mm I.D. 12-20 m 79229 7845-79229 566,000

250 x 50.8 mm I.D. 12-20 m 79493 7845-79493 1,294,000

P/N Cat. No.

10 m 79447 5055-16030 34,000

PEEK 10 m 79317 7845-79317 44,000

12-20 m 79121 7845-79121 33,000

10 m 79445 5055-16035 37,000

PEEK 10 m 79318 7845-79318 39,000

12-20 m 79122 7845-79122 22,000

P/N Cat. No.

150 x 2.1 mm I.D. 10 m 79392 7845-79392 93,000

150 x 2.1 mm I.D. PEEK 10 m 79895 7845-79895 101,000

150 x 4.1 mm I.D. 10 m 79466 5055-16105 98,000

150 x 4.6 mm I.D. PEEK 10 m 79382 7845-79382 101,000

250 x 21.2 mm I.D. 10 m 79147 7845-79147 529,000

250 x 21.2 mm I.D. 12-20 m 79469 5055-16115 498,000

P/N Cat. No.

10 m 79461 5055-16120 35,000

PEEK 10 m 79393 7845-79393 45,000

12-20 m 79123 7845-79123 33,000

10 m 79454 5055-16125 37,000

PEEK 10 m 79395 7845-79395 39,000

12-20 m 79124 7845-79124 22,000

P/N Cat. No.

50 x 2.1 mm I.D. 5 m 79672 7845-79672 76,000

150 x 2.1 mm I.D. 5 m 79673 7845-79673 96,000

250 x 2.1 mm I.D. 5 m 79674 7845-79674 115,000

50 x 4.6 mm I.D. 5 m 79675 7845-79675 76,000

150 x 4.6 mm I.D. 5 m 79676 7845-79676 96,000

250 x 4.6 mm I.D. 5 m 79677 7845-79677 115,000

250 x21.2 mm I.D. 12-20 m 79678 7845-79678 443,000

50 x 2.1 mm I.D. PEEK 5 m 79679 784579679 76,000

150 x 2.1 mm I.D. PEEK 5 m 79680 784579680 96,000

250 x 2.1 mm I.D. PEEK 5 m 79681 784579681 115,000

50 x 4.6 mm I.D. PEEK 5 m 79682 784579682 76,000

150 x 4.6 mm I.D. PEEK 5 m 79683 784579683 96,000

250 x 4.6 mm I.D. PEEK 5 m 79684 7845-79684 115,000

PRP-C18

P/N Cat. No.

5 m 79685 7845-79685 34,000

PEEK 5 m 79687 7845-79687 46,000

12-20 m 79689 7845-79689 37,000

5 m 79686 7845-79686 38,000

PEEK 5 m 79688 7845-79688 40,000

12-20 m 79690 7845-79690 27,000

P/N Cat. No.

100 x 2.1 mm I.D. 5 m 79270 7845-79270 91,000

150 x 2.1 mm I.D. 5 m 79271 7845-79271 101,000

50 x 4.6 mm I.D. 5 m 79261 7845-79261 83,000

150 x 4.6 mm I.D. 5 m 79272 7845-79272 101,000

150 x 10 mm I.D. 5 m 79274 7845-79274 296,000

P/N Cat. No.

10 m 79267 7845-79267 34,000

12-20 m 79277 7845-79277 37,000

10 m 79268 7845-79268 38,000

12-20 m 79278 7845-79278 26,000

HAMILTON Polymer Column4

P/N Cat. No.

250 x 2.1 mm I.D. PEEK 5 m 79190 7845-79190 128,000

150 x 4.6 mm I.D. PEEK 5 m 79174 7845-79174 117,000

250 x 4.6 mm I.D. PEEK 5 m 79181 7845-79181 137,000

100 x 4.1 mm I.D. 10 m 79439 5055-16300 91,000

150 x 4.1 mm I.D. 10 m 79434 5055-16305 98,000

250 x 4.1 mm I.D. 10 m 79433 5055-16310 119,000

150 x 4.6 mm I.D. PEEK 10 m 79354 7845-79354 101,000

250 x 4.6 mm I.D. 10 m 79455 7845-79455 121,000

P/N Cat. No.

10 m 79448 5055-16315 34,000

PEEK 10 m 79383 7845-79383 45,000

12-20 m 79125 7845-79125 33,000

10 m 79446 5055-16320 37,000

PEEK 10 m 79385 7845-79385 39,000

12-20 m 79126 7845-79126 22,000

P/N Cat. No. 150 x 4.1 mm I.D. 7 m 79732 7845-79732 107,000

150 x 4.6 mm I.D. PEEK 7 m 79738 7845-79738 117,000

PRP-X110

P/N Cat. No.

12-20 m 79726 7845-79726 38,000

PEEK 12-20 m 79727 7845-79727 52,000

12-20 m 79728 7845-79728 42,000

PEEK 12-20 m 79729 7845-79729 45,000

P/N Cat. No. 150 x 4.1 mm I.D. 7 m 79733 7845-79733 107,000

250 x 4.6 mm I.D. PEEK 7 m 79741 7845-79741 152,000

PRP-X110S

P/N Cat. No. 150 x 4.6 mm I.D. PEEK 7 m 79573 7845-79573 101,000

PRP-X500

P/N Cat. No. PEEK 12-20 m 79319 7845-79319 70,000

PEEK 12-20 m 79320 7845-79320 39,000

P/N Cat. No. 50 x 4.6 mm I.D. PEEK 7 m 79360 7845-79360 73,000

150 x 2.1 mm I.D. PEEK 7 m 79220 7845-79220 92,000

PRP-X600

P/N Cat. No. PEEK 12-20 m 79361 7845-79361 45,000

PEEK 12-20 m 79362 7845-79362 39,000

P/N Cat. No. 250 x 4.1 mm I.D. 7 m 79465 5055-16505 121,000

150 x 4.1 mm I.D. 7 m 79464 5055-16500 93,000

150 x 4.6 mm I.D. PEEK 7 m 79475 7845-79475 101,000

PRP-X300

P/N Cat. No.

12-20 m 79460 5055-16510 35,000

PEEK 12-20 m 79373 7845-79373 45,000

12-20 m 79453 5055-16515 38,000

PEEK 12-20 m 79374 7845-79374 39,000

PRP-X100

P/N Cat. No. 150 x 4.1 mm I.D. 10 m 79441 5055-16400 98,000

250 x 4.1 mm I.D. 10 m 79442 5055-16405 119,000

PRP-X200

P/N Cat. No.

10 m 79456 5055-16410 35,000

PEEK 10 m 79368 7845-79368 45,000

10 m 79449 5055-16415 38,000

PEEK 10 m 79369 7845-79369 39,000

P/N Cat. No. 150 x 4.1 mm I.D. 7 m 79717 7845-79717 107,000

250 x 4.1 mm I.D. 7 m 79473 5055-16600 137,000

PRP-X400

P/N Cat. No.

PEEK 7 m 79376 7845-79376 45,000

12-20 m 79131 7845-79131 33,000

PEEK 7 m 79377 7845-79377 39,000

12-20 m 79132 7845-79132 22,000

P/N Cat. No. 150 x 4.1 mm I.D. 7 m 79855 7845-79855 107,000

250 x 4.1 mm I.D. 7 m 79828 7845-79828 130,000

PRP-X800

P/N Cat. No.

7 m 79830 7845-79830 38,000

PEEK 7 m 79831 7845-79831 52,000

7 m 79832 7845-79832 42,000

PEEK 7 m 79833 7845-79833 45,000

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P/N Cat. No. 250 x 4.1 mm I.D. 7 m 79440 5055-16700 119,000

250 x 4.6 mm I.D. PEEK 7 m 79388 7845-79388 124,000

RCX-10

P/N Cat. No.

12-20 m 79462 5055-16705 35,000

PEEK 12-20 m 79378 7845-79378 45,000

12-20 m 79463 5055-16710 37,000

PEEK 12-20 m 79379 7845-79379 39,000

P/N Cat. No. 250 x 4.1 mm I.D. 7 m 79803 7845-79803 113,000

150 x 4.6 mm I.D. PEEK 7 m 79370 7845-79370 106,000

250 x 4.6 mm I.D. PEEK 7 m 79877 7845-79877 124,000

RCX-30

P/N Cat. No. PEEK 12-20 m 79371 7845-79371 45,000 PEEK 12-20 m 79372 7845-79372 39,000

P/N Cat. No. 250 x 4.1 mm I.D. 9 m 79431 7845-79431 113,000

305 x 7.8 mm I.D. 9 m 79436 7845-79436 130,000

HC-75 (Ca2+)

P/N Cat. No. 305 x 7.8 mm I.D. 9 m 79438 7845-79438 162,000

HC-75 (Pb2+)

P/N Cat. No. 250 x 4.1 mm I.D. 9 m 79476 7845-79476 113,000

100 x 7.8 mm I.D. 9 m 79547 7845-79547 119,000

305 x 7.8 mm I.D. 9 m 79544 7845-79544 130,000

HC-75 (H+)

P/N Cat. No. 12-20 m 79133 7845-79133 33,000

12-20 m 79134 7845-79134 22,000

P/N Cat. No. 12-20 m 79866 7845-79866 36,000

12-20 m 79865 7845-79865 24,000

Anion P/N Cat. No.

PRP-X100 250 x 2.1 mm I.D. 5 m 79670 7845-79670 129,000

PRP-X100 100 x 4.6 mm I.D. 5 m 79669 7845-79669 104,000

PRP-X100 250 x 4.6 mm I.D. 7 m 79668 7845-79668 142,000

PRP-X110S 250 x 4.6 mm I.D. 7 m 79671 7845-79671 142,000

1 2.3 mm 1.0 100 mm PEEK

1 2 PEEK 1 2 1 1

5 20 x 2.3 mm I.D.PEEK 5 8 x 3 mm I.D. 2 20 x 4.6 mm I.D.

PEEK

HC-75

SILFILTER STD C18

SILFILTER STD C18 C18 ODS 2.3 mm

pH 1 ~ 7.5

Cat. No.

SILFILTER STD C18 A 2 5020-10404 35,000

SILFILTER STD C18 B 2 5020-10405 32,000

HAMILTON Polymer Column6

20 mer 21 mer

20 mer

21 mer

12 3 4 5

80 C

70 C

60 C

50 C

40 C

2550

1900

1250

600

-50

mA

U

0 2 4 6 8Time (min)

(TEAA) 20 mer 21 mer 75

APPLICATION NOTE

ConditionsColumn : PRP-1 (7 m, 250 4.1 mm I.D.)Eluent : A) 0.1 M Sodium Phosphate (pH = 11.1)

: B) CH3CN: A/B = 70/30, v/v

Flow Rate : 1.0 mL/minCol. Temp. : AmbientDetection : UV 235 nmInjection Volume : 50 LSample : 1. Hydrastine

2. Berberine

1 PS-DVB

ConditionsColumn : PRP-C18 (5 m, 100 4.6 mm I.D.)Eluent : A) 0.1 M Triethylammonium Acetate (TEAA) (pH = 7.0)

: B) TEAA/25% CH3CN: A/B = 60/40 15 min 40/60, v/v

Flow Rate : 0.75 mL/minCol. Temp. : 75 CDetection : UV 260 nmInjection Volume : 15 LSample : 1. 20 mer (5'-TCG AGC GCT AAC GTA CTT AG-3')

Oligonucleotide: 2. 21 mer (5'-TCG AGC GCT AAC GTA CTT AGA-3') Oligonucleotide

ConditionsColumn : PRP-h5 (5 m, 50 4.6 mm I.D.)Eluent : A) Deionized Water, 0.1% TFA

: B) CH3CN, 0.1% TFA: A/B = 78/22 10 min 45/55, v/v

Flow Rate : 1.0 mL/minCol. Temp. : 40, 50, 60, 70, 80 CDetection : UV 210 nmInjection Volume : 15 LSample : 1. Ribonuclease A 2. Cytochrome c

3. apo-Transferrin 4. Myoglobin 5. Carbonic Anhydrase

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Cat. No. 6001-72305 20 45

ED723 (

(

ConditionsColumn : RCX-30 (7 m, 150 4.6 mm I.D.)

Eluent : A) 60 mM Sodium Hydroxide: B) 500 mM Sodium Acetate, 60 mM Sodim Hydroxide: A/B = 100/0 4 min 100/0 - 11 min - 0/100, v/v

Flow Rate : 2.0 mL/minCol. Temp. : Ambient

Detection : Pulsed Amperometric, Dual Gold Electrode E1 = 350 mV T1 = 166 msec, E2 = 900 mV T2 = 166 msec, E3 = -850 mV T3 = 500 msec

Injection Volume : 50 LSample : 1. Glucose 2. Fructose 3. Maltose

4. Maltotriose 5. Maltotetraose 6. Maltopentaose 7. Maltohexaose 8. Maltoheptaose 9. Maltooctaose 10. Maltononaose 11. Maltodecaose

ConditionsColumn : HC-75 (Ca2+) (9 m, 305 x 7.8 mm I.D.)

Eluent : Deionized Water

Flow Rate : 1.0 mL/minCol. Temp. : 90 CDetection : RI

Injection Volume : 10 LSample : 1. Sucrose

2. Glucose 3. Fructose 4. Mannitol 5. Sorbitol

pH 7 CO2

Cat. No.

L 6010-92050 47,000

L 6010-92060 45,000

TEL. 03 (5323) 6611TEL. 06 (6357) 5060TEL. 045 (985) 7900TEL. 024 (534) 2191TEL. 029 (858) 3700TEL. 048 (667) 1611TEL. 043 (248) 2441TEL. 052 (931) 1761TEL. 082 (233) 1101TEL. 092 (738) 6633

TEL. 04 (2934) 2121TEL. 04 (2934) 1100TEL. 024 (533) 2244

FAX. 03 (5323) 6622FAX. 06 (6357) 4580FAX. 045 (985) 7901FAX. 024 (536) 1518FAX. 029 (858) 3780FAX. 048 (667) 1656FAX. 043 (248) 2485FAX. 052 (931) 1814FAX. 082 (233) 1110FAX. 092 (738) 6636

FAX. 04 (2934) 2128FAX. 04 (2934) 3361FAX. 024 (534) 2139

20130326YD5T

12

3

4

5

6 7

0 2 4 6 8 10 12Minutes

1 2

Minutes0 3 6 9 1 2

1

2 3

4 5

6

APPLICATION NOTE

PRP-X110S Anion (5 )

1 2

ConditionsColumn : PRP-X110S (7 m, 150 4.1 mm I.D.)Eluent : 1.7 mM Sodium Bicarbonate, 1.8 mM Sodium Carbonate,

0.1 mM Sodium ThiocyanateFlow Rate : 2.0 mL/minCol. Temp. : AmbientDetection : Suppressed ConductivityInjection Volume : 50 LSample : 1. Fluoride 2. Chloride

3. Nitrite 4. Bromide 5. Nitrate 6. Phosphate 7. Sulfate

ConditionsColumn : PRP-X800 (7 m, 250 4.6 mm I.D.)

Eluent : 2.0 mM Copper SulfateFlow Rate : 2.0 mL/minCol. Temp. : Ambient

Detection : UV 220 nm

Injection Volume : 50 LSample : 1. Lithium

2. Sodium 3. Ammonium 4. Potassium 5. Magnesium 6. Calcium