Скрининг mrsa, esbl. mbl (1)
DESCRIPTION
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VIM IMP MBL
- :
() 30 ;
;
16-18, 24 .
- ()
,
(0,5 )
: 30 1. 2. , 0,5
3.
4. 510 . 5. 16-18 33-35 ( ) 6. :
S. aureus ATCC 25923 mecA ( 23-29 );
S. aureus ATCC 43300 mecA- ( 21 ) , .
, - .
1. Staphylococcusspp. : ()
, ,
(mecA+) (mecA-)
S. aureus and
S. lugdunensis 30 2122
,
S. lugdunensis 30 2425
ESBL
, ESBL, . ESBL- III-IV (8-16 /), ESBL. ESBL -(- . - , ESBL III (, ), , ( /). 20-30 . , (/ /) .
E. coli ATCC 25922;
K. pneumoniae ATCC 700603 (ESBL+)
- () (90 )
,
(0,5 )
1. .
2. , 0,5 .
3. .
4. 510 1.
5. 18-20 37.
6. . MBL
-(- (-) MBL .
P. aeruginosa ATCC 27853 (MBL-)
P. aeruginosa 565 (MBL+)
2. , 15 ( ) , . 5(!) 0,5 M .
1. ESBL.
CTX , 30 , CAZ , 30 , AMC / , 20/10
Enterobacter spp., Citrobacter freundii, Serratia spp., Morganella morganii, Providencia stuartii, P.rettgeri .
2. MBL.
IPM , 10 ; MEM , 10 ; CAZ , 30
(-),
:E. coli ATCC 25922
: 10 10 .
: -.
0,9% NaCl ( )
0,5 McFarland 1. E. coli ATCC 25922 ( ) 0,5 McFarland.2. 1:10.
3. (E. coli ATCC 25922) - ( - ). ( ) 3-10 .
4. () ( ):
90 (100) 1 .
150 4 (. 1, 2).
5. (/ - , ) () . 20-25 . (. 1, 2).
6. 35 16-20 .
7. :
E. coli ATCC 25922 . (. 1, 2).
E. coli ATCC 25922 . (. 1, 2).
. , .
; >90% >90% KPC- . -- .
- .
Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Third Informational Supplement. CLSI document M100-S23. 2013. P.53-56
Rapid CARB Screen Kit (Rosco Diagnostica)( )
100 - B-PERII 1
100 .. 2
(, , .)
Rapid CARB Screen Kit:
- CARB ( + )
- CARB Negative Control1. ( ) 100 - B-PERII 4 McF;
2. 1 ;3. 30 ;4. ..: 1 , 2 ;5. 50 100 .. (2 );6. 1 CARB ( + ); 2 Negative Control. ;7. t = 35-37 30 , 1 , 2 . 2 .
1. ( )
2. 30 1 ( )
CTX FEP*
AMC
CAZ
CAZ
CTX FEP*
IPM
MEM
CAZ
E. coli ATCC 25922
E. coli ATCC 25922
E. coli ATCC 25922. K. pneumoniae ATCC BAA-1705, , , . E. coli ATCC 25922 .
1. 100 .
(1) K.pneumoniae ATCC BAA-1705,
(2) K.pneumoniae ATCC BAA-1706,
(3) ,
2. 150 .
(1) K.pneumoniae ATCC BAA-1705,
(2) K.pneumoniae ATCC BAA-1706,
(3-8) :
(3, 4, 5, 7, 8)
(6)