口 腔 病 理 科

Salivary gland-embryology唾液腺 - 胚胎學

陳玉昆副教授 : 高雄醫學大學 口腔病理科 07-3121101~2755 yukkwa@kmu.edu.tw

學 習 目 標1. Origins of salivary gland2. Stages of development 3. Differentiation of terminal tubule cells4. Cleft formation during salivary gland branching5. Factors affecting branching of salivary gland cell cords6. Classification of salivary gland cell cords7. Classification, innervation, anatomy of major salivary gland8. Classification, anatomy of minor salivary gland9. Ductal system - components, comparison, histology10. Saliva – composition, function

參考資料參考資料References

1. Avery JK. Oral development and histology. 2nd edition, Chapter 21, p. 352-812. Avery JK. Oral histology-a clinical approach. 1st edition, Chapter 15, p. 184-953. Berkovitz BK, Holland GR, Moxham BH. 2nd edition, p.215, 2204. de Almeida PDV, et al.. Saliva Composition and Functions: A Comprehensive Review. J Contemp Dent Pract 2008 3:72-805. Kaohsiung Medical University, Oral Pathology Department

Origins of salivary gland

8th prenatalweek

6th prenatalweek

Minor gland: 12 prenatal weeks

Frontal sectionthrough

developing face

Epithelial outgrowth of buccal epitheliuminvading the underlyingmesenchyme

Epithelial outgrowth of buccal epitheliuminvading the underlyingmesenchyme

Nasalseptum

TongueParotid bud

Sublingualbud

Submandularbud

7th prenatal week Ref: 1

Stages of development

Bud formation

Stage I

Ref: 1

Stages of development

Stage II

Cord growth

Ref: 1

Stages of development

Stage III

Branching of cord

Ref: 1

Stages of development

Stage IV

Lobule formation

Ref: 1

Stages of developmentStage V

Canalization of cordsto form ducts

Ref: 1

Stages of development

Lobule of gland

Connectivetissuesepta

Ref: 2

Stages of development

Stage VI

CytodifferentiationRef: 1

Differentiation of terminaltubule cells

Terminaltubule cell

Proacinar cell

Intercalatedduct cell

(Stem cell)

Acinar cells

Factors affecting branchingof salivary gland cell cords

Hyaluronidase

Collagen

Generalized cellproliferation

No branching

Basal lamina

Branching

Digest the collagen

Ref: 1

Cleft formation during salivary gland branching

A B C

M

BMEM = messenchymeBM = basement membraneE = epithelium

Type IV

Type III

Type I

Collagen Type

Ref: 1

Classification of salivary gland cell cords

Tubuloacinartype

Exocrine -duct systemfor secretion transportation

Tubular

Tubulo-acinar

Branchingduct

Main duct

Grape-likeacinar

Ref: 1

Classification of salivary gland cell cords

Method of secretoryproduction

Discharge

Membrane

Secretory granule

Golgi

Merocrinetype secretoryprocess

Ref: 1

Classification- major salivary gland

Size Location Capsule

(14-28g)

(7-8g)

(3g)

Parotid gland

Parotid gland

Subman-dibulargland

Subman-dibulargland

Sublingualgland

Sublingualgland

Ref: 1

Anatomy - major salivary gland

Stensen’s duct(7 cm long)

Warthin’s duct(5 cm long)

Bartholin’s duct(Rivinus’s ducts)

Ref: 2

Classification- major salivary glandType ofsecretion

Contribution of saliva

Striated duct

intercalated duct

Paro-tidgland

Paro-tidgland

Subman-dibulargland

Subman-dibulargland

Sublin-gualgland

Sublin-gualgland

%

%

%

Refs: 1, 4

Classification - major salivary gland

Parotid glandParotid gland

SublingualglandSublingualgland

SubmandinularglandSubmandinulargland

Sympatheticinnervation

Parasympathetic innervationPreganglionic Postganglionic

Ref: 1

Innervation of salivary gland

Parotidgland

Sublingualgland

Submandibular gland

Auriculotemporal n

Chordatympani

Oticganglion

Submandibularganglion

Superiorcervicalganglion

Superiorsalivary nucleus

Inferiorsalivary nucleus

VII

IX

Sympathetic

Ref: 1

Parasympathetic

Anatomy- major salivary gland

Facial nerve vs fetal parotid glandFacial nerve vs fetal parotid gland

Ref: 1

Classification- major salivary gland

Parotid glandParotid gland

Submandinulargland

Submandinulargland

Sublingualgland

Sublingualgland

Blood supplyArterial Venous

Ref: 1

Anatomy - minor salivary glandUncapsulated;named by location

Ref: 2

Classification- minor salivary glandName Location Type of secretion

Contribution of saliva: 5-10%

Ref: 1

Classification- minor salivary gland

Ref: 3

A: Small serous glands of von Ebner empty into the base of trench

Taste buds

Circumvallate papillae in longitudinal

AA

Taste budsTaste buds

Ductal system

Ref: 3

Acinus

Capsule

Septa

Lobe

Secretory Units

Intercalated

StriatedExcretory

Ducts

Ductal system

Ref: 3

Intra-Lobular

Striatedduct

Excretoryduct

Serous cell

Serousdemilune

Mucousacinus

Mucouscell

Intercalcatedduct

Myoepithelial cellInter-Lobular

Ductal systemOral cavity Major salivary gland duct orifice

Oral epithelium

Interlobularduct system

Intralobularduct system

Excretory duct

Mucousacinus

Mixedacinus

Serousacinus

Ref: 2

Comparison of ductal systemRelative ductal length

Parotid glandParotid gland

Submandinular glandSubmandinular gland

Sublingual glandSublingual gland

Key

Excretory

Striated

Intercalated

Intercalated duct:P > M > L

Striated duct:M > P > L

Ref: 1

Histology of ductal system

Intercalated duct

Excretory duct

Myoepithelial cells

Mucous acini

Serous demilune

Mixed acini

Striated duct

12

Ref: 1

Histology of ductal systemMixed alveolus

Mucous alveolus

Serous alveolus

Serouisdemilune

Ref: 2

Histology of ductal system

Ref: 2

Histology of ductal system

Ref: 2

Histology of ductal system

Myoepithelialcell

Myoepithelialcell

Mucouscell

Ref: 2

Histology of ductal system

Ref: 5

Intercalated duct

Myoepithelialcell

Serousacini

Excretory duct

Adipose tissue

Histology of ductal system

Ref: 5

Myoepithelialcell

Serousacini

Striated duct

Mixedacini

Mucousacini

Histology of ductal system

Intralobularduct

Bloodvessel

Ref: 2

Histology of ductal system

Scanningelectronmicroscope

Myoepithelialcell processes

Ref: 2

Histology of ductal system

Serous cell Mucous cell

Ref: 2

Composition of saliva

Ref: 1

ml

Composition of saliva

Ref: 2

Composition of saliva

Ref: 2

Function of saliva

1

2

3

4 5

6

Ref: 2

3 pints = ? liter

Function of saliva

Ref: 2

Function of saliva

Refs: 2, 4

Function of saliva

Ref: 2

Summaries

Knowing: 1. Origins of salivary glands2. Stages of development 3. Differentiation of terminal tubule cells4. Cleft formation during salivary gland branching5. Factors affecting branching of salivary gland cell cords6. Classification of salivary gland cell cords7. Classification, innervation, anatomy of major salivary gland8. Classification, anatomy of minor salivary gland9. Ductal system - components, comparison, histology

10. Saliva – composition, function