動物模式核心實驗室

•Tumorgenesis nude mice model

• Disease animal model

•Gene therapy model

•Drug delivery animal model

研究技術 /方法

蛋白質體核心實驗室

• 2D gel electrophoresis

• Protein identification:Tandem mass spectrometry [ESI/Q-TOF, MALDI-Q-TOF]: protein identification

• Structural proteomics:fluorescence spectrophotometry, gel filtration, site-directed mutagenesis, circular dichrorism

• Recombinant protein production

• Protein purification:FPLC ,MDLC

• Protein-protein interaction: real-Time biomolecule interaction analysis

• Protein-protein interaction:yeast two-hybrid system

研究技術 /方法

細胞分子生物核心實驗室

•Laser capture microdissection •Immunohistochemistry•Electron microscope•Cell culture•Western blot•Northern blot•Immunoprecipitation•Transfection: transient, stable•Electrophoretic mobility shift assay (EMSA)•ChIP Chromatin immunoprecipitation (CHIP)•Promoter activity assay•RNAi construct•Confocal microscope•Flow cytometry

研究技術 /方法

基因體核心實驗室

•Microarray

•Real-time PCR

•DNA and RNA extraction

•Gene subtraction

•Serial analysis of gene expression (SAGE)

•Genotyping (DNA polymorphism)

研究技術 /方法

生物資訊暨結構生物學核心實驗室

•Microarray data analysis

•SNP data analysis

•Genome bioinformatics

•sequence analysis

•Design of primers

•Drug design

•Theoretical calculation of small molecules and macromolecules

•Structural identification of small molecules and macromolecules

研究技術 /方法