06 aranzana
TRANSCRIPT
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Traits
Sub-acid taste(D)
Yellow/White flesh (Y)
Peach/nectarine skin (G)
Flat/round Shape (S)
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Traits
Sub-acid taste(D)
Yellow/White flesh (Y)
Peach/nectarine skin (G)
Flat/round Shape (S)
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Traits
Sub-acid taste(D)
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Traits
Sub-acid taste(D)
In general, the consumer acceptance is greater for sub-acid than for acid cultivars
Sub-acid trait is especially important in early varieties: have little time on the tree in between bloom and harvest time, that they don’t have the ability to build up sugars. Sub-acid compensate this lack of sugars
Sub-acid cultivars do not cover all the production season
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Sub-acid taste(D)
One gene responsible for the sub-acid trait
The sub-acid allele is dominant
Parents sub-acid 75% -100%
One parent sub-acid 50% -100%
X
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Sub-acid taste(D)
1580 peach varieties, from different origins genotyped with 9,000 SNP
SNP_IGA_546094
Haplotypes (combination of SNPs) in D locus
Which are associated with the trait?
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Sub-acid taste(D)
* Possibility of some phenotypic errors
TraitIRTA1IRTA
2IRTA
4IRTA
5IRTA
6IRTA
7IRTA
8IRTA
9IRTA10
IRTA11
IRTA12
IRTA13
IRTA14
Average of
success
Notes
D 100% 80% 97% 98% 92% 97% 89% 85% 97% 95% 86% 95% 98% 93%G allele
associated with the SAc
Large proportion of samples out or type
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Trait
RDG_pop1
RDG_pop2
RDG_pop3
RDG_pop4
RDG_pop5
RDG_pop6
RDG_pop7
RDG_pop8
RDG_pop9
RDG_pop10
RDG_pop11
RDG_pop12
Average
D 90 87 93 93 95 - - - 98 83 93 92 92 %
Sub-acid taste(D)
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Trait ASF_pop1
ASF_pop2
ASF_pop3
ASF_pop4
ASF_pop5
ASF_pop6
ASF_pop7
Average
D 89 88 81 87 95 … … 88 %
Sub-acid taste(D)
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Sub-acid taste(D)
• Information on the SNP in international databases
• Facilitated to FB MAB services
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Flat/round Shape (S)
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High consumer demand
Breeding efforts required to fulfill quality requirements
Flat/round Shape (S)
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• Flat allele is dominant• Flat need to be in heterozygosis (fruits in
homozygous genotypes abort)
GeneticsSNPs in WP4Pilot studiesUse of SNPs
Flat/round Shape (S)
Both parents flat
One parent flat
X
50% Flat 25% Round25% No fruit50% Flat 50% Round
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1580 peach varieties, from different origins genotyped with 9,000 SNP
Haplotypes (combination of SNPs) in D locus
Which are associated with the trait?
Flat/round Shape (S)
A A G
A A A
G G G
G A G
A G A
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Flat/round Shape (S)
Trait
IRTA1
IRTA2
IRTA4
IRTA5
IRTA6
IRTA7
IRTA8
IRTA9
IRTA10
IRTA11
IRTA12
IRTA13
IRTA14
Average of
successNotes
S ns 88% ns 99% ns ns ns ns ns 99% ns ns 99% 96% Flat allele: AAG
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Flat/round Shape (S)
Trait ASF_pop1
ASF_pop2
ASF_pop3
ASF_pop4
ASF_pop5
ASF_pop6
ASF_pop7
Average
s 100 - - 96 99 … … 98 %
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Summary
Sub-acid taste(D): 1 SNP
Yellow/White flesh (Y): 2 SNPs + 1SNP or INDEL
Peach/nectarine skin (G): 4 SNPs not completely linked or INDEL
Flat/round Shape (S): 3 SNPs highly linked