1 cs-rfa-evbsd...cs-rfa-evbsd 11998 bp mlui 228 noti 1521 noti 2698 ecori 3019 mlui 3466 psti 4141...
TRANSCRIPT
CS-RfA-EVBsd11998 bp
MluI 228
NotI 1521
NotI 2698
EcoRI 3019
MluI 3466
PstI 4141AgeI 4375
ApaI 4894
AgeI 5468
PstI 5687ApaI 6257XhoI 6669
ApaI 7980
CMV RU55' SD
RRE
3' SAcPPT+CTS
RfA
attR1
Cm
ccdBattR2
EF-1Venus
T2ABsdPRE
del U3R
U5BGH pA
f1 ori
SV40 pro/ori
Zeo
SV40 pA
ColE1 ori
Amp 1
Miyoshi
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Plasmid name: CS-RfA-EVBsd Plasmid size: 11998 bp Note CMV: Human cytomegalovirus immediate early promoter y: Packaging signal 5’SD: 5’ splicing donor site 3’SA: 3’ splicing acceptor site RRE: Rev responsive element cPPT: Central polypurine tract CTS: Central termination sequence RfA: Gateway Reading Frame Cassette A containing Cm, ccdB, attR1, and attR2 Cm: Chloramphenicol resistance gene ccdB: The ccdB gene encodes a protein that interferes with E. coli DNA gyrase attR1, 2: Sites for site-specific recombination with a Gateway vector (Invitrogen). attR sites always recombine with attL sites in a reaction mediated by the LR Clonase (Invitrogen). EF-1: Human elongation factor 1 α subunit promoter Venus: A variant of yellow fluorescent protein (YFP) gene T2A: Thosea asigna virus 'self-cleaving' 2A peptide sequence Bsd: Blasticidin resistance gene PRE: Woodchuck hepatitis virus posttranscriptional regulatory element del U3: Deletion of enhancer and promoter sequences in the U3 region BGH pA: Bovine growth hormone polyadenylation signal SV40 pro & ori: SV40 early promoter and origin Zeo: Zeocin resistance gene SV40 pA: SV40 polyadenylation signal Amp: Ampicillin resistance gene *Use the DB3.1 or ccdB Survival 2 T1R E. coli strain for propagation of plasmids containing the ccdB gene.
Miyoshi
テキストボックス
y