1st israeli isac - weizmann institute of science · 2019. 12. 12. · zosia maciorowski, isac live...

1st Israeli ISAC Flow Cytometry workshop 1

מדע לרווחת האנושות

Science for the benefit of humanity

December 15-17, 2019Weizmann Institute of Science

Rehovot, Israel

1st Israeli ISAC Flow Cytometry

workshop

• Customized solutions for deep high-parameter cell analysis

• High-end low noise VPX electronics with advanced signal processing features

• Familiar BD FACSDiva™ software on Windows 10 / 64 bit

• Up to 5 lasers with spatially separated laser intercepts

• Experiment specific adjustable laser power

• Compact Polygon/Decagon arrays with upgraded PMT detectors

• Specialized high-parameter application team to support youwith panel design and to answer your cell analysis questions

• Optional automated sample processing in high-throughput mode

• Profit from FlowJo™ high-dimensional software analysis tools

Push your Research to the Limits BD FACSymphonyTM A3 AnalyzerBring state-of-the-art technology into your scienceHigh-parameter flow cytometry is a powerful analytical tool that enables scientists to identify and analyze distinctive phenotypes in heterogeneous populations. BD Biosciences actively supports groundbreaking immunological research with flow cytometry for over 40 years. The BD FACSymphony A3 flow cytometer is a high-parameter system (HPS) that leverages the inherent benefits of flow cytometry for deep exploration science. The BD FACSymphony A3 analyzer allows the simultaneous measurement of up to 30 . BD exclusively delivers the experience in high-parameter panel design support into your lab.

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Photo: ©Sergey Nivens - stock.adobe.com 197305360© 2019 BD. BD, the BD Logo and all other trademarks are property of Becton, Dickinson and Company.


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For more information and accessibility issues please contact: [email protected]

The workshop is supported by the International Society for Advancement of Cytometry (ISAC) Education Task force

Registration

John Nolan, Scintillon Institute, USAJoe Trotter, senior scientist, BD Geoff Osborne, SORP program, BDJustine Sinnaeve, Vanderbilt University, USASierra Barone, Vanderbilt University, USADavid Hedley, University of Toronto, CanadaSue Chow, University of Toronto, CanadaZosia Maciorowski, ISAC live education task forceZiv Porat, Weizmann Institute of ScienceHadas Keren-Shaul, Weizmann Institute of scienceJohn Wotherspoon, senior scientist, BD

Organizing Committee

Ziv Porat, Weizmann Institute of ScienceAmir Grau, Technion – Israel Institute of Technology Amit Tzur, Bar Ilan UniversityUzi Hadad, Ben-Gurion University of the NegevZosia maciorowski, ISAC Live Education Task ForceTomas Kalina, ISAC Live Education Task Force

Invited Speakers

Topics

Department of Immunology

Sponsors

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On behalf of the Organizing Committee, we are pleased to welcome you to the 1st Israeli Flow Cytometry Workshop. This workshop is a joint effort with the International Society for the Advancement of Cytometry (ISAC) Educational Task Force, and flow cytometry core facilities of the Weizmann Institute, the Technion, and Ben-Gurion University. We have done our best to put together a program to suit your different levels and varied interests, with a good grounding in the basics up to the latest techniques in flow cytometry. We have highly knowledgeable and enthusiastic faculty, some of the best in their field, who are very approachable. Do not hesitate to ask questions and engage them in conversation inside or outside of the sessions! This workshop, and all of our ISAC Cytometry workshops worldwide, could not take place without the generous support and involvement of our sponsors, from the Weizmann Institute, Ben-Gurion University and commercial companies. Their funding allows us to bring you outstanding faculty from around the world, and to give you access to the latest instrumentation. Their application specialists are an important fund of knowledge we all should tap into. Please use this opportunity to consult with them and attend their talks. We also take this opportunity to thank those here at the Weizmann Institute, in particular the Flow Cytometry Unit staff, who made the organization much less painful with their generous help and advice. And of course, deepest thanks to the faculty who have come from afar to donate their time and expertise to teach us. Learn, enjoy the workshop and « Go with the Flow » !

Ziv Porat, The Weizmann InstituteAmir Grau, The Technion - Israeli Institute for TechnologyUzi Hadad, Ben-Gurion University of the Negev Zosia Maciorowski, Institut Curie

Welcome

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Our Flow Cytometry Support Team would gladly help you with your research challenges. For more information please contact Orit: 08-9558848

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15 DECEMBER 2019Candiotty lecture hall

08:30 Registration

09:00 Introduction and opening remarks

09:15 Multicolor Panel Design-Zosia Maciorowski

10:00 Sample Preparation - John Wotherspoon

10:45 Coffee Break

11:00 Advanced methods - Cell signaling - David Hedley

11:45 Advanced Cell Sorting - Geoff Osborne

12:30 Lunch

13:30 Single Cell Sorting and RNA seq - Hadas Keren-Shaul

14:15 Computational Cell Sorting - Joe Trotter

15:00 Coffee Break

15:15 Extracellular Vesicles - John Nolan

16:00 Imaging Flow Cytometry - Ziv Porat

Program

1st Israeli ISAC Flow Cytometry workshop8

16 DECEMBER 2019Practical Modules

Multicolor Track (3 modules) ZE5 - FACS unit

Cell Sorting Track (3 modules) SORP ARIAs - FACS unit

High Dimensional Data Analysis Track (2 modules) Levin 101 / Benoziyo 290

09:00 Multicolor Track session 1 Zosia

Cell Sorting Track session 1 Geoff

HDDA Track session 1 Justine/Sierra

10:30 Coffee10:45 Multicolor Track

session 1 John/ZosiaCell Sorting Track session 1 Hadas



12:15 Lunch - Candiotty13:00 BD lecture - Present and future of high parameter cytometry for

immunophenotyping - Candiotty hall13:30 Biological Industries lecture - Inherited and transient phenotypic variability -

Candiotty Hall13:45 Multicolor Track

session 1 John/ZosiaCell Sorting Track session 1 Joe

Cell Sorting Track session 2 Hadas



session 2 ZosiaCell Sorting Track session 2 Joe



17 DECEMBER 2019Practical Modules09:00 Multicolor Track




session 2 John/Zosia

Multicolor Track session 3 Zosia

Cell Sorting Track session 3 Joe



12:15 Lunch - Wolfson13:00 Rhenium lecture - From Sample to Result - A Full Workflow for Flow

Cytometry - Wolfson Hall13:30 Lumitron - Advanteon Flow Cytometer -

Advancing the Boundaries of Flow Cytometry - Wolfson hall13:45 Multicolor Track

session3 John/ZosiaCell Sorting Track session 4 Hadas

HDDA Track troubleshooting




16 DECEMBER 2019Practical Modules

Multicolor Track (3 modules) ZE5 - FACS unit

Cell Sorting Track (3 modules) SORP ARIAs - FACS unit

High Dimensional Data Analysis Track (2 modules) Levin 101 / Benoziyo 290

09:00 Multicolor Track session 1 Zosia







12:15 Lunch - Candiotty13:00 BD lecture - Present and future of high parameter cytometry for

immunophenotyping - Candiotty hall13:30 Biological Industries lecture - Inherited and transient phenotypic variability -

Candiotty Hall13:45 Multicolor Track


Cell Sorting Track session 2 Hadas



session 2 ZosiaCell Sorting Track session 2 Joe



17 DECEMBER 2019Practical Modules09:00 Multicolor Track




session 2 John/Zosia

Multicolor Track session 3 Zosia

Cell Sorting Track session 3 Joe



12:15 Lunch - Wolfson13:00 Rhenium lecture - From Sample to Result - A Full Workflow for Flow

Cytometry - Wolfson Hall13:30 Lumitron - Advanteon Flow Cytometer -

Advancing the Boundaries of Flow Cytometry - Wolfson hall13:45 Multicolor Track

session3 John/ZosiaCell Sorting Track session 4 Hadas

HDDA Track troubleshooting



Cell Signaling (1 module) Benoziyou basement / room 56

Evs (1 module)Benoziyou basement / room 56

Imaging Flow Cytometry (1 module) Wolfson floor -2

Cell Signaling session 1 Hedley/Chow

Imaging Flow session 1 Ziv

Evs session 1 John Nolan











SPEAKERS

Geoffrey Osborne

Director of Special Order Research Products for BD Biosciences, based San Jose, CA, USA. He leads a team focused on meeting customer needs by engineering technology advances to modify flow cytometers to fit unique

roles. Prior to taking his current role He was Director of Flow Cytometry for both Queensland Brain Institute and the Australian Institute for Bioengineering and Nanotechnology, at the University of Queensland, Brisbane, Australia for 13 years. In this position Mr Osborne lead a team that provided crucial cell sorting and analysis services to researchers both within QBI and across the broader university. The laboratory specialised in the analysis and separation of cells derived from a variety of sources such as solid tissue, blood and cultured cell lines. In addition he held a Faculty research position where his interests were in the application of flow cytometry in stem cell research, brain tumours, novel assay development encompassing not only biological assays but also hardware / software development projects.

Hadas Keren-Shaul

Hadas Keren-Shaul earned her BSc in biotechnology and Food engineering from the Technion (2004) and her PhD in human molecular genetics and biochemistry from Tel Aviv University (2011). After conducting postdoctoral

research at the Weizmann Institute between 2012 and 2016, she joined the Institute as a Staff Scientists. Since 2017, she has served as the Head of the Genomics, “Sandbox” unit at the Weizmann Life Science Core Facility that offers Institute researchers state-of-the-art genomic tools for biological projects. Since 2019, she is also heading the Crown Institute for Genomics at the Nancy and Stefan Grand Israel National Center for personalized medicine. Throughout her academic career, Dr. Keren-Shaul has been fascinated by the development of new technological tools for the advancement of science. During her postdoc, in which she focused on the immune system’s role in Alzheimer’s disease, she developed MARS-seq, a protocol that supports the preparation of libraries for RNA sequencing at high efficiency, high throughput and low cost, and can be used with very small amount of genetic material. Making it possible to sample and label mRNA molecules form a thousand individual cells in a single experiment, this technique facilitates the functional sorting of different cell populations within a variety of biological samples. Such “massively parallel” experimental protocols have contributed significantly to biomedical discovery. To give just one example, Dr. Keren-Shaul have utilized the MARS-seq platform, with Weizmann prof. Ido Amit and Michal Schwartz, for the discovery of a subpopulations of immune cells in the brain that could potentially serve as targets for future Alzheimer’s disease therapies.


John Nolan

John Nolan received BS degrees (Biology and Chemistry) from the University of Illinois and a PhD (Biochemistry) from Penn State University. He did post-doctoral research Los Alamos National Lab, where he later served as Technical

Staff Member and Director of the National Flow Cytometry Resource. Since 2014, he has been a Professor at the Scintillon Institute in San Diego, where his group develops new instrumentation, reagents, and assays for cytometry and single cell analysis. Recently, his group has been developing quantitative and reproducible single vesicle analysis tools for the study of extracellular vesicles (EVs). He is on the editorial boards of Cytometry and Current Protocols in Cytometry, past-President of the International Society for Advancement of Cytometry, and Fellow of the American Institute of Medical and Biological Engineering.

David Hedley

David Hedley completed his higher specialist training in medical oncology, combined with a graduate program in tumour immunology, at the Royal Marsden Hospital, University of London. He was junior faculty at the University

of Sydney, Australia, 1981-89 where he was responsible for the development of flow cytometry applications to cancer biology, including the technique for DNA content analysis using paraffin-embedded tissue that played a major role in the early development of clinical flow cytometry. Since 1990 he has been Senior Scientist/Senior Staff Physician at the Princess Margaret Hospital, and Professor of Medicine at the University of Toronto, Canada, with a major focus on pancreatic cancer. His laboratory makes extensive use of patient-derived xenografts that recapitulate the clinical spectrum of the disease. It develops flow cytometry techniques to study complex biological processes linked to experimental treatment development, and for patient monitoring during early phase clinical trials. In 2015 Dr Hedley received the International Society for the Advancement of Cytometry’s Fulwyler Award for innovative excellence in flow cytometry.

Sue Chow

Sue Chow graduated in molecular biology from the University of Toronto, and developed a specialist interest in flow cytometry, joining David Hedley’s lab as senior technician in 1990. David and Sue have published extensively in the area

of flow cytometry applications, including the measurement of lipid peroxidation, antioxidant regulation, signal transduction analysis, and epigenetic targeting. For the past 15 years they have been teaching the annual flow cytometry research methods course that alternates between the University of New Mexico and Bowdoin College ME.


Joe Trotter

Joe Trotter began his career in Flow Cytometry while in the Cell Biology Laboratories of the late Robert Holly and Renato Dulbecco at the Salk Institute in La Jolla, California. In 1971 Robert Holly wanted to study cell cycle using a

new instrument, the Flow Micro Fluorimeter (FMF) designed and built at Los Alamos National Laboratory, whereby DNA content could be measured in flow by staining fixed cells with mithramycin. There were no commercial cytometers at the time, so Joe and a small team began assembling their own FMF analyzer and Cell Sorter in the early 1970’s at Salk modeled after the Los Alamos cytometer designs of Mack Fulwyler, and others. Joe hasn’t stopped doing flow cytometry since. In the mid 1990’s he moved to The Scripps Research Institute and took on the role of flow core facility Director focusing on immunology applications until joining BD Biosciences in 2000. At BD he has been heavily involved in the development of software and multiple cytometer platforms such as the LSR II / Fortessa family, BD Influx, and the FACSMelody™ cell sorter. Currently, his role is Principal Scientist / BD Fellow within the BD R&D Advanced Technology Group focusing on new technologies and computational flow cytometry.

Dr. John S Wotherspoon

Dr. John S Wotherspoon pursued his interest in immunology as a research student at Sydney University where he completed both MSc and PhD studies in transplant immunology and graft rejection (1982-88). This coincided with

the introduction of flow cytometry platforms and monoclonal antibody technologies. During this time, he established the first cell sorter platform at the Centre for Immunology. Practical experience in immunochemistry, fluorochrome conjugation, and antibody titration provided the foundation experience to support the development of staining and application protocols needed by research collaborators. In 1988, he took an opportunity to join the local team of BD Immunocytometry Systems, Australia, as Applications Specialist. Across the subsequent 28 years, mostly in Asia, he had roles of increasing responsibility to develop the flow cytometry business. Throughout this time, his focus was capability training and education in flow technologies to improve clinical and academic research studies. Key programmes supported were the ‘Good Laboratory Practice Resource’ to improve accurate and reliable CD4 testing in local laboratories supporting HIV patient care; and the BD Horizon Multicolour education workshops.

Justine Sinnaeve

Justine Sinnaeve is a 6th year PhD student in the laboratory of Dr. Rebecca Ihrie at Vanderbilt University in Nashville, Tennessee, in the United States. She has five years experience with both fluorescence and mass flow cytometry,

working at the cutting edge of solid tumor dissociation, mass cytometry on solid tissues, and advanced data analysis techniques. Her most recent work is available on bioRxiv and details the use of a brain tumor-specific mass cytometry panel and a novel, automatic algorithm to reveal prognostic cell subsets in glioblastoma patient tumors.


Sierra Barone

Sierra Barone is the data science research assistant in the laboratory of Dr. Jonathan Irish at Vanderbilt University in Nashville, Tennessee, in the United States. She has a background in physics and experience programming in

MATLAB, Python, and R. Her primary work in the lab centers around writing streamlined, automated scripts in R for a variety of projects including 48-plex fluorescence barcoding, spectral flow cytometry, and 34+ dimensional mass cytometry analysis to reveal novel biology in human disease.

Dr. Ziv Porat

Dr. Ziv Porat is an associate staff scientist and head of the Weizmann Institute Flow Cytometry unit (the largest in Israel). His field of expertise is flow and Imaging flow cytometry, which combines the advantages of flow cytometry

and microscopy. During the last 8 years, he worked closely with more than 300 researchers, resulting in more than 70 publications, including as being the corresponding author. His record includes lecturing at Weizmann as well as all other universities in Israel, research institutes, hospitals, and biotech companies. He initiated, organized and lectured in the annual Israeli Imaging Flow Cytometry user meetings. In addition, he gained international recognition and lectured in many international conferences, and was recently selected for the prestigious “SRL emerging leaders” program of the International Society for the Advancement of Cytometry (ISAC). He is the co-founder of the Flow Cytometry Society of Israel and active in promoting education, organizing workshops and conferences.

Zosia Maciorowski

Zosia Maciorowski received her B.Sc. in Microbiology from McGill University in Montreal and M.A. in Biology from Wayne State University in Detroit. She has worked in many labs and countries over the years on a variety of different

subjects, from the early days of tissue culture to small animal surgery, monoclonal antibody production and early immunological and molecular biology techniques. In the 80’s she specialized in solid tumor preparation for multicolor and cell cycle analysis. For the last 20 years she has been responsible for the Flow Cytometry Core Facility at the Curie Institute in Paris, France. Zosia is Chair of the Education Committee of International Society for Advancement of Cytometry (ISAC) in which capacity she has led the education efforts in cytometry. She has participated in international flow cytometry workshops organized by the Live Education Task Force of ISAC in ASEAN Countries, China, India and the US.


ZE5 - unitSORP ARIAs - unit Candiotty lecture hall

Levin room 101

Benoziyo – room 56 (Basement)

Benoziyo – seminar room 290c

De Picotto room 25 Wolfson lecture hall

FACS unit - Wolfson room 21

1st israeli isac - weizmann institute of science · 2019. 12. 12. · zosia maciorowski, isac live...

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