©2000 timothy g. standish electrophoresis timothy g. standish, ph. d

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©2000 Timothy G. Standish Electrophores Electrophores is is Timothy G. Standish, Ph. D.

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Page 1: ©2000 Timothy G. Standish Electrophoresis Timothy G. Standish, Ph. D

©2000 Timothy G. Standish

ElectrophoresisElectrophoresis

Timothy G. Standish, Ph. D.

Page 2: ©2000 Timothy G. Standish Electrophoresis Timothy G. Standish, Ph. D

SU

GA

R-P

HO

SP

HA

TE

BA

CK

BO

NE

H

P

O

HO

O

O

CH2

HOH

P

O

O

HO

O

O

CH2

H

P

O

OH

HO

O

O

CH2

NH2

N

N

N

N

O

O

NH2N

NH

N

N

N O

NH2

N

B A

S E

S

DDNNAA

OH

P

O

HO

O

O

CH2

HO

O

H 2N

NHN N

N H

H

P OH

O

O

CH2

OO

N

O

H 2N

NH

H2O

H OH

P

O

OH

O

O

CH2

CH 3

O

O

HNN

H2O

Page 3: ©2000 Timothy G. Standish Electrophoresis Timothy G. Standish, Ph. D

©2000 Timothy G. Standish

The Watson - Crick The Watson - Crick Model Of DNAModel Of DNA

Majorgroove

Minorgroove

A T

T AG C

C G

C GG C

T A

A T

G CT A

A TC G

--

-

-

---

--

--

--

-

--

--

-

---

--

--

--

-

-

Page 4: ©2000 Timothy G. Standish Electrophoresis Timothy G. Standish, Ph. D

©2000 Timothy G. Standish

Submarine Gel ElectrophoresisSubmarine Gel Electrophoresis

Gel BoxNegativeelectrode

Positiveelectrode

Sample to be loaded

Well Gel

Page 5: ©2000 Timothy G. Standish Electrophoresis Timothy G. Standish, Ph. D

©2000 Timothy G. Standish

Submarine Gel ElectrophoresisSubmarine Gel Electrophoresis

Page 6: ©2000 Timothy G. Standish Electrophoresis Timothy G. Standish, Ph. D

©2000 Timothy G. Standish

100 V

Submarine Gel ElectrophoresisSubmarine Gel Electrophoresis

+-DNA Migration

Page 7: ©2000 Timothy G. Standish Electrophoresis Timothy G. Standish, Ph. D

©2000 Timothy G. Standish

Gel ElectrophoresisGel Electrophoresis

Wells

Page 8: ©2000 Timothy G. Standish Electrophoresis Timothy G. Standish, Ph. D

©2000 Timothy G. Standish

Gel ElectrophoresisGel Electrophoresis

Wells

Page 9: ©2000 Timothy G. Standish Electrophoresis Timothy G. Standish, Ph. D

©2000 Timothy G. Standish

Gel ElectrophoresisGel Electrophoresis

+

-

Direction of

DNATravel

Wells

Small

Large

Page 10: ©2000 Timothy G. Standish Electrophoresis Timothy G. Standish, Ph. D

©2000 Timothy G. Standish

Electrophoresis of DyesElectrophoresis of Dyes

1 2 3 4 5

Page 11: ©2000 Timothy G. Standish Electrophoresis Timothy G. Standish, Ph. D

©2000 Timothy G. Standish