3 ymc application data - mz-at.de · 5 index 医薬品、生体関連物質 drugs, metabolites...

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目　次 Contents

分野別インデックス List of Applications 2

APPLICATION DATA

1 .ビタミン Vitamins 8

2 .糖類 Carbohydrates 20

3 .核酸 Nucleic acids 34

4 .アミノ酸、ペプチド、タンパク質 Amino acids, Peptides, Proteins 39

5 .有機酸、脂肪酸誘導体 Organic acids, Fatty acid derivatives 52

6 .食品添加物 Food additives 54

7 .天然物 Natural products 60

8 .ステロイド Steroids 71

9 .医薬品、生体関連物質 Drugs, Metabolites 81

10.抗菌剤、抗生物質 Antibiotics 100

11.農薬、環境関連物質 Pesticides, Environmental 111

12.その他 The others 118

13.光学異性体 Optical isomers 126

化合物名インデックス Index of Analytes 131

YMC APPLICATION DATAYMC APPLICATION DATA

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ビタミンVitamins

糖類Carbohydrates

核酸Nucleic acids

アミノ酸、ペプチド、タンパク質

Amino acids, Peptides, Proteins

有機酸、脂肪酸誘導体Organic acids,

Fatty acid derivatives

食品添加物Food additives

天然物Natural products

ステロイドSteroids

医薬品、生体関連物質Drugs,Metabolites

抗菌剤、抗生物質Antibiotics

農薬、環境関連物質Pesticides,

Environmental

その他The others

光学異性体Optical isomers

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分野別インデックス List of Applications

ビタミン Vitamins

脂溶性ビタミン Oil-soluble vitamins 8

ビタミンＡ脂肪酸エステル Vitamin A fatty acid ester 8

レチノール Retinol 8

カロテン Carotene 8

β-カロテン β-Carotene 9

ビタミン D Vitamin D 9

ビタミン D2とビタミンD3 Vitamin D2 and Vitamin D3 10

トコフェロール Tocopherols 10,11,12

トコフェロールおよび酢酸トコフェロール Tocopherol and tocopherol acetate 12

ビタミン K Vitamin K 12

水溶性ビタミン Water-soluble vitamins 13,14,15,16,17

市販ドリンク剤中の水溶性ビタミン A drink including vitamins 13,14

アラントインおよび水溶性ビタミン Allantoin and water-soluble vitamins 17

尿素、アラントインおよび水溶性ビタミン Urea, allantoin and water-soluble vitamins 17

ビタミンB1誘導体 Thiamine and its modifide compounds 17

リボフラビンおよび関連化合物 Riboflavin and related compounds 18

ニコチン酸誘導体 Nicotinic acid and derivatives 18

パントテニルアルコール Pantothenyl alcohol 18

補酵素A誘導体 Coenzyme A and derivatives 18

L-アスコルビン酸およびエリソルビン酸 L-Ascorbic acid and erythorbic acid 19

L-アスコルビン酸の2,4-DNPH誘導体 2,4-Dinitrophenylhydrazone of L-ascorbic acid 19

糖類 Carbohydrates

単糖及び二糖類 Monosaccharides and disaccharides 20

単糖類 Monosaccharides 20

不飽和コンドロニ糖 Unsaturated chondro-disaccharides 21

糖アルコール Sugar alcohols 21

パラチノースとパラチニット Palatinose and palatinit 21

糖類および甘味料の一斉分析 Sugars and sweeteners 22

市販キャンディー中の糖類 Sugars in hard candy 22

オリゴ糖 Oligosaccharides 22,23,24

フルクトオリゴ糖 Fructooligosaccharides 23

市販清涼飲料水のオリゴ糖 Oligosaccharides of soft drink 23

マルトオリゴ糖(G1～G7) Maltooligosaccharides(G1～G7) 24

マルトオリゴ糖(G11～G15) Maltooligosaccharides(G11～G15) 25,26

マルトオリゴ糖(G1～G15) Maltooligosaccharides(G1～G15) 26

サイクロデキストリン Cyclodextrins 26,27,28

キチンオリゴ糖 N-Acetyl-chitooligosaccharides 28

ピリジルアミノ化(PA)糖 Pyridylamino(PA)-Sugar chains 28,29

ポリデキストロース Polydextrose 29

プルラン Pullulans 30

コンドロイチン Chondroitin 31

コンドロイチン硫酸Ａ Chondroitin sulfate A 31

コンドロイチン硫酸Ｂ Chondroitin sulfate B 31

コンドロイチン硫酸Ｃ Chondroitin sulfate C 31

ヒアルロン酸 Hyaluronic acid 32

ヘパリン Heparin 32

ヘパラン硫酸 Heparan sulfate 32

ケラタン硫酸 Keratan sulfate 32

ケラタンポリ硫酸 Keratan polysulfate 33

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INDEX核酸 Nucleic acids

核酸塩基・ヌクレオシド Nucleosides and bases 34

核酸塩基 Nucleic acid bases 34

オロチン酸 Orotic acid 35

ヌクレオシド Nucleosides 35

ヌクレオチド Nucleotides 35,36

オリゴヌクレオチド Oligonucleotides 36

オリゴヌクレオチド，poly(A) Poly(A) 37

糖ヌクレオチド Sugar nucleotides 37

プラスミドpBR322の制限酵素Msp I分解物 Plasmid pBR322 restriction fragments 38

アミノ酸、ペプチド、タンパク質 Amino acids, Peptides, Proteins

アミノ酸 Amino acids 39

市販ドリンク剤中のタウリン Taurine in nutritious drink 39

PTC-アミノ酸 PTC-amino acids 39

PTH-アミノ酸 PTH-amino acids 39

NBD-アミノ酸 NBD-amino acids 40

酸化型および還元型グルタチオン Glutathione, oxidized and reduced form 40

ペプチドマップ Peptide mapping 40

アンジオテンシン Angiotensins 41

インスリン Insulins 41,42

ペプチド Peptides 42,43,44,45,49

ペプチド -LC/MS Peptides -LC/MS 43

ペプチドおよびタンパク質 Peptides and proteins 45

タンパク質 Proteins 45,46

ヒト免疫グロブリン Human immunoglobulins 46,47,48

チトクローム c Cytochrome c 48

ミオグロビン分解物 Peptide fragments from myoglobin 48

グリシンオリゴマー Glycine oligomers 49

カゼイン Casein 50

タンパク分子量マーカー Proteins for molecular weight marker 50,51

有機酸、脂肪酸誘導体 Organic acids, Fatty acid derivatives

有機酸 Organic acids 52

ピルビン酸 Pyruvic acids 52

不飽和脂肪酸メチルエステル Unsaturated fatty acid methyl esters 53

リノレン酸メチル異性体 Methyl linolenate isomers 53

トリアシルグリセロール Triacylglycerols 53

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食品添加物 Food additives

食品保存料 Food preservatives 54

パラオキシ安息香酸エステル類 p-Hydroxybenzoic acid esters 55

EDTAキレート EDTA-chelates 55

酸化防止剤 Antioxidants 55,56,57

タール色素 Color additives 57

水溶性アナトー Annatto extract 58

市販炭酸飲料中のアスパルテーム Aspartame in a soft drink 58,59

ステビオサイドとレバウディオサイドA Stevioside and rebaudioside A 59

グリチルリチン酸 Glycyrrhizic acid 59

天然物 Natural products

アルカロイド Alkaloids 60

アルカロイド-酢酸緩衝液 Alkaloids -acetate buffer 60

プリンアルカロイド Purine alkaloids 61

生薬成分（配糖体） Crude drugs(Glycosides) 61

ペオニフロリン Paeoniflorin 62

当帰芍薬散中のペオニフロリン Paeoniflorin in Toki-shakuyaku-san 62

トリテルペン系サポニン Saponins from roots of rubus suavissimus S.Lee 62

サイコサポニン Saikosaponins 63

ジンセノサイド Ginsenosides 63,64

フラボノイド Flavonoids 64

クエルセチンとその配糖体 Quercetin and its glycosides 65

バイカリン Baicalin 65

フラバノン配糖体 Flavanone glycosides 65

市販みそ中のイソフラボン Isoflavones from miso, fermented soybean paste 66

カテキン類 Catechins 66

2-フェニルエチルグリコシド 2-Phenylethyl glycosides 67

センノシド Sennosides 67

アルブチンおよび関連化合物 Arbutin and related compounds 68

ペオノール Paeonol 68,69

アフラトキシン Aflatoxins 69

クロロフィル Chlorophyll a and b 69

トウガラシ中のカプサイシン類 Capsaicinoids from red papper 70

トウガラシ中のカプサイシン類 -LC/MS Capsaicinoids from red papper -LC/MS 70

ステロイド Steroids

ステロイド Steroids 71,74

副腎皮質ステロイド Adrenocorticosteroids 71,72,73,74

ヒドロコルチゾンとプレドニゾン Hydrocortisone and prednisone 72

男性ホルモン Androgens 75

テストステロン誘導体 Synthetic androgens 75,76

女性ホルモン Estrogens and progestins 76

エストラジオール誘導体 Estrogens 76,77

ノルゲストレルとエチニルエストラジオール Norgestrel and ethinylestradiol 77

豚胆中の胆汁酸のフェナシルエステル誘導体 Phenacyl esters of bile acids from pig gall 77

エクダイソン Ecdysones 78

ステロール Sterols 78

強心配糖体 Cardiac glycosides 79

センソ成分 Steroids from toad venom 80

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INDEX医薬品、生体関連物質 Drugs, Metabolites

風邪薬成分 Ingredients in a cough/cold medication 81,82

市販風邪薬 A commercial cough/cold medication 81,82

市販鎮痛解熱剤 Pain-killing drugs 83

インドメタシン Indometacin 83

解熱鎮痛薬成分 Analgesics 83

解熱鎮痛薬 Pain-killing drugs 84

コデイン Codeine 84

市販点鼻薬 A commercial nasal spray 84

鼻炎用点鼻剤 Collunarium 85

塩化ベンゼトニウム Benzethonium chloride 85

塩化ベンザルコニウム Benzalkonium chlorides 85

殺菌剤 Disinfectans 86

クロルヘキシジン Chlorhexidine 86

鼻炎薬成分 Ingredients in a drug for rhinitis 86

点眼剤成分 Ingredients in an eye drop 86

抗ヒスタミン薬 Histamine antagonists 87

酸性非ステロイド性抗炎症薬 Acidic non-steroidal anti-inflammatory drugs 87,88

酸性非ステロイド性解熱鎮痛抗炎症薬 -LC/MS Acidic non-steroidal anti-inflammatory drugs -LC/MS 88

三環系抗うつ薬 Tricyclic antidepressants 88,89

抗精神病薬 Antipsychotic drugs 89

抗てんかん薬 Antiepileptics 89

抗高脂血症剤 Hypolipidemic drugs 90

ロバスタチン Lovastatin 90

酸性および塩基性医薬品(pH6.8) Acidic and basic drugs(pH6.8) 91

酸性および塩基性医薬品(pH8.8) Acidic and basic drugs(pH8.8) 91

カルシウム拮抗薬 Calcium antagonists 91,92

不整脈治療薬 Antiarrhythmic drugs 92

局所麻酔薬 Local anesthetics 93

利尿薬 Diuretics 93

制がん剤および関連化合物 Antineoplastic and related compounds 94

葉酸および葉酸拮抗物質 Folic acid and its antagonists 94

抗HIVヌクレオシド誘導体 Anti-human immunodeficiency virus (HIV) agents 94,95

ニコチン酸構造類縁体 Nicotinic acid analogues 95

塩基性化合物 Basic compounds 96

殺菌薬 Disinfectants 96

アシクロビル Acyclovir 96

塩化カルニチン Carnitine chlorides 96

キサンチンオキシターゼ阻害剤および関連化合物 Xanthine oxidase inhibitor and related compounds 97

カフェイン代謝物 Caffeine metabolites 97

尿中のカフェイン代謝物 Caffeine metabolites in human urine 97

プリン代謝物 Purine catabolites 98

カテコールアミン Catecholamines 98

馬尿酸類 Hippuric and methylhippuric acids 98,99

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抗菌剤、抗生物質 Antibiotics

合成抗菌剤 Antibacterial agents 100

サルファ剤 Sulfonamides 100,101,102

抗生物質 Antibiotics 102

ナリジクス酸系抗生物質 Nalidixic acid antibiotics 102,103

セフェム系抗生物質 Cephalosporin antibiotics 103,104,105,106,107

セファロスポリンC Cephalosporin C 108

セフトリアキソン Ceftriaxone 108

セフォキシチンナトリウム Cefoxitin sodium 108,109

ペニシリン系抗生物質 Penicillin antibiotics 109

アモキシシリン Amoxicillin 109

抗生物質の高速分離 -LC/MS Rapid separation of antibiotics -LC/MS 110

エリスロマイシン Erythromycin antibiotic drugs 110

テトラサイクリン系抗生物質 Tetracycline antibiotics 110

バンコマイシン Vancomycin 110

農薬、環境関連物質 Pesticides, Environmental

ベンスリド Bensulide 111

チウラム Thiram 111

アシュラム Asulam 111

オキシン銅 Copper 8-quinolinolate 111

水質規制農薬9成分 Analysis of 9 pesticides 112

除草剤 Herbicides 112

アトラジン誘導体 Atrazine derivatives 112

トリアジン系除草剤 Triazine herbicides 112,113

尿素系除草剤 Urea herbicides 113

酸アミド系除草剤 Acid amide herbicides 113

カルバメート系除草剤 Carbamate herbicides 113

カルバメート系殺虫剤 Carbamate insecticides 114

防かび剤 Fungicides 114

イマザリル Imazalil 114

陰イオン界面活性剤 Anionic surfactants 115

市販洗濯洗剤中の陰イオン界面活性剤 Anionic surfactants in a commercial laundry detergent 115

市販クレンザー中の陰イオン界面活性剤 Anionic surfactants in a commercial cleanser 115

非イオン性界面活性剤 Nonionic surfactants 115,116

ポリエチレングリコール Polyethyleneglycols 116

アルデヒドおよびケトン類の2,4-DNPH誘導体 2,4-Dinitrophenylhydrazones of aldehydes and ketones 116

多環芳香族炭化水素 Polyaromatic hydrocarbons 117

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INDEXその他 The others

ヒドロキシ安息香酸 Hydroxybenzoic acids 118

レゾルシン酸異性体 Resorcylic acid isomers 118

安息香酸誘導体 Benzoic acid derivatives 118,119

メトキシ安息香酸異性体 Methoxybenzoic acid isomers 119

クロロ安息香酸異性体 Chlorobenzoic acid isomers 119,120

フタル酸異性体 Phthalic acid isomers 120

ポリフェノール Polyphenols 120

ジメチルフェノール異性体 Dimethylphenol isomers 121

フェノール誘導体 Phenol derivatives 121,122

クレゾール異性体 Cresol isomers 122

クロロフェノール異性体 Chlorophenol isomers 122

クロロアセトフェノン異性体 Chloroacetophenone isomers 123

ターフェニル異性体 Terphenyl isomers 123

フラーレン同族体 Fullerenes 123

1- (2-ピリジルアゾ) -2-ナフトール(PAN)金属キレート Metal-PAN chelates 124

配位性化合物 Coordination compounds 124

ヒノキチオール Hinokitiol 124

アミノアルコール Aminoalcohols 124

尿素及び関連化合物 Urea and related compounds 125

芳香族アミン Aromatic amines 125

光学異性体 Optical isomers

ビナフトール 1,1'-Bi-2-naphthol 126

トリフルオロアンスリルエタノール 2,2,2-Trifluoro-1-(9-anthryl)-ethanol 126

ヘキソバルビタール Hexobarbital 126,128

CBZ-フェニルアラニン CBZ-Phenylalanine(Z-Phe-OH) 126,129,130

塩酸プロプラノロール Propranolol hydrochloride 127,130

塩酸ケタミン Ketamine hydrochloride 127

クロルタリドン Chlorthalidone 127

フェノプロフェンカルシウム Fenoprofen calcium salt; hydrate 127

フルルビプロフェン Flurbiprofen 128,129

イブプロフェン Ibuprofen 128

インダパミド Indapamide 128

ケトプロフェン Ketoprofen 129

ナフチルエチルアルコール 1-(1-Naphthyl)-ethylalcohol 129

フェニルエチルアルコール Phenylethylalcohol 130

トレーガー塩基 Troger's base 130

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脂溶性ビタミンOil-soluble vitamins

1 ビタミンVitamins1

ビタミン

Column : YMC-Pack SIL (5μm, 12nm)150 × 4.6 mm I.D.

Eluent : cyclohexane/dichloromethane/acetic acid (100/0.5/0.5)Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 350 nm, 0.32 AUFSInjection : 5 μL (1.0 mg/mL)

(G910523T)

ビタミンＡ脂肪酸エステルVitamin A fatty acid ester

1. Retinol palmitate (Vitamin A palmitate)

Column : YMC-Pack Pro C18 (5μm, 12nm)150 × 4.6 mm I.D.

Eluent : methanol/water (92/8)Flow rate : 1.0 mL/minTemperature : 35 ℃Detection : UV at 325 nm, 0.02 AUFSInjection : 20 μL (0.5 μg/mL)

(A970708A)

レチノールRetinol

Retinol

1

Column : YMC-Pack ODS-A (5μm, 12nm)150 × 4.6 mm I.D.

Eluent : acetonitrile/methanol/THF (50/45/5)Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : VIS at 450 nm, 0.16 AUFSInjection : 5 μL (0.05 mg/mL)

(G910523Q)

カロテンCarotene

1. α-Carotene2. β-Carotene

1. Menadione (Vitamin K3)2. Retinol (Vitamin A)3. Retinol acetate4. Menaquinone (Vitamin K2)5. δ-Tocopherol6. Ergocalciferol (Vitamin D2)7. Cholecalciferol (Vitamin D3)8. α-Tocopherol (Vitamin E)9. α-Tocopherol acetate

10. Phylloquinone (Vitamin K1)


Eluent : acetonitrileFlow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 280 nm, 0.08 AUFSInjection : 15 μL (0.01～0.21 mg/mL)

(G910415B)

1 ビタミンVitamins

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1

Vitamins

Column : YMC-Pack CN (5μm, 12nm)250 × 4.6 mm I.D.

Eluent : acetonitrile/water/THF (35/35/30)Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : VIS at 450 nm, 0.32 AUFSInjection : 7 μL (0.125 mg/mL)

(M910529A)

β-カロテンβ-Carotene

1. β-Carotene

Column : YMC-Pack ODS-AL (5μm, 12nm)150 × 4.6 mm I.D.

Eluent : methanol/chloroform (96/4)Flow rate : 1.5 mL/minTemperature : 35 ℃Detection : VIS at 455 nm, 0.04 AUFSInjection : 20 μL (4.0 μg/mL)

(A970716K)

β-カロテンβ-Carotene

1. β-Carotene


Eluent : hexane/2-propanol (99.6/0.4)Flow rate : 1.6 mL/minTemperature : ambient (28 ℃)Detection : UV at 265 nm, 0.01 AUFSInjection : 100 μL (1.0 μg/mL)

(C970808E)

ビタミン DVitamin D

1

Ergocalciferol (Vitamin D2)


Eluent : hexane/2-propanol (99.6/0.4)Flow rate : 1.6 mL/minTemperature : ambient (28 ℃)Detection : UV at 265 nm, 0.01 AUFSInjection : 100 μL (1.0 μg/mL)

(C970808H)

ビタミン DVitamin D

1

Cholecalciferol (Vitamin D3)

10


ビタミン


Eluent : hexane/THF/acetic acid (97/3/0.25)Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 295 nm, 0.08 AUFSInjection : 10 μL (0.20～0.31 mg/mL)

(G910523S)

トコフェロールTocopherols

1. α-Tocopherol2. β-Tocopherol3. γ-Tocopherol4. δ-Tocopherol


Eluent : hexane/2-propanol/acetic acid (1000/6/5)Flow rate : 1.4 mL/minTemperature : 35 ℃Detection : FLS at Ex 298 nm, Em 325 nmInjection : 20 μL (5～20 μg/mL)

(S970702A)



Eluent : acetonitrile/water (95/5)Flow rate : 1.0 mL/minTemperature : 40 ℃Detection : UV at 265 nmInjection : 10 μL (0.01 mg/mL)

(P040818A)

ビタミンD2とビタミンD3Vitamin D2 and vitamin D3


Eluent : acetonitrile/water (95/5)Flow rate : 1.0 mL/minTemperature : 40 ℃Detection : UV at 265 nmInjection : 10 μL (0.01 mg/mL)

(P040819C)

ビタミンD2とビタミンD3Vitamin D2 and vitamin D3

1

2

3

4

α-Tocopherol

β-Tocopherol

γ-Tocopherol

δ-Tocopherol

1 ビタミンVitamins 1

Vitamins

11

Column : YMC-Pack Polyamine II250 × 4.6 mm I.D.

Eluent : n-hexane/tetrahydrofuran/acetic acid/water (93/7/0.4/0.01)Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 295 nm, 0.08 AUFSInjection : 7 μL (0.25 mg/mL)

(T910501A)


1. α-Tocopherol2. β-Tocopherol3. γ-Tocopherol4. δ-Tocopherol


Eluent : hexane/ethyl acetate (70/30)Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 295 nm, 0.08 AUFSInjection : 7 μL (0.25 mg/mL)

(T920728E)


1

2

3

4

α-Tocopherol

β-Tocopherol

γ-Tocopherol

δ-Tocopherol

Column : J'sphere ODS-M80 (4μm, 8nm)250 × 4.6 mm I.D.

Eluent : 2-propanol/water (68/32)Flow rate : 0.5 mL/minTemperature : 30 ℃Detection : UV at 295 nm, 0.032 AUFSInjection : 7 μL (0.25 mg/mL)

(S930726A)


δ-Tocopherol

γ-Tocopherol

β-Tocopherol

α-Tocopherol

1

2

3

4



Ref.) Y.Satomura, M. Kimura and Y. Itokawa, J.Chromatogr., 625, 372-376 (1992)

(S921217A)


δ-Tocopherol

γ-Tocopherol

β-Tocopherol

α-Tocopherol

1

2

3

4

12


ビタミン



Ref.) Y.Satomura, M. Kimura and Y. Itokawa, J.Chromatogr., 625, 372-376 (1992)

(S921216C)


δ-Tocopherol

γ-Tocopherol

β-Tocopherol

α-Tocopherol

1

2

3

4


Eluent : methanol/water (98/2)Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 284 nm, 0.51 AUFSInjection : 20 μL (1 mg/mL)

（日本薬局方第13改正記載条件）

(S970426E)

トコフェロールおよび酢酸トコフェロールTocopherol and tocopherol acetate

1

2

α-Tocopherol

α-Tocopherol acetate


Eluent : methanol/water (98/2)Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 284 nm, 0.51 AUFSInjection : 20 μL (1 mg/mL)


(S970426C)

トコフェロールおよび酢酸トコフェロールTocopherol and tocopherol acetate

1

2

α-Tocopherol

α-Tocopherol acetate


Eluent : methanol/ethanol (5/1)Flow rate : 0.8 mL/minTemperature : 40 ℃Detection : FLS at Ex 320 nm, Em 430 nm

reduction column (platinum black, 10 x 4.0 mmI.D.)Injection : 20 μL (100 ng/mL)

(A970728B)

ビタミン KVitamin K

1

2

Menaquinone (Vitamin K2)

Phylloquinone (Vitamin K1)

13


Vitamine


Eluent : A)20mM KH2PO4 - K2HPO4 (pH 6.6)B)20mM KH2PO4 - K2HPO4 (pH 6.6)/acetonitrile (80/20)0%B (0-5min), 0-100%B (5-20min, linear), 100%B (20-25min)

Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 254 nm, 0.32 AUFSInjection : 10 μL (0.04～0.31 mg/mL)

(G910415A)

水溶性ビタミンWater-soluble vitamins

1. L-Ascorbic acid (Vitamin C)2. Nicotinic acid3. Pyridoxine HCl (Vitamin B6)4. Nicotinamide5. Thiamine HCl (Vitamin B1)6. Folic acid7. Cyanocobalamin (Vitamin B12)8. Riboflavin (Vitamin B2)

Column : YMC-Pack ODS-AM (5μm, 12nm)150 × 4.6 mm I.D.

Eluent : 20mM NaH2PO4 - Na2HPO4 (pH 6.5)Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 210 nm, 0.08 AUFSInjection : 5 μL (0.02～0.58 mg/mL)

(G910523O)


1. Nicotinic acid2. Pantothenic acid (Na salt)3. Pyridoxine HCl (Vitamin B6)4. Nicotinamide5. Thiamine HCl (Vitamin B1)

1 2

3

4

5

6

10 20 300 min

1

2

3

4

5

6

10 20 300 min

Column : Hydrosphere C18 (5μm, 12nm)150 × 4.6 mm I.D.

Eluent : A)50mM KH2PO4-H3PO4(pH3.5)B)50mM KH2PO4-H3PO4(pH3.5)/acetonitrile (80/20)0-60%B(0-20min), 60%B(20-30min)

Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 210 nm, 0.32AUFSInjection : 5 μL (0.05 ～ 0.40 mg/mL)

(F001004B)


1. L-Ascorbic acid (Vitamin C)2. Pyridoxine HCl (Vitamin B6) 3. Nicotinamide4. Panthenol5. Caffeine6. Flavin mononucleotide sodium salt

(Vitamin B2)

Column : Hydrosphere C18 (5μm, 12nm)150× 4.6 mm I.D.


Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 210 nm, 0.32AUFSInjection : 5 μL Sample : 5 times dilution with 5% metaphosphoric acid

(F001004A)

市販ドリンク剤中の水溶性ビタミンA drink including vitamins

1. L-Ascorbic acid (Vitamin C)2. Pyridoxine HCl (Vitamin B6) 3. Nicotinamide4. Panthenol5. Caffeine6. Flavin mononucleotide sodium salt

(Vitamin B2)

14


ビタミン

1

2

3

4

5

10 20 300 min

1

2

3

4

5

10 20 300 min



Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 254 nm, 0.32AUFSInjection : 15 μL (0.04～0.60 mg/mL)

(F001011A)


1. Thiamine HCl (Vitamin B1)2. Pyridoxine HCl (Vitamin B6) 3. Nicotinamide4. Caffeine5. Flavin mononucleotide sodium salt

(Vitamin B2)



Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 254 nm, 0.32AUFSInjection : 15 μL Sample : 5 times dilution with water

(F001011B)

市販ドリンク剤中の水溶性ビタミンA drink including vitamins

1. Thiamine HCl (Vitamin B1)2. Pyridoxine HCl (Vitamin B6) 3. Nicotinamide4. Caffeine5. Flavin mononucleotide sodium salt

(Vitamin B2)

Column : YMC-Pack Ph (5μm, 12nm)150 × 4.6 mm I.D.

Eluent : acetonitrile/50mM NH4H2PO4 (10/90)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 210 nm, 0.16 AUFSInjection : 10 μL (0.02～0.30 mg/mL)

(M920212B)


1.p-Aminobenzoic acid (PABA)2.Biotin (Vitamin H)3.Riboflavin (Vitamin B2)4.Cyanocobalamin (Vitamin B12)



(M920212A)


1.p-Aminobenzoic acid (PABA)2.Biotin (Vitamin H)3.Cyanocobalamin (Vitamin B12)4.Riboflavin (Vitamin B2)


15

1

Vitamins

Column : YMC-Pack TMS (5μm, 12nm)150 × 4.6 mm I.D.


(M920212C)



Column : YMC-Pack C4 (5μm, 12nm)150 × 4.6 mm I.D.


(M920212D)




Eluent : 100mM CH3COOH/100mM CH3COONH4 (30/70, pH 5.1)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 254 nm, 0.16 AUFSInjection : 10 μL (0.04～0.20 mg/mL)

(M920207B)


1.L-Ascorbic acid (Vitamin C)2.Orotic acid (Vitamin B13)3.Pyrroloquinoline quinone (PQQ)4.Pyridoxine HCl (Vitamin B6)5.Nicotinamide

Column : YMC-Pack TMS (5μm, 12nm)150 × 4.6 mm I.D.

Eluent : 100mM CH3COOH/100mM CH3COONH4 (30/70, pH 5.1)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 254 nm, 0.16 AUFSInjection : 10 μL (0.04～0.20 mg/mL)

(M920207A)


1.L-Ascorbic acid (Vitamin C)2.Orotic acid (Vitamin B13)3.Pyrroloquinoline quinone (PQQ)4.Pyridoxine HCl (Vitamin B6)5.Nicotinamide

16


ビタミン



(T920525A)


1.Nicotinic acid2.Erythorbic acid3.L-Ascorbic acid

Column : YMC-Pack NH2 (5μm, 12nm)250 × 4.6 mm I.D.


(T920525B)


1.Nicotinic acid2.Erythorbic acid3.L-Ascorbic acid

Column : J'sphere ODS-H80 (4μm, 8nm)150 × 4.6 mm I.D.

Eluent : acetonitrile/50mM (NH4)2HPO4 (10/90)Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 254 nm, 0.13 AUFSInjection : 5 μL (0.04～0.48 mg/mL)

(S930712E)


1.Pyridoxine HCl2.Nicotinamide3.Benfotiamine4.Cyanocobalamin5.Riboflavin


Eluent : 20mM KH2PO4 - K2HPO4 (pH 6.9)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 254 nm, 0.13 AUFSInjection : 5 μL (0.05～0.2 mg/mL)

(S960912B)


1

2

3

4

Xanthine(I.S.)

Pyridoxal HCl

Pyridoxine HCl

Nicotinamide

1.

2.

3.

0 min5 10 15 20

1

32

45

6

4.

5.

6.

Pyridoxine HCl(Vitamin B6)

NCH3

CH2OHCH2OH

HO ・HCl

Nicotinic acid

Panthenol(Pantothenyl alcohol)

H2NCONH2

Urea

HOCH2CCH3

CH3

CONH(CH2)3OHCHOH

Allantoin

L-Ascorbic acid(Vitamin C)

N

COOH

O

O

OHHO

HCOHCH2OH

H2NCONH

NH

O

O

NH


17

1

Vitamins

1.

2.

3.

4.

5.

Pyridoxal HCl

Pyridoxine HCl

Nicotinamide

NCH3

CH2OHCH2OH

HO

NCH3

CH2OHCHO

HO

N

CONH2

・HCl

・HCl

N

COOH

Nicotinic acid

COOHH2N

2 4 60 min

1

2

3

4 5

-Aminobenzoic acidp

1.

2.

3.

4.

1

2

3 4

Allantoin

Pyridoxine HCl

Nicotinamide

NCH3

CH2OHCH2OH

HO

N

CONH2

・HCl

N

N O

O

H

H

H2NCONH

N

N

N

O

O

NH

CH2CH CH CHCH2OH

OH OH OH

H3C

H3CRiboflavin

0 5 10 15 20 min


Eluent : 10mM CH3COONH4


(S010125A)



Eluent : 50mM NH4H2PO4 - H3PO4 (pH 2.5) / acetonitrile (20/80) Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 210 nm, 0.064 AUFSInjection : 8 μL (0.01～0.04 mg/mL)

(S971121D)

アラントインおよび水溶性ビタミンAllantoin and water-soluble vitamins


Eluent : A)50mM (NH4)2HPO4

B)methanol/50mM (NH4)2HPO4 (60/40)10-100%B (0-15min, linear), 100%B (15-30min)


(S940202D)

ビタミンB1誘導体Thiamine and its modified compounds


Eluent : 50mM NH4H2PO4-H3PO4 (pH2.8)/acetonitrile (20/80)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 210 nm, 0.16 AUFSInjection : 10 μL (0.01～5.0 mg/mL)

尿素、アラントインおよび水溶性ビタミンUrea, allantoin and water-soluble vitamins

Thiamine HCl

Benfotiamine

Dicethiamine HCl

Bisbentiamine

1

2

3

4

(J030624B)

18


ビタミン

12 3

N

N

N

O

O

NH

CH2CH CH CH CH2OH

OH OH OH

H3C

H3C

Riboflavin

N

N

N

O

O

NH

CH2CH CH CH CH2O

OH OH OH

H3C

H3C

N

N

N

O

O

NH

CH2CH CH CH CH2O

OH OH OH

H3C

H3C

ONa

OH

P

O

O

P ONaO

O CH2O

OH

N

N N

N

NH2

OHP

O

ONa

Flavin sodium phosphate(Flavin mononucleotide sodium salt)

Flavin adenine dinucleotide disodium

1

2

3

10 200 min


Eluent : 50mM KH2PO4 - K2HPO4 (pH 5.9) / acetonitrile (90/10)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 260 nm, 0.32 AUFSInjection : 10 μL (0.1 mg/mL)

(S000803B)

リボフラビンおよび関連化合物Riboflavin and related compounds

5 10 15 20 250 min

1 2

3

4

5

6


Eluent : 20mM KH2PO4-K2HPO4(pH6.9)/acetonitrile (99/1) Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 260 nm, 0.13 AUFSInjection : 6 μL (0.05～0.10 mg/mL)

(F000707B)

ニコチン酸誘導体Nicotinic acid and derivatives


Eluent : acetonitrile/water (5/95)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 210 nm, 0.13 AUFSInjection : 5 μL (0.05～0.4 mg/mL)

(S961119B)

パントテニルアルコールPantothenyl alcohol

1

2

Pantothenyl alcohol

Resorcinol(I.S.)

1. Nicotinamide adenine dinucleotide phosphate oxidized form (NADP)

2. Nicotinic acid3. Nicotinamide adenine

dinucleotide phosphatereduced form (NADPH)

4. Nicotinamide5. β-Nicotinamide adenine

dinucleotide (NAD) 6. β-Nicotinamide adenine

dinucleotide reduced form (NADH)


Eluent : A)methanol/50mM KH2PO4 (10/90)B)methanol/50mM KH2PO4 (30/70)10%B (0-5min), 10-100%B (5-30min)

Flow rate : 0.7 mL/minTemperature : 37 ℃Detection : UV at 260 nm, 0.016 AUFSInjection : 10 μL (16.7 nmol/mL)

(S940630E)

補酵素A誘導体Coenzyme A and derivatives Malonyl-CoA

Coenzyme A glutathione disulfide

Coenzyme A

Methylmalonyl-CoA

Sccinyl-CoA

3-Hydroxy-3-methylglutaryl-CoA (HMG-CoA)

Acetoacetyl-CoA

Acetyl-CoA

Coenzyme A oxidized

Propionyl-CoA

1.

2.

3.

4.

5.

6.

7.

8.

9.

10.

Courtesy of Dr. Takamura, Ibaraki University

19


Vitamins


Eluent : acetonitrile/50mM NH4H2PO4 (70/30)Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 250 nm, 0.16 AUFSInjection : 10 μL (0.1 mg/mL)

(T910425A)

L-アスコルビン酸及びエリソルビン酸L-Ascorbic acid and erythorbic acid

1. Erythorbic acid2. L-Ascorbic acid (Vitamin C)


Eluent : 20mM CH3COONH4 in 5mM tetrabutylammonium bromideFlow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 250 nm, 0.08 AUFSInjection : 5 μL (0.05 mg/mL)

(G910523N)

L-アスコルビン酸及びエリソルビン酸L-Ascorbic acid and erythorbic acid

1. L-Ascorbic acid (Vitamin C)2. Erythorbic acid


Eluent : hexane/ethyl acetate/2-propanol/acetic acid (40/30/2/1)Flow rate : 1.5 mL/minTemperature : 35 ℃Detection : VIS at 540 nm, 0.01 AUFSInjection : 20 μL (10 μg/mL)

(C970808K)

L-アスコルビン酸の2,4-DNPH誘導体2,4-Dinitrophenylhydrazone of L-ascorbic acid

1. L-Ascorbic acid 2,4-DNPH

20

2 糖類Carbohydrates1

糖類


Eluent : ethanol/ethyl acetate/acetonitrile/water (30/40/5/25)Flow rate : 1.0 mL/minTemperature : 26 ℃Detection : RI, 32 x 10-6 RIU/FSInjection : 5 μL (14.3～28.6 mg/mL)

(T910723A)

単糖及び二糖類Monosaccharides and disaccharides

1.Fructose2.Glucose3.Sucrose4.Maltose5.Lactose



(T910718E)



Column : YMC-Pack Polyamine II250 × 4.6 mm I.D. × 2


(T910718D)

単糖類Monosaccharides

1.Rhamnose2.Fucose3.Xylose4.Fructose5.Mannose6.Glucose7.Galactose


Eluent : acetonitrile/water (75/25)Flow rate : 1.0 mL/minTemperature : 26 ℃Detection : RI, 32 x 10-6 RIU/FSInjection : 5 μL (14.3～28.6 mg/mL)

(T910726A)



2 糖類Carbohydrates

2

Carbohydrates

21


Eluent : A)0.01M NH4H2PO4 - (NH4)2HPO4(pH 6.7)B) 0.5M NH4H2PO4 - (NH4)2HPO4(pH 6.7)0%B (0-5min), 0-100%B (5-30min,linear), 100%B (30-40min)


(T910920A)

不飽和コンドロニ糖Unsaturated chondro-disaccharides

1.ΔDi-0S2.ΔDi-6S3.ΔDi-4S4.ΔDi-diSD

5.ΔDi-diSE

6.ΔDi-triS



(T910718B)

糖アルコールSugar alcohols

1. Glycerol2. Erythritol3. Xylitol4. Ribitol5. Arabitol6. Sorbitol7. Mannitol8. Maltitol9. Inositol

12

3

4

56

1. Glycerol2. Erythritol3. Xylitol4. Sorbitol5. Maltitol6. Lactitol

min0 10 20 30


Eluent : acetonitrile/water (75/25)Flow rate : 1.0 mL/minTemperature : 25 ℃Detection : RI, 32 × 10-6 RIU/FSInjection : 5 μL (5.0 mg/mL)

(F030603A)

糖アルコールSugar alcohols

1. Palatinose

2. Palatinit (*) (Isomalt)

1

2

10 200 min

(*)Mixture of 6-O-α-D-Glucopyranosyl-D-sorbitol and 1-O-α-D-Glucopyranosyl-D-mannitol


Eluent : acetonitrile/water (70/30)Flow rate : 1.0 mL/minTemperature : 25 ℃Detection : RI, 64 x 10-6 RIU/FSInjection : 15 μL (5.0 mg/mL)

(F030616B)

パラチノースとパラチニットPalatinose and Palatinit

22


2

糖　類


Eluent : acetonitrile/water (70/30)Flow rate : 1.0 mL/minTemperature : ambient (26 ℃)Detection : RI, 32 x 10-6 RIU/FSInjection : 20 μL (4.0 mg/mL)

(A970625G)

オリゴ糖Oligosaccharides

1.2.



(A970701G)


1.2.

1. Sucralose 2. Erythritol 3. Xylitol 4. Fructose 5. Sorbitol 6. Glucose 7. Sucrose 8. Palatinose 9. Maltitol10. Maltose11. Lactitol

116

10

5

2

3

7

4

8

9

1

0 10 20 30 40 min



(F030613C)

糖類および甘味料の一斉分析Sugars and sweeteners

F030617F

116 10

53

7

4

89

1

0 10 20 30

2

0 10 20 30F030618A

7

9

6543

2

10

1. Sucralose 2. Erythritol 3. Xylitol 4. Fructose 5. Sorbitol 6. Glucose 7. Sucrose 8. Palatinose 9. Maltitol10. Maltose11. Lactitol

A) Standard

B) Hard candy (Sample KM)

40 min

40 min


Eluent : acetonitrile/water (75/25)Flow rate : 1.0 mL/minTemperature : 25 ℃Detection : RI, 32 x 10-6 RIU/FSInjection : 5 μL Sample : A) 5 mg/mL

B) 50 mg/mL (50% ethanol solution of candy)

(F030618A)

市販キャンディー中の糖類Sugars in hard candy

23


2

Carbohydrates



(A970627F)


1.2.

Column : YMC-Pack Polyamine II 250 × 4.6 mm I.D.

Eluent : acetonitrile/water (70/30)Flow rate : 1.0 mL/minTemperature : ambient (27 ℃)Detection : RI, 32 x 10-6 RIU/FSInjection : 10 μLSample : 10 times dilution with water

(A970630C)

市販清涼飲料水のオリゴ糖Oligosaccharides of soft drink

1.2.



(A970628H)


1.

(Lactosyl fructoside)

1

2

3

4

10 20 300 min


Eluent : waterFlow rate : 1.0 mL/minTemperature : 30 ℃Detection : RI, 64 x 10-6 RIU/FSInjection : 5μL (10 mg/mL)

(F000725B)

フルクトオリゴ糖Fructooligosaccharides

1. Frufβ2→1αGlc (Sucrose)

2. Frufβ2→1Frufβ2→1αGlc (1-Kestose)

3. Frufβ2→[1Frufβ2]2→1αGlc (Nystose)

4. Frufβ2→[1Frufβ2]3→1αGlc (1-Fructofuranosyl-D-nystose)

24


2

糖　類

Column : YMC-Pack ODS-AQ (5μm, 12nm)250 × 10.0 mm I.D.

Eluent : waterFlow rate : 3.0 mL/minTemperature : ambient (25 ℃)Detection : RI

(Y910501A)

マルトオリゴ糖(G1～G7)Maltooligosaccharides(G1～～G7)

1.Glucose(G1)2.Maltose(G2)3.Maltotriose (G3)4.Maltotetraose (G4)5.Maltopentaose (G5)6.Maltohexaose (G6)7.Maltoheptaose (G7)

Courtesy of Dr.Kimura and Dr.Chiba, Hokkaido University, Japan

5 10 15 20 250 min

1

2

3

4

5

67


Eluent : waterFlow rate : 1.0 mL/minTemperature : ambient (26℃)Detection : RI, 64 x 10-6 RIU/FSInjection : 10 μL (5.0 mg/mL)

(F000612D)


1. Glucose (G1)2. Maltose (G2)3. Maltotriose (G3)4. Maltotetraose (G4)5. Maltopentaose (G5)6. Maltohexaose (G6)7. Maltoheptaose (G7)



(A970703A)


1.

(Lactosyl fructoside)

1. Glucose (G1)2. Maltose (G2)3. Maltotriose (G3)4. Maltotetraose (G4)5. Maltopentaose (G5)6. Maltohexaose (G6)7. Maltoheptaose (G7)

6

5

2

3

7

4

1

0 10 20 30 min


Eluent : acetonitrile/water (60/40)Flow rate : 1.0 mL/minTemperature : 25℃Detection : RI, 32 x 10-6 RIU/FSInjection : 10 μL (2.4 mg/mL)

(F030620A)


25


2

Carbohydrates


Eluent : acetonitrile/water (55/45)Flow rate : 1.0 mL/minTemperature : 26 ℃Detection : RI, 32 x 10-6 RIU/FSInjection : 200 μL (0.71～0.95 mg/mL)Sample : Manufactured by Nakano Vinegar Co., Ltd.,

Nakano Central Research Institute

(T910718A)


1.Maltoundecaose (G11)2.Maltododecaose (G12)3.Maltotridecaose (G13)4.Maltotetradecaose (G14)5.Maltopentadecaose (G15)


Eluent : waterFlow rate : 1.0 mL/minTemperature : 20 ℃Detection : RI, 8 x 10-6 RIU/FSInjection : 25 μL (1 mg/mL)Sample : Manufactured by Nakano Vinegar Co., Ltd.,


(M910604K)




Eluent : waterFlow rate : 1.0 mL/minTemperature : 20 ℃Detection : RI, 8 x 10-6 RIU/FSInjection : 25 μL (1 mg/mL)Sample : Manufactured by Nakano Vinegar Co., Ltd.,


(M910604L)




Eluent : methanol/water (5/995)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : RI, 8 x 10-6 RIU/FSInjection : 25 μL (1 mg/mL)Sample : Manufactured by Nakano Vinegar Co., Ltd.,


(M910604M)



26


2

糖　類


Eluent : methanol/water (5/995)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : RI, 8 x 10-6 RIU/FSInjection : 25 μL (1 mg/mL)Sample : Manufactured by Nakano Vinegar Co., Ltd.,


(M910604N)



Column : YMC-Pack Diol-60500 × 8.0 mm I.D.

Eluent : waterFlow rate : 1.0 mL/minTemperature : ambient (24 ℃)Detection : RI, 64 x 10-6 RIU/FSInjection : 60 μL (0.60～0.75 mg/mL)

(G911216E)


1.Maltopentadecaose (G15)2.Maltotridecaose (G13)3.Maltoundecaose (G11)4.Maltoheptaose (G7 )5.Maltopentaose (G5 )6.Maltotriose (G3 )7.Glucose (G1 )


Eluent : waterFlow rate : 1.0 mL/minTemperature : ambient (24 ℃)Detection : RI, 64 x 10-6 RIU/FSInjection : 60 μL (0.60～0.75 mg/mL)

(G911216F)


1.Maltopentadecaose (G15)2.Maltotridecaose (G13)3.Maltoundecaose (G11)4.Maltoheptaose (G7 )5.Maltopentaose (G5 )6.Maltotriose (G3 )7.Glucose (G1 ) 1. α-Cyclodextrin�

2. β-Cyclodextrin�3. γ-Cyclodextrin

1

23

10 200 min


Eluent : acetonitrile/water (60/40)Flow rate : 1.0 mL/minTemperature : 25℃Detection : RI, 32 x 10-6 RIU/FSInjection : 10 μL (0.4～0.5 mg/mL)

(F030617B)

サイクロデキストリンCyclodextrins

27


2

Carbohydrates


Eluent : methanol/water (7/93)Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : RI, 32 x 10-6 RIU/FSInjection : 8 μL (5～15 mg/mL)

(M910417B)


1.γ-Cyclodextrin2.α-Cyclodextrin3.β-Cyclodextrin



(M910417A)


1.γ-Cyclodextrin2.α-Cyclodextrin3.β-Cyclodextrin


Eluent : waterFlow rate : 1.0 mL/minTemperature : ambient (24 ℃)Detection : RI, 32 x 10-6 RIU/FSInjection : 30 μL (1.67 mg/mL)

(G911216I)


1.γ-Cyclodextrin2.β-Cyclodextrin3.α-Cyclodextrin


Eluent : A)waterB)methanol2%B (0-5min), 2-30%B (5-15min, linear),30-100%B (15-20min, linear), 100%B (20-30min)

Flow rate : 1.0 mL/minTemperature : 30 ℃Detection :Evaporative Mass Analyzer (ACS Ltd.)

Temp 150Time Constant 5PMT 3Attenuation 16Pressure 20 psi

Injection : 200 μL (1.5～15 mg/mL)

(M910423A)


1.γ-Cyclodextrin2.α-Cyclodextrin3.β-Cyclodextrin4.O-Dimethyl- β-cyclodextrin5.O-Trimethyl-β-cyclodextrin

28


2

糖　類



(M910417C)


1.O-Dimethyl- β-cyclodextrin2.O-Trimethyl-β-cyclodextrin

1

2

34

5

1. N-Acetylglucosamine2. N-Acetyl-chitobiose3. N-Acetyl-chitotriose4. N-Acetyl-chitotetraose5. N-Acetyl-chitopentaose

min0 5 10 15 20



(F030601A)

キチンオリゴ糖N-Acetyl-chitooligosaccharides


Eluent : methanol/20mM NH4H2PO4 (5/95)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : FLS at Ex.320 nm, Em. 400 nm, 8 mV/FSInjection : 2 μL (3.3 pmol/mL)Sample : PA-Sugar Chain Series, manufactured by TAKARA SHUZO CO., LTD.

(G910607C)

ピリジルアミノ化(PA)糖Pyridylamino(PA)-Sugar chains

1.PA-Sugar Chain 014





(G910607B)





29


2

Carbohydrates



(G910607A)






Eluent : acetonitrile/20mM NH4H2PO4 (60/40)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : FLS at Ex. 320 nm, Em. 400 nm, 8 mV/FSInjection : 5 μL (3.3 pmol/mL)Sample : PA-Sugar Chain Series, manufactured by TAKARA SHUZO CO., LTD.

(G910620B)







(G910620A)






Eluent : waterFlow rate : 1.0 mL/minTemperature : ambient (25 ℃)Detection : RI, 64 x 10-6 RIU/FSInjection : 40 μL (25 mg/mL)

(G911226A)

ポリデキストロースPolydextrose

1.Polydextrose II

30


Eluent : waterFlow rate : 1.0 mL/minTemperature : ambient (24 ℃)Detection : RI, 16 x 10-6 RIU/FSInjection : 180 μL (0.25 mg/mL)Sample : Shodex STANDARD P-82, manufactured by SHOWA DENKO K.K.

(G911216A)

プルランPullulans

1.Pullulan P-800 (MW 853,000)2.Pullulan P-50 (MW 48,000)3.Pullulan P-20 (MW 23,700)4.Pullulan P-5 (MW 5,800)



(G911216B)





(G911216C)




2

糖　類



(G911216D)



31


2

Carbohydrates



(G920107A)

コンドロイチンChondroitin

1.Chondroitin, Na salt



(G920107C)

コンドロイチン硫酸ＡChondroitin sulfate A

1.Chondroitin sulfate A, Na salt



(G920107E)

コンドロイチン硫酸ＢChondroitin sulfate B

1.Chondroitin sulfate B, Na salt



(G920107D)

コンドロイチン硫酸ＣChondroitin sulfate C

1.Chondroitin sulfate C, Na salt

32



(G920107B)

ヒアルロン酸Hyaluronic acid

1.Hyaluronic acid, Na salt



(G920107I)

ヘパリンHeparin

1.Heparin, Na salt



(G920107F)

ヘパラン硫酸Heparan sulfate

1.Heparan sulfate, Na salt


2

糖　類



(G920107G)

ケラタン硫酸Keratan sulfate

1.Keratan sulfate, Na salt

33


2

Carbohydrates



(G920107H)

ケラタンポリ硫酸Keratan polysulfate

1.Keratan polysulfate, Na salt

34

3 核酸Nucleic acids

3

核　酸


Eluent : A)20mM CH3COOH - CH3COONH4 (pH 3.5)B)20mM CH3COOH - CH3COONH4 (pH 3.5)/methanol (90/10)30%B (0-5min), 30-100%B (5-13min,linear), 100%B (13-40min)


(T910404C)

核酸塩基・ヌクレオシドNucleosides and bases

1.Cytocine2.Cytidine3.Uracil4.Deoxycytidine5.Guanine6.Adenine7.Hypoxanthine8.Uridine9.Xanthine

10.Thymine11.Inosine12.Guanosine13.Deoxyinosine14.Deoxyguanosine15.Xanthosine16.Thymidine17.Adenosine18.Deoxyadenosine


Eluent : A)20mM CH3COOH - CH3COONH4 (pH 3.5)B)20mM CH3COOH - CH3COONH4 (pH 3.5)/methanol (90/10)30%B (0-5min), 30-100%B (5-13min,linear), 100%B (13-40min)


(T910404A)

核酸塩基・ヌクレオシドNucleosides and bases

1.Cytocine2.Cytidine3.Uracil4.Deoxycytidine5.Guanine6.Adenine7.Hypoxanthine8.Uridine9.Xanthine

10.Thymine11.Inosine12.Guanosine13.Deoxyinosine14.Deoxyguanosine15.Xanthosine16.Thymidine17.Adenosine18.Deoxyadenosine

1

2

3

4

67

5

5 100 min

N

N

O

O

H

H

N

N

OH

NH2

N

N

O

O

H

H

CH3

N

NN

NO

HH

H2N

N

N

O

O

H

H

F

N

N

OH

NH2F

1.

Cytosine

2.

5-Fluorocytosine

3.

Uracil

4.

5-Fluorouracil

5.

Guanine

6.

Thymine

7.

Adenine

NH2

N

N

N

N

H



(J010209E)

核酸塩基Nucleic acid bases

1

2

3

4 5

1.

2.

3.

4.

5.

N

NO

OH

HUracil

N

N

OH

NH2

Cytosine

N

NO

OH

H

CH3

Thymine

Guanine

N

NN

NO

HH

H2N

Adenine

NH2

N

NN

N

H

0 10 20 min


Eluent : 20mM KH2PO4 - K2HPO4 (pH 6.9) Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 254 nm, 0.064 AUFSInjection : 8μL (0.01～0.02 mg/mL)

(S991029A)

核酸塩基Nucleic acid bases


3

Nucleic

acids

35


Eluen : A) 0.2M TEAA* (pH6.6)B) 0.2M TEAA (pH6.6)/acetonitrile (95/5)4%B (0-10min), 4-100%B (10-35min, linear), 100%B (35-50min)


* TEAA:triethylamine-acetic acid

(T910424A)

ヌクレオチドNucleotides

1. 5'-CMP2. 5'-UMP3. 5'-dCMP4. 5'-CDP5. 5'-GMP6. 5'-UDP7. 5'-IMP8. 5'-CTP9. 5'-UTP

10. 5'-GDP11. 5'-TMP12. 5'-AMP13. 5'-dGMP14. 5'-GTP15. 5'-TDP16. 5'-ADP17. 5'-TTP18. 5'-dAMP19. 5'-ATP

1

23

5 100 min

N�H

HN

O

O

Uracil

N�H

HN

O

O COOH

Orotic acid

N�H

HN

O

O COOH

F

5-Fluoroorotic acid

2.

3.

1.


Eluent : 20mM H3PO4

Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 254 nm, 0.256 AUFSInjection : 10μL (0.015～0.1 mg/mL)

(A000711A)

オロチン酸Orotic acid

2.

3.

4.

5.

1

2

34

6

7

8

9

10

11

5

1.

5 10 150 min

N

N

OH

O

OH

HOH2CO

NH2

Cytidine

N

N

OH

OHOH2CO

NH2

Deoxycytidine

N

HN

OH

O

OH

HOH2CO

O

Uridine

HN

N

N

N

O

OH

O

OH

HOH2C

Inosine

HN

N

N

N

O

OH

O

OH

HOH2CH2N

Guanosine

HN

N

N

N

O

OH

OHOH2C

Deoxyinosine

HN

N�H

N

N

O

OH

O

OH

HOH2C

O

Xanthosine

HN

N

N

N

O

OH

OHOH2CH2N

Deoxyguanosine

N

HN

OH

OHOH2CO

OCH3

Thymidine

N

N

N

N

OH

O

OH

HOH2C

NH2

Adenosine

N

N

N

N

OH

OHOH2C

NH2

Deoxyadenosine

6.

7.

8.

9.

10.

11.


Eluent : 20mM NH4H2PO4 - H3PO4 (pH 3.5) / methanol (92/8)Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 260 nm, 0.064 AUFSInjection : 15 μL (5～15 μg/mL)

(A000802B)

ヌクレオシドNucleosides

1

2

3

4

6

7

5

5 10 15 200 min

8

911

10



(A000922A)


1. 5'-UTP2. 5'-UDP3. 5'-CMP4. 5'-UMP5. 5'-dCMP6. 5'-GMP7. 5'-ATP8. 5'-IMP9. 5'-ADP

10. 5'-TMP11. 5'-AMP

36


3

核　酸


Eluent : 50mM KH2PO4 - H3PO4 (pH 3.5)Flow rate : 1.0 mL/minTemperature : 40 ℃Detection : UV at 260 nm, 0.16 AUFSInjection : 10 μL (0.05～0.1 mg/mL)

(T920525C)


1. 5'-CMP2. 5'-AMP3. 5'-UMP4. 5'-IMP5. 5'-GMP


Eluent : 50mM KH2PO4 - H3PO4 (pH 3.5)Flow rate : 1.0 mL/minTemperature : 40 ℃Detection : UV at 260 nm, 0.16 AUFSInjection : 10 μL (0.05～0.1 mg/mL)

(T920525D)


1. 5'-CMP2. 5'-AMP3. 5'-UMP4. 5'-IMP5. 5'-GMP

1

2

3

46

5

5 10 15 200 min


Eluent : 100mM KH2PO4

Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 260 nm, 0.256 AUFSInjection : 10μL (0.1～0.3 mg/mL)

(A000912B)


1. 5'-CMP2. 5'-UMP3. 5'-GMP4. 5'-IMP5. 5'-TMP6. 5'-AMP


Eluent : A)100mM triethylamine - acetic acid (pH 6.5)/acetonitrile (94/6)B)100mM triethylamine - acetic acid (pH 6.5)/acetonitrile (85/15)0-100%B (0-25min,linear)

Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 260 nm,0.01 AUFSInjection : 15 μL (2.0 pmol/μL )Sample : TAKARA ras Gene Probe set, manufacutured by TAKARA SHUZO CO., LTD.

(G910620C)

オリゴヌクレオチドOligonucleotides

1.c-Ha-ras/12 (20mer)GTGGGCGCC-AGC-GGTGTGGG

Ser2.c-Ha-ras/12 (20mer)

GTGGGCGCC-GCC-GGTGTGGGAla

3.c-Ha-ras/12 (20mer)GTGGGCGCC-GAC-GGTGTGGG

Asp4.c-Ha-ras/12 (20mer)

GTGGGCGCC-GTC-GGTGTGGGVal

5.c-Ha-ras/12 (20mer)GTGGGCGCC-CGC-GGTGTGGG

Arg

37


3

Nucleic

acids


Eluent : 20mM TEAA1) (pH 5.7)/acetonitrile (99/1)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 260 nm, 0.16 AUFSInjection : 5 μL (0.27～0.71 mg/mL)

1) TEAA: triethylamine-acetic acid

(T911206B)

糖ヌクレオチドSugar nucleotides

1.CDP-D-glucose2.UDP-D-glucose3.UDP-N-acetyl-D-glucosamine4.GDP-D-mannose5.ADP-D-glucose


Eluent : 100mM TEAA1) (pH 6.0)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 260 nm, 0.16 AUFSInjection : 5 μL (0.27～0.71 mg/mL)


(T911206E)




Eluent : A)0.1M phosophate buffer (pH 7)B)0.1M phosophate buffer (pH 7) / acetonitrile (75/25)20-27%B (0-15min), 27-35%B (15-90min)

Flow rate : 1.0 mL/minTemperature : ambientDetection : UV at 260 nmInjection : 10 μL Sample : Poly(A)

(Y920608A)

オリゴヌクレオチド，poly(A)Poly(A)


Eluent : 100mM TEAA1) (pH 6.0)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 260 nm, 0.16 AUFSInjection : 5 μL (0.27～0.71 mg/mL)


(T911206D)



Courtesy of Dr.Y.Baba, Kobe Women's College of Pharmacy


3

核　酸

38

Column : YMC-Pack Diol-300 + Diol-200500 × 8.0 mm I.D. × 2

Eluent : 0.1M KH2PO4 - K2HPO4 (pH 7.0) containing 0.2M NaClFlow rate : 0.7 mL/minTemperature : ambient (26 ℃)Detection : UV at 260 nm, 0.01 AUFSInjection : 3 μL (0.49 mg/mL)Sample : Plasmid pBR322 cleaved with restriction endonuclease MspⅠ

(G920109B)

プラスミドpBR322の制限酵素Msp I分解物Plasmid pBR322 restriction fragments

1.Plasmid pBR322 cleaved with restriction endonuclease MspⅠ

39

4 アミノ酸、ペプチド、タンパク質Amino acids, Peptides, Proteins

4

Aminoacids,Peptides,P

roteins

1

2

3

4

0 10 20 30 min


Eluent : 20mM KH2PO4


(A000419B)

アミノ酸Amino acids

1. L-Valine

2. L-Tyrosine

3. L-Phenylalanine

4. L-Tryptophan

1

10 200 min 10 200 min

1

(A) FLS (B) UV


Eluent : 10mM KH2PO4-K2HPO4(pH6.8)/acetonitrile (85/15)Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : (A)FLS at Ex 345 nm, Em 450 nm

(B)UV at 254 nm, 0.32 AUFSInjection : 15μLSample : OPA derivative

(F001115C)

市販ドリンク剤中のタウリンTaurine in nutritious drink

1. Taurine


Eluent : A) 60mM CH3COONa - CH3COOH (pH 6.6)/acetonitrile (94/6)B) 60mM CH3COONa - CH3COOH (pH 6.6)/acetonitrile (40/60)0-55%B (0-20min, linear), 100%B (20-25min), 0%B (25-35min)

Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 250 nm, 0.08 AUFSInjection : 5 μL (62.5 pmoL/μL )Sample : PTC derivatives

(G910523A)

PTC-アミノ酸PTC-amino acids

1. L-Aspartic acid2. L-Glutamic acid3. L-Serine4. Glycine5. L-Histidine6. L-Arginine7. L-Threonine8. L-Alanine9. L-Proline

10. NH3

11. L-Tyrosine12. L-Valine13. L-Methionine14. L-Cystine15. L-Isoleucine16. L-Leucine17. L-Phenylalanine18. L-Lysine


Eluent : A)acetonitrile/THF/300mM TEAA* (pH 5.1) (10/4/90)B)acetonitrile/THF/300mM TEAA (pH 5.1) (75/4/25)0-30%B (0-15min), 30-80%B (15-30min)

Flow rate : 0.2 mL/minTemperature : 50 ℃Detection : UV at 270 nm, 0.08 AUFSInjection : 30 μL (25pmol/10μL)

*TEAA: triethylamine-acetic acid

(K940331A)

PTH-アミノ酸PTH-amino acids

1. Asp2. His3. Arg4. Asn5. Glu6. Ser7. Gln8. Thr9. Gly

10. Ala11. Tyr12. Δ-Thr13. Pro14. Met15. Val16. Trp17. Phe18. Lys19. Ile20. Leu

10 20 30 40 50 600 min

1

2 3

4

6

7

89

10

11

12

13

14

15

16 17

18

19

5


Eluent : A)20mM KH2PO4-K2HPO4(pH6.5)B)20mM KH2PO4-K2HPO4(pH6.5)/acetonitrile (50/50)15-25%B(0-25min), 25-40%B(25-30min),40-65%B(30-50min),65-100%B(50-55min), 100%B(55-60min)

Flow rate : 1.0 mL/min(0-25min), 1.5 mL/min(25-60min)Temperature : 30 ℃Detection : FLS at Ex 470 nm, Em 530 nmInjection : 20 μLSample : NBD derivatives

(F001129E)

NBD-アミノ酸NBD-amino acids

1. L-Aspartic acid2. L-Glutamic acid3. L-Asparagine 4. L-Serine5. L-Histidine 6. Glycine7. L-Arginine 8. L-Threonine9. Taurine

10. L-Alanine11. L-Proline12. L-Valine13. L-Cystine14. L-Methionine15. L-Isoleucine16. L-Leucine17. L-Phenylalanine18. L-Lysine 19. L-Tyrosine

Column : YMC-Pack ODS/R (5μm, 12nm)250 × 4.6 mm I.D.

Eluent : A)0.01N HClB)acetonitrile0%B (0min),0%B (10min),50%B (60min,linear)

Flow rate : 1.0 mL/minDetection : UV 210 nmSample : Tryptic digests of synthetic pCDD 126

(Y890712B)

ペプチドマップPeptide mapping

Column : YMC-Pack ODS/R (5μm, 12nm)250 × 4.6 mm I.D.

Eluent : A)0.01N HClB)acetonitrile0%B (0min),0%B (10min),60%B (60min,linear)

Flow rate : 1.0 mL/minDetection : UV 210 nmSample : Tryptic digests of synthetic pCDD 88

(Y890712A)

ペプチドマップPeptide mapping

Courtesy of Dr.Nokihara, GBF-Gesellschaft für Biotechnologische Forschung Courtesy of Dr.Nokihara, GBF-Gesellschaft für Biotechnologische Forschung

Asn1 16 17 25

Pro Val Tyr Gly Ser Val Ser Asn Ala Asp Leu Met Asp Phe Lys Asn Leu Leu Asp His Leu Glu Asp Lys

Met26 39 50

Pro Leu Glu Asp Glu Ala Met Pro Pro Gln Val Leu Ser Glu Gln Asn Glu Glu Val Gly Ala Pro Leu Ser

Pro51 67 68 7574

Leu Leu Glu Val Pro Pro Trp Thr Gly Glu Val Asn Pro Ala Gln Arg Asp Gly Gly Ala Leu Gly Arg Gly

Pro76

T9T6 T7 T8 T10

91908988878382 92 98 99Trp Asp Ala Ser Asp Arg Ser Ala Leu Leu Lys Ser Lys Leu Arg Ala Leu Leu Ala Ala Pro Arg Ser Leu

Arg101 102 103

T14T11 T15

112 113109 110 125 126Arg Ser Ser Cys Phe Gly Gly Arg Met Asp Arg Ile Gly Ala Gln Ser Gly Leu Gly Cys Asn Ser Phe Arg Tyr

T2T1

T3

T4

T5

T13T12

39 50Ser Glu Gln Asn Glu Glu Val Gly Ala Pro Leu Ser

Pro51 67 68 7574

Leu Leu Glu Val Pro Pro Trp Thr Gly Glu Val Asn Pro Ala Gln Arg Asp Gly Gly Ala Leu Gly Arg Gly

Pro76

T9T6 T7 T8 T10

91908988878382 92 98 99Trp Asp Ala Ser Asp Arg Ser Ala Leu Leu Lys Ser Lys Leu Arg Ala Leu Leu Ala Ala Pro Arg Ser Leu

Arg101 102 103

T14T11 T15

112 113109 110 125 126Arg Ser Ser Cys Phe Gly Gly Arg Met Asp Arg Ile Gly Ala Gln Ser Gly Leu Gly Cys Asn Ser Phe Arg Tyr

T4T3'

T5

T13T12

40


4アミノ酸・ペプチド・タンパク質 Column : YMC-Pack ODS-A (5μm, 12nm)

150 × 4.6 mm I.D.Eluent : 0.1M sodium perchlorate in A

A)acetonitrile/water/TFA (5/95/0.05)Flow rate : 0.5 mL/minTemperature : 30 ℃Detection : UV at 220 nm,0.64 AUFSInjection : 10 μL

(H900710D)

酸化型および還元型グルタチオンGlutathione, oxidized and reduced form

1.Glutathione (GSH)2.Glutathione disulfide (GSSG)

41


4


roteins

Column : YMC-Pack PolymerC18250 × 4.6 mm I.D.

Eluent : acetonitrile/water/TFA (18/82/0.05)Flow rate : 0.5 mL/minTemperature : 30 ℃Detection : UV at 220 nm,0.08 AUFSInjection : 10 μL

(H900723B)

アンジオテンシンAngiotensins

1.Angiotensin III2.Angiotensin II3.Angiotensin I


Eluent : acetonitrile/20mM KH2PO4 (20/80)Flow rate : 0.5 mL/minTemperature : 30 ℃Detection : UV at 220 nm,0.08 AUFSInjection : 8 μL (0.05～0.15 mg/mL)

(G910520A)

アンジオテンシンAngiotensins

1.Angiotensin III2.Angiotensin II3.[Asn1,Val5] Angiotensin I4.[Val5] Angiotensin I5.Angiotensin I


Eluent : A)acetonitrile/water/TFA (30/70/0.1)B)acetonitrile/water/TFA (32/68/0.1)0%B (0-3min), 0-100%B (3-25min,linear), 100%B (25-40min)

Flow rate : 0.6 mL/minTemperature : 30 ℃Detection : UV at 220 nm, 0.16 AUFSInjection : 15 μL (0.2 mg/mL)

(G910522C)

インスリンInsulins

1.Insulin (Bovine)2.Insulin (Sheep)3.Insulin (Human)4.Insulin (Equine)5.Insulin (Porcine)



Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 220 nm,0.16 AUFSInjection : 15 μL (0.2 mg/mL)

(G910522B)






(G910522A)



3

4

651

2

0 5 10 15 20 min


Eluent : A) water / TFA (100/0.1)B) acetonitrile / TFA (100/0.1)20-40%B (0-20min, linear)


(A980827D)

ペプチドPeptides

1. Bradykinin2. Dynorphin A (Human,1-13)3. Eledoisin related peptide4. Neurotensin5. Bombesin6. Substance P


Eluent : A)acetonitrile/0.01N HCl (28/72)B)acetonitrile/0.01N HCl (35/65)0-100%B (0-60min,linear)


(G910522D)


1.Insulin (Bovine)2.Insulin (Sheep)3.Insulin (Human)4.Insulin (Porcine)

42


4アミノ酸・ペプチド・タンパク質

34

6

5

1

2

0 5 10 15 20 min


Eluent : A) water / TFA (100/0.1)B) acetonitrile / TFA (100/0.1)20-40%B (0-20min, linear)


(A980827C)


1. Oxytocin2. Met-Enkephalin3. Leu-Enkephalin4. Angiotensin I5. α-Mating factor6. Insulin

43


4


roteins


Eluent : A) water / TFA (100/0.05)B) acetonitrile / TFA (100/0.05)20-40%B (0-20min)

Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 220 nmInjection : 10 μL (0.075～0.25 mg/mL)

(N041021C)

ペプチド(MW 556 - 3, 465)Peptides(MW 556 - 3, 465)

1

23

4 6

7

5

0 5 10 15 20 min

Column : Hydrosphere C18 (5μm, 12nm)150× 4.6 mm I.D.

Eluent : A) water/TFA (100/0.1)B) acetonitrile/TFA (100/0.1)20-40%B(0-15 min, linear),40%B(15-20 min)


(A000308B)


5.00 10.00 15.00 20.00 min

5.00 10.00 15.00 20.00 min0.0

0.0

A) UV

B) MS

12

3

4

65

1

2 3

4

65

Column : YMC-Pack Pro C18 RS (5μm, 8nm)150× 2.0 mm I.D.

Eluent : A) water/TFA (100/0.01)B) acetonitrile/TFA (100/0.01)10-35%B(0-15 min),10%B(15-20 min)

Flow rate : 0.2 mL/minTemperature : 37 ℃Detection : A) UV at 220 nm, B) ESI positive-modeInjection : 4 μL (0.05 mg/mL)

(A020808A)

ペプチド - LC/MSPeptides - LC/MS

1. BAM-12P2. [D-Ala2,Met5]-Enkephalinamide3. α-Endorphin4. Met-Enkephalin5. [D-Ala2,Met5]-Enkephalin6. γ-Endorphin7. β-Endorphin(Human)

1

2

3

4 6

5

5 10 150 min

Column : YMC-Pack Pro C18 RS (5μm, 8nm)150× 4.6 mm I.D.

Eluent : A) water/TFA (100/0.1)B) acetonitrile/TFA (100/0.1)15-35%B(0-15 min)


(A010821A)


1. [Arg8]-Vasopressin2. Oxytocin3. Bradykinin4. Angiotensin II5. Leu-Enkephalin6. Angiotensin I

1. [Arg8]-Vasopressin2. Bradykinin3. Angiotensin II4. Oxytocin5. Angiotensin I6. Leu-Enkephalin

1. Oxytocin (MW 1,007)2. Met-Enkephalin (MW 574)3. Neurotensin (MW 1,673)4. Leu-Enkephalin (MW 556)5. γ-Endorphin (MW 1,859)6. β-Endorphin(Human) (MW 3,465)


Eluent : acetonitrile/water/TFA (20/80/0.1)Flow rate : 0.2 mL/minTemperature : 37 ℃Detection : UV at 220 nm, 0.08 AUFSInjection : 4 μL (34～65 μg/mL)

(O940804A)


1. Bradykinin2. Met-Enkephalin3. Angiotensin III4. Leu-Enkephalin



(O940804B)



44




Eluent : A)acetonitrile/water/TFA (5/95/0.1)B)acetonitrile/water/TFA (40/60/0.1)20-100%B (0-40min, linear)


(S930909D)


1.Met-Enkephalin2.Bradykinin3.Angiotensin II4.Leu-Enkephalin5.CCK-Octapeptide6.Insulin Chain B



(O940804D)



45


4


roteins


Eluent : A) water/TFA (100/0.1)B) acetonitrile/TFA (100/0.1)25-60%B (0-20 min)


(N040820A)

ペプチドおよびタンパク質(MW 4,330 - 17,000)Peptides and proteins(MW 4,330 - 17,000)



(O940804C)




Eluent : A)acetonitrile/water/TFA (20/80/0.05)B)acetonitrile/water/TFA (45/55/0.05)0-100%B (0-30min,linear)

Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 220 nm, 0.32 AUFSInjection : 30 μL

(H900606A)

ペプチドおよびタンパク質Peptides and proteins

1.Met-Enkephalin2.Leu-Enkephalin3.BSA4.α-Mating factor5.Insulin6.Ovalbumin


Eluent : A)acetonitrile/water/TFA (5/95/0.1)B)acetonitrile/water/TFA (60/40/0.1)30-90%B (0-20min, linear),90%B (20-25min)


(T931029A)

タンパク質Proteins

1.Ribonuclease A2.Cytochrome c3.Lysozyme4.Myoglobin

46




Eluent : A)acetonitrile/water/TFA (5/95/0.1)B)acetonitrile/water/TFA (60/40/0.1)30-90%B (0-20min, linear),90%B (20-25min)


(B931023A)

タンパク質Proteins

1.Ribonuclease A2.Cytochrome c3.Lysozyme4.Myoglobin


Eluent : A)water/TFA (100/0.1)B)acetonitrile/TFA (100/0.1)40-50%B (0-10min),50-90%B (10-15min),90%B (15-20min)


(N041126A)

タンパク質(MW 18,300 - 45,000)Proteins(MW 18,300 - 45,000)


Eluent : A)water/TFA (100/0.1)B)acetonitrile/2-propanol/TFA (50/50/0.1)30-75%B (0-15min),75%B (15-20min)


(N040930G)

タンパク質(MW 66,000 - 96,000)Proteins(MW 66,000 - 96,000)


Eluent : 0.1M NaH2PO4 - Na2HPO4 (pH 6.8) containing 0.1M Na2SO4

Flow rate : 0.7 mL/minTemperature : ambient (24 ℃)Detection : UV at 280 nm, 0.04 AUFSInjection : 60 μL (0.33 mg/mL)

(G920108G)

ヒト免疫グロブリンHuman immunoglobulins

1.Human IgM (κ),myeloma protein2.Normal human IgA3.Normal human IgG

1.β-Lactoglobulin B (Bovine milk) (MW 18,300)2.β-Lactoglobulin A (Bovine milk) (MW 18,400)3.α-Chymotrypsinogen A (Bovine pancreas) (MW 25,700)4.Ovalbumin (MW 45,000)

47


4


roteins




(G920108D)

ヒト免疫グロブリン(IgM κ鎖, ミエローマタンパク質)Human IgM(κ),myeloma protein

1.Normal human IgM (κ),myeloma protein




(G920108C)

ヒト免疫グロブリン(IgA)Normal human IgA

1.Normal human IgA




(G920108F)


1.Human IgM (κ),myeloma protein2.Normal human IgG




(G920108E)


1.Human IgM (κ),myeloma protein2.Normal human IgA




(G920108B)

ヒト免疫グロブリン(IgG λ鎖, ミエローマタンパク質)Human IgG(λ),myeloma protein

1.Human IgG (λ),myeloma protein




(G920108A)

ヒト免疫グロブリン(IgG)Normal human IgG

1.Normal human IgG


Eluent : 0.1M KH2PO4 - K2HPO4 (pH 7.0) containing 0.2M NaClFlow rate : 1.0 mL/minTemperature : ambient (24 ℃)Detection : UV at 215 nm, 0.08 AUFSInjection : 30 μL (0.2 mg/mL)

(G911217D)

チトクローム cCytochrome c

1.Cytochrome c (hexamer)2.Cytochrome c (tetramer)3.Cytochrome c (trimer)4.Cytochrome c (dimer)5.Cytochrome c (monomer)


Eluent : 0.1M KH2PO4 - K2HPO4 (pH 7.0) containing 0.2M NaCl/acetonitrile (70/30)Flow rate : 0.7 mL/minTemperature : ambient (25 ℃)Detection : UV at 215 nm, 0.32 AUFSInjection : 20 μL (2.0 mg/mL)Sample : cyanogen bromide cleavages of horse heart myoglobim.

Molecular Weight Marker for proteins,manufactured byFluka Chemie AG.

(G911218A)

ミオグロビン分解物Peptide fragments from myoglobin

1. 1-153 (MW 17,000)2. 1-131 (MW 14,500)3. 56-153 (MW 10,700)4. 56-131 (MW 8,200)5. 1-55 (MW 6,300)6. 132-153 (MW 2,500)

48



49


4


roteins


Eluent : 0.1M KH2PO4 - K2HPO4 (pH 7.0) containing 0.2M NaCl/acetonitrile (70/30)Flow rate : 0.7 mL/minTemperature : ambient (25 ℃)Detection : UV at 215 nm, 0.08 AUFSInjection : 25 μL (0.07～5.3 mg/mL)

(G911217C)


1.Insulin (Bovine)2.Neurotensin3.Angiotensin II4.Glycine


Eluent : 0.1M KH2PO4 - K2HPO4 (pH 7.0)/acetonitrile (70/30)Flow rate : 1.0 mL/minTemperature : ambient (24 ℃)Detection : UV at 215 nm, 0.08 AUFSInjection : 20 μL (0.25～2.5 mg/mL)

(G911227B)

グリシンオリゴマーGlycine oligomers

1.Pentaglycine2.Triglycine3.Glycine



(G911217A)



Column : YMC-Pack Diol-120500 × 8.0 mm I.D. × 2


(G911217B)




Eluent : 0.1M KH2PO4 - K2HPO4 (pH 7.0) containing 0.2M NaCl/acetonitrile (90/10)Flow rate : 1.0 mL/minTemperature : ambient (22 ℃)Detection : UV at 215 nm, 0.16 AUFSInjection : 15 μL (1.0 mg/mL)

(G920123B)

カゼインCasein

1.Casein


Eluent : 0.1M KH2PO4 - K2HPO4 (pH 7.0) containing 0.2M NaClFlow rate : 0.7 mL/minTemperature : ambient (26 ℃)Detection : UV at 280 nm, 0.08 AUFSInjection : 15 μL (100 μL/1 vial)Sample : MW-Marker(HPLC), manufactured by ORIENTAL YEAST CO.,LTD.

(G911213A)

タンパク分子量マーカーProteins for molecular weight marker

1.Glutamate dehydrogenase (MW 290,000)2.Lactate dehydrogenase (MW 142,000)3.Enolase (MW 67,000)4.Adenylate kinase (MW 32,000)5.Cytochrome c (MW 12,400)



(G911214A)





(G911214I)



50



51


4


roteins

Column : YMC-Pack Diol-200 + Diol-200500 × 8.0 mm I.D. + 300 × 8.0 mm I.D.


(G911214H)





(G911214B)


1.Glutamate dehydrogenase (MW 290,000)2.Lactate dehydrogenase (MW 142,000)3.Enolase (MW 67,000)4.Adenylate kinase (MW 32,000) 5.Cytochrome c (MW 12,400)



(G911214C)



52

5 有機酸、脂肪酸誘導体Organic acids, Fatty acid derivatives

5

有機酸・脂肪酸誘導体


Eluent : 20mM H3PO4 - NaH2PO4 (pH 2.8)Flow rate : 0.7 mL/minTemperature : 30 ℃Detection : UV at 220 nm, 0.08 AUFSInjection : 10 μL (0.007～1.8 mg/mL)

(T910226A)

有機酸Organic acids

1. Glycolic acid2. L-Malic acid3. Malonic acid4. Lactic acid5. Acetic acid6. Maleic acid7. Citric acid8. Fumaric acid9. Succinic acid10. Propionic acid 1

234

6

5

5 100 min


Eluent : 20mM NaH2PO4 - H3PO4(pH 2.8)Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 220 nm, 0.064 AUFSInjection : 10 μL (0.25～2.0 mg/mL)

(A001218A)


1. Tartaric acid2. L-Malic acid3. Lactic acid4. Acetic acid5. Citric acid6. Succinic acid


Eluent : 0.1% TFAFlow rate : 0.5 mL/minTemperature : 30 ℃Detection : UV at 220 nm, 0.08 AUFSInjection : 10 μL (0.016～2.2 mg/mL)

(Y910209)


1. L-Malic acid2. Lactic acid3. Acetic acid4. Maleic acid5. Succinic acid6. Propionic acid7. Fumaric acid


Eluent : 100mM H3PO4 (pH 1.7)Flow rate : 0.5 mL/minTemperature : 37 ℃Detection : UV at 220 nm, 0.64 AUFSInjection : 5 μL (0.7, 20 mg/mL)

(O940901H)

ピルビン酸Pyruvic acids

Phosphoenolpyruvic acidmonopotassium salt

Pyruvic acid

1

2

53

5 有機酸、脂肪酸誘導体Organic acids, Fatty acid derivatives

5

Organic

acids,Fatty

acidderivatives

Column : YMC-Pack Pro C18 RS (3μm, 8nm)150 × 4.6 mm I.D.

Eluent : acetonitrile/THF/water (35/30/35)Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 210 nmInjection : 2 μL (2.5 μL/mL)

(F040713A)

リノレン酸メチル異性体Methyl linolenate isomers


Eluent : A)acetonitrile/THF/water (10/30/60)B)acetonitrile/THF/water (65/30/5)20-100%B (0-40min, linear)

Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 210 nm, 0.51 AUFSInjection : 18 μL (0.25～4 μL/mL)

(S931012C)

不飽和脂肪酸メチルエステルUnsaturated fatty acid methyl esters

1.Methyl linolenate

2.Methylγ-linolenate

3.Methyl linoleate

4.Methyl oleate


Eluent : acetonitrile/2-propanol (55/45)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 210 nm, 0.08 AUFSInjection : 30 μL (0.08～1.66 mg/mL)

(O940914F)

トリアシルグリセロールTriacylglycerols

1. LLL2. LLO3. POL4. PPL5. OOO6. OOP7. PPO

L : linoleic acidO: oleic acidP: palmitic acid


Eluent : acetonitrile/water (81/19)Flow rate : 1.5 mL/minTemperature : ambientDetection : UV at 190 nm, 0.128 AUFSInjection : 20 μL (0.165 mg/mL)

(K930323A)

不飽和脂肪酸メチルエステルUnsaturated fatty acid methyl esters

1.Eicosapentaenoic acid

2.γ-Linolenic acid

3.Docosahexaenoic acid

4.Arachidonic acid

5.Linoleic acid

6.Eicosatrienoic acid

Courtesy of Dr. Yuji Saito, Tokyo Institute of Technology

1. Methyl linolenate2. Methyl γ-linolenate

54

6 食品添加物Food additives

6

食品添加物


Eluent : THF/water/TFA (20/80/0.1)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 230 nm, 0.16 AUFSInjection : 2 μL (0.13～0.32 mg/mL)

(O940819A)

食品保存料Food preservatives

1

2

3

4

Saccharin sodium

Dehydroacetic acid

Sorbic acid

Benzoic acid

1

23

5 10 150 min

COOH

CH3CH CHCH CHCOOH

O

O

O

COCH3

H3C


Eluent : 20mM CH3COONa-CH3COOH(pH 4.3) / acetonitrile (80/20)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 230 nm, 0.26 AUFSInjection : 10 μL (0.02 mg/mL)

(J011213F)



Eluent : THF/water/acetic acid (20/80/0.5)Flow rate : 2.0 mL/minTemperature : 37 ℃Detection : UV at 230 nm, 0.13 AUFSInjection : 5 μL (0.05 mg/mL)

(S970204F)


1.

2.

3.

Dehydroacetic acid

Sorbic acid

Benzoic acid


Eluent : THF/water/acetic acid (20/80/0.5)Flow rate : 2.0 mL/minTemperature : 37 ℃Detection : UV at 230 nm, 0.08 AUFSInjection : 4 μL (56 μg/mL)

(O940802I)


1

2

3

Dehydroacetic acid

Sorbic acid

Benzoic acid

1.

2.

3.

Benzoic acid

Sorbic acid

Dehydroacetic acid

55


6

Foodadditives

1

2

34

65

10 200 min

Methyl p-hydroxybenzoate

OH

COOCH3

Ethyl p-hydroxybenzoate

Propyl p-hydroxybenzoate

Butyl p-hydroxybenzoate

Isopropyl p-hydroxybenzoate

Isobutyl p-hydroxybenzoate

COOCHCH3

CH3

OH

OH

COOCH2CH3

COO(CH2)2CH3

OH

COOCH2CHCH3

CH3

OH

COO(CH2)3CH3

OH

1.

2.

3.

4.

5.

6.


Eluent : methanol/water (60/40)Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 270 nm, 0.32 AUFSInjection : 4 μL (0.1 mg/mL)

(J020826A)

パラオキシ安息香酸エステル類p-Hydroxybenzoic acid esters


Eluent : 50mM CH3COONa - CH3COOH (pH 4.6) containing20mM tetrabutylammonium bromide


(T940208A)

EDTAキレートEDTA-chelates

1

2

Fe (Ⅲ)-EDTA

Cu (Ⅱ)-EDTA

EDTA: Ethylenediamine-tetraacetic acid


Eluent : A)acetonitrile/methanol/5% acetic acid (20/20/60)B)acetonitrile/methanol/5% acetic acid (45/45/10)0-100%B (0-10min), 100%B (10-20min)


(A970524B)

酸化防止剤Antioxidants

1

2

3

4

5

6

7

4-Hydroxymethyl-2,6-di-tert-butylphenol(HMBP)

n-Octyl gallate(OG)

n-Dodecyl gallate(DG)

n-Propyl gallate(PG)

2,4,5-Trihydroxybutyrophenone(THBP)

tert-Butylhydroquinone(TBHQ)

Nordihydroguaiaretic acid(NDGA)


Eluent : A)acetonitrile/methanol/5% acetic acid (20/20/60)B)acetonitrile/methanol/5% acetic acid (45/45/10)0-100%B (0-5min), 100%B (5-15min)


(A970526C)


1

2

3

4


n-Octyl gallate(OG)

n-Dodecyl gallate(DG)





5

6

7

1

2

3

4 65

1.

2.

3.

4.

6.

5.

5 10 150 min

CH2CH CHCH2

CH3 CH3OH

OH

HO

HO

Nordihydroguaiaretic acid (NDGA)

OHC(CH3)3(CH3)3C

CH2OH

COOC8H17

HO

HO

HO

COOC12H25

HO

HO

HO

-Dodecyl gallate(DG)

OH

C(CH3)3

OCH3

OHC(CH3)3(CH3)3C

CH3

�

3(2)- -Butyl-4-hydroxyanisol � (BHA)

tert

4-Hydroxymethyl-2,6-� di- -butylphenol (HMBP)tert

2,6-Di- -butyl-� 4-methylphenol (BHT)

tert

-Octyl gallate(OG)n

n


Eluent : acetonitrile / methanol / 5% acetic acid (35/35/30)Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 280 nm, 0.32 AUFSInjection : 10 μL (0.05 ～ 0.5 mg/mL)

(J010215B)


56


6

食品添加物


Eluent : acetonitrile/methanol/5% acetic acid (20/20/60)Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 280 nm, 0.32 AUFSInjection : 10 μL (0.05～0.2 mg/mL)

(A970522B)


1

2

3



(A970522D)


1

2

3



(A970521B)


1

2

3









n-Octyl gallate(OG)

57


6

Foodadditives



(O940810D)


1

2

Butyl hydroxyanisol (BHA)

Dibutyl hydroxytoluene (BHT)


Eluent : A) 10mM NH4H2PO4 - (NH4)2HPO4 (pH 6.0)/methanol (90/10)B) 10mM NH4H2PO4 - (NH4)2HPO4 (pH 6.0)/methanol (20/80)0-100%B (0-40min, linear), 100%B (40-60min)

Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 254 nm, 0.32 AUFSInjection : 100 μL (5 μg/mL)

(M910427A)

タール色素Color additives 1. Tartrazine

(FD&C Yellow No.5, C.I.19140, Y-4)2. Amaranth

(C.I.16185, R-2)3. Indigo Carmine

(FD&C Blue No.2, C.I.73015, B-2)4. New Coccine

(C.I.16255, R-102)5. Sunset Yellow FCF

(FD&C Yellow No.6, C.I.15985, Y-5)6. Fast Green FCF

(FD&C Green No.3, C.I.42053, G-3)7. Brilliant Blue FCF

(FD&C Blue No.1, C.I.42090, B-1)8. Erythrosine

(FD&C Red No.3, C.I.45430, R-3)9. Acid Red

(C.I.45100, R-106)10. Phloxine

(C.I.45410, R-104)11. Rose Bengale

(C.I.45440, R-105)


Eluent : acetonitrile/10mM CH3COONH4 (25/75)Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 254 nm, 0.08 AUFSInjection : 10 μL (35～140 μg/mL)

(M910430C)

タール色素Color additives

1. Fast Green FCF(FD&C Green NO.3, C.I.42053, G-3)

2. Brilliant Blue FCF(FD&C Blue No.1, C.I.42090, B-1)

3. Erythrosine(FD&C Red No.3, C.I.45430, R-3)

4. Acid Red(C.I.45100, R-106)

5. Phloxine(C.I.45410, R-104)

6. Rose Bengale(C.I.45440, R-105)

Column :YMC-Pack ODS-A (5μm, 12nm)150 × 4.6 mm I.D.

Eluent : acetonitrile/10mM CH3COONH4 (8/92)Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 254 nm, 0.16 AUFSInjection : 5 μL (25～200 μg/mL)

(M910430A)

タール色素Color additives

1. Tartrazine(FD&C Yellow No.5, C.I.19140,Y-4)

2. Amaranth(C.I.16185, R-2)

3. Indigo Carmine(FD&C Blue No.2, C.I.73015, B-2)

4. New Coccine(C.I.16255, R-102)

5. Sunset Yellow FCF(FD&C Yellow No.6, C.I.15985, Y-5)


Eluent : acetonitrile/water/TFA (15/85/0.05)Flow rate : 0.5 mL/minTemperature : 30 ℃Detection : UV at 220 nm, 0.64 AUFSInjection : 5 μL

(H900710B)

市販炭酸飲料中のアスパルテームAspartame in a soft drink

1.Caffeine2.Aspartame3.Benzoic acid

58


6

食品添加物


Eluent : acetonitrile/water/acetic acid (65/34/1)Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : VIS at 454 nm, 0.16 AUFSInjection : 5 μL (2 mg/mL)Sample : Annatto pigment

(M910430E)

水溶性アナトーAnnatto extract

1. Annatto pigment


Eluent : 50mM KH2PO4/acetonitrile/methanol (84/3/13)Flow rate : 1.5 mL/minTemperature : 30 ℃Detection : UV at 214 nm, 0.64 AUFSInjection : 10 μL

(T910123)


1. Benzoic acid2. Caffeine3. Aspartame



(H900710A)



59


6

Foodadditives



(H900710C)




Eluent : acetonitrile/water (35/65)Flow rate : 0.5 mL/minTemperature : 30 ℃Detection : UV at 205 nm, 0.32 AUFSInjection : 7 μL

(G900727B)

ステビオサイドとレバウディオサイドAStevioside and rebaudioside A

1.Stevioside2.Rebaudioside A


Eluent : acetonitrile/water/5M acetic acid (400/560/40)Flow rate : 0.5 mL/minTemperature : 20 ℃Detection : UV at 254 nmInjection : 20 μL (0.05～0.25 mg/mL)


(F040622C)

グリチルリチン酸Glycyrrhizic acid

1

2

3

60

7 天然物Natural products

7

天然物


Eluent : acetonitrile/20mM KH2PO4(20/80)Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 210 nm, 0.16 AUFSInjection : 5 μL (0.05～2.19 mg/mL)

(G910523J)

アルカロイドAlkaloids

1.Hordenine sulfate2.Pilocarpine chloride3.Scopolamine bromide4.Atropine sulfate5.Cinchonine6.Sparteine sulfate7.Quinine8.Papaverine chloride9.Narcotine chloride


Eluent : A)acetonitrile/20mM KH2PO4 (17/83)B)acetonitrile/20mM KH2PO4 (40/60)0-100%B (0-30min, linear), 100%B (30-40min)


(G910523K)


1.Hordenine sulfate2.Pilocarpine chloride3.Scopolamine bromide4.Atropine sulfate5.Cinchonine6.Sparteine sulfate7.Quinine8.Papaverine chloride9.Narcotine chloride

1

2

3

10 200 min

4

6

5

1.

2.

3.

CinchonineN

CH2 CH

CHO N

H

H

H

Cinchonidine

HCH2 CH

CHO N

HH

N

Quinidine

Quinine

N

CH2 CH

CHO N

H

H

HCH3O

HCH2 CH

CHO N

HH

N

CH3O

Dihydroquinine

Dihydroquinidine

H

N

CH3CH2

CHO N

HH

CH3O

H

CHO N

HH

N

CH3O

CH3CH2

4.

5.

6.


Eluent : methanol/20mM KH2PO4 (35/65)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 235 nm, 0.08 AUFSInjection : 10 μL (0.01～0.03 mg/mL)

(J020828B)


1.

2.

3.

CinchonineN

CH2 CH

CHO N

H

H

H

Cinchonidine

HCH2 CH

CHO N

HH

N

Quinidine

Quinine

N

CH2 CH

CHO N

H

H

HCH3O

HCH2 CH

CHO N

HH

N

CH3O

Dihydroquinine

Dihydroquinidine

H

N

CH3CH2

CHO N

HH

CH3O

H

CHO N

HH

N

CH3O

CH3CH2

4.

5.

6.

1

2

3

10 200 min

4

6

5


Eluent : 20mM CH3COOH-CH3COONH4(pH4.9)/methanol (65/35)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 235 nm, 0.08 AUFSInjection : 10 μL (0.01～0.03 mg/mL)

(J021010D)

アルカロイド - 酢酸緩衝液Alkaloids - acetate buffer

61


7

NaturalP

roducts



(M910612C)

プリンアルカロイドPurine alkaloids

1. Trigonerlline2. Theobromine3. Theophylline4. Caffeine5. Phenol


Eluent : A)methanol/20mM NH4H2PO4 (5/95)B)methanol/20mM NH4H2PO4 (80/20)0-100%B (0-20min, linear), 100%B (20-40min)


(G910618B)

生薬成分（配糖体）Crude drugs(Glycosides)

1.Arbutin2.Salicin3.Esculine4.G-Strophanthin (Ouabain)5.Baicalin6.Barbaloin7.Glycyrrhizin8.Digitoxin




(G910607D)






(G910607E)



12

3

0 5 10 15 20 min


Eluent : acetonitrile/water/acetic acid (20/80/1)Flow rate : 0.4 mL/minTemperature : 25 ℃Detection : UV at 230 nm, 0.16 AUFSInjection : 10 μL (48～56 μg/mL)

(A980818A)

ペオニフロリンPaeoniflorin


Eluent : THF/50mM (NH4)2HPO4 (10/90)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 230 nm, 0.16 AUFSInjection : 3 μL (0.67, 0.33 mg/mL)

(O941121M)

ペオニフロリンPaeoniflorin

1

2

Paeoniflorin

p-Hydroxyacetophenone(I.S.)

1. Albiflorin2. Paeoniflorin3. p-Hydroxyacetophenone(I.S.)

62


7

天然物

Column : YMC-Pack PolymerC18 and YMC-Pack ODS-AM (5μm, 12nm)150 × 4.6 mm I.D.

Eluent : methanol/water (45/55) for PolymerC18methanol/water (50/50) for ODS-AM

Flow rate : 0.8 mL/minTemperature : 50 ℃Detection : UV at 210 nm, 0.32 AUFSSample : 1) β-D-Glucopyranosyl 2α,3β,23-trihydroxyurs-

12,18-dien-28-oate2) β-D-Glucopyranosyl 2α,3β,23-trihydroxyurs-

12,19-dine-28-oate

(Y910123)

トリテルペン系サポニンSaponins from roots of rubus suavissimus S. Lee

1

2

Courtesy of Dr.Ohtani,Hiroshima University,Japan


Eluent : THF/50mM (NH4)2HPO4 (10/90)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 230 nm, 0.16 AUFSInjection : 3 μL Sample : water extract from Toki-shakuyaku-san

(O941121I)

当帰芍薬散中のペオニフロリンPaeoniflorin in Toki-shakuyaku-san

1

Paeoniflorin

63


7

NaturalP

roducts



(S931210C)

サイコサポニンSaikosaponins

1

2

3

4

Column : J'sphere ODS-L80 (4μm, 8nm)150 × 4.6 mm I.D.


(S931210D)

サイコサポニンSaikosaponins

1

2

3

4

Saikosaponin c

Saikosaponin a

Saikosaponin b2

Saikosaponin d

Saikosaponin c

Saikosaponin a

Saikosaponin b2

Saikosaponin d


Eluent : acetonitrile/water (30/70)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 203 nm, 0.16 AUFSInjection : 10 μL (0.125, 0.5 mg/mL)

(A971120C)

ジンセノサイドGinsenosides

1

2

Ginsenoside-Rg1

Ginsenoside-Rb1



(A971118B)


1

2

Ginsenoside-Rg1

Ginsenoside-Rb1


Eluent : A)acetonitrile/water (20/80)B)acetonitrile/water (40/60)0-100%B (0-10min), 100%B (10-20min)

Flow rate : 0.4 mL/minTemperature : 37 ℃Detection : UV at 203 nm, 0.32 AUFSInjection : 4 μL (0.125, 0.5 mg/mL)

(A971121D)


1

2

Ginsenoside-Rg1

Ginsenoside-Rb1


Eluent : acetonitrile/10mM H3PO4 (30/70)Flow rate : 1.0 mL/minTemperature : 40 ℃Detection : UV at 260 nm, 0.064 AUFSInjection : 2 μL (0.05 mg/mL)

(D960722A)

フラボノイドFlavonoids

1

2

3

Myricetin

Quercetin

Kaempferol

64


7

天然物



(S961107C)

フラボノイドFlavonoids 1

2

3

4

5

6

Luteolin

Apigenin

Chrysoeriol

Baicalein

Chrysin

Acacetin



(S961111B)

フラボノイドFlavonoids

1

2

3

4

5

6

Luteolin

Apigenin

Chrysoeriol

Baicalein

Chrysin

Acacetin

65


7

NaturalP

roducts


Eluent : acetonitrile/10mM H3PO4 (30/70)Flow rate : 0.7 mL/minTemperature : 37 ℃Detection : UV at 280 nm, 0.064 AUFSInjection : 10 μL (0.01, 0.02 mg/mL)

（日本薬局方第13改正参照）

(S961105C)

バイカリンBaicalin

1

2



(A970430A)

フラバノン配糖体Flavanone glycosides

1.

2.

3.

4.

Baicalin


1

2

3

4

O

O

HO

OH

HO

OR

HO

1. Rutin R=rutinose

2. Isoquercitrin R=glucose

3. Quercitrin R=rhamnose

4. Quercetin R=H

5 10 150 min



(J020828F)

クエルセチンとその配糖体Quercetin and its glycosides

Narirutin

Naringin

Hesperidin

Neohesperidin



(A970506A)

フラバノン配糖体Flavanone glycosides

1.

2.

3.

4.

Narirutin

Naringin

Hesperidin

Neohesperidin


Eluent : 20mM KH2PO4 - H3PO4 (pH 2.4)/methanol (75/25)Flow rate : 0.8 mL/minTemperature : 37 ℃Detection : UV at 280 nm, 0.13 AUFSInjection : 8 μL (0.1～0.4 mg/mL)

(S940124A)

カテキン類Catechins

1

2

3

4

5

(-)-Epigallocatechin

(+)-Catechin

(-)-Epigallocatehin gallate

(-)-Epicatechin

(-)-Epicatechin gallate

1

23

4

5

1 ��

2

3

4

5


(+)-Catechin

(-)-Epicatechin

(-)-Epigallocatechin gallate


OH

OHO

OH

OH

OH

OH

OH

OHO

OHOH

OH

OH

OHO

OHOH

OH

OH

OHO

OH

OH

OH

O��CO OH

OH

OH

OH

OHO

OHOH

O��CO OH

OH

OH

10 200 min


Eluent : acetonitrile/ethyl acetate/water/TFA (9/3/88/0.1)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 280 nm, 0.32 AUFSInjection : 7 μL (0.2～0.8 mg/mL)

(F000828A)


1

2

34

10 20 30 400 min

Daidzin��

Genistin

Daidzein

Genistein

OH

O

O

O

OH

O

O

OO

OH

HOH2C

HOHO OH

OH

O

O

HO

OH

O

O

HO

OH

1

2

3

4

OOH

HOH2C

HOHO


Eluent : A)water/acetic acid (100/1)B)acetonitrile/water/acetic acid (50/50/1)30%B(0-5min), 30-70%B(5-35min)

Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 254 nm, 0.08AUFSInjection : 7 μLSample : methanol extract of miso

(F001025B)

市販みそ中のイソフラボンIsoflavones from miso,fermented soybean paste

66


7

天然物


Eluent : 20mM KH2PO4 - H3PO4 (pH 2.4)/methanol (75/25)Flow rate : 0.8 mL/minTemperature : 37 ℃Detection : UV at 280 nm, 0.13 AUFSInjection : 8 μL (0.1～0.4 mg/mL)

(S940122D)


1

2

3

4

5


(+)-Catechin

(-)-Epigallocatehin gallate

(-)-Epicatechin


67


7

NaturalP

roducts

12

3

4 5

2-Phenylethyl β-gentiobioside

2-Phenylethyl β-lactoside

2-Phenylethyl β-cellobioside

2-Phenylethyl β-maltoside

2-Phenylethyl β-melibioside

O

OH

HOHO

O

HO

O

OH

HOHO

O

O

OH

OH

HO

HO

HO

O

OH

OHO

O

O

OH

HOHO

HOHO

O

OH

OHO

O

�

O

OH

HOHO

HO

HOO

OH

OHO

O

O

OH

OH

HO

HO�

HO

O

OH

O

HOO

10 20 30 40 500 min

1

2

3

4

5

Courtesy of Dr.K.Sakata, Institute for Chemical Research Kyoto University, Japan


Eluent : acetonitrile/methanol/water (8/4/88)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 210 nm, 0.064 AUFSInjection : 5 μL (0.2 mg/mL)

(F000911A)

2-フェニルエチルグリコシド2-Phenylethyl glycosides


Eluent : methanol/water/acetic acid (30/65/5)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 340 nm, 0.16 AUFSInjection : 10 μL (0.1 mg/mL)

(A961126C)

センノシドSennosides

1

2

Sennoside B

Sennoside A



(A961126B)


1

2

Sennoside B

Sennoside A



(A961126D)


1

2

Sennoside B

Sennoside A


Eluent : methanol/10mM H3PO4 (5/95)Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 280 nm, 0.32 AUFSInjection : 5 μL (0.2～0.5 mg/mL)

(A970514D)

アルブチンおよび関連化合物Arbutin and related compounds

1

2

3



(A970514B)


1

2

3

1.

2.

3.

1

2

3

OH

O glucose

OH

OH

Arbutin

Hydroquinone

COOH

OHOHHO

Gallic acid

0 5 10 min

Column : YMC-PacK Pro C18 (3μm, 12nm)75 × 2.0 mm I.D.

Eluent : methanol / water / TFA (5/95/0.05)Flow rate : 0.2 mL/minTemperature : 30 ℃Detection : UV at 280 nm, 0.16 AUFSInjection : 1 μL (0.2～0.5 mg/mL)

(A980831L)


Arbutin

Hydroquinone

Gallic acid

Arbutin

Hydroquinone

Gallic acid

68


7

天然物


Eluent : acetonitrile/water/acetic acid (35/65/2)Flow rate : 1.0 mL/minTemperature : ambient (22 ℃)Detection : UV at 274 nm, 0.26 AUFSInjection : 10 μL (0.04, 0.2 mg/mL)


(S971106D)

ペオノールPaeonol

1

2

Paeonol


69


7

NaturalP

roducts


Eluent : acetonitrile/water/acetic acid (35/65/2)Flow rate : 1.0 mL/minTemperature : ambient (22 ℃)Detection : UV at 274 nm, 0.26 AUFSInjection : 10 μL (0.04, 0.2 mg/mL)


(S971107A)

ペオノールPaeonol

1

2

Paeonol



Eluent : methanolFlow rate : 1.0 mL/minTemperature : ambientDetection : VIS at 450 nm, 1 AUFSInjection : 100 μL Sample : extract of Spinacia with acetone/water (90/10)

(K940228A)

クロロフィルChlorophyll a and b

Chlorophyll b

Chlorophyll a

Courtesy of Dr. Toyoaki Aoki, University of Osaka Pref.

1

2


Eluent : methanol/water (40/60)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 365 nm, 0.04 AUFSInjection : 10 μL (7～10 ppm)

(K931124B)

アフラトキシンAflatoxins

1

2

3

4

Aflatoxin G2

Aflatoxin G1

Aflatoxin B2

Aflatoxin B1


Eluent : methanol/water (40/60)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 365 nm, 0.04 AUFSInjection : 10 μL (7～10 ppm)

(K931125A)

アフラトキシンAflatoxins

1

2

3

4

Aflatoxin G2

Aflatoxin G1

Aflatoxin B2

Aflatoxin B1

70


7

天然物

2.

3.

Capsaicin

Dihydrocapsaicin

min0 2 4 6 8 10

mAU

0

10

20

30

40

50

60

70

1

2

3

1.

Nordihydrocapsaicin

HO

H3CO NH

O

HO

H3CO NH

O

HO

H3CO NH

O


Eluent : methanol/water/acetic acid (60/40/1)Flow rate : 1.0 mL/minTemperature : 40 ℃Detection : UV at 280 nmInjection : 1 μL (1 g/3 mL)Sample : methanol extract of a commercial cayenne pepper

(F040728C)

トウガラシ中のカプサイシン類Capsaicinoids from red pepper


Eluent : methanol/water/acetic acid (60/40/0.1)Flow rate : 0.2 mL/minTemperature : 40 ℃Detection : A) UV at 280 nm

B) ESI positive-modeInjection : 1 μL (1 g/3 mL)Sample : methanol extract of a commercial cayenne pepper

(J040910A)

トウガラシ中のカプサイシン類 - LC/MSCapsaicinoids from red pepper - LC/MS

71

8 ステロイドSteroids

8

Steroids


Eluent : acetonitrile/water (40/60)Flow rate : 0.6 mL/minTemperature : 30 ℃Detection : UV at 254 nm, 0.32 AUFSInjection : 3 μL (0.25 mg/mL)

(S930322B)


1

2

3

4

Hydrocortisone

Corticosterone

Deoxycorticosterone

Progesterone


Eluent : hexane/methanol/dichloromethane (75/20/5)Flow rate : 0.6 mL/minTemperature : 30 ℃Detection : UV at 254 nm, 0.32 AUFSInjection : 3 μL (0.25 mg/mL)

(S930319D)


1

2

3

4

Progesterone

Deoxycorticosterone

Corticosterone

Hydrocortisone


Eluent : THF/water (20/80)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 260 nm, 0.13 AUFSInjection : 8 μL (0.1 mg/mL)

(S940308B)

副腎皮質ステロイドAdrenocorticosteroids

4

5

6

1

2

3

Prednisone

Cortisone

Prednisolone

Hydrocortisone

Corticosterone

Methylprednisolone



(S940222C)


1

2

3

4

5

6

7.

8

9

10

Prednisolone

Hydrocortisone

Cortisone

Corticosterone

Fluocinolone acetonide

Hydrocortisone acetate

Cortisone acetate

Deoxycorticosterone

Corticosterone acetate

Benzene



(S940310E)


5

6

7

8

1

2

3

4

Dexamethasone

Corticosterone

Beclomethasone

Triamcinolone acetonide

Hydrocortisone

Cortisone

Methylprednisolone

Betamethasone



(S940310F)


1

2

3

4

5

6

7

8

Dexamethasone

Corticosterone

Beclomethasone


Hydrocortisone

Cortisone

Methylprednisolone

Betamethasone


Eluent : chloroform/2-propanol/acetic acid (95/5/1)Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 250 nm, 0.32 AUFSInjection : 10 μL (0.25 mg/mL)

(K940530C)

ヒドロコルチゾンとプレドニゾンHydrocortisone and prednisone

1

2

Prednisone

Hydrocortisone



(O940408A)


1

2

3

4


Prednisolone acetate

Deoxycorticosterone

Fluocinonide

72


8

ステロイド

73


8

Steroids



(O940408C)


1

2

3

4



(S940420B)


1

2

3



(S940420C)


1

2

3


Eluent : acetonitrile/water/acetic acid (30/70/1)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 260 nm, 0.064 AUFSInjection : 5 μL (0.1 mg/mL)

(S940414A)


1

2


Prednisolone acetate

Deoxycorticosterone

Fluocinonide

Triamcinolone

Prednisolone


Triamcinolone

Prednisolone


Prednisolone succinate

Hydrocortisone succinate


Eluent : A)acetonitrile/THF/water (15/10/75)B)acetonitrile/water (75/25)0-50%B (0-30min, linear), 50-100%B (30-50min)


(S940303D)


1. Prednisone2. Cortisone3. Prednisolone4. Hydrocortisone5. Methylprednisolone6. Corticosterone7. Betamethasone8. Dexamethasone9. Triamcinolone acetonide

10. Beclomethasone11. Fluocinolone acetonide12. Prednisolone acetate13. Hydrocortisone acetate14. Cortisone acetate15. Deoxycorticosterone16. Corticosterone acetate17. Fluocinonide18. Betamethasone valerate19. Deoxycorticosterone acetate20. Beclomethasone dipropionate21. Ethylbenzene




(S940303B)







(S940303C)




Column : YMC-Pack SIL-06 (5μm, 6nm)250 × 4.6 mm I.D.

Eluent : hexane/THF/acetic acid (60/40/1)Flow rate : 1.5 mL/minTemperature : 30 ℃Detection : UV at 250 nm, 0.16 AUFSInjection : 10 μL (0.25 mg/mL)

(K940602B)


1

2

3

Fluoxymesterone

Methylprednisolone

Prednisolone

74


8

ステロイド

75


8

Steroids


Eluent : methanol/acetonitrile/water (45/15/40)Flow rate : 0.8 mL/minTemperature : 30 ℃Detection : UV at 210 nm, 0.064 AUFSInjection : 10 μL (0.1～2.0 mg/mL)

(S930707D)

男性ホルモンAndrogens

4

5

6

1

2

3

Fluoxymesterone

Testosterone

Dehydroisoandrosterone



(S930707C)


Methyltestosterone

trans -Androsterone

Androsterone

Fluoxymesterone

Testosterone




(S930706G)


Methyltestosterone

trans -Androsterone

Androsterone

Fluoxymesterone

Testosterone




(S940412B)

テストステロン誘導体Synthetic androgens

Testosterone

Testosterone propionate

Testosterone enanthate

4

5

6

1

2

3

Methyltestosterone

trans -Androsterone

Androsterone

4

5

6

1

2

3

1

2

3



(S940412C)

テストステロン誘導体Synthetic androgens

1

2

3

Testosterone

Testosterone propionate

Testosterone enanthate


Eluent : acetonitrile/THF/water (46/4/50)Flow rate : 0.7 mL/minTemperature : 30 ℃Detection : UV at 230 nm, 0.16 AUFSInjection : 7 μL (0.1 mg/mL)

(S930311D)

女性ホルモンEstrogens and progestins

5

6

7

8

1

2

3

4

Estrone

Progesterone

Medroxyprogesterone acetate

Mestranol

Estriol

β-Estradiol

Norethisterone

Ethinylestradiol


Eluent : acetonitrile/THF/water (46/4/50)Flow rate : 0.7 mL/minTemperature : 30 ℃Detection : UV at 230 nm, 0.16 AUFSInjection : 7 μL (0.1 mg/mL)

(S930312C)

女性ホルモンEstrogens and progestins

Estrone

Progesterone

Medroxyprogesterone acetate

Mestranol

Estriol

β-Estradiol

Norethisterone

Ethinylestradiol



(S940428E)

エストラジオール誘導体Estrogens

1

2

3

β-Estradiol

Estradiol benzoate

Estradiol dipropionate

5

6

7

8

1

2

3

4

76


8

ステロイド

77


8

Steroids



(S940428F)

エストラジオール誘導体Estrogens

1.

2.

3.



(S940427B)

ノルゲストレルとエチニルエストラジオールNorgestrel and ethinylestradiol

Ethinylestradiol

Norgestrel

Diphenyl(I.S.)



(S940426A)

ノルゲストレルとエチニルエストラジオールNorgestrel and ethinylestradiol

1.

2.

3.


Eluent : acetonitrile/water (60/40)Flow rate : 1.0 mL/minTemperature : 40 ℃Detection : UV at 240 nm, 0.04 AUFSSample : extract of pig gall were derivatized with phenacyl bromideInjection : 5 μL

(G900417B)

トンタン（豚胆）中の胆汁酸のフェナシルエステル誘導体Phenacyl esters of bile acids from pig gall

1.Cholic acid (CA)2.Hyodeoxycholic acid (HDCA)3.Chenodeoxycholic acid (CDAC)

1.

2.

3.

β-Estradiol

Estradiol benzoate

Estradiol dipropionate

Ethinylestradiol

Norgestrel

Diphenyl(I.S.)


Eluent : acetonitrile/methanol/water(5/50/45)Flow rate : 0.5 mL/minTemperature : ambientDetection : UV at 254 nm, 0.04 AUFSInjection : 5 μL (0.04 mg/mL)

(G910523H)

エクダイソンEcdysones

1.β-Ecdysone2.α-Ecdysone

Column : YMC-Pack ODS-AL (5μm, 12nm) + ODS-AL + ODS-AL +J'sphere ODS-M80 (4μm, 8nm)(250 × 4.6 mm I.D.) x 4

Eluent : methanol/water (100/2)Flow rate : 0.8 mL/minTemperature : 37 ℃Detection : UV at 210 nm, 0.16 AUFSInjection : 10 μL (0.4～1 mg/mL)

(O940701C)

ステロールSterols

4

5

6

1

2

3

Stigmasterol

Campesterol

β-Sitosterol

Brassicasterol

Cholesterol

Fucosterol



(O940706H)


1

2

3

4

5

6

Brassicasterol

Cholesterol

Fucosterol

Stigmasterol

Campesterol

β-Sitosterol



(O940706C)


Brassicasterol

Cholesterol

Fucosterol

Stigmasterol

Campesterol

β-Sitosterol

Courtesy of Dr.Furusawa, Kyoto Institute of Technology, Japan

1

2

3

4

5

6

78


8

ステロイド

79


8

Steroids



(S961125B)

強心配糖体Cardiac glycosides

1

2

3

4

5



(S961126F)




(S961129G)


1

2

3

Lanatoside C

Proscillaridin A

Lanatoside B



(S961129I)


1

2

Ouabagenin

G-Strophanthin(Ouabain)

1

2

3

4

5

80


8

ステロイド

Bufalin

HO

H3C

OH

H3C

O O

H

Cinobufagin

HO

H3C

H3C

O O

H

O

OCCH3O

Resibufogenin

HO

H3C

H3C

O O

H

O

N

H3COCH2COOH

CH3

CO Cl

Indomethacin (I.S.)　

1.

2.

3.

4.

10 200 min

1

23

4


Eluent : acetonitrile/0.1% phosphoric acid (45/55)Flow rate : 1.0 mL/minTemperature : 40 ℃Detection : UV at 300 nm, 0.08 AUFSInjection : 10 μL (0.04～0.08 mg/mL)


(S010806A)

センソ成分Steroids from toad venom

81

9 医薬品、生体関連物質Drugs, Metabolites

9

Drugs,Metab

olites


Eluent : A) 20mM KH2PO4-H3PO4 (pH 2.5)B) methanol0-20%B(0-10min), 20-90%B(10-20min), 90%B(20-25min)

Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 210 nm(0-13min), UV at 235 nm(13-25min)Injection : 10 μL (12.5～50.0 μg/mL)

(F040616A)

風邪薬成分Ingredients in a cough/cold medication

(F040524A)




Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 210 nm(0-13min), UV at 235 nm(13-25min)Injection : 15 μL (1.5 mg/mL)Sample : 70% methanol extract of a commercially available cough/cold medication

(F040616B)

市販風邪薬A commercial cough/cold medication

(F040524B)




Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 210 nm(0-12min), UV at 235 nm(12-25min)Injection : 10 μL (12.5～50.0 μg/mL)



Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 210 nm(0-12min), UV at 235 nm(12-25min)Injection : 15 μL (1.5 mg/mL)Sample : 70% methanol extract of a commercially available cough/cold medication

82


9医薬品・生体関連物質


Eluent : acetonitrile/methanol/0.1% phosphoric acid (18/15/77)Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 254 nm, 0.08 AUFSInjection : 3 μL (10 mg/mL)Sample : an extract from a commercially available cough/cold preparation

(G910523U)


1.Caffeine2.Chlorpheniramine maleate3.Narcotine4.Ethenzamide5.Bucetine


Eluent : acetonitrile/methanol/0.1% phosphoric acid (18/15/77)Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 254 nm, 0.08 AUFSInjection : 10 μL (0.02～0.22 mg/mL)

(G910523E)


1.Acetaminophen2.Caffeine3.Chloropheniramine malate4.Narcotine5.Ethenzamide6.Bucetine

(F040706B)



Eluent : acetonitrile/methanol/0.1% phosphoric acid (18/15/77)Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 254 nm, 0.08 AUFSInjection : 17 μL (0.02～0.22 mg/mL)

(G910523F)


1.Acetaminophen2.Caffeine3.Chloropheniramine malate4.Narcotine5.Ethenzamide6.Bucetine


Eluent : A) 20mM KH2PO4-K2HPO4 (pH 6.9)B) methanol5-95%B(0-20min), 95%B(20-25min)

Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 215 nm(0-13min), UV at 235 nm(13-25min)Injection : 2 μL (15 mg/mL)Sample : 70% methanol extract of a commercially available cough/cold medication

83


9

Drugs,Metab

olites


Eluent : acetonitrile/0.1% phosphoric acid (3/17)Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 254 nm, 0.16 AUFSInjection : 5 μL

(G900727D)

市販鎮痛解熱剤Pain-killing drug

1.Acetaminophen2.Caffeine3.Ethenzamide4.Bromovalerylurea


Eluent : methanol/0.1% phosphoric acid (70/30)Flow rate : 1.3 mL/minTemperature : 30 ℃Detection : UV at 254 nm, 0.64 AUFSInjection : 20 μL

(日本薬局方第12改正記載条件)

(G900727F)

インドメタシンIndometacin

1.p-Chlorobenzoic acid2.p-Hydroxybenzoic acid n-butyl ester3.Indometacin

1

2

3


Eluent : methanol/water (40/60)Flow rate : 0.7 mL/minTemperature : 30 ℃Detection : UV at 220 nm, 0.51 AUFSInjection : 5 μL (0.2～2.0 mg/mL)

(S930422D)

解熱鎮痛薬成分Analgesics

1

2

3

4

Acetaminophen

Caffeine

Ethenzamide

Apronalide


Eluent : methanol/water (40/60)Flow rate : 0.7 mL/minTemperature : 30 ℃Detection : UV at 220 nm, 0.51 AUFSInjection : 5 μL (0.2～2.0 mg/mL)

(S930422G)

解熱鎮痛薬成分Analgesics

Acetaminophen

Caffeine

Ethenzamide

Apronalide

1

2

3

4

84



1

2

3

4

1

2

3

4

0 10 20 30 min

1

2

3

4

YMC-Pack Pro C4

YMC-Pack Pro C8

YMC-Pack Pro C18

Ibuprofen

Caffeine

Ethenzamide

Bromovalerylurea

1.

2.

3.

4.

CHCOOHCH3

CHCH2CH3

CH3

N

N

N

N

O

O

CH3

H3CCH3

OC2H5

CONH2

CHCHCONHCONH2H3CH3C

Br

Column : YMC-Pack Pro C4, Pro C8 and Pro C18 (5μm,12nm) 150 × 4.6 mm I.D.


(A001019B)

解熱鎮痛薬Pain-killing drugs

Column : YMC-Pack Pro C18 (5μm,12nm) 150 × 4.6 mm I.D.

Eluent : acetonitrile/0.05% TFA (10/90)Flow rate : 1.5 mL/minDetection : UV at 210 nmInjection : 5 μL (0.1 mg/mL)

(J010528A)

コデインCodeine

Codeine

4

Tetrahydrozoline

1

2

3

100 min20

Chlorpheniramine

Benzethonium chloride

Maleic acid

1

2

3

4

CHCOOH

CHCOOH

CH2N

CH3

CH3

(OCH2CH2)2 Cl-+C

CH3

CH3

CH2C

CH3

CH3

CH3

100 min20

4

1

2

3

F040116F

F040119A

A) Standard

B) Nasal spray

N NH

CHCH2CH2NCH3CH3

N

Cl0

20

40

60

80

100

mAU

0

20

40

60

80

100

mAU


Eluent : A) 20mM KH2PO4-H3PO4 (pH 2.5)B) methanol20-90%B(0-15min), 90%B(15-20min)

Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 260 nmInjection : A) 5 μL (0.1～0.7 mg/mL)

B) 3 μL (2 times dilution of a commercially available nasal spray with water)

(F040119A)

市販点鼻薬A commercial nasal spray

4

6

5

Naphazoline

1

2

3

100 min

0

20

40

60

80

100

20

Chlorpheniramine



Lidocaine

Maleic acid

1

2

3

4

5

6

CHCOOH

CHCOOH

CH3)2N(CH2NHCCH2

OCH3

CH3

CH2

N

N

H

CHCH2CH2NCH3CH3

N

Cl

OH

COOCH3

CH2N

CH3

CH3

(OCH2CH2)2 Cl-+C

CH3

CH3

CH2C

CH3

CH3

CH3

mAU

100 min20

0

20

40

60

80

100

mAU

46

5

1

2

3

F040128A

F040119B

A) Standard

B) Nasal spray


Eluent : A) 20mM KH2PO4-H3PO4 (pH 2.5)B) methanol20-90%B(0-15min), 90%B(15-20min)

Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 260 nmInjection : A) 5 μL (0.1～0.7 mg/mL)

B) 3 μL (2 times dilution of a commercially available nasal spray with water)

(F040119B)

市販点鼻薬A commercial nasal spray

85


9

Drugs,Metab

olites


Eluent : acetonitrile/water/NH4SCN (48/52/0.5, v/v/w)Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 270 nm, 2.56 AUFSInjection : 10 μL (0.2～5 mg/mL)Sample : collunarium

(Y920610A)

鼻炎用点鼻剤Collunarium

Naphazoline hydrochloride

Chlorpheniramine maleate


1

2

3

Courtesy of NIHON ARUTU PHARMACEUTICAL CO.,LTD.


Eluent : acetonitrile/water/NH4SCN (70/30/0.5, v/v/w)Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 270 nm, 0.16 AUFSInjection : 10 μL (1.0 mg/mL)

(T920527A)

塩化ベンゼトニウムBenzethonium chloride

1.Benzethonium chloride



Eluent : acetonitrile/water/NH4SCN (70/30/0.5, v/v/w)Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 260 nm, 0.02 AUFSInjection : 10 μL (1.0 mg/mL benzalkonium chloride)

(T920529D)

塩化ベンザルコニウムBenzalkonium chloride

1.Benzalkonium chloride R=-C12H25

2.Benzalkonium chloride R=-C14H29


Benzalkonium chloride

1

2

3

1050 min

CH2N

CH3

CH3

R Cl-+

0

20

40

60

80

mAU


Eluent : A) 100mM NaCIO4/methanol (20/80)B) methanol0-50%B(0-10min)

Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 260 nmInjection : 10 μL (1.0 mg/mL)

(F031210G)

塩化ベンザルコニウムBenzalkonium chloride

1.R=-C12H25

2.R=-C14H29

3.R=-C16H33

86



1.

2.

3.

4.

5.

1

2

3

4

5

5 10 150 min

Chlorpheniramine

CHCOOH

CHCOOH

CHCH2CH2NCH3CH3

N

Cl

Maleic acid

HO

CHCH2NHCH3

Phenylephrine HCl

·HCl

HO

HO

CH ·HClCHNH2

CH3

Phenylpropanolamine HCl

N

N

N

NO

O

H3CCH3

CH3

Caffeine


Eluent : A)20mM KH2PO4 - H3PO4 (pH 2.5)B)acetonitrile0-40%B(0-10min)


鼻炎薬成分Ingredients in a drug for rhinitis

1.

2.

3.

4.

6.

5.

5 10 150 min

1

2

3

45

6

Chlorpheniramine

CHCOOH

CHCOOH

CHCH2CH2NCH3CH3

N

Cl

Pyridoxine HCl

NH3C

CH2OHCH2OH

HO

・HCl

Maleic acid

N+

CH3CH3H3C

OCN(CH3)2

OCH3SO4

-

Neostigmine methylsulfate

Naphazoline HCl

·HClCH2

N

N

HGlycyrrhizin

O

HCH3

H3C

H3C

COOHH3C

H CH3

CH3

CH3

O

H

COOH

OH

O

HO

OHH

OH H

OO

HO

H H

H

COOH

H

H

HH


Eluent : A)20mM KH2PO4 - H3PO4 (pH 2.5)B)acetonitrile0-60%B(0-10min), 60%B(10-15min)


(J010406A)(J010418B)

点眼剤成分Ingredients in an eye drop

1.

2.


Benzalkonium chloride

1

2

100 min155

0

20

40

60

80

mAU CH2N

CH3

CH3

OCH2CH2OCH2CH2 Cl-+C

CH3

CH3

CH2C

CH3

CH3

CH3

CH2N

CH3

CH3

R Cl-+(R=C14H29)


Eluent : 100mM NaCIO4/methanol (20/80)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 260 nmInjection : 10 μL (0.5～1.0 mg/mL)

殺菌剤Disinfectants

2.

1.

COOCH3

NHCNHCNH(CH2)6NHCNHCNHCl ClNH NH NH NH

・2HClChlorhexidine・2HCl

Methyl benzoate

10 20 300 min

1

2


Eluent : acetonitrile/water/TFA (30/70/0.1)Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 220 nm, 0.32 AUFSInjection : 10 μL (0.16 mg/mL, 0.1 μL/mL)

(N040206D)(F031208D)

クロルヘキシジンChlorhexidine

87


9

Drugs,Metab

olites


Eluent : 2-propanol/water/acetic acid (25/75/1)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 260 nm, 0.04 AUFSInjection : 5 μL (20 μg/mL)

(O950124A)

酸性非ステロイド性抗炎症薬Acidic non-steroidal anti-inflammatory drugs

Piroxicam

Sulindac

Tolmetin sodium dihydrate

Ketoprofen

Naproxen

1

2

3

4

5

1

2

3

1.

2.

3.

5 10 150 min


Diphenhydramine hydrochloride

CHCH2CH2NCHCOOH

CH3CH3

·�CHCOOH

N

CHOCH2CH2NCH3CH3

·�HCl

COO(CH2)3CH3

OH

Cl

Butyl -hydroxybenzoate (I.S.)p


Eluent : 20mM KH2PO4 - K2HPO4 (pH 6.9) / methanol (35/65)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 260 nm, 0.13 AUFSInjection : 5 μL (0.05～2.0 mg/mL)

抗ヒスタミン薬Histamine antagonists

1

2

3

0 5 10 min


Diphenhydramine hydrochloride

CHCOOH·�CHCOOH

CHOCH2CH2NCH3CH3

·�HCl

COO(CH2)3CH3HO

CHCH2CH2NCH3CH3

N

Cl

1.

2.

3.

Butyl -hydroxybenzoate(I.S.)p


Eluent : acetonitrile / 50mM CH3COONH4 (40/60)Flow rate : 0.2 mL/minTemperature : 37℃Detection : UV at 260 nm, 0.16 AUFSInjection : 2 μL (0.05～2.0 mg/mL)

(A980723A)(J010619A)

抗ヒスタミン薬Histamine antagonists


Eluent : methanol/water/acetic acid (65/35/1)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 260 nm, 0.04 AUFSInjection : 7 μL (160, 16, 820 μg/mL)

(O950125C)


1

2

3

Fenoprofen calcium dihydrate

Flurbiprofen

Ibuprofen

88




Eluent : methanol/water/acetic acid (65/35/1)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 260 nm, 0.032 AUFSInjection : 7 μL (6～820 μg/mL)

(A970321A)


1

2

3

4

Ketoprofen

Fenoprofen calcium dihydrate

Flurbiprofen

Ibuprofen


Eluent : methanol/water/CH3COOH (75/25/0.05)Flow rate : 0.2 mL/minTemperature : 37 ℃Detection : A) UV at 230 nm

B) ESI negative-modeInjection : 1 μL (50 μg/mL)

(J040722B)

酸性非ステロイド性解熱鎮痛抗炎症薬 - LC/MSAcidic non-steroidal anti-inflammatory drugs - LC/MS



(D960724A)

三環系抗うつ薬Tricyclic antidepressants

1

2

3

4

Doxepin hydrochloride

Desipramine hydrochloride

Imipramine hydrochloride

Amitriptyline hydrochloride


Eluent : 20mM KH2PO4 - K2HPO4 (pH 6.9)/methanol (25/75)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 254 nm, 0.16 AUFSInjection : 5 μL (0.1～0.5 mg/mL)

(A961015G)


1

2

3

4


Acenaphthene (I.S.)



89


9

Drugs,Metab

olites


Eluent : acetonitrile/water/TFA (30/70/0.1)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 254 nm, 0.064 AUFSInjection : 5 μL (0.02 mg/mL)

(O950627B)

抗精神病薬Antipsychotic drugs

1

2

3

4

5

6

7

8

Perphenazine

Prochlorperazine maleate

Promazine hydrochloride

Fluphenazine hydrochloride

Trifluoperazine hydrochloride

Ethopropazine hydrochloride(Profenamine hydrochloride)

Chlorpromazine hydrochloride

Thioridazine hydrochloride


Eluent : 50mM NH4H2PO4 - (NH4)2HPO4 (pH 6.7)/methanol (50/50)Flow rate : 0.7 mL/minTemperature : 37 ℃Detection : UV at 215 nm, 0.32 AUFSInjection : 8 μL (0.035～0.7 mg/mL)

(S960723A)

抗てんかん薬Antiepileptics

1

2

3

4

5

Ethosuximide

Primidone

Phenobarbital sodium salt

Phenytoin

Carbamazepine


Eluent : 20mM KH2PO4 - K2HPO4 (pH 6.9)/methanol (25/75)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 254 nm, 0.16 AUFSInjection : 5 μL (0.1～0.5 mg/mL)

(A961016B)


1

2

3

4


Acenaphthene (I.S.)




Acenaphthene(I.S.)



N

CH2CH2CH2NHCH3

· HCl

N

CH2CH2CH2NCH3CH3

· HCl

CHCH2CH2NCH3CH3

· HCl

1.

2.

3.

4.

1

23

4

0 5 10 min


Eluent : methanol / 20mM CH3COONH4 (75/25)Flow rate : 1.0 mL/minTemperature : 37℃Detection : UV at 254 nm, 0.32 AUFSInjection : 5 μL (0.1～0.5 mg/mL)

(A980827E)


90




Eluent : acetonitrile/water/acetic acid (60/40/1)Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 275 nm, 0.064 AUFSInjection : 8 μL (0.05～0.25 mg/mL)

(S930706B)

抗高脂血症剤Hypolipidemic drugs

1

2

3

4

Bezafibrate

Gemfibrozil

Clofibrate

Fenofibrate

Column : YMC-Pack Pro C18 (5μm,12nm)150 × 4.6 mm I.D.

Eluent : acetonitrile/0.05% formic acid (30/70)Flow rate : 1.5 mL/minDetection : UV at 240 nmInjection : 5 μL (0.1 mg/mL)

(J010528B)

ロバスタチンLovastatin



(S930706E)


1

2

3

4

Bezafibrate

Gemfibrozil

Clofibrate

Fenofibrate



(S930706C)


1

2

3

4

Bezafibrate

Gemfibrozil

Clofibrate

Fenofibrate

Lovastatin

91


9

Drugs,Metab

olites


Eluent : 20mM KH2PO4 - H3PO4 (pH 3.0)/acetonitrile/THF/TBA･Br(75/15/10/0.5, v/v/v/w)


(S930702C)

カルシウム拮抗薬Calcium antagonists

1

2

3

4

5

6

7

8

Prenylamine lactate

Bepridil HCl

Niguldipine HCl

Nifedipine

Diltiazem HCl

Verapamil HCl

Methoxyverapamil HCl

Nicardipine HCl

1.

2.

3.

Flurbiprofen

Methyl benzoate

Lidocaine

Diltiazem HCl

Verapamil HCl

4.

5.

1 2 3 4

5

CHCOOH

CH3

F

COOCH3

CH3)2N(CH2NHCCH2

OCH3

CH3

OCH3

ON

OCOCH3S

CH2CH2N(CH3)2

H3CO C

OCH3

CH(CH3)2

CN

(CH2)3NCH2CH2

CH3H3CO

OCH3

·HCl

min0 5 10

mAU

0

50

100

150

200

250

300

·HCl


Eluent : 20mM CH3COONH4-NH3(pH 8.8)/methanol (35/65) Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 220 nmInjection : 10 μL (0.04～100 μg/mL)

(F040714D)

1.

2.

3.

Flurbiprofen

Methyl benzoate

Lidocaine

Diltiazem HCl

Verapamil HCl

4.

5.

12

34

5

CHCOOH

CH3

F

COOCH3

CH3)2N(CH2NHCCH2

OCH3

CH3

OCH3

ON

OCOCH3S

CH2CH2N(CH3)2

H3CO C

OCH3

CH(CH3)2

CN

(CH2)3NCH2CH2

CH3H3CO

OCH3

·HCl

min0 5 10 15 20

mAU

0

50

100

150

200

250

300

·HCl


Eluent : 20mM CH3COONH4-NH3(pH 8.8)/methanol (35/65) Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 220 nmInjection : 10 μL (0.04～100 μg/mL)

(F040714C)

酸性および塩基性医薬品(pH 8.8)Acidic and basic drugs (pH 8.8)

1.

2.

3.

Flurbiprofen

Methyl benzoate

Lidocaine

Diltiazem HCl

Verapamil HCl

4.

5.

12

3

4

5

CHCOOH

CH3

F

COOCH3

CH3)2N(CH2NHCCH2

OCH3

CH3

OCH3

ON

OCOCH3S

CH2CH2N(CH3)2

H3CO C

OCH3

CH(CH3)2

CN

(CH2)3NCH2CH2

CH3H3CO

OCH3

·HCl

min0 5 10 15 20

mAU

0

50

100

150

200

250

300

·HCl


Eluent : 20mM CH3COONH4/methanol (35/65) Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 220 nmInjection : 10 μL (0.04～100 μg/mL)

(F040714E)



92






(S930701D)


1

2

3

4

5

6

7

8

Prenylamine lactate

Bepridil HCl

Niguldipine HCl

Nifedipine

Diltiazem HCl

Verapamil HCl


Nicardipine HCl




(S930702A)


1

2

3

4

5

6

7

8

Prenylamine lactate

Bepridil HCl

Niguldipine HCl

Nifedipine

Diltiazem HCl

Verapamil HCl


Nicardipine HCl

Nicardipine HCl

Phenytoin

NH

N

H

O

O

OCH2CHCH2NHCH(CH3)2OH

Propranolol HCl

Verapamil HCl

H3CO

N

CH3O

CH CH2

CH NOH

Quinidine

Lidocaine

CH3)2N(CH2NHCCH2

OCH3

CH3

Diltiazem HCl

OCH3

ON

OCOCH3S

CH2CH2N(CH3)2

C

OCH3

CH(CH3)2

CN

(CH2)3NCH2CH2

CH3H3CO

OCH3

NCH2COOCH2CH2N

NO2

H3C

H3COOC

CH3

CH3

�

·HCl

·HCl

·HCl

·HCl

�

1.

2.

3.

4.

5.

6.

7.

0 10 20 30 min

1

2

3

4

6 7

5

H



(S990819B)

不整脈治療薬Antiarrhythmic drugs

Nicardipine HCl

Phenytoin

NH

N

H

O

O

OCH2CHCH2NHCH(CH3)2OH

Propranolol HCl

Verapamil HCl

H3CO

N

CH3O

CH CH2

CH NOH

Quinidine

Lidocaine

CH3)2N(CH2NHCCH2

OCH3

CH3

Diltiazem HCl

OCH3

ON

OCOCH3S

CH2CH2N(CH3)2

C

OCH3

CH(CH3)2

CN

(CH2)3NCH2CH2

CH3H3CO

OCH3

NCH2COOCH2CH2N

NO2

H3C

H3COOC

CH3

CH3

�

·HCl

·HCl

·HCl

·HCl

1.

2.

3.

4.

5.

6.

7.

0 10 20 min

1

2

34

67

5

H



(S990817C)

不整脈治療薬Antiarrhythmic drugs

93


9

Drugs,Metab

olites

12

3

4

6

5

1.

2.

3.

4.

6.

5.

5 10 150 min

Lidocaine

CH3)2N(CH2NHCCH2

OCH3

CH3

H2N

O

COCH2CH3

Benzocaine(Ethyl 4-aminobenzoate)

H2N

O

COCH2CH2N(CH2CH3)2

Procaine HCl·HCl

O

COCH2CH2N(CH3)2

·HCl

NHCH2CH2CH2CH3

Tetracaine HCl

H2N

O

COCH2CH2CH2N(C4H9)2

Butacaine

N

CH2 CH

CHON

H

Cinchonine

H

H



(J010302E)

局所麻酔薬Local anesthetics


Eluent : 20mM NH4H2PO4 - H3PO4(pH 3.2)/methanol (60/40)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 280 nm, 0.16 AUFSInjection : 15 μL (0.015～0.2 mg/mL)

(A970425C)

利尿薬Diuretics

1

2

3

Hydrochlorothiazide

Chlorthalidone

Furosemide


Eluent : 20mM NH4H2PO4 - H3PO4(pH 3.2)/methanol (60/40)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 280 nm, 0.16 AUFSInjection : 15 μL (0.015～0.2 mg/mL)

(A970426D)

利尿薬Diuretics

1

2

3

Hydrochlorothiazide

Chlorthalidone

Furosemide

1.

2.

3.

1

2

3

S

N

NHO O

Cl

SO

OH2N

Hydrochlorothiazide

NH

O

ClSO2NH2

HO

Chlorthalidone

NHCH2OCO2H

ClSH2NO

OFurosemide

H

0 5 10 min


Eluent : methanol / 1% acetic acid (40/60)Flow rate : 0.2 mL/minTemperature : 37 ℃Detection : UV at 280 nm, 0.08 AUFSInjection : 3 μL (0.015～0.2 mg/mL)

(A980716A)

利尿薬Diuretics

94




Eluent : methanol/water/acetic acid (20/80/1)Flow rate : 1.0 mL/minTemperature : ambient(25 ℃)Detection : UV at 300 nm, 0.04 AUFSInjection : 5 μL (16 μg/mL)

(O950411B)

葉酸及び葉酸拮抗物質Folic acid and its antagonists

Aminopterin

Methotrexate

Folic acid

1

2

3


Eluent : 10mM NH4H2PO4


(T920924E)

制がん剤および関連化合物Antineoplastic and related compounds

1

2

3

4

Uracil

5-Fluorouracil

Deoxyuridine

5-Fluoro-2'-deoxyuridine




(T920924G)

制がん剤および関連化合物Antineoplastic and related compounds

Uracil

5-Fluorouracil

Deoxyuridine

5-Fluoro-2'-deoxyuridine

1

2

3

4


Eluent : methanol/10mM KH2PO4 (10/60)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 254 nm, 0.16 AUFSInjection : 7 μL (0.125 mg/mL)

(K930311C)

抗ＨＩＶヌクレオシド誘導体Anti-human immunodeficiency virus (HIV) agents

DDC

DDI

D4T

AZT

1

2

3

4

95


9

Drugs,Metab

olites



(K930311A)


1

2

3

4

DDC

DDI

D4T

AZT



(K930311F)


1

2

3

4

DDC

DDI

D4T

AZT


Eluent : acetonitrile/20mM KH2PO4 (5/95)Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 260 nm, 0.64 AUFSInjection : 13 μL (0.2 mg/mL)

(K940125I)

ニコチン酸構造類縁体Nicotinic acid analogues

1

2

3

4

5

Isonicotinic acid

Nicotinic acid

Isonicotinic acid hydrazide

Nicotinamide

N' -Methylnicotinamide



(K940125K)

ニコチン酸構造類縁体Nicotinic acid analogues

Isonicotinic acid

Nicotinic acid

Isonicotinic acid hydrazide

Nicotinamide

N' -Methylnicotinamide

1

2

3

4

5

96




Eluent : 100mM KH2PO4 - Na2HPO4 (pH 7.0)/methanol (75/25)Flow rate : 0.8 mL/minTemperature : 30 ℃Detection : UV at 270 nm, 0.13 AUFSInjection : 3 μL (0.1～0.8 mg/mL)

日本薬局方第12改正記載条件

(S930617D)

殺菌薬Disinfectants

1

2

3

4

Benzoic acid

Salicylic acid

Theophylline (I.S.)

Phenol


Eluent : 20mM K2HPO4 - KH2PO4 (pH 6.9)/acetonitrile (92/8)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 254 nm, 0.32 AUFSInjection : 10 μL (30～300 μg/mL)

(T931217C)

塩基性化合物Basic compounds

Uracil

Procainamide

N-Acetylprocainamide

4'-Hydroxyacetophenone

1

2

3

4


Eluent : acetonitrile / 0.05% formic acid (3 / 97)Flow rate : 1.5 mL/minDetection : UV at 254 nmInjection : 5μL (0.1 mg/mL)

(J010528C)

アシクロビルAcyclovir

5 10 15 200 min

12

L-Histidine hydrochloride

Carnitine chloride

Cl-(CH3)3N+CH2CHCH2COOH

OH

N

N

H

CH2CHCOOHNH2

·HCl

1.

2.


Eluent : 50mM NaH2PO4-H3PO4 (pH2.6) containing 15mM 1-pentanesulfonic acid sodium salt

Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 210 nm, 0.064 AUFSInjection : 4 μL (0.2～20 mg/mL)

(F000629A)

塩化カルニチンCarnitine chloride

Acyclovir

97


9

Drugs,Metab

olites




(S930728J)

キサンチンオキシダーゼ阻害剤および関連化合物Xanthine oxidase inhibitor and related compounds

1

2

3

4

5

Uric acid

Hypoxanthine

Xanthine

Oxipurinol

Allopurinol




(S930729B)

キサンチンオキシダーゼ阻害剤および関連化合物Xanthine oxidase inhibitor and related compounds

1

2

3

4

5.

Uric acid

Hypoxanthine

Xanthine

Oxipurinol

Allopurinol


Eluent : acetonitrile/0.75% formic acid (4/1)Flow rate : 1.0 mL/minTemperature : ambientDetection : A) UV at 255 nm, B) UV at 295nmInjection : 10 μL

(F041213A)

カフェイン代謝物Caffeine metabolites


Eluent : acetonitrile/0.75% formic acid (4/1)Flow rate : 1.0 mL/minTemperature : ambientDetection : A) UV at 255 nm, B) UV at 295nmInjection : Standard 10 μL

Urine sample 10 μL

(F041213B)

尿中のカフェイン代謝物Caffeine metabolites in human urine

98




Eluent : 10mM CH3COOHFlow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 200 nm, 0.16 AUFSInjection : 4 μL (0.10～0.71 mg/mL)

(G910618A)

プリン代謝物Purine catabolites

1.Allantoin2.Allantoic acid3.Uric acid


Eluent : 10mM CH3COOHFlow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 200 nm, 0.16 AUFSInjection : 4 μL (0.10～0.71 mg/mL)

(G910607G)

プリン代謝物Purine catabolites

1.Allantoin2.Allantoic acid3.Uric acid

10 20 30 400 min

1

2

3

4

5

67

8

9


Eluent : A)50mM KH2PO4-H3PO4(pH 3.1)B)50mM KH2PO4-H3PO4(pH 3.1)/acetonitrile (90/10)0%B (0-5min), 0-100%B (5-25min,linear), 100%B (25-35min)


(F000817A)

カテコールアミンCatecholamines

1. Norepinephrine hydrochloride2. Epinephrine3. 3,4-Dihydroxyphenylalanine

(DOPA)4. Dopamine hydrochloride5. 4-Hydroxy-3-methoxymandelic acid6. 3-Methoxytyramine hydrochloride7. 4-Hydroxy-3-methoxyphenylglycol8. 3,4-Dihydroxyphenylacetic acid9. Homovanillic acid


Eluent : 20mM NH4H2PO4 - H3PO4 (pH 3.2)/methanol (80/20)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 225 nm, 0.16 AUFSInjection : 10 μL (0.04～0.07 mg/mL)

(A961114A)

馬尿酸類Hippuric and methylhippuric acids

1

2

3

4

5

Mandelic acid

Hippuric acid

o-Methylhippuric acid

m-Methylhippuric acid

p-Methylhippuric acid

99


9

Drugs,Metab

olites


Eluent : 20mM NH4H2PO4 - H3PO4 (pH 3.2)/methanol (80/20)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 225 nm, 0.16 AUFSInjection : 10 μL (0.04～0.07 mg/mL)

(A961119A)

馬尿酸類Hippuric and methylhippuric acids

1

2

3

4

5

Mandelic acid

Hippuric acid

o-Methylhippuric acid

m-Methylhippuric acid

p-Methylhippuric acid

100

10 抗菌剤、抗生物質Antibiotics

抗菌剤・抗生物質

10


Eluent : A)acetonitrile/50mM NH4H2PO4 (10/90)B)acetonitrile/50mM NH4H2PO4 (80/20)25%B (0-5min), 25-100%B (5-15min), 100%B (15-20min)


(K940614B)

合成抗菌剤Antibacterial agents

1. Olaquindox2. Carbadox3. Thiamphenicol4. Sulfamerazine5. Sulfadimidine6. Sulfamonomethoxine7. Furazolidone8. Oxolinic acid9. Sulfaquinoxaline

10. Sulfadimethoxine11. Nalidixic acid12. Piromidic acid


Eluent : A)acetonitrile/50mM NH4H2PO4 (10/90)B)acetonitrile/50mM NH4H2PO4 (80/20)25%B (0-5min), 25-100%B (5-15min), 100%B (15-20min)


(K940615C)


1. Olaquindox2. Carbadox3. Thiamphenicol4. Sulfamerazine5. Sulfadimidine6. Sulfamonomethoxine7. Furazolidone8. Oxolinic acid9. Sulfaquinoxaline

10. Sulfadimethoxine11. Nalidixic acid12. Piromidic acid


Eluent : acetonitrile/50mM NH4H2PO4 (15/85)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 230 nm, 0.16 AUFSInjection : 5 μL (20 μg/mL)

(O950320G)


1

2

3

Diaveridine

Trimethoprim

Pyrimethamine


Eluent : THF/50mM NH4H2PO4 (10/90)Flow rate : 0.5 mL/minTemperature : 37 ℃Detection : UV at 275 nm, 0.16 AUFSInjection : 3 μL (50 μg/mL)

(O950306E)

サルファ剤Sulfonamides

1

2

3

4

5

6

7

8

9

10

Sulfapyridine

Sulfamerazine

Sulfamethizole

Sulfamethoxypyridazine

Sulfamethazine

Sulfaguanidine

Sulfanilamide

Sulfisomidine

Sulfathiazole

Sulfadiazine

101


Antibiotics

10


Eluent : 50mM NH4H2PO4 - (NH4)2HPO4 (pH 6.2)/THF (90/10)Flow rate : 0.5 mL/minTemperature : 37 ℃Detection : UV at 275 nm, 0.16 AUFSInjection : 10 μL (18 μg/mL)

(O950324L)


Sulfathiazole

Trimethoprim

Sulfapyridine

Sulfamerazine

Sulfamethoxypyridazine

Sulfamethazine

Sulfaguanidine

Sulfamethizole

Sulfanilamide

Sulfisomidine

Sulfadiazine


Eluent : THF/water/acetic acid (30/70/1)Flow rate : 0.5 mL/minTemperature : 37 ℃Detection : UV at 275 nm, 0.16 AUFSInjection : 5 μL (42 μg/mL)

(O950302C)


1

2

3

4

5

6

7

8

Sulfamethazine

Sulfadoxine

Sulfamonomethoxine hydrate

Sulfisoxazole

Sulfamethoxazole

Sulfachloropyridazine

Sulfadimethoxine

Sulfaquinoxaline sodium


Eluent : THF/acetonitrile/50mM NH4H2PO4 (15/5/80)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 275 nm, 0.16 AUFSInjection : 3 μL (50 μg/mL)

(O950217D)


Sulfamonomethoxine hydrate

Sulfadoxine

Sulfamethoxazole

Sulfadimethoxine

Sulfaquinoxaline sodium

Sulfisomidine

Sulfathiazole

Sulfadiazine

Sulfamerazine

Sulfamethazine

1

2

3

4

5

6

7

8

9

10

1

2

3

4

6

75

Sulfathiazole

2.

3.

Sulfamerazine

4.

Sulfadimidine

5.1.

Sulfisomidine Sulfamonomethoxine

6.

Sulfamethoxazole

7.

Sulfadimethoxine

5 10 15 200 min

NH2 SO2NHN

N

CH3

CH3

N

S

N

N

CH3

CH3

CH3

NN

OCH3

ONCH3

OCH3

OCH3

NH2 SO2NH

NH2 SO2NH

NH2 SO2NH

NH2 SO2NH

NH2 SO2NH

NH2 SO2NH

N

N

NN


Eluent : 10mM CH3COOH - CH3COONH4 (pH 4.6) / acetonitrile(85/15)


(J010321F)


1

2

3

4

5

6

7

8

9

10

11

102



10


Eluent : 50mM NH4H2PO4 - (NH4)2HPO4 (pH 6.7)/tetrahydrofuran (90/10)containing 20mM TBA･Br


(O950428D)

ナリジクス酸系抗生物質Nalidixic acid antibiotics 1

2

3

4

5

6

7

8

Pipemidic acid

Enoxacin

Cinoxacin

Norfloxacin

Ofloxacin

Nalidixic acid

Oxolinic acid

Piromidic acid


Eluent : A)acetonitrile/50mM NH4H2PO4 (10/90)B)acetonitrile/50mM NH4H2PO4 (50/50)0-55%B (0-30min,linear), 55%B (30-40min)


(G910523M)

抗生物質Antibiotics

1.Ampicilline2.Cephalexin3.Oxytetracycline hydrochloride4.Tetracycline5.Chlorotetracycline6.Chloramphenichol7.Oxolinic acid8.Doxorubicin hydrochloride9.Nalidixic acid


Eluent : 50mM NH4H2PO4 - (NH4)2HPO4 (pH 6.7)/tetrahydrofuran (90/10)containing 20mM TBA･Br


(O950428A)

ナリジクス酸系抗生物質Nalidixic acid antibiotics 1

2

3

4

5

6

7

8

Pipemidic acid

Enoxacin

Cinoxacin

Norfloxacin

Ofloxacin

Nalidixic acid

Oxolinic acid

Piromidic acid

1

2

3

4

6

5

N

NNH2 SO2NH

Sulfathiazole

2.

3.

Sulfamerazine

4.

Sulfadimidine

5.1.

Sulfadiazine Sulfamonomethoxine

6.

Sulfamethoxazole

N

S

N

N

CH3

CH3

CH3

NN

OCH3

ONCH3

NH2 SO2NH

NH2 SO2NH

NH2 SO2NH

NH2 SO2NH

NH2 SO2NH

N

N

5 100 min


Eluent : 10mM CH3COOH - CH3COONH4 (pH 4.6) / acetonitrile(85/15)


(J010130D)


103


Antibiotics

10


Eluent : acetonitrile/100mM KH2PO4 (10/90)Flow rate : 0.8 mL/minTemperature : 37 ℃Detection : UV at 260 nm, 0.16 AUFSInjection : 5 μL (0.12～0.35 mg/mL)

(O940715F)

セフェム系抗生物質Cephalosporin antibiotics

1. Cefsulodin sodium2. Cephalexin hydrate3. Cefaclor4. Cephradine5. Cephaloglycin6. Cephapirin sodium7. Cefotaxime sodium8. Cephaloridine9. Cefazolin sodium

10. Cefuroxime sodium11. Cefoxitin sodium



(O940719A)




2.

3.

4.

1

2

3

4

1.

OCOOH

N N

Nalidixic acid

N

OCOOHO

O

Oxolinic acid

NN

N

OCOOH

N

Piromidic acid

Cinoxacin

N

OCOOHO

O N

5 10 150 min

C2H5

C2H5

H3C

C2H5

C2H5


Eluent : 10mM CH3COOH - CH3COONH4(pH 4.3) / acetonitrile(75/25)


(A010324A)

ナリジクス酸系抗生物質Nalidixic acid antibiotics

2.

3.

4.

1

2

3

4

1.

5 10 150 min

OCOOH

N N

Nalidixic acid

N

OCOOHO

O

Oxolinic acid

NN

N

OCOOH

N

Piromidic acid

Cinoxacin

N

OCOOHO

O N

C2H5

C2H5

C2H5

C2H5

H3C


Eluent : acetonitrile / 50mM NaH2PO4 (25/75)Flow rate : 0.2 mL/minTemperature : 37 ℃Detection : UV at 260 nm, 0.128 AUFSInjection : 1 μL (0.02～0.1 mg/mL)

(A010324C)

ナリジクス酸系抗生物質Nalidixic acid antibiotics

104



10



(O940927I)


1

2

3

4

5

Cefadroxil

Cephalosporin C potassium

Cefatrizine propylene glycol

Cephalexin hydrate

Cephaloridine



(O940927B)


1

2

3

4

5

Cefadroxil



Cephalexin hydrate

Cephaloridine



(O940927C)


Cefadroxil



Cephalexin hydrate

Cephaloridine

1

2

3

4

5



(O940718B)




105


Antibiotics

10



(O940927G)


Cefadroxil



Cephalexin hydrate

Cephaloridine

1

2

3

4

5



(O941025E)


1

2

3

4

5

Cefadroxil



Cephalexin hydrate

Cephaloridine


Eluent : THF/50mM NH4H2PO4 (15/85)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 260 nm, 0.16 AUFSInjection : 6 μL (0.17 mg/mL)

(O940928P)


1

2

3

Cefoperazone sodium

Cephalothin sodium

Cefamandole sodium



(O940928Q)


1

2

3

Cefoperazone sodium

Cephalothin sodium

Cefamandole sodium

106



10



(O940928R)


Cefoperazone sodium

Cephalothin sodium

Cefamandole sodium

1

2

3



(O940929D)


1

2

3

Cefoperazone sodium

Cephalothin sodium

Cefamandole sodium



(O940928S)


Cefoperazone sodium

Cephalothin sodium

Cefamandole sodium

1

2

3


Eluent : methanol/50mM NH4H2PO4 (30/70)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 260 nm, 0.16 AUFSInjection : 5 μL (0.08, 0.16 mg/mL)

(O940928G)


1

2

Ceftriaxone sodium

Cefotaxime sodium

107


Antibiotics

10



(O940928F)


Ceftriaxone sodium

Cefotaxime sodium



(O940929K)


1

2

Ceftriaxone sodium

Cefotaxime sodium



(O940720A)


1

2

3

Cephaloglycin

Cefotaxime sodium

Cephapirin sodium

1

23

4

5 10 15 200 min

CH

CH3

S

N

NHCO

NH2COOH

CH

CH2OCOCH3

S

N

NHCO

NH2COOH

Cephalexin

Cephaloglycin

CH

Cl

S

N

NHCO

NH2COOH

Cefaclor

COS CH2

CH2

S

NO

NH

COOHCephaloridine

O

O

O

1

2

3

4

+N


Eluent : acetonitrile/20mM KH2PO4 (10/90) Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 260 nm, 0.32 AUFSInjection : 15 μL (0.1～0.2 mg/mL)

(S000717B)


1

2

108



10


Eluent : acetonitrile/50mM KH2PO4 (20/80)Flow rate : 1.8 mL/minTemperature : 37 ℃Detection : UV at 260 nm, 0.32 AUFSInjection : 1.2 μL (8, 12 mg/mL)

(O940728M)

セフォキシチンナトリウムCefoxitin sodium

1

2

Caffeine (I.S.)

Cefoxitin sodium


Eluent : acetonitrile/50mM KH2PO4 (20/80)Flow rate : 0.34 mL/minTemperature : 37 ℃Detection : UV at 260 nm, 0.32 AUFSInjection : 1.2 μL (1.6, 2.4 mg/mL)

(O940728P)


Caffeine (I.S.)

Cefoxitin sodium

1

2

1

2

1.

2.

CH

CH2

S

N

(CH2)3OCNH

COCH3COOK

O O

OH


ResorcinolOH

HOOC

5 10 150 min

H2N


Eluent : acetonitrile/20mM KH2PO4 (5/95) Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 260 nm, 0.16 AUFSInjection : 10μL (0.2 mg/mL)

(S000714B)

セファロスポリンCCephalosporin C

1

2

5 10 150 min

C

CH2S

S

N

NHCO

NOCH3COONa

O N

NN

O

OHS

N

OH

Ceftriaxone sodium

Phenol (I.S.)

1.

2.

H2N H3C


Eluent : methanol / 10mM H3PO4 (25/75)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 260 nm, 0.32 AUFSInjection : 10 μL (0.1, 0.4 mg/mL)

(S000726B)

セフトリアキソンCeftriaxone

109


Antibiotics

10


Eluent : 50mM NaH2PO4 - H3PO4 (pH 2.3)/acetonitrile (67/33)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 220 nm, 0.26 AUFSInjection : 8 μL (0.13 mg/mL)

(O950908A)

ペニシリン系抗生物質Penicillin antibiotics

1

2

3

4

5

6

7

8

Bacampicillin hydrocloride

Methicillin sodium

Penicillin G potassium(Benzylpenicillin potassium)

Penicillin V potassium(Phenoxymethylpenicillin potassium)

Phenethicillin potassium(Phenoxyethylpenicillin potassium)

Cloxacillin sodium

Propicillin potassium

Dicloxacillin sodium


Eluent : 50mM NaH2PO4 - H3PO4 (pH 2.3)/acetonitrile (80/20)containing 20mM sodium 1-heptanesulfonate

Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 220 nm, 0.13 AUFSInjection : 2 μL (0.3, 0.7 mg/mL)

(O950920C)

ペニシリン系抗生物質Penicillin antibiotics

1

2

Amoxicillin hydrate

Ampicillin anhydrous

1.

2.

5 10 150 min

CH

COOH

CH3CH3

S

NO

NHCO

NH2

HO

Amoxicillin

N

NN

NO

O

Caffeine

CH3

H3CCH3

1

2


Eluent : acetonitrile / water / TFA (10/90/0.1)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 220 nm, 0.32 AUFSInjection : 10 μL (0.2, 0.1 mg/mL)

(S000622D)

アモキシシリンAmoxicillin


Eluent : acetonitrile/50mM KH2PO4 (20/80)Flow rate : 0.34 mL/minTemperature : 37 ℃Detection : UV at 260 nm, 0.32 AUFSInjection : 1.2 μL (1.6, 2.4 mg/mL)

(O940729A)


1

2

Caffeine (I.S.)

Cefoxitin sodium

110



10


Eluent : acetonitrile/methanol/100mM (NH4)2HPO4(45/15/40)Flow rate : 1.0 mL/minTemperature : 45 ℃Detection : UV at 210 nm, 0.26 AUFSInjection : 15 μL (2.5 mg/mL)

(S930910B)

エリスロマイシンErythromycin antibiotic drugs

1.Erythromycin2.Erythromycin ethylsuccinate3.Erythromycin estolate

1.

2.

CH

CH3

S

N

NHCO

NH2COOH

O

Cephalexin (MW:347.1)

Amoxicillin (MW:365.1)

CH

COOH

CH3CH3

S

NO

NHCO

NH2

HO

1

2100

%

0

(m/z)TIC

366

348

2.0 min

0

Column : LC/MS Cartridge Column VAPro C18 (3μm, 12nm)20 × 2.0 mm I.D.

Eluent : A) water/formic acid (100/0.05)B) methanol/formic acid (100/0.05)10-90%B(0-0.5min), 90%B(0.5-1.0min)

Flow rate : 0.6 mL/minTemperature : 37 ℃Detection : ESI-positive modeInjection : 2 μL

(A020620A)

抗生物質の高速分離 - LC/MSRapid separation of antibiotics - LC/MS


Eluent : acetonitrile/10mM oxalic acid (10/90)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 280 nm, 0.13 AUFSInjection : 6 μL (0.2 mg/mL)

(S970524D)

テトラサイクリン系抗生物質Tetracycline antibiotics

1

2

Oxytetracycline hydrochloride

Tetracycline hydrochloride

10 200 min

1

2

OH

NH2

H

O

O

HO

H

H

H

HHHO

OH H

CH3

CH3

O

CH2OH

Cl

O

NH

O

CH3

H3C

NH

CH3

OHN

CNH2

ONH

O

O

NH

HN

OCl

HO

O NH

CO2H

OH

OHOH

Vancomycin hydrochloride

4'-Hydroxyacetophenone

OH

O

CO CH3

OH

1.

2.

・HCl


Eluent : 20mM KH2PO4 - K2HPO4 (pH 6.9) / acetonitrile(88/12)


(S000728B)

バンコマイシンVancomycin

111

11 農薬、環境関連物質Pesticides, Environmental

Pesticid

es,Enviro

nmental

11

Column : YMC-Pack MB-ODS150 × 2.1 mm I.D.

Eluent : acetonitrile/water (60/40)Flow rate : 0.3 mL/minTemperature : 30 ℃Detection : UV at 220 nm, 0.01 AUFSInjection : 10μL (1.0 mg/L)

(G910223E)

ベンスリドO,O -Di-isopropyl S-2-benzenesulphonamidoethylphosphorodithioate


Eluent : acetonitrile/water (40/60)Flow rate : 0.3 mL/minTemperature : 30 ℃Detection : UV at 260 nm, 0.01 AUFSInjection : 10 μL (2.0 mg/L)

(G910223D)

チウラムBis(dimethylthiocarbamoyl)disulfide

1.Thiram


Eluent : THF/water/TFA (10/90/0.05)Flow rate : 0.3 mL/minTemperature : 30 ℃Detection : UV at 260 nm, 0.01 AUFSInjection : 10 μL (1.0 mg/L)

(G910223C)

アシュラムMethyl sulfanilylcarbamate

1.Asulam

5 100 min



(A010717A)

オキシン銅Copper 8-quinolinolate

1. Copper 8-quinolinolate

1.Bensulide

112


農薬・環境関連物質

11


Eluent : A)acetonitrile/50mM NH4H2PO4 (20/80)B)acetonitrile/50mM NH4H2PO4 (65/35)0-100%B (0-60min, linear)


(O941018B)

除草剤Herbicides

1. Atrazine-desisopropyl2. Atrazine-desethyl3. Crimidine4. Hexazinone5. Metoxuron6. Simazine7. Cyanazine8. Atraton9. Atrazine

10. Isoproturon11. Diuron12. Prometon13. Metobromuron14. Terbumeton15. Metazachlor16. Ametryn17. Sebuthylazine18. Propazine19. Terbuthylazine20. Linuron21. Prometryn22. Terbutryn23. Metolachlor

3

4

6

5

1

2

10 200 min

7

8

9

O

N

N

HN

NHCOCH3

SNHCOCH3H2NO

O

NHCNHCOCH3

NHCNHCOCH3

(CH3)2NCSSCN(CH3)2

NHCNH

N

NO

O

CHNHCH(CH3)2

S S

S

S

OCl OCHCOH

CH3

OCH3

Cl

Cl

CuO

N

O

O

O

O

O

SNHCH2CH2SPO

O

SOCH(CH3)2OCH(CH3)2

H3C

Oxine-copper(Copper 8-quinolinolate)

Carbendazim (MBC)

Asulam

Mecoprop (MCPP)

Bensulide (SAP)

Iprodion

Siduron

Thiophanate-methyl

Thiram

1

2

3

4

6

7

8

9

5


Eluent : A) 50mM KH2PO4-H3PO4 (pH 3.0)B) acetonitrile15-65%B (0-10min), 65%B (10-20min)

Flow rate : 1.0 mL/minTemperature : 40 ℃Detection : UV at 270 nm (0-6.5 min), 230 nm (6.5-20 min)

0.08 AUFSInjection : 10 μL (10 μg/mL)

(N040107A)

水質規制農薬9成分Analysis of 9 pesticides


Eluent : acetonitrile/water/TFA (25/75/0.1)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 230 nm, 0.16 AUFSInjection : 5 μL (0.04 mg/mL)

(O941020H)

アトラジン誘導体Atrazine derivatives

1

2

3

4

Atrazine-2-hydroxy

Atrazine-desisopropyl

Atrazine-desethyl

Atrazine



(O941102F)

トリアジン系除草剤Triazine herbicides

1. Cyanazine2. Simazine3. Atraton4. Atrazine5. Prometon6. Terbumeton7. Sebuthylazine8. Propazine9. Terbuthylazine

10. Prometryn11. Terbutryn

113


Pesticid

es,Enviro

nmental

11



(A970210B)

トリアジン系除草剤Triazine herbicides

1

2

3

4

5

6

7

8

9

Sebuthylazine

Propazine

Terbuthylazine

Prometryn

Terbutryn

Simazine

Atrazine

Prometon

Terbumeton


Eluent : THF/water (30/70)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 260 nm, 0.16 AUFSInjection : 4 μL (0.2 mg/mL)

(O941028B)

尿素系除草剤Urea herbicides

1

2

3

4

5

Metoxuron

Isoproturon

Diuron

Metobromuron

Linuron


Eluent : 2-propanol/THF/water (25/15/60)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 210 nm, 0.16 AUFSInjection : 8 μL (0.05 mg/mL)

(O941108C)

酸アミド系除草剤Acid amide herbicides

1

2

3

4

Metazachlor

Metolachlor

Alachlor

Butachlor



(O941124G)

カルバメート系除草剤Carbamate herbicides

1

2

3

Propham

Swep

Chloropropham

114



11


Eluent : methanol/20mM NH4H2PO4 (70/30)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 230 nm, 0.32 AUFSInjection : 10 μL (0.02～0.20 mg/mL)

(A970217A)

防かび剤Fungicides

1

2

3

4

Thiabendazole

o-Phenylphenol

Imazalil

Diphenyl


Eluent : methanol/20mM NH4H2PO4 (70/30)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 230 nm, 0.32 AUFSInjection : 10 μL (0.02～0.20 mg/mL)

(A970219A)

防かび剤Fungicides

Thiabendazole

o-Phenylphenol

Imazalil

Diphenyl



(B931007A)

イマザリルImazalil

1.Imazalil

1

2

3

4

1

2

3

4

5

1.

2.

3.

4.

5.

5 100 min

(CH3)2NC C

O

SCH3

O

NOCNHCH3

Oxamyl

CH3S

O

NOCNHCH3

Aldicarb

C(CH3)2CH

O

OCNHCH3O

O

CH3

CH3

Bendiocarb

O

OCNHCH3CH2SC2H5

Ethiofencarb

CH3H3C

N(CH3)2

Pirimicarb

NN(CH3)2NCO

O


Eluent : methanol / 20mM KH2PO4 (45/55)Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 240 nm, 0.64 AUFSInjection : 5 μL (0.1～1.5 mg/mL)

(J010220D)

カルバメート系殺虫剤Carbamate insecticides

115


Pesticid

es,Enviro

nmental

11

min

mV

200

100

10 200

0

1

2

3

4

5

CnH2n+1

SO3Na

1. Sodium decylbenzenesulfonate (n=10)2. Sodium undecylbenzenesulfonate (n=11)3. Sodium dodecylbenzenesulfonate (n=12)4. Sodium tridecylbenzenesulfonate (n=13)5. Sodium tetradecylbenzenesulfonate (n=14)


Eluent : acetonitrile/water (65/35) containing 0.1M NaClO4

Flow rate : 1.0 mL/minTemperature : 40 ℃Detection : FLS at Ex 221 nm, Em 284 nmInjection : 20 μL (2.0 μg/mL)

平成15年改正水質基準の検査方法準拠

(N040324D)

陰イオン界面活性剤Anionic surfactants

A) Standard

B) Laundry detergent

0

0

100

200

300

0 10

0

100

200

CnH2n+1

SO3Na

20

300

20

1

2

34

5

10 min

mV

min

mV

1

23

4


N040326B

N040325A



Flow rate : 1.0 mL/minTemperature : 40 ℃Detection : FLS at Ex 221 nm, Em 284 nmInjection : 20 μLSample : A) 2.0 μg/mL

B) 25 μg/mL (water solution of laundry detergent powder)

(N040325A)

市販洗濯洗剤中の陰イオン界面活性剤Anionic surfactants in a commercial laundry detergent

A) Standard

B) Cleanser

0

20 min

300

mV

200

100

0

20 min

300

mV

200

100

0

0 10

10

N040326B

N040323B

CnH2n+1

SO3Na


1

2

3

4

1

2

34

5



Flow rate : 1.0 mL/minTemperature : 40 ℃Detection : FLS at Ex 221 nm, Em 284 nmInjection : 20 μLSample : A) 2.0 μg/mL

B) 250 μg/mL (water solution of powder cleanser)

(N040323B)

市販クレンザー中の陰イオン界面活性剤Anionic surfactants in a commercial cleanser

0 10 20 30 min

0

10

20

30

mAU

C8H17 (OCH2CH2)nOH

Triton X-100


Eluent : THF/acetonitrile/water (10/45/45)Flow rate : 1.0 mL/minTemperature : 25 ℃Detection : UV at 254nmInjection : 10 μL (20 mg/mL)

(N040308D)

非イオン性界面活性剤Nonionic surfactants

116



11


Eluent : A)tetrahydrofuran/water (10/90)B)acetonitrile35%B (0-7min), 35-65%B (7-18min, linear), 100%B (18-19min),35%B (19-35min)


(T920302A)

アルデヒドおよびケトン類の2,4-DNPH誘導体2,4-Dinitrophenylhydrazones of aldehydes and ketones

1.Formaldehyde 2,4-DNPH2.Acetaldehyde 2,4-DNPH3.Acetone 2,4-DNPH4.Acrolein 2,4-DNPH5.Propionaldehyde 2,4-DNPH6.Crotonaldehyde 2,4-DNPH7.Methylethylketone 2,4-DNPH8.Isobutyraldehyde 2,4-DNPH9.Benzaldehyde 2,4-DNPH

10.n-Valeraldehyde 2,4-DNPH11.p-Tolualdehyde 2,4-DNPH12.Capronaldehyde 2,4-DNPH


Eluent : acetonitrile/water (10/90)Flow rate : 1.0 mL/minTemperature : ambient (23 ℃)Detection : UV at 260 nm, 0.02 AUFSInjection : 15 μL (5.0 mg/mL)Sample : Triton X-100

(G920109C)

非イオン性界面活性剤Nonionic surfactant

1.Triton X-100


Eluent : acetonitrile/water(10/90)Flow rate : 1.0 mL/minTemperature : ambient (22 ℃)Detection : RI, 64 x 10-6 RIU/FSInjection : 100 μL (1.67 mg/mL)

(G920123A)

ポリエチレングリコールPolyethyleneglycols

1.PEG #20,000 (MW 20,000)2.PEG #6,000 (MW 8,400)3.PEG #4,000 (MW 3,350)4.PEG #1,000 (MW 1,000)5.PEG #300 (MW 300)6.Ethyleneglycol (MW 62)


Eluent : A)tetrahydrofuran/water (20/80)B)acetonitrile/methanol (90/10)30-67%B (0-15min, Linear), 30%B (15-25min)


(T931130D)

アルデヒドおよびケトン類の2,4-DNPH誘導体2,4-dinitrophenylhydrazones of aldehydes and ketones

1.Formaldehyde 2,4-DNPH2.Acetaldehyde 2,4-DNPH3.Acetone 2,4-DNPH4.Acrolein 2,4-DNPH5.Propionaldehyde 2,4-DNPH6.Crotonaldehyde 2,4-DNPH7.Methylethylketone 2,4-DNPH8.Isobutyraldehyde 2,4-DNPH9.Benzaldehyde 2,4-DNPH

10.n-Valeraldehyde 2,4-DNPH11.p-Tolualdehyde 2,4-DNPH12.Capronaldehyde 2,4-DNPH

117


Pesticid

es,Enviro

nmental

11



(K930918A)

多環芳香族炭化水素Polyaromatic hydrocarbons

1.Benzene2.Naphthalene3.7,12-Dimethylbenzo[a]anthracene4.Acenaphthylene5.Diphenyl6.Fluorene7.Naphthacene8.Acenaphthene9.Phenanthrene

10.Anthrathene11.Fluoranthene12.Pyrene13.Triphenylene14.Chrysene15.Perylene16.Benzo[e]pyrene17.Benzo[b]fluoranthene18.Benzo[k]fluoranthene19.Benzo[a]pyrene20.Dibenzo[a,h]anthracene21.Benzo[g,h,i]perylene22.Coronene



(K930918B)


1.Benzene2.Naphthalene3.Acenaphthylene4.7,12-Dimethylbenzo[a]anthracene5.Diphenyl6.Fluorene7.Acenaphthene8.Naphthacene9.Phenanthrene



Eluent : A)acetonitrile/water (50/50)B)acetonitrile30%B (0-5min), 30-100%B (5-40min), 100%B (40-50min)

Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 254 nm, 0.08 AUFSInjection : 7 μL

(K930918C)


1.Benzene2.Naphthalene3.7,12-Dimethylbenzo[a]anthracene4.Acenaphthylene5.Diphenyl6.Fluorene7.Naphthacene8.Acenaphthene9.Phenanthrene


118

12 その他The others

その他

12


Eluent : acetonitrile/water/acetic acid (5/90/5)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 285 nm, 0.16 AUFSInjection : 5 μL (0.1 mg/mL)

(O940530D)

ヒドロキシ安息香酸Hydroxybenzoic acids

1

2

3

DHBA, Dihydroxybenzoic acidHBA, Hydroxybenzoic acid

7

8

9

4

5

6

2,3-DHBA

Benzoic acid

Salicylic acid

2,5-DHBA

2,4-DHBA

m-HBA

3,5-DHBA

3,4-DHBA

p-HBA



(O940607C)

レゾルシン酸異性体Resorcylic acid isomers

1

2

3

4

α-Resorcylic acid

β-Resorcylic acid

Resorcinol

γ-Resorcylic acid



(O940606J)

レゾルシン酸異性体Resorcylic acid isomers

1

2

3

4

α-Resorcylic acid

β-Resorcylic acid

Resorcinol

γ-Resorcylic acid


Eluent : tetrahydrofuran/water/acetic acid (40/60/1)Flow rate : 0.5 mL/minTemperature : ambient (26 ℃)Detection : UV at 260 nm, 0.32 AUFSInjection : 10 μL (0.04～0.2 mg/mL)

(O950511B)

安息香酸誘導体Benzoic acid derivatives

1

2

3

4

5

p-Ethoxybenzoic acid

p-Propoxybenzoic acid

p-Isopropylbenzoic acid(Cuminic acid)

p-Buthoxybenzoic acid

p-tert-Butylbenzoic acid

119


Theothers

12


Eluent : tetrahydrofuran/water/acetic acid (40/60/1)Flow rate : 0.5 mL/minTemperature : ambient (26 ℃)Detection : UV at 260 nm, 0.32 AUFSInjection : 10 μL (0.04～0.2 mg/mL)

(O950511C)

安息香酸誘導体Benzoic acid derivatives

1

2

3

4

5

p-Ethoxybenzoic acid

p-Propoxybenzoic acid

p-Isopropylbenzoic acid(Cuminic acid)

p-Buthoxybenzoic acid

p-tert-Butylbenzoic acid


Eluent : acetonitrile/water/acetic acid (20/80/1)Flow rate : 1.0 mL/minTemperature : ambient (25 ℃)Detection : UV at 285 nm, 0.16 AUFSInjection : 10 μL (0.17 mg/mL)

(O950508A)

メトキシ安息香酸異性体Methoxybenzoic acid isomers

1

2

3

o-Methoxybenzoic acid(o-Anisic acid)

p-Methoxybenzoic acid(p-Anisic acid)

m-Methoxybenzoic acid(m-Anisic acid)



(O950508B)

メトキシ安息香酸異性体Methoxybenzoic acid isomers

1

2

3

o-Methoxybenzoic acid(o-Anisic acid)

p-Methoxybenzoic acid(p-Anisic acid)

m-Methoxybenzoic acid(m-Anisic acid)



(O950509B)

クロロ安息香酸異性体Chlorobenzoic acid isomers

o-Chlorobenzoic acid

m-Chlorobenzoic acid

p-Chlorobenzoic acid

1

2

3

120


その他

12



(O950509D)

クロロ安息香酸異性体Chlorobenzoic acid isomers

1

2

3

o-Chlorobenzoic acid

m-Chlorobenzoic acid

p-Chlorobenzoic acid


Eluent : 5mM acetic acidFlow rate : 1.0 mL/minTemperature : 25 ℃Detection : UV at 280 nm, 0.32 AUFSInjection : 20 μL

(H890627A)

ポリフェノールPolyphenols

1.Pyrogallol2.Hydroquinone3.Catechol4.Phenol

2

4

3

1

0 10 20min



(A971112B)

フタル酸異性体Phthalic acid isomers

1

2

3

Phthalic acid

Terephthalic acid

Isophthalic acid



(A971112A)

フタル酸異性体Phthalic acid isomers

Phthalic acid

Terephthalic acid

Isophthalic acid

1

2

3

121


Theothers

12


Eluent : 20% ethyl acetate in hexaneFlow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 254 nm, 0.08 AUFSInjection : 20 μL (0.33 mg/mL)

(T921019B)

ジメチルフェノール異性体Dimethylphenol isomers

1

3

5

2

4

6

2,5-Dimethylphenol

2,3-Dimethylphenol

3,4-Dimethylphenol

2,6-Dimethylphenol

2,4-Dimethylphenol

3,5-Dimethylphenol


Eluent : 10% ethyl acetate in hexaneFlow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 254 nm, 0.08 AUFSInjection : 20 μL (0.33 mg/mL)

(T921020C)

ジメチルフェノール異性体Dimethylphenol isomers

2,5-Dimethylphenol

2,3-Dimethylphenol

3,4-Dimethylphenol

2,6-Dimethylphenol

2,4-Dimethylphenol

3,5-Dimethylphenol

1

3

5

2

4

6


Eluent : 2-propanol/water/TFA (40/60/0.1)Flow rate : 0.4 mL/minTemperature : 37 ℃Detection : UV at 230 nm, 0.13 AUFSInjection : 5 μL (0.04 mg/mL)

(O950616I)

フェノール誘導体Phenol derivatives 1

2

3

4

5

6

2,6-Dinitrophenol

2,4,6-Trinitrophenol

2,4-Dinitrophenol

2,6-Dichlorophenol

2,4-Dichlorophenol

2,4,6-Trichlorophenol


Eluent : tetrahydrofuran/water/acetic acid (35/65/1)Flow rate : 0.5 mL/minTemperature : 37 ℃Detection : UV at 240 nm, 0.13 AUFSInjection : 4 μL (0.08 mg/mL)

(O950614C)

フェノール誘導体Phenol derivatives

o-Nitrophenol

o-Chlorophenol

m-Nitrophenol

p-Nitrophenol

m-Chlorophenol

p-Chlorophenol

1

2

3

4

5

6

122


その他

12



(O951025C)

クレゾール異性体Cresol isomers

1

2

3

m-Cresol

p-Cresol

o-Cresol

1

2

3

1.

2.

3.

10 200 min

m-Chlorophenol

OH

Cl

o-Chlorophenol

OHCl

OH

Clp-Chlorophenol



(J011113D)

クロロフェノール異性体Chlorophenol isomers


Eluent : tetrahydrofuran/water/acetic acid (35/65/1)Flow rate : 0.5 mL/minTemperature : 37 ℃Detection : UV at 240 nm, 0.13 AUFSInjection : 4 μL (0.08 mg/mL)

(O950614F)

フェノール誘導体Phenol derivatives

o-Nitrophenol

o-Chlorophenol

m-Nitrophenol

p-Nitrophenol

m-Chlorophenol

p-Chlorophenol



(O951025B)

クレゾール異性体Cresol isomers

1

2

3

m-Cresol

p-Cresol

o-Cresol

1

2

3

4

5

6

123


Theothers

12

1

2

3

1.

2.

3.

m-Chloroacetophenone

COCH3

Cl

o-Chloroacetophenone

COCH3Cl

COCH3

Clp-Chloroacetophenone

10 200 min


Eluent : acetonitrile/water (40/60)Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : UV at 254 nm, 0.32 AUFSInjection : 8.0 μL (0.05～0.1 μL/mL)

(J011026B)

クロロアセトフェノン異性体Chloroacetophenone isomers

1

2

3

1.

2.

3.

10 200 min

m-Terphenyl

o-Terphenyl

p-Terphenyl



(J020719E)

ターフェニル異性体Terphenyl isomers


Eluent : hexane/2-propanol (70/30)Flow rate : 1.0 mL/minTemperature : ambient (25 ℃)Detection : UV at 350 nm, 0.08 AUFSInjection : 4 μL (0.125 mg/mL)

(S930119B)

フラーレン同族体Fullerenes

1

2

Buckminsterfullerene(C60)

(5,6)-Fullerene(C70)


Eluent : hexane/2-propanol (50/50)Flow rate : 1.0 mL/minTemperature : ambient (25 ℃)Detection : UV at 350 nm, 0.08 AUFSInjection : 4 μL (0.125 mg/mL)

(S930128B)

フラーレン同族体Fullerenes

Buckminsterfullerene(C60)

(5,6)-Fullerene(C70)

1

2

124


その他

12


Eluent : 100mM Na2HPO4/100mM NaOH (60/40, pH 12.0)Flow rate : 0.6 mL/minTemperature : 20 ℃Detection : UV at 215 nm, 0.32 AUFSInjection : 50 μL (0.2～3.0 mg/mL)

(M920124B)

アミノアルコールAminoalcohols

1.Ethanolamine2.Diethanolamine3.N-Methylethanolamine4.Triethanolamine5.N,N-Dimethylethanolamine


Eluent : acetonitrile/water (70/30) containig 0.01% PANand 10mM tetrabutylammonium bromide

Flow rate : 1.0 mL/minTemperature : 30 ℃Detection : VIS at 565 nm, 0.08 AUFSInjection : 12 μL (25～92 μg/mL)

(T910520A)

1- (2-ピリジルアゾ) -2-ナフトール(PAN)金属キレートMetal-PAN chelates

1.Co (Ⅱ)-1- (2-Pyridylazo)-2-naphthol2.Ni (Ⅱ)-1- (2-Pyridylazo)-2-naphthol3.Fe (Ⅲ)-1- (2-Pyridylazo)-2-naphthol4.Zn (Ⅱ)-1- (2-Pyridylazo)-2-naphthol


Eluent : 20mM KH2PO4 - K2HPO4 (pH7.6)/methanol (40/60)Flow rate : 1.0 mL/minTemperature : 40 ℃Detection : UV at 254 nm, 0.32 AUFS

(M910604I)

配位性化合物Coordination compounds

1. Uracil2. Acetylacetone3. 8-Quinolinol


Eluent : 20mM NH4H2PO4 containing 1mM EDTA･2Na/acetonitrile (65/35)Flow rate : 1.0 mL/minTemperature : 37 ℃Detection : UV at 320 nm, 0.16 AUFSInjection : 10 μL (0.08, 0.25 mg/mL)

(O960507F)

ヒノキチオールHinokitiol

Hinokitiol

1-Naphthol(I.S.)

1

2

125


Theothers

12


Eluent : 0.4% HClO4 (pH 1.3)Flow rate : 0.5 mL/minTemperature : 25 ℃Detection : UV at 210 nm, 0.32 AUFSInjection : 15 μL (0.4～7.5 mg/mL)

(M920108A)

尿素及び関連化合物Urea and related compounds

1.Urea2.Propionamide3.Ethylurea4.1,3-Diethylurea5.1,1-Diethylurea



(S961007C)

芳香族アミンAromatic amines

1

2

3

4

5

6

p-Phenylenediamine

p-Aminophenol

m-Phenylenediamine

m-Aminophenol

o-Phenylenediamine

o-Aminophenol



(S961004E)

芳香族アミンAromatic amines

p-Phenylenediamine

p-Aminophenol

m-Phenylenediamine

m-Aminophenol

o-Phenylenediamine

o-Aminophenol

1

2

3

4

5

6

126

13 光学異性体Optical isomers

光学異性体

13

Column : YMC CHIRAL NEA (R)250 × 4.6 mm I.D.

Eluent : acetonitrile/water (50/50)Flow rate : 1.0 mL/minTemperature : ambientDetection : UV at 235 nmInjection : 1 μL (2.8 mg/mL)

ビナフトール1,1'-Bi-2-naphthol



トリフルオロアンスリルエタノール2,2,2-Trifluoro-1-(9-anthryl)-ethanol



ヘキソバルビタールHexobarbital


Eluent : 0.5M NaClO4 - HClO4 (pH 2.0)/acetonitrile (70/30)Flow rate : 1.0 mL/minTemperature : ambientDetection : UV at 254 nmInjection : 10 μL (1.5 mg/mL)

CBZ-フェニルアラニンCBZ-Phenylalanine(Z-Phe-OH)

(N020605A) (N020605B)

(N020605C) (N020605D)

127


Opticaliso

mers

13


Eluent : acetonitrile/0.5M NaClO4 (40/60)Flow rate : 1.0 mL/minTemperature : ambientDetection : UV at 254 nmInjection : 5 μL (1.5 mg/mL)

塩酸プロプラノロールPropranolol hydrochloride


Eluent : acetonitrile/0.5M NaClO4 (40/60)Flow rate : 1.0 mL/minTemperature : ambientDetection : UV at 268 nmInjection : 10 μL (1.4 mg/mL)

塩酸ケタミンKetamine hydrochloride

Column : YMC CHIRAL β-CD BR250 × 4.6 mm I.D.

Eluent : water/acetic acid (100/1)Flow rate : 1.0 mL/minTemperature : 25 ℃Detection : UV at 254 nmInjection : 2 μL (1 mg/mL)

クロルタリドンChlorthalidone


Eluent : 0.53M AcOH - 0.53M TEA (pH 4.0)/acetonitrile (10/90)(TEA=triethylamine)

Flow rate : 1.0 mL/minTemperature : 25 ℃Detection : UV at 254 nmInjection : 5 μL (1 mg/mL)

フェノプロフェンカルシウムFenoprofen calcium salt; hydrate

(N020605E) (N020605F)

(N020605H)(N020605G)

128


光学異性体

13




フルルビプロフェンFlurbiprofen


Eluent : 0.2M AcOH - 0.2M TEA (pH 5.6)/methanol (70/30)(TEA=triethylamine)


ヘキソバルビタールHexobarbital


Eluent : 0.1M AcOHaq - TEAaq (pH 4.0)/acetonitrile (10/90)(TEA=triethylamine)


イブプロフェンIbuprofen




インダパミドIndapamide

(N020605I) (N020605J)

(N020605L)(N020605K)

129


Opticaliso

mers

13




ケトプロフェンKetoprofen




CBZ-フェニルアラニンCBZ-Phenylalanine (Z-Phe-OH)

Column : YMC CHIRAL γ-CD BR250 × 4.6 mm I.D.



フルルビプロフェンFlurbiprofen




ナフチルエチルアルコール1-(1-Naphthyl)-ethylalcohol

(N020605M) (N020605N)

(N020605O) (N020605P)

130


光学異性体

13




フェニルエチルアルコールPhenylethylalcohol


Eluent : acetonitrile/methanol/acetic acid/triethylamine (99/1/0.3/0.25)Flow rate : 1.0 mL/minTemperature : 25 ℃Detection : UV at 254 nmInjection : 5 μL (1 mg/mL)

塩酸プロプラノロールPropranolol hydrochloride




トレーガー塩基Troger's base




CBZ-フェニルアラニンCBZ-Phenylalanine (Z-Phe-OH)

(N020605Q) (N020605R)

(N020605S) (N020605T)

化合物名インデックス�化合物名インデックス�

Index of AnalytesIndex of Analytes

APPLICATION DATA COLLECTIONS

131

AAcacetin 64Acenaphthene 88, 89, 117Acenaphthylene 117Acetaldehyde 2,4-DNPH 116 Acetaminophen 81, 82, 83Acetic acid 52Acetoacetyl-CoA 18Acetone 2,4-DNPH 116Acetylacetone 1245-Acetylamino-6-amino-3-methyluracil 97N-Acetyl-chitobiose 28N-Acetyl-chitopentaose 28N-Acetyl-chitotetraose 28N-Acetyl-chitotriose 28Acetyl-CoA 18N-Acetylglucosamine 28N-Acetyl procainamide 96Acid red 57Acrolein 2,4-DNPH 116Acyclovir 96Adenine 34Adenosine 34, 35Adenylate kinase 50, 515'-ADP 35ADP-D-glucose 37Aflatoxin B1 69Aflatoxin B2 69Aflatoxin G1 69Aflatoxin G2 69Alachlor 113Alanine 39L-Alanine 39, 40Albiflorin 62Aldicarb 114Allantoic acid 98Allantoin 17, 98Allopurinol 97Amaranth 57Ametryn 112p -Aminobenzoic acid (PABA) 14, 15, 17o -Aminophenol 125m -Aminophenol 125p -Aminophenol 125Aminopterin 94Amitriptyline hydrochloride 88, 89Amoxicillin 109, 1105'-AMP 35, 36Ampicillin 102, 109Amyloid β-protein 45Androsterone 75trans -Androsterone 75Angiotensin I 41, 42, 43[Asn1,Val5] Angiotensin I 41[Val5] Angiotensin I 41Angiotensin II 41, 43, 44, 49Angiotensin III 41, 44, 45o -Anisic acid 119m -Anisic acid 119p -Anisic acid 119Annatto extract 58

Anthrathene 117Apigenin 64Apronalide 83Arabitol 21Arachidonic acid 53Arbutin 61, 68Arginine 39L-Arginine 39, 40L-Ascorbic acid 13, 15, 16, 17, 19,

81L-Ascorbic acid 2,4-DNPH 19Asparagine 39L-Asparagine 40Aspartame 58, 59Aspartic acid 39L-Aspartic acid 39, 40Asulam 111, 1125'-ATP 35Atraton 112Atrazine 112, 113Atrazine-desethyl 112Atrazine-desisopropyl 112Atrazine-2-hydroxy 112Atropine sulfate 60AZT 94, 95

BBacampicillin hydrochloride 109Baicalein 64Baicalin 61, 65BAM-12P 43Barbaloin 61Beclomethasone 72, 74Beclomethasone dipropionate 74Bendiocarb 114Benfotiamine 16, 17, 82Bensulide 111, 112Benzaldehyde 2,4-DNPH 116Benzalkonium chloride 85, 86Benzene 71, 117Benzethonium chloride 84, 85, 86Benzo[a]pyrene 117Benzo[b]fluoranthene 117Benzo[e]pyrene 117Benzo[g,h,i]perylene 117Benzo[k]fluoranthene 117Benzocaine 93Benzoic acid 54, 58, 59, 96, 118Benzylpenicillin potassium 109Bepridil hydrochloride 91, 92Betamethasone 72, 74Betamethasone valerate 74Bezafibrate 90BHA (Butyl hydroxyanisol) 56, 57BHT (Dibutyl hydroxytoluene) 56, 571,1'-Bi-2-naphthol 126Biotin 14, 15Bisbentiamine 17Bombesin 42Bradykinin 42, 43, 44, 45Brassicasterol 78

化合物名インデックス Index of Analytes

132

Brilliant Blue FCF 57Bromovalerylurea 83, 84BSA 45, 46Bucetine 82Bufalin 80Butacaine 93Butachlor 113p -Buthoxybenzoic acid 118, 119p -tert-Butylbenzoic acid 118, 119tert -Butylhydroquinone (TBHQ) 55, 56Butyl hydroxyanisol (BHA) 56, 57Butyl p-hydroxybenzoate 55, 68, 69, 83, 87

CCaffeine 13, 14, 58, 59, 61,

81, 82, 83, 84, 86, 97, 108, 109

Campesterol 78Capronaldehyde 2,4-DNPH 116Capsaicin 70Carbadox 100Carbamazepine 89Carbendazim 112Carnitine chloride 96α-Carotene 8β-Carotene 8, 9Casein 50(+)-Catechin 66Catechol 120CBZ-Phenylalanine (Z-Phe-OH) 126, 129, 130CCK-Octapeptide 445'-CDP 35CDP-D-glucose 37Cefaclor 103, 104, 107Cefadroxil 104, 105Cefamandole sodium 105, 106Cefatrizine propylene glycol 104, 105Cefazolin sodium 103, 104Cefoperazone sodium 105, 106Cefotaxime sodium 103, 104, 106, 107Cefoxitin sodium 103, 104, 108, 109Cefsulodin sodium 103, 104Ceftriaxone sodium 106, 107, 108Cefuroxime sodium 103, 104Cephalexin 102, 103, 104, 105,

107, 110Cephaloglycin 103, 104, 107Cephaloridine 103, 104, 105, 107Cephalosporin C potassium 104, 105, 108Cephalothin sodium 105, 106Cephapirin sodium 103, 104, 107Cephradine 103, 104Chenodeoxycholic acid (CDAC) 77Chloramphenichol 102Chlorhexidine hydrochloride 86o -Chloroacetophenone 123m -Chloroacetophenone 123p -Chloroacetophenone 123o -Chlorobenzoic acid 119, 120m -Chlorobenzoic acid 119, 120p -Chlorobenzoic acid 83, 119, 120

o -Chlorophenol 121, 122m -Chlorophenol 121, 122p -Chlorophenol 121, 122Chlorophyll a 69Chlorophyll b 69Chloropropham 113Chlorotetracycline 102Chlorpheniramine maleate 81, 82, 84, 85, 86,

87Chlorpromazine hydrochloride 89Chlorthalidone 93, 127Cholecalciferol 8, 9, 10 Cholesterol 78Cholic acid (CA) 77Chondroitin, Na salt 31Chondroitin sulfate A, Na salt 31Chondroitin sulfate B, Na salt 31Chondroitin sulfate C, Na salt 31Chrysene 117Chrysin 64Chrysoeriol 64α-Chymotrypsinogen A 46Cinchonidine 60Cinchonine 60, 93Cinobufagin 80Cinoxacin 102, 103Citric acid 52Clofibrate 90Cloxacillin sodium 1095'-CMP 35, 36Codeine 84Coenzyme A 18Coenzyme A glutathione disulfide 18Coenzyme A oxidized 18Color additives (Acid red) 57Color additives (Amaranth) 57Color additives (Brilliant Blue FCF) 57Color additives (Erythrosine) 57Color additives (Fast Green FCF) 57Color additives (Indigo Carmine) 57Color additives (New Coccine) 57Color additives (Phloxine) 57Color additives (Rose Bengale) 57Color additives (Sunset Yellow FCF) 57Color additives (Tartrazine) 57Conalbumin 46Copper 8-quinolinolate 111, 112Coronene 117Corticosterone 71, 72, 74Corticosterone acetate 71, 74Cortisone 71, 72, 74Cortisone acetate 71, 74o -Cresol 122m -Cresol 122p -Cresol 122Crimidine 112Crotonaldehyde 2,4-DNPH 1165'-CTP 35Cuminic acid 118, 119Cyanazine 112, 113Cyanocobalamin 13, 14, 15, 16α-Cyclodextrin 26, 27β-Cyclodextrin 26, 27

INDEX

133

γ-Cyclodextrin 26, 27L-Cystine 39, 40Cytidine 34, 35Cytochrome c 45, 46, 48, 50, 51Cytochrome c (dimer) 48Cytochrome c (hexamer) 48Cytochrome c (tetramer) 48Cytochrome c (trimer) 48Cytocine 34

DD4T 94, 95Daidzein 66Daidzin 665'-dAMP 355'-dCMP 35DDC 94, 95DDI 94, 95Dehydroacetic acid 54 Dehydroisoandrosterone 75Deoxyadenosine 34, 35Deoxycorticosterone 71, 72, 73, 74Deoxycorticosterone acetate 74Deoxycytidine 34, 35Deoxyguanosine 34, 35Deoxyinosine 34, 35Deoxyuridine 94Desipramine hydrochloride 88, 89Dexamethasone 72, 74Dextromethorphan hydrobromide 815'-dGMP 35ΔDi-0S 21ΔDi-4S 21ΔDi-6S 21ΔDi-diSD 21ΔDi-diSE 21ΔDi-triS 21Diaveridine 100Dibenzo[a,h]anthracene 117Dibutyl hydroxytoluene (BHT) 56, 57Dicethiamine hydrochloride 172,4-Dichlorophenol 1212,6-Dichlorophenol 121Dicloxacillin sodium 109Diethanolamine 1241,1-Diethylurea 1251,3-Diethylurea 125Digitoxigenin 79Digitoxin 61, 79Digoxigenin 79Digoxigenin-bis-digitoxoside 79Digoxin 79Dihydrocapsaicin 70Dihydrocodeine phosphate 81, 82Dihydroquinidine 60Dihydroquinine 602,3-Dihydroxybenzoic acid 1182,4-Dihydroxybenzoic acid 1182,5-Dihydroxybenzoic acid 1183,4-Dihydroxybenzoic acid 1183,5-Dihydroxybenzoic acid 118

3,4-Dihydroxyphenylacetic acid 983,4-Dihydroxyphenylalanine (DOPA) 98Diltiazem hydrochloride 91, 927,12-Dimethylbenzo[a]anthracene 117O -Dimethyl-β-cyclodextrin 27, 28N,N -Dimethylethanolamine 1242,3-Dimethylphenol 1212,4-Dimethylphenol 1212,5-Dimethylphenol 1212,6-Dimethylphenol 1213,4-Dimethylphenol 1213,5-Dimethylphenol 1211,7-Dimethyluric acid 971,9-Dimethyluric acid 972,4-Dinitrophenol 1212,6-Dinitrophenol 121Diphenhydramine hydrochloride 87Diphenyl 77, 114, 117Diuron 112, 113Docosahexaenoic acid 53n -Dodecyl gallate (DG) 55, 56DOPA (3,4-Dihydroxyphenylalanine) 98Dopamine hydrochloride 98Doxepin hydrochloride 88Doxorubicin hydrochloride 102Dynorphin A (Human,1-13) 42

Eα-Ecdysone 78β-Ecdysone 78Cu(II)-EDTA 55Fe(III)-EDTA 55Eicosapentaenoic acid 53Eicosatrienoic acid 53Eledoisin related peptide 42α-Endorphin 43β-Endorphin (Human) 43γ-Endorphin 43[D-Ala2,Met5]-Enkephalin 43Leu-Enkephalin 42, 43, 44, 45Met-Enkephalin 42, 43, 44, 45[D-Ala2,Met5]-Enkephalinamide 43Enolase 50, 51Enoxacin 102(-)-Epicatechin 66(-)-Epicatechin gallate 66(-)-Epigallocatechin 66(-)-Epigallocatechin gallate 66Epinephrine 98Ergocalciferol 8, 9, 10Erythorbic acid 16, 19Erythritol 21, 22Erythromycin 110Erythromycin estolate 110Erythromycin ethylsuccinate 110Erythrosine 57Esculine 61β-Estradiol 76, 77Estradiol benzoate 76, 77Estradiol dipropionate 76, 77Estriol 76


134

Estrone 76Ethanolamine 124Ethenzamide 81, 82 , 83, 84Ethinylestradiol 76, 77Ethiofencarb 114Ethopropazine hydrochloride 89Ethosuximide 89p -Ethoxybenzoic acid 118, 119Ethyl 4-aminobenzoate 93Ethylbenzene 74Ethyleneglycol 116Ethyl p-hydroxybenzoate 55Ethylurea 125

FFast Green FCF 57Fenofibrate 90Fenoprofen 87, 88, 127Flavin adenine dinucleotide disodium 18Flavin mononucleotide sodium salt(Flavin sodium phosphate) 13, 14, 18Fluocinolone acetonide 71, 72, 73, 74Fluocinonide 72, 73, 74Fluoranthene 117Fluorene 1175-Fluorocytosine 345-Fluoro-2'-deoxyuridine 945-Fluoroorotic acid 355-Fluorouracil 34, 94Fluoxymesterone 74, 75Fluphenazine hydrochloride 89Flurbiprofen 87, 88, 91, 128,

129Folic acid 13, 94Formaldehyde 2,4-DNPH 1161-Fructofuranosyl-D-nystose 23Fructose 20, 22Fucose 20Fucosterol 78Fullerene (C60) 123Fullerene (C70) 123Fumaric acid 52, 82Furazolidone 100Furosemide 93

GGalactose 20Gallic acid 685'-GDP 35GDP-D-mannose 37Gemfibrozil 90Genistein 66Genistin 66Ginsenoside-Rb1 63, 64Ginsenoside-Rg1 63, 64Glucose 20, 22, 24, 26Glutamate dehydrogenase 50, 51Glutamic acid 39L-Glutamic acid 39, 40

Glutamine 39Glutathione (GSH) 40Glutathione disulfide (GSSG) 40Glycerol 21Glycine 39, 40, 49Glycolic acid 52Glycyrrhizic acid (Glycyrrhizin) 59, 61, 865'-GMP 35, 365'-GTP 35Guanine 34Guanosine 34, 35

HHeparan sulfate, Na salt 32Heparin, Na salt 32Hesperidin 65Hexazinone 112Hexobarbital 126, 128Hinokitiol 124Hippuric acid 98, 99Histidine 39L-Histidine 39, 40, 96Homovanillic acid 98Hordenine sulfate 60Human IgA 46, 47Human IgG 46, 47, 48Human IgM(κ), myeloma protein 46, 47, 48Hyaluronic acid, Na salt 32Hydrochlorothiazide 93Hydrocortisone 71, 72, 74Hydrocortisone acetate 71, 74Hydrocortisone succinate 73Hydroquinone 68, 1204'-Hydroxyacetophenone(p-) 62, 96, 110m -Hydroxybenzoic acid 118p -Hydroxybenzoic acid 1184-Hydroxy-3-methoxymandelic acid 984-Hydroxy-3-methoxyphenylglycol 984-Hydroxymethyl-2,6-di-tert-butylphenol(HMBP) 55, 563-Hydroxy-3-methylglutaryl-CoA (HMG-CoA) 18Hyodeoxycholic acid (HDCA) 77Hypoxanthine 34, 97

IIbuprofen 81, 84, 87, 88, 128Imazalil 114Imipramine hydrochloride 88, 895'-IMP 35, 36Indapamide 128Indigo Carmine 57Indometacin (Indomethacin) 80, 83Inosine 34, 35Inositol 21Insulin 42, 45Insulin (Bovine) 41, 42, 45, 49Insulin (Equine) 41, 42Insulin (Human) 41, 42

INDEX

135

Insulin (Porcine) 41, 42Insulin (Sheep) 41, 42Insulin chain B 44Iprodion 112Isobutyl p-hydroxybenzoate 55Isobutyraldehyde 2,4-DNPH 116Isoleucine 39L-Isoleucine 39, 40Isomalt 21Isonicotinic acid 95Isonicotinic acid hydrazide 95Isophthalic acid 120p -Isopropylbenzoic acid 118, 119Isopropyl p-hydroxybenzoate 55Isoproturon 112, 113Isoquercitrin 65

KKaempferol 64Keratan polysulfate, Na salt 33Keratan sulfate, Na salt 321-Kestose 22, 23Ketamine hydrochloride 127Ketoprofen 87, 88, 129

Lα-Lactalbumin 45Lactate dehydrogenase 50, 51Lactic acid 52Lactitol 21, 22β-Lactoglobulin A 46β-Lactoglobulin B 46Lactose 20Lactosyl fructoside 23, 24Lanatoside B 79Lanatoside C 79Leucine 39L-Leucine 39, 40Lidocaine 84, 91, 92, 93Linoleic acid 53γ-Linolenic acid 53Linuron 112, 113Lipoxidase 46Lovastatin 90Luteolin 64Lysine 39L-Lysine 39, 40Lysozyme 45, 46

MMaleic acid 52, 81, 84, 86L-Malic acid 52Malonic acid 52Malonyl-CoA 18Maltitol 21, 22Maltododecaose (G12) 25, 26Maltoheptaose (G7) 24, 26

Maltohexaose (G6) 24Maltopentadecaose (G15) 25, 26Maltopentaose (G5) 24, 26Maltose (G2) 20, 22, 24Maltotetradecaose (G14) 25, 26Maltotetraose (G4) 24Maltotridecaose (G13) 25, 26Maltotriose (G3) 24, 26Maltoundecaose (G11) 25, 26Mandelic acid 98, 99Mannitol 21Mannose 20α-Mating factor 42, 45Mecoprop 112Medroxyprogesterone acetate 76Menadione 8Menaquinone 8, 12Mestranol 76Metazachlor 112, 113Methicillin sodium 109Methionine 39L-Methionine 39, 40Methotrexate 94o -Methoxybenzoic acid 119m -Methoxybenzoic acid 119p -Methoxybenzoic acid 1193-Methoxytyramine hydrochloride 98Methoxyverapamil hydrochloride 91, 92Methyl benzoate 86, 91dl-Methylephedrine hydrochloride 81, 82N -Methylethanolamine 124Methylethylketone 2,4-DNPH 116o -Methylhippuric acid 98, 99m -Methylhippuric acid 98, 99p -Methylhippuric acid 98, 99Methyl p-hydroxybenzoate 55, 65, 84Methyl linoleate 53Methyl linolenate 53Methyl γ-linolenate 53Methylmalonyl-CoA 18N' -Methylnicotinamide 95Methyl oleate 53Methylprednisolone 71, 72, 74Methyltestosterone 751-Methyluric acid 971-Methylxanthine 97Metobromuron 112, 113Metolachlor 112, 113Metoxuron 112, 113Myoglobin 45, 46Peptide fragments from myoglobin 48Myricetin 64

NNAD 18NADH 18NADP 18NADPH 18Nalidixic acid 100, 102, 103Naphazoline hydrochloride 84, 85, 86Naphthacene 117


136

Naphthalene 1171-Naphthol 1241-(1-Naphthyl)-ethylalcohol 129Naproxen 87Narcotine 60, 82Naringin 65Narirutin 65Neohesperidin 65Neostigmine methylsulfate 86Neurotensin 42, 43, 49New Coccine 57Nicardipine hydrochloride 91, 92Nicotinamide 13, 14, 15, 16, 17,

18, 95β-Nicotinamide adenine dinucleotide 18Nicotinamide adenine dinucleotidephosphate oxidized form 18Nicotinamide adenine dinucleotide phosphate reduced form 18β-Nicotinamide adenine dinucleotidereduced form 18Nicotinic acid 13, 16, 17, 18, 95 Nifedipine 91, 92Niguldipine hydrochloride 91, 92o -Nitrophenol 121, 122m -Nitrophenol 121, 122p -Nitrophenol 121, 122Nordihydrocapsaicin 70Nordihydroguaiaretic acid (NDGA) 55, 56Norepinephrine hydrochloride 98Norethisterone 76Norfloxacin 102Norgestrel 77Noscapine 60, 82Nystose 22, 23

On -Octyl gallate (OG) 55, 56Ofloxacin 102Olaquindox 100Oligonucleotides (c-Ha-ras/12) 36Orotic acid 15, 35Ouabagenin 79Ouabain 61, 79Ovalbumin 45, 46Oxamyl 114Oxine-copper 111, 112Oxipurinol 97Oxolinic acid 100, 102, 103Oxytetracycline hydrochloride 102, 110Oxytocin 42, 43

PPaeoniflorin 62Paeonol 68, 69Palatinit 21Palatinose 21, 22Pantothenic acid, Na salt 13Pantothenyl alcohol (Panthenol) 13, 17, 18

Papaverine chloride 60Paraxanthine 97PA-Sugar Chain 001 28, 29PA-Sugar Chain 002 28, 29PA-Sugar Chain 014 28, 29PEG #300 (MW 300) 116PEG #1,000 (MW 1,000) 116PEG #4,000 (MW 3,350) 116PEG #6,000 (MW 8,400) 116PEG #20,000 (MW 20,000) 116Penicillin G potassium 109Penicillin V potassium 109Pentaglycine 49Perphenazine 89Perylene 117Phenanthrene 117Phenethicillin potassium 109Phenobarbital sodium salt 89Phenol 61, 96, 108, 120Phenoxyethylpenicillin potassium 109Phenoxymethylpenicillin potassium 109Phenylalanine 39L-Phenylalanine 39, 40o -Phenylenediamine 125m -Phenylenediamine 125p -Phenylenediamine 125Phenylephrine hydrochloride 86Phenylethylalcohol 1302-Phenylethyl glycoside 67o -Phenylphenol 114Phenylpropanolamine hydrochloride 86Phenytoin 89, 92Phloxine 57Phosphoenolpyruvic acid monopotassium salt 52Phthalic acid 120Phylloquinone 8, 12Pilocarpine chloride 60Pipemidic acid 102Pirimicarb 114Piromidic acid 100, 102, 103Piroxicam 87Plasmid pBR322 38Poly (A) 37Polydextrose II 29Potassium guaiacol sulfonate 82Prednisolone 71, 73, 74Prednisolone acetate 72, 73, 74Prednisolone succinate 73Prednisone 71, 72, 74Prenylamine lactate 91, 92Primidone 89Procainamide hydrochloride 96 Procaine hydrochloride 93Prochlorperazine maleate 89Profenamine hydrochloride 89Progesterone 71, 76Proline 39L-Proline 39, 40Promazine hydrochloride 89Prometon 112, 113Prometryn 112, 113Propazine 112, 113

INDEX

137

Propham 113Propicillin potassium 109Propionaldehyde 2,4-DNPH 116Propionamide 125Propionic acid 52Propionyl-CoA 18p -Propoxybenzoic acid 118, 119Propranolol hydrochloride 92, 127, 130n -Propyl gallate (PG) 55, 56Propyl p-hydroxybenzoate 55, 59Proscillaridin A 79Pullulan P-5 (MW 5,800) 30Pullulan P-20 (MW 23,700) 30Pullulan P-50 (MW 48,000) 30Pullulan P-800 (MW 853,000) 30Pyrene 117Pyridoxal hydrochloride 16, 17Pyridoxine hydrochloride 13, 14, 15, 16, 17,

86Co(II)-1-(2-Pyridylazo)-2-naphthol 124Fe(III)-1-(2-Pyridylazo)-2-naphthol 124Ni(II)-1-(2-Pyridylazo)-2-naphthol 124Zn(II)-1-(2-Pyridylazo)-2-naphthol 124Pyrimethamine 100Pyrogallol 120Pyrroloquinoline quinone (PQQ) 15Pyruvic acid 52

QQuercetin 64, 65Quercitrin 65Quinidine 60, 92Quinine 608-Quinolinol 124

RRaffinose 23Rebaudioside A 59Resibufogenin 80Resorcinol 18, 108, 118α-Resorcylic acid 118β-Resorcylic acid 118γ-Resorcylic acid 118Retinol 8Retinol acetate 8Retinol palmitate 8Rhamnose 20Ribitol 21Riboflavin 13, 14, 15, 16, 17,

18Ribonuclease A 45, 46Rose Bengale 57Rutin 65

SSaccharin sodium 54, 81Saikosaponin a 63

Saikosaponin b2 63Saikosaponin c 63Saikosaponin d 63Salicin 61Salicylic acid 96, 118Saponin 62Sccinyl-CoA 18Scopolamine bromide 60Sebuthylazine 112, 113Sennoside A 67Sennoside B 67Serine 39L-Serine 39, 40Siduron 112Simazine 112, 113β-Sitosterol 78Sodium decylbenzenesulfonate 115Sodium dodecylbenzenesulfonate 115Sodium tetradecylbenzenesulfonate 115Sodium tridecylbenzenesulfonate 115Sodium undecylbenzenesulfonate 115Sorbic acid 54Sorbitol 21, 22Sparteine sulfate 60Stachyose 23Stevioside 59Stigmasterol 78G-Strophanthin 61, 79Substance P 42Succinic acid 52Sucralose 22Sucrose 20, 22, 23Sulfachloropyridazine 101Sulfadiazine 100, 101, 102Sulfadimethoxine 100, 101Sulfadimidine 100, 101, 102Sulfadoxine 101Sulfaguanidine 100, 101Sulfamerazine 100, 101, 102Sulfamethazine 100, 101, 102Sulfamethizole 100, 101Sulfamethoxazole 101, 102Sulfamethoxypyridazine 100, 101Sulfamonomethoxine 100, 101, 102Sulfanilamide 100, 101Sulfapyridine 100, 101Sulfaquinoxaline 100, 101Sulfathiazole 100, 101, 102Sulfisomidine 100, 101Sulfisoxazole 101Sulindac 87Sunset Yellow FCF 57Swep 113

TTartaric acid 52Tartrazine 57Taurine 39,405'-TDP 35Terbumeton 112,113Terbuthylazine 112,113


138

Terbutryn 112,113Terephthalic acid 120o-Terphenyl 123m-Terphenyl 123p-Terphenyl 123Testosterone 75, 76Testosterone enanthate 75, 76Testosterone propionate 75, 76Tetracaine hydrochloride 93Tetracycline hydrochloride 102, 110Tetrahydrozoline 84Theobromine 61, 97Theophylline 61, 96, 97Thiabendazole 114Thiamine hydrochloride 13, 14, 17, 81Thiamphenicol 100Thiophanate-methyl 112Thioridazine hydrochloride 89Thiram 111, 112Threonine 39L-Threonine 39, 40Δ-Threonine 39Thymidine 34, 35Thymine 345'-TMP 35, 36α-Tocopherol 8, 10, 11, 12β-Tocopherol 10, 11, 12γ-Tocopherol 10, 11, 12δ-Tocopherol 8, 10, 11, 12α-Tocopherol acetate 8, 12Tolmetin sodium 87p -Tolualdehyde 2,4-DNPH 116Triacylglycerol 53Triamcinolone 73Triamcinolone acetonide 72, 73, 742,4,6-Trichlorophenol 121Triethanolamine 124Trifluoperazine hydrochloride 892,2,2-Trifluoro-1-(9-anthryl)-ethanol 126Triglycine 49Trigonelline 612,4,5-Trihydroxybutyrophenone(THBP) 55, 56Trimethoprim 100, 101O -Trimethyl-β-cyclodextrin 27, 282,4,6-Trinitrophenol 121Triphenylene 117Triton X-100 115, 116Troger's base 130Tryptic digest of synthetic pCDD 88 40Tryptic digest of synthetic pCDD 126 40Tryptophan 39L-Tryptophan 395'-TTP 35Tyrosine 39L-Tyrosine 39, 40

U5'-UDP 35UDP-N-acetyl-D-glucosamine 37UDP-D-glucose 375'-UMP 35, 36

Uracil 34, 35, 94, 96, 124Urea 17, 125Uric acid 97, 98Uridine 34, 355'-UTP 35

Vn -Valeraldehyde 2,4-DNPH 116Valine 39L-Valine 39, 40Vancomycin hydrochloride 110[Arg8]-Vasopressin 43Verapamil hydrochloride 91, 92Vitamin A 8Vitamin A palmitate 8Vitamin B1 13, 14, 17Vitamin B2 (Flavin mononucleotide) 13, 14, 18Vitamin B2 (Riboflavin) 13, 14, 15, 16, 17,

18Vitamin B6 (Pyridoxal hydrochloride) 17Vitamin B6 (Pyridoxine hydrochloride) 13, 14, 15, 16, 17,

86Vitamin B12 13, 14, 15, 16Vitamin B13 15, 35Vitamin C 13, 15, 16, 17, 19Vitamin D2 8, 9, 10Vitamin D3 8, 9, 10Vitamin E 8Vitamin H 14, 15Vitamin K1 8, 12Vitamin K2 8, 12Vitamin K3 8

XXanthine 16, 34, 97Xanthosine 34, 35Xylitol 21, 22Xylose 20

INDEX

139

AUTHORIZED DISTRIBUTOR

M E M O

3 ymc application data - mz-at.de · 5 index 医薬品、生体関連物質 drugs, metabolites...

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