5. (cytolytic reaction)

(, ) ,

1) (Hemolytic reaction)

() ( hemolysin:)

++ ->

2) (Bacteriolytic reaction) () ( bacteriolysin)

+ + ->

Gram phospholipid -- (Pfeiffer)

Gram

(bacteriolysin, hemolysin) 56 30

6. (complement fixation reaction)

- -

1

2

, influenza, virus , , Rickettsia, ,

.() 56 30

7. (Absorption reaction)

()

8. ()(Inhibition reaction)

2 1

anti-hCG Anti-hCG

hCG Latex

virus

influenza virus

9. *

(Immunoassay)

(RIA: Radioimmunoassay)

(ELISA: Enzyme-linked immunosorbent assay, EIA)

(: Immunofluorescence)

Immuno-Blot

1)

125I

-

hapten

insulin

hormone

,

,

(solid phase method) plastic

Bead

Bead

1966 ELISA (peroxidase, alkaline phosphatase, -D-galactosidase) ,

()

filter

( ) (fluorescent antibody)

(anti-human -globulin)

Immuno-Blot

()

SDS polyacrylamide

Nitrocellulose

1-

2-

,

(autoradiography), (, )

10.

Turbidimetry

Nephelometry

(Flowcytometry)

Turbidimetry, nephelometry

* Turbidimetry ()

/

Immunoglobulin, CRP, ASO, Complement

* Nephelometry ()

, ,

Beer(Lambert-Bouquers)

: 0.2 ~ 1.0 (1.0 )

A=abc=log(100/%T)=2-log%T A(absorbance): a: (molar absorptivity) b: light path c: %T:

(monochromatic radiation)

, , , ,

, ,

DNA Reticulocyte

. What is Flow Cytometer ?

Flow () + Cyto ()

() (Particle) (Cell)

(sensing area)

Signal

.

Flowcytometer []

+ Meter ()

Consist of the FCM

1. Fluidic System

(Laser )

laser

+ - Charged Plates

FSC detector

Fluorescence detector

Sorting Mechanism

Light signal

Electronic pulse

Channel Number

Data stored by comuter

ADC

Summary

Thank you! ^^

http://blog.goodbyecrutches.com/GoodbyeCrutches-blog/bid/128865/Top-5-Vacation-Destinations-while-on-Crutches