省產石花菜萃取物對細胞生長抑制的探討

中文摘要 本研究之目的乃在探討省產石花對於細胞之生長是否具有抑制作用，並且觀

察其生長抑制之作用是否具有選擇性，同時並探討其是否因誘導細胞程序化凋亡 (apoptosis) 之故。石花菜分別以 PBS 及 methanol 萃取之，並依照一般食用方式製備石花凍，經冷凍乾燥後溶解於 DMSO 中而後偵測不同之萃取物對Hepa1(Murine hepatoma cell) 、 HL-60 Human promyelocytic leukemia cell) 二株癌細胞以及正常 NIH3T3 Murine embryo fibroblast cell) 細胞生長的影響。細胞數目使用 Coulter counter 計算之，細胞增殖測試則使用 MTS assay kit 偵測，並以 DNA 電泳以及 Annexin V-FITC 螢光染色法觀察石花菜萃取物是否可誘導細胞產生 apoptosis 的現象。結果顯示，石花菜之 PBS- soluble extract對 3 種細胞株之生長及增殖都沒有抑制的效果 ( p>0.05) ；但其 methanol-soluble extract 對 Hepa1 及 NIH3T3 細胞之生長及增殖有抑制的作用 (p<0.05) ；且 DMSO-soluble extract 可抑制 3 種細胞株之生長及增殖的作用 (p<0.05) 。螢光染色及 DNA 電泳分析結果顯示， Hepa1 及 NIH3T3 細胞經 methanol-soluble extract 處理， 3 種細胞株經 DMSO-soluble extract 處理後，都有 annexin V positive 反應，細胞之 DNA 亦有斷片之產生，顯示石花菜萃取物會誘導細胞apoptosis 的發生。總言之，石花菜之 methanol- 及 DMSO-soluble extract 具有抑制細胞生長的果，且其生長抑制作用可能由於其可誘導細胞 apoptosis 的發生，但是此作用並不具有選擇性。

Antiproliferation effects of Gelidium amansii extracts on cultured cells

英文摘要 The objective of this study was to investigate the effect of Gelidium amansii on the

cell growth and the potential role of apoptosis on the growth inhibition was also explored. Two lines of cancer cells, murine hepatoma cell (Hepa1) and human promeyolitic leukemia cell (HL-60), and one normal cell lines, murine embryo fibroblast cell (NIH3T3) were used in this study. The cells were treated with PBS-, methanol-, or DMSO extracts of Gelidium amansii, and the cell growth and the cell proliferation were measured. The results indicate the PBS - soluble extract did not have the effects on the all lines of cells, whereas methanol-soluble extract had the inhibitory effect on the Hepa1 and NIH3T3 cells (p<0.05), and DMSO- soluble extract had inhibitory effects on all lines cells (p<0.05). The methanol-soluble extract treated Hepa1 and NIH3T3 cells, and all lines of cells treated with DMSO-soluble fraction of the Gelidium amansii showed annexin V positive response, and had fragmented DNA ladders on agarose gels indicating that the cells underwent apoptosis. Gelidium amansii possesse the antiproliferation effect of cultured cells and this may due to the induction of the apoptosis pathway, this inhibitory effect did not show cell specificity.