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BIOTEK

TANA

BIOTEKMOLE

2

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•REPLIKASI DNA

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•Replikasi DNA

• Proses perbanyakan DNA

• DNA ------  2 DNA

• Replikasi melibakan s!a! mekanisme yan"erine"rasi #ari beberapa en$im #an proein

• DNA polimerase I% II #an III #an RNApolimerase

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•Aturan Dasar Replikasi

&' Replikasi a#ala() s!a! proses yan"semi*on*er+ai+e% sa! ara(

2' Replikasi memp!nyai ara()• Berm!la pa#a s!a! iik pa#a kromosom%

berlan"s!n" ke ke#!a ara(% ,o "ro,in" poin o.ork/' Per"erakan ke#!a .ork men0a!(men"(asilkan s!a! ben!k "elemb!n"

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1' Replikasi #ari ke#!a sran# berlan"s!n" #en"

pemas!kan NTP #en"an ara( /-1/

3' Pa#a sin"le sran# i#ak berlan"s!n" se*arasim!lan% #en"an sa! DNA polimerase' Replikse*ara s(or #is*onino!s p!lse

' Replikasi pa#a in"ka s(or .ra"men4 #im!l#en"an erben!knya s(or 5e"men4 #ari RNseba"ai primer !n!k DNA polimerase% kekosoaa! "ap yan" er0a#i #iisi ole( DNA 6Oka$aki

7ra"men8

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REPLIKASI INVIVOMEMBUTUHKAN BANYAK ENIM

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ISOL

DN

ByPresenterMe!ia

http://www.presentermedia.com/mspp.htmlhttp://www.presentermedia.com/mspp.html

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&99 µl B!:er ;TAB&99mM Tris-

5enri.!"e &9'99rpm 3o;  meni

Ca*!m & 29

meni

@ #

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CI5UALI5A5I DNA

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CENTRAL DOGMA GENETIKA MOLEKUL

 Prinsip Ekspresi Gen

DNA

(gen)

RNA

Protein Ciri/Sift

(!enotipe)

Trns"ription

Trns#tion

Tinggi Tn$n

%ent&k %

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Se"r U$&$ $"$ teknik %iot

• I*entifiksi gen *ri spe"ies #in +n

"iri/sift tertent& (+ng *iinginkn)• At& $o*ifiksi gen +ng s&*, *

Mnipsi Gen

• Intro*&ksi gen p* orgnis$e• Teknikn+ *ise-&t Transformasi • Pro*&kn+ Organisme Trangenik 

Intro*&ksi Gen

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TANAMAN

TRANSGENIK 

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Nt&r# Infe"tion Pro"ess Is Co$p#e.

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T,e Go#*en Ri"e Stor+• Pro-#e$ kese,tn *efisiensi it A

Men+e--kn ke-&tn Le-i, *ri 011 '&t nk $en*erit

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2Crotene Pt,3+ in P#nts

IPP

Gern+#gern+# *ip,osp,te

P,+toene

L+"opene

  2"rotene

(it$in A pre"&rsor)

P,+toene s+nt,se

P,+toene *est&rse

L+"opene2-et2"+"#se

42"rotene *est&rse 

 Problem:

 Rice lacksthese enzymes

 Normal 

Vitamin A

“Deficient” 

 Rice

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T,e Go#*en Ri"e So#&tion

IPP

Gern+#gern+# *ip,osp,te

P,+toene

L+"opene

  2"rotene

(it$in A pre"&rsor)

P,+toene s+nt,se

P,+toene *est&rse

L+"opene2-et2"+"#se

42"rotene *est&rse 

Dffo*i# gene

Sing#e -"teri# gene5

perfor$s -ot, f&n"tions

Dffo*i# gene

 2Crotene Pt,3+ Genes A**e*

6it$in A

Pt,3+

is "o$p#eten* f&n"tion#

Golen

 Rice

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L- Testing T,e Trnsgeni"s

Inse"t Resistn"e

Trnsgene7

 !t"to#in $rotein

Co#* To#ern"e

Trnsgene7

%!& transcri$tion factors

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S#t To#ernt Mer"&r+ Resistn"e

More Mo*ern E.$p#es

Trnsgene7

Glyo#ylase ' 

Trnsgene7

 (erc)ric ion re)ctase

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T,e Ne.t Test Is T,e !ie#*

Non2trnsgen

Trnsgeni"s

 *erbicie Resistance