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adBMP-2 CMV IgG - + + anti-integrin α5 + - - - + + + - - - + - - + - - - - + + - S Figure 5 ALP/S29 0. 5 1. 0 1. 5 ALP S5. Effects of integrin α5 on osteogenesis. hBMSC were tranduced with control adenovirus (CMV) or adBMP-2 to induce osteogenic differentiation in vitro. Transduced cells were then incubated in the presence or absence of either control IgG antibodies or blocking antibodies to integrin α5. The relative level of osteogenesis in the treated cells was determined by measuring the expression of ALP mRNA.

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ALP. 1.5. 1.0. ALP/S29. 0.5. CMV. +. -. +. -. +. -. adBMP-2. -. +. -. +. -. +. IgG. -. -. +. +. -. -. -. -. -. -. +. +. anti-integrin α 5. - PowerPoint PPT Presentation

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Page 1: adBMP-2

adBMP-2CMV

IgG

- ++

anti-integrin α5

+- -- ++ +--

- +- -+ -- -- ++-

S Figure 5

ALP/S29

0.5

1.0

1.5

ALP

S5. Effects of integrin α5 on osteogenesis. hBMSC were tranduced with control adenovirus (CMV) or adBMP-2 to induce osteogenic differentiation in vitro. Transduced cells were then incubated in the presence or absence of either control IgG antibodies or blocking antibodies to integrin α5. The relative level of osteogenesis in the treated cells was determined by measuring the expression of ALP mRNA.

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