amino acid metabolism 生化教研室:牛永东. 生物化学的学习方法...
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Amino Acid Metabolism
生化教研室:牛永东
生物化学的学习方法 特点一:与数理化不同,尚未进入定量科
学的阶段,还处在定性科学阶段。因此不可能通过公式或定理推出一个准确的结论;
特点二: 是没有绝对,几乎所有的结论都可以被一些例外打破(生物多样性)。
一般性结论:生物化学的学习应以概念为主 --- 以记忆为主,在记忆的基础上加以理解。
【目的与要求】
• 掌握脱氨基作用、氨的来源、去路,氨的转运……
• 掌握尿素合成的部位和全过程
• 掌握一碳单位的概念、来源、载体和功能
• 需要掌握的概念:
必需氨基酸、蛋白质的互补作用、氨基酸库、联合脱氨基作用 .……
Metabolism
• consists of both catabolic and anabolic processes
• Catabolism comprises all processes, in which complex molecules are broken down to simple ones
• Anabolism means any constructive metabolic process by which organisms convert substances into other components required for the organism's chemical architecture
Introduction • Amino acids (AAs) are the building blocks of prote
ins (precursors for proteins) (物质代谢 )• Energy metabolites (17.9KJ/g Pr) : When degrad
ed, amino acids produce glucose/carbohydrates and ketone bodies (能量代谢)
• Precursors for many other biological N-containing compounds , Involved as direct neurotransmitters or as precursors to neurotransmitters, eg. (信息分子代谢)
- Tyrosine gives DOPA and dopamine - Precursors to peptide hormones and thyroid ho
rmone - Precursors to histamine, NAD and other compo
unds of biological importance
some major biological functions
• Detoxification of drugs, chemicals and metabolic
by-products
* Excess dietary AAs are neither stored nor excrete
d. Rather, they are converted to common metab
olic intermediates
outline
1. The nutrition of protein
2. The digestion 、 absorption and
putrefaction of protein
3. The general metabolism of AA
4. Metabolism of ammonia
5. Single AA metabolism
Section 1 The nutrition of protein
• Nitrogen balance
• The requirements
• Classification of amino acids
Nitrogen balance• Zero or total nitrogen balance: the intake = the excretion (adult) Amount of nitrogen intake is equal to the
amount of nitrogen excreted is zero or total nitrogen balance
• Positive nitrogen balance: the intake > the excretion during pregnancy, infancy, childhood and
recovery from severe illness or surgery • Negative nitrogen balance: the intake < the excretion following severe trauma, surgery or
infections. Prolonged periods of negative balance are dangerous and fatal if the loss of body protein reaches about one-third of the total body protein
The requirements
• The requirements of protein for the health: the minimal requirement of protein is 30~50 gram for the adult
• Advice: 80 gram/day (中国营养学会 ) ? ? ?
• non-essential amino acids - can be synthesized by an organism - usually are prepared from precursors in 1-2
steps • Essential amino acids *** - cannot be made endogenously
- must be supplied in diet
eg. Leu, Phe…..
Classification of amino acids
Nonessential EssentialAlanine Arginine*
Asparagine Histidine *Aspartate ValineCysteine Lysine
Glutamate IsoleucineGlutamine Leucine
Glycine Phenylalanine
Proline MethionineSerine Threonine
Tyrosine Tyrptophan *The amino acids Arg, His are considered “conditionally essential” for reasons not directly related to lack of synthesis and they are essential for growth only
借来一两本淡色书
nutritional value
• Legumes(豆类 ) poor in Trp, but rich in Lys;
Cereals (谷类谷类 ) poor in Lys, but rich in Trp
• Mutual complementation of amino acids
• Protein deficiency-kwashiorkor, generalized ed
ema and liver enlargement, abdomen bulged
• Suggestion: the combined-action of protein
in diet
Section 2
The digestion 、 absorption
and putrefaction of protein
• Proteins are generally too large to be absorbed by the intestine and therefore must be hydrolyzed to the amino acids
• The proteolytic enzymes responsible for hydrolysis are produced by three different organs: the stomach 、 pancreas and small intestine (the major organ)
Digestive Tract of protein
Stomach
• HCl (parietal cells ) and Pepsinogen (chief cells )
• The pH of gastric juice is around 1.0. Food is retained in the stomach for 2-4 hrs
• HCl kills microorganisms, denatures proteins, and provides an acid environment for the action of pepsin
• Autocatalysis: pepsinogen is converted to active pepsin(Pepsin A) by HCl
• Pepsin coagulates milk in presence of Ca2+ ions
Pancreas and small intestine
• Endopeptidase (pancreas) Trypsin: carbonyl of arg and lys Chymotrypsin: carbonyl of Trp, Tyr, Phe, Met,
Leu Elastase: carbonyl of Ala, Gly, Ser Exopeptidase (pancreas) Carboxypeptidase A:amine side of Ala, Ile, Leu,
Val Carboxypeptidase B: amine side of Arg, lys • Aminopeptidase (small intestine): cleaves N-terminal residue of oligopeptidaes Dipeptidase (small intestine)
NH2 CH C NH CH- -
R1
O
R2
-NH CH C NH CH- -
R3
O
R4
-NH CH C NH CH
R5
O
R6
COOH
aminopeptidase
endopeptidase
carboxypepidase
NH2 CH C NH CH
O
R"
COOH
R'Amino acids +
Amino acids
dipeptidase
1/3
95%
absorption
• There is little absorption from the stomach
apart from short- and medium- chain fatty
acids and ethanol
• Under normal circumstances, the dietary
proteins are almost completely digested to
their constituent amino acids, and these end
products of protein digestion are rapidly
absorbed from the intestine into the portal
blood
• Amino acids are transported through the brush border by the carrier protein and it is an active transport
1. The classification of carrier protein: aciditic; basic; neutral and gly-carrier 2. -glutamyl cycle (-谷氨酰基循环 )3. The bi-and tri- peptidase carrier system in th
e intestinal mucosa cell
ADP+Pi
ATP
K+
K+ Na+
Na+
Na+Amino acids
Na+Amino acids
outer
Member
innner
K+-ATPase
Carrier protein
The mechanism of AA’s absorption
intestine
Cys-Gly
-gltamyl cyclotransfera
se
5oxoprolinase
GSH synthetase
GCS synthetase membrane
-glutamyl cycle
Gly
Cys
peptidase
GSH
-GGT
|||||||||||
|||||||||||
AA
COOHCHR
H2N
-GlnCOOHCHNH2
CH2
CH2C
O
HN CH
COOH
R
COOHCHR
H2N
ATP
ATP
ATP
ADP+Pi
Glu ADP+Pi
-glutamylcysteineADP+Pi
• Putrefaction: the process of decay of un-digestive and un-absorbed protein and the products by bacterial, fungal in the intestine 5 %
Putrefaction
1.Amines(胺 ):
–CO2
Amines
RCH2NH2
AA
CH COOHR
NH2
False neurotransmitter are similar with neurotransmitter
Ammonia
diffuse blood
intestine bacteria
+ NH3CH2 COOHR
A. some amino acids are degraded by the in the intestine bacteria
AA
CH COOHR
NH2
2. Ammonia( 氨 )-1:
C=O
NH2
NH2
urea
Enter instein
Urea enzyme CO2
+2NH3
diffuse blood
ammoniaUrea in blood
B. urea from the blood to the intestine
with resultant increased diffusion of
NH3 into the intestinal
2. Ammonia( 氨 )-2:
3. The other toxic material:
phenol, indole, sulfureted hydrogen……
Section 4 The general metabolism of AA
• Protein and amino acid turnover• Degradation of Amino Acids ( Fate
of amino group )• The metabolism of α-ketoacid
Body protein
Amino acids
Protein degradation
Protein turnover
Reutilization for new protein synthesis
Protein and amino acid turnover
1-2%
75-80%
T1/2 ?
(half time)
introduction
1. Proteins constantly being synthesized
and degraded - need constant supply of
amino acids
- need to degrade to protect from
abnormal proteins
- regulate cellular processes
2. Degraded by ubiquitin label - Ubiquitin binds lysine side chain - Targets for hydrolysis by proteosomes in cy
tosol and nucleus - ATP required 3. Degraded by the protease and the peptidase i
n the Lysosome - non- ATP required - the hydrolysis-selective are bad
The ubiquitin degradation pathway
E1-E1-S-S-
E1-E1-SHSH
E2-E2-S-S-
E1-E1-SHSH
E2-E2-SHSH
E2-E2-SHSH
ATP AMP+PPiATP AMP+PPi EE33
ubiquitinational protubiquitinational proteinein
ATPATP
26S Proteasom26S Proteasomee
20S 20S ProteasomProteasomee
ATPATP
19S regulate substra19S regulate substratete
E1 : activiting enzyme E2 : carrier protein E3 : ligase
( ubiquitin )
阿龙 - 西查诺瓦
Amino acid pool
Diet protein
Tissue protein
Carbohydrate
(glucose)
transamination
Nonprotein nitrogen derivatives
Amino nitrogen in glutamate
deamination
NH3
Urea
Acetyl-CoA
Citric
Acid
Cycle
CO2
Ketone dodies
Overview of the protein metabolism
R group
aH N3+
H
Amino group
Carboxylic group
COO-
Degradation of Amino Acids - Reactions in amino acid
metabolismAmino acid
• Free amino acids are metabolized in identical ways, regardless of whether they are released from dietary or intracellular proteins
• The metabolism of the resulting amino group and nitrogen excretion are a central part of nitrogen metabolism
introduction
FATE OF AMINO GROUP
DEAMINATION A. Transamination
B. Oxidative deamination
C. purine nucleotide cycle
A. Transamination
• Transamination by Aminotransferase (transaminase)
• always involve PLP coenzyme (pyridoxal phosphate)
• reaction goes via a Schiff’s base intermediate
• all transaminase reactions are reversible
Aminotransferases• Aminotransferases can have specificity for the al
pha-keto acid or the amino acid• Aminotransferases exist for all amino acids exce
pt proline and lysine• The most common compounds involved as a do
nor/acceptor pair in transamination reactions are glutamate and a-ketoglutarate, which participate in reactions with many different aminotransferases
to an alpha-keto acid alpha-amino acid
Transamination
aminotransferases
*** ALT and AST are components of a "liver function test". Levels increase with damage to liver (cirrhosis, hepatitis) or muscle (trauma)
Glu+pyruvate glutamate-pyruvate aminotransferase GPT, ALT
-Ketoglutarate+Ala
Glu+Oxaloacetate Glutamic oxaloacetictransaminase
GOT, AST -Ketoglutarate+Asp
((草酰乙酸草酰乙酸))
(( αα--酮戊二酸酮戊二酸))((丙酮酸丙酮酸))
The mechanism of transamination
+N
CH3OH
CH2OPO3H2
CO
H
PLP
–H2O
+H2O
Schiff’s base
AA
HOOC C NH2
H
R1
Schiff’s base isomer
N
CH3OH
CH2OPO3H2
CH2NH2
PMP( 磷酸吡哆胺 )
–H2O
+H2O
Molecule rearrange
-ketoacid
+HOOC C O
R1
Transamination
• Interconversion of amino acids
• Collection of N as glu
• Provision of C-skeletons for catabolism
B. Oxidative Deamination• L-glutamate dehydrogenase (in mitochondria)
Glu + NAD+ (or NADP+) + H2O NH4+ + a-ketogl
utarate + NAD(P)H +H+
Requires NAD+ or NADP + as a cofactor
Plays a central role in AA metabolism ?
It is inhibited by GTP and ATP, and activated by GDP and ADP
urea cycle
?
Combined Deamination
?
Combined deamination =
Transamination + Oxidative Deamination
The major pathway !!!
AA
-Ketoglutarate
-Keto acid
Asp
Oxaloacetatemalate fumarat
e
IMP
AMP
H2O
NH3
C. purine nucleotide cycle
aminotransferases AST
The metabolism of α-ketoacid
• Biosynthesis of nonessential amino acids
TCA cycle member + amino acid α-keto acid + nonessential amino acid
• A source of energy (10%) ( CO2+H2O )• Glucogenesis and ketogenesis
* Classification of amino acids
* glucogenic amino acid : are converted into either pyruvate or one of the citric acid cycle intermediates
(a-ketoglutarate, succinyl CoA, fumarate or malate)
* ketogenic amino acid: will be deaminated via Acetylc-CoA and thus can be made into a ketone body. such as: Leucine and lysine
* glucogenic and ketogenic amino acid: isoleucine, phenylalanine, tryptophan and tyrosine, threonine
Ammonia is toxic, so cells
need to get rid of it…..
1. amino acids degradation
RCCO2H2NH2 RCHO+NH3
MAO
Monoamine oxidase
*** Sources
2. glutamine (glutaminase, kidney)3. catabolism from bacteria in intestine (two) 4. purine and pyrimidine catabolism
Metabolism of ammonia
• Fix ammonia onto glutamate to form glutamine and use as a transport mechanism
• Transport ammonia by alanine-glucose cycle and Gln regeneration
• Excrete nitrogenous waste through urea cycle
Transport of ammonia
• alaninie - glucose cycle *
• regenerate Gln
Alanine-Glucose cycle
In the liver alanine transaminase tranfers the ammonia to α-KG and regenerates pyruvate. The pyruvate can then be diverted into gluconeogenesis. This process is refered to as the glucos
e-alanine cycle
Gln regeneration
**** Urea synthesis
• Synthesis in liver (Mitochondria and cytosol)
• Excretion via kidney
• To convert ammonia to urea for final excretion
Ornithine cycle
The urea cycle : 1932 by Hans Krebs and Kurt Henseleit as the first metabolic cycle eluc
idated
Krebs-henseleit cycle
arginase
Mitochondria
cytosol
OCT
(瓜氨酸)
(鸟氨酸)
1
2
3
4
5
UREA CYCLE (liver)
1. Overall Reaction: NH3 + HCO3
– + aspartate + 3 ATP + H2O urea + fumarate + 2 ADP + 2 Pi + AMP + ppi
2. Requires 5 enzymes: 2 from mitochondria and 3 from cytosol
Regulation of urea cycle1.Mitochondrial carbamoyl phosphate syn
thetase I (CPS I) CPS I catalyzes the first committed step of th
e urea cycle CPS I is also an allosteric enzyme sensitive t
o activation by N-acetylglutamate ( AGA ) which is derived from glutamate and acetyl-CoA
Increased rate of AA degradation requires higher rate of urea synthesis
AA degradation ↑glutamate concentration → ↑synthesis of N-acetylglutamate ↑CPS I activity ↑urea cycle efficiency
2. All other urea cycle enzymes are
controlled by the concentrations of their
substrates
Deficiency in an E ↑(substrate)
↑rate of the deficient E
3. The intake of the protein in food
the intake ↑ ↑urea synthesis
• Hyperammononemia: ammonia intoxicat
ion - tremors, slurring of speech, and blurr
ing of vision, coma/death
• Cause by cirrhosis of the liver or genetic d
eficiencies
Hyper-ammonemia and the toxic of the ammonia
Section 5 Single AA metabolism
• Decarboxylation
some neurotransmitters’ precursors for the decarboxylation of AAs production– bioactive amines
L-Glu decarboxylase
– CO2
GABA
(CH2)2
COOH
CH2NH2
L-Glu
(CH2)2
COOH
CHNH2
COOH
-aminobutyric acid (GABA) Glutamine can be decarboxylated in a similar PLP-dependent fashion, outputting -aminobutyric acid (neurotransmitter, GABA)
Decarboxylase
-CO2
L-CysCOOH
CHNH2
CH2SH
taurine
CHNH2
CH2SO3H3 ( O )
COOH
CHNH2
CH2SO3H
Taurine L-cysteine can be decarboxylated and converted into outputted the taurine
– CO2
Histidine decar
boxylation
Histamine
CH2CH2NH2
NHN
L-Histidine
COOHCCH2
NH2NHN
H
Histamine Histidine can also be decarboxylated in a similar PLP-dependent fashion, outputting the Histamine
NH
CH2 CH2NH2OH
5-hydroxytryptophan ( 5-HT )
COOHCHCH2
NH2
NH
Tryptophan
– CO2
Hydroxylase decarboxylase
5-HT
Polyaminies: (putrescine,spermidine,spermi
ne)
CO2 is then cleaved off in a PLP-dependent
decarboxylation, resulting in the polyamini
es (such as: SAM, spermidine, spermine)
***** One carbon unit metabolism
• One carbon unit: some AA may turn into the group including one carbon in the AA metabolism
Such as: methl -CH3 甲基
methylene -CH2 亚甲基 / 甲烯基
methenyl -CH= 次甲基 / 甲炔基 formyl -CHO 甲酰基 formimino -CH=NH 亚氨甲基 But without CO2
One carbon unit metabolism
• Folic acid / folate is an essential vitamin, and as such it cannot be synthesized within the human body
• Folate itself is not an active cofactor; its doubly-reduced form, is tetrahydrofolate (THF)
• Tetrahydrofolate (THF) : the carrier of one carbon
accepts one carbon groups from amino acids
Folate is reduced first by dihydrofolate reductase (DHFR) into dihydrofolate (DHF), oxidizing an NADPH in the process. DHFR, again oxidizing an NADPH+H+/NADP+, can also reduce DHF into THF
5
10
( N5-CH3-FH4 )
N
NH
NH2 NH
N
CH3
CH2
NH RO
(Folate)
N
NH
NH2 NH
N
ON
R
( N5 , N10-CH2-FH4 )
5
10
THF – biosynthetic pathways• There are three important biosynthetic
precursors synthesized from THF : N5,N10-methylene-THF N5-methyl-THF N10-formyl-THF • N5,N10-methylene-THF acts in a central
processing role in the synthesis of all of these enzymes: in order to synthesize either of the other two, one must first produce N5,N10-methylene-THF
N5,N10 甲烯四氢叶酸
NADH
NAD+
NADPH
NADP+
N5,N10-methylene-THF
THF
THF
NAD+
NADH
Glycinecataboase
Serine
Glycine
CO2+ NH3
Serine Hydroxymethyl Trans
ferase
N5-methyl-THF
N5,N10-methenyl-THF
N10-formyl-THF
H2O
biosynthesis
methionine
biosynthesis
thymidylate
biosynthesis
purinesTryptophan
Histidine
N5,N10 甲烯四氢叶酸
N5,N10 甲炔四氢叶酸
• Sulfanilamide (磺胺 ) is a compound that the human body can create. It is a close analog to PABA, and it has the effect of stopping folate synthesis in bacteria. There are a wide variety of “sulfa drugs ” ( 磺胺类药 ) based on PABA analogs such as sulfanilamide
• Trimethoprim (TMP) and pyrimethamin( 百炎净 ), on the other hand, are DHFR inhibitors. Because bacterial DHFR is structurally simpler than human DHFR, these two drugs have a more drastic effect on bacteria than they do on us
—S——S—
COOH
CHNH 2
CH2
COOH
CHNH 2
CH2
cysteine cystine
Met
(CH2)2
S-CH 3
CH-NH2
COOHCOOH
CHNH2
CH2SH
Metabolism of sulfur-containing amino acids
Methionine Catabolism
• The principal fates of methionine are incorporati
on into polypeptide chains(protein synthesis),
and use in the production of a-ketbutyrate and
cysteine via S-adenosyl methionine (SAM)
S-adenosyl methionine (SAM) • is a powerful methylating agent (in the methylating
gene regulation of DNA and RNA, It is constantly regenerated in a cyclical) with uses in many biochemical pathways
• formed from ATP and Met
+R
OO
OHOH
P
O
OH
P
O
O
OH
OH
OH
O
O
P
ATPMet
(CH2)2
S-CH3
CH-NH2
COOH
Adesine transferase
– PPi +Pi SAM
RO
CH2
OHOH
S+
(CH2)2
CH3
C-NH2
COOH
H
S-adenosylmethionine (SAM)• methyl group is donated to form several
products norepinephrine --> epinephrine gamma-butyric acid --> carnitine guanidinoacetate --> creatine ( 胍乙酸 )
SAM
PPi +Pi
RO
CH2
OHOH
S+
(CH2)2
CH3
C-NH2
COOH
H Met cycle
N5-CH3-FH4
FH4N5-CH3-FH4
methyl-transferase
Met(CH2)2
S-CH3
CH-NH2
COOH ATP
H2OA
SAHHS
(CH2)2
C-NH2
COOH
H
SAHH
FH4 is produced!!!
S-腺苷同型半胱氨酸同型半胱氨酸 OHOH
RH
RO
CH2S
(CH2)2
C-NH2
COOH
H
R-CH3
homocysteine methionine
s-adenosylmethionines-adenosylhomocysteine
vitamin B12 ATP
PPi + Pi
methyl group donated to biological substrate, e.g. norepinephrine
adenosine
H2O
N5-CH3-FH4 FH4
N5,N10 -methylene- FH4
NADH
NAD+
Carbon donors (serine, glycine) combine with THF
all 3 phosphate groups are lost!
S-腺苷同型半胱氨酸
同型半胱氨酸 Only one!
FH4 and Met cycle
Regulation of the Met metabolism
• If methionine and cysteine are present in adequate quantities, SAM accumulates and is a positive effector on cystathionine synthase( 胱硫醚合成酶 ), encouraging the production of cysteine and a-ketobutyrate
• If methionine is scarce, SAM will form only in small quantities, thus limiting cystathionine synthase activity. Under these conditions accumulated homocysteine is remethylated to methionine, using N5-methyl THF and other compounds as methyl donors
H2O
Creatine metabolism
( 胍乙酸 )
+ 2HCOOH
CHNH2
CH2SH
2
cysteine
– 2H
COOH
CHNH2
CH2
COOH
CHNH2
CH2—S——S—
cystine
Metabolism of Cystine and Cysteine
Cysteine Catabolism • The pathway is catalyzed by a liver desulfurase a
nd produces pyruvate and hydrogen sulfide (H2
S) • The enzyme sulfite oxidase uses O2 and H2O to c
onvert HSO3- to sulfate (SO4
-) , and H2O2. The resultant sulfate is used as a precursor for the formation of 3'-phosphoadenosine-5'-phosphosulfate, PAPS
Metabolism of aromatic amino acid
PhenylalaninePhenylalanine TyrosineTyrosine
CH2
CHNH2
COOH
CH2
CHNH2
COOH
OH
tryptophan
Phenylalanine hydroxyl
ase
THF DHF
NADPH+H+NADP+
phenylalanine
tyrosine
Phenylalanine metabolism
1. Catecholamine/melanin
Tyr metabolism
NH3+
|- CH2 - CH - COO-
CH2 - COO-
HO - - OH
Homogentisate
NH3+
|- CH2 - CH - COO-HO -
Tyr
Phe Hydroxylase
O ||- CH2 - C - COO-HO -
Tyraminotransferase
尿黑酸
对羟基丙酮酸
Tyr Catabolism
CH2 - COO-
HO - - OH Homogentisate
-OOC - CH = CH - C - CH2 - C - CH2 - COO-
|| || O O
cis
-OOC - CH = CH - C - CH2 - C - CH2 - COO-
|| || O O
trans
H ||-OOC - C = C - COO- + H3C - C - CH2 - COO-
H
O acetoacetatefumarate
尿黑酸
乙酰乙酸
延胡索酸
• Phenylketonuria (PKU) = lack of phenylalanine hydroxylase
- can’t hydroxylate phenylalanine to tyrosine
Phenylalanine Hydroxylase & PKU
[Phe] = 0.1 mM normally 1.2 mM in PKU
1 in 20,000 homozygous1 in 150 heterozygous
IQ study: 53 93
• People with phenylketonuria must avoid excess phenylalanine, but both tyrosine and phenylalanine are essential amino acids, so they shouldn’t exclude it completely or brain disorders with result
The Metabolism of
Branched Chain Amino
Acids
• Branched-chain amino acids (BCAAs): isoleucine, leucine and valine • The catabolism of all three BCAAs initiat
es in muscle and yields NADH and FADH2 which can be utilized for ATP generation
Isoleucine / Leucine / Valine
-keto acid
-keto-S-CoA
-ketoglutarate
glutamate
transamination
NAD+, CoASH
NADH, CO2
-keto acid dehydrogenase
(if Leu or Lys, onlythis path can be used)
ketogenesis
Proprionyl-CoA
Succinyl-CoA
gluconeogenesis
A metabolic block here
causes maple syrup urine
disease
丙酰 CoA
• Most nitrogen metabolism pathways are very complex
require many steps require input of ATP and NADPH are regulated by feedback inhibition
mechanisms (allosteric)