兽医应用免疫学 —— 兽医专业重要基础课 黄青云 唐满华 谢安新 编制...
Post on 02-Jan-2016
314 Views
Preview:
DESCRIPTION
TRANSCRIPT
-
2002.8
-
immune(Antigen)
-
18
-
1618 16171818JennerVaccine()Vaccination
-
1920 Pasteur 1880 1882
-
1890 Metchnikoff1883
1897Behring(1890)1891Erlich 1897
-
701963Wright,Deuglas
-
20 20
-
64
-
Immune system
-
bone marrow B 40% thymus T bursa of fabricius B
-
lymphnodeB25%T75%(M)(DC)
-
(spleen) T25% B65% (M)(DC)
-
membrane immune systemMIS IgA TBMDC
-
Harder gland TB
-
(lyphocyte) TBNKT 1. ()
-
2.(1)T(T cell recepter,TCR)1015TcT
-
TCR
-
TCR TCR V 50 57D 2J 70 13VJ,VDJ 5070=3 103 57 2 13=1103 + +N V-J V-D,D-J ~1015
-
(2)(Erythrocyte receptor,ER)
CD2T ET B
-
TE
-
3PHAConA
PHAConAT T TB T
-
TPHA
-
4T TCluster of differentiationCD
CD2 TcERCD3 5-6TcTCR/CD3 CD4 THTHTCR HIVCD8 TcTcTCR
-
3. T
CD TC CD3+ CD4- CD8+ IFN-
TS CD3+ CD4- CD8+ TSFTB m TDTHTH1 CD3+ CD4+ CD8- MIFIFN- TH2 CD3+ CD4+ CD8- IL-4IL-5 B
-
B
1
B
-
21Surface membrane immunoglobulingSmIg B SmIgM B SmIgM+SmIgD 107BBSmIgMSmIgDB105SmIg SmIgTCRB
-
(2) FcBFc
3C3b B C3b
-
BEEEEEEEABEEEEEEEACFcRFcC3bRC3b
-
2. B B1 B2 B1CD5+ B2CD5- Sm Ig Sm IgM Sm IgMSm IgDIgG Fc +C3b + TI Ag TD Ag IgM IgMIgGIgAIgEIgD +
-
((natural kill cell,NKC) 1. FcCD16Fc KIR KAR2. ADCC
-
- Kupffer A :
-
() Fc C3b Ia_ M Ia+ M
1. 2.monocyte Ia M
-
a2 C1 C2 C3 C4 C5 PDI H IL-1IL-6IL-8TNF E
-
O2
O2n-
H2O2OH-IO2
-
3.Ia+ MThTCR
-
4.
MTNF
M
-
T -M -(DC) -Lc DCMHC-
-
.1. IgG FcADCCIgE Fc2. IgE Fc3. C3b -() 1982sigel()
-
4TCRBCRSmIgM SmIgDCD cluster of differtiation,CD TCD2CD3THCD4TcCD8CD CD
-
MHCMHCMHC HLA
DP DQ DR B C A D HLA- HLA- HLA- 1000~1200kb 1000kb 1600~2000kb 3500~4000kb
MHCHLA
-
1MHC-
(1) (2) MHC-I TcTCR
-
2 MHC-
TH immuno- assodiated antigenIa
-
3MHC-C2C4BfTNF ()(ImmunoglobulingIg)BIg (SmIgM SmIgD) B(IgM IgG IgA IgE IgD)
-
1.Ig
-
2Ig
-
3Ig
-
()complement system
1 20C1C1qC1rC1s------C911
256OC60OC30min
-
3. C1 C3
-
C1 C3IgG1IgM IgGIgA Ca++ Mg++ C1C1 C3PAD Mg++ C3 C2+C4 C4b2a C3(Bb) C3PA C3b +C3a C5 C5b + C5a C 56789 8-17
-
C3aC5a
-
()
1.
-
2(1) interleukin,IL IL-1~IL-23 ,(2) (colony stimulating factor, CSF) G-CSF M-CSF EPO T
-
1interferonIFN
IFN- IFN- IFN- T :
-
Antigen + ()+ +
-
10000dal10dal AA5734dal AA
-
1.2. 3.4. 5. 6. 7. 8.
-
antigenic determinants,AD BAD TAD: () (): AA,, : 56AA,, 918AA: BCRSmIg APC MHC-/-TCR (Th) MHC-/ -TCR(Tc)
-
- - - - - ,() - ( ,) Th -TD Ag -TI Ag
-
- FHN, (Ab) VP1VP4 - (Ab )T - ADT MHC- - (super Ag) ADT ( M)
-
(O)Ag 170 60,50 , AF (H)Ag 60 60,(a.b.c) , (1.2.3) Ag - , - (KAg) (Vi Ag) , 100,K88K99 Ag -(,,,0.3-0.4% ) -(,,)
-
(1.4.5.12i.1.2) OAg 1.4.5.12 HAg i,1.2
-
() () () ()
-
(adjuvant):10: : AE: (): +: IL-2IL-12IL-18DNA
-
()(Freund,s incomplete adjuvantFIA)Ag++80 AgAg ()(Freund,s complete adjuvant FCA) : FIA+: FIA MTB
-
(immune response)
TDAgTIAg
-
TDAg
-
(T cell mediated immunity,TMI)
Ag pro-Tc Tc
Ag Th Ag Th1(TDTC)
-
Tc -MHC-TCR - - Th1 ThAg50()
-
-() - -()
-
(B cell mediated immunity,BMI) TDAg APC Th Th2
Bc (IgMIgG) (IgG) TDAg
-
TIAg Bc (IgM)(Antibody,AbImmunoglubulin,Ig)() 1(primary response)(3-72-3) IgM
-
BIg
-
2 () () AbAbIgG
-
clone selection theoryAb33
-
AbCH IgMIgGIgAIgE IgD2. Ag Ab2 Ab3. Ag AbAb AbAb Ab AbAb
-
4AbIgGAbIgGIgMAbIgGIgMAb IgGIgMIgAAbIgGIgMIgGIgGIgM
-
5AbAbAbmonoclone Ab,McAbMcAbBB
-
McAb
-
() MHC-() Ab
-
VitAVitCFeZnSn
-
B Ag T Ag Ab2-3Ag 3-5Ag
-
. -Ab2 Ab
-
-
-
M
-
IL-1IL-6TNF- (1) (2) C3
-
(3) TNFIgGIL-8 (4) IL-12 Th Th1 NK()
-
1()C3()LPSMTNF-IL-1IL-6IL-12
-
2 AbIgG
IgG
-
IgMIgGC1IgGSIgA
(SIgA) (IgG)
-
1M IFN IL-12 NK2IFN-MTc
-
IgGIgGIgMC1+
-
IFN-NKIFN-NK
-
-IgGIgGIgMIgG-TcFasLIFN-
-
IgE
-
allergy AgAgallergen:
-
1. AbTDTH 2. AbTDTH
-
1 IgE2 3 4
-
1. 2. 2030min 1. 2. 3.
-
1 IgMIgGMNK2 1 A B A
-
2 + Aa+ Aa- Aa+ Aa- ()
-
3. 4
-
Ab(IgGIgAIgM)(,)Ag+Ab (Ag-Ab)nAb Ag
-
AgAb +
-
1IgGIgAIgMAg 2. 3.
-
1 Authus2103Ag
-
Ag Th1(TDTH)Ag Th1 Ag 1Ag48-72h 2
-
1 - ()0.lml72h 0.lml15-18h
-
2 Th1 Th 24-96h () : (1) (2) :
-
3MHC- Th1 : (1) (2)
-
-TET-TT
-
ETCD2TCD2
-
TE
-
. + + + 1.TB2.T
-
PHA+TPHA =(+)/200100%
-
TPHA
-
3H3H-T PHA+T+3H-TdR PHA+T 3H-TdR 3H-TdR CPM 3H-TCPM SI=CPM/CPM
-
Ag+T+M T+ M M M M M MIMI=M/M100%
-
TMTTPHAConA+T MTT formazan 595nmOD
-
()1 2 3 4 5
-
1
-
2 PH 7.0 T 370C Ag+(Ab) Ag-Ab PH3 T600C
-
3Ag-Ab41 - - - -2-80C
-
2 0.85%NaCl 6%8% 340C560C 370C 30min
-
4PHPH6.08.0PH7.07.2PH6PHPHPH7.07.2PBS
-
0.85%NaCl + ()
-
1 Ascoli
-
2
-
3 Ag Ab
Ag
-
+ ()
-
1 2 ()
-
3+ +() ( )
-
()Ab
-
123 4
-
1 -NDV 911d 2448h
-NDV+ 911d
-
2 (FMDV) 47d FMDV+ 47d 3 FMDV (IB-RS-2) (CPU)
FMDV+ IB-RS-2 CPU
-
1.2.
-
1.F+ Ab AbF(FITC) AbF + Ag Ag- AbF Ag- AbFAbF
-
F F Ag Ag
AbF AbF
-
2.1 2
-
3. Ag+AbR Ag-AbR AgR+Ab AgR-Ab R
-
4 ()
-
--1.(1)2,()()
-
1234
-
2.+1%+123
-
3. ++(1) (2)4.()
-
:(+) -- ///// ::
-
1.+K88
-
2.1
+
-
2
()
-
3
-
4
-
5
-
3.
-
. , SRBC , ,
-
-5---
-
1 2
-
(/) 1 12 - 1 5 1 10 - 0.5ml 15 2 25 1 4550 1 ++ 0.51ml 1020 + ++ 0.51ml +
-
(/) 1 1 35 1 + 0.5ml 25 1 1 2 1520 2
-
(/) 25 2 15 30 1.5ml 10 2 20 2 4
-
(/) 30 2 45 1 O 1 50 1 1 70 3 O 1
-
(/) - 24 2- 4 2 2 2 O 2 () 2-4 2
-
1520 0.5ml/ 1) 0.5ml/ 2) 0.5ml/ (A) 2ml/ 1) 2) 1ml/ (A) 2ml/
-
1. 2.
-
1.2.
top related