©2000 timothy g. standish electrophoresis timothy g. standish, ph. d
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©2000 Timothy G. Standish
ElectrophoresisElectrophoresis
Timothy G. Standish, Ph. D.
SU
GA
R-P
HO
SP
HA
TE
BA
CK
BO
NE
H
P
O
HO
O
O
CH2
HOH
P
O
O
HO
O
O
CH2
H
P
O
OH
HO
O
O
CH2
NH2
N
N
N
N
O
O
NH2N
NH
N
N
N O
NH2
N
B A
S E
S
DDNNAA
OH
P
O
HO
O
O
CH2
HO
O
H 2N
NHN N
N H
H
P OH
O
O
CH2
OO
N
O
H 2N
NH
H2O
H OH
P
O
OH
O
O
CH2
CH 3
O
O
HNN
H2O
©2000 Timothy G. Standish
The Watson - Crick The Watson - Crick Model Of DNAModel Of DNA
Majorgroove
Minorgroove
A T
T AG C
C G
C GG C
T A
A T
G CT A
A TC G
--
-
-
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--
--
--
-
--
--
-
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--
--
--
-
-
©2000 Timothy G. Standish
Submarine Gel ElectrophoresisSubmarine Gel Electrophoresis
Gel BoxNegativeelectrode
Positiveelectrode
Sample to be loaded
Well Gel
©2000 Timothy G. Standish
Submarine Gel ElectrophoresisSubmarine Gel Electrophoresis
©2000 Timothy G. Standish
100 V
Submarine Gel ElectrophoresisSubmarine Gel Electrophoresis
+-DNA Migration
©2000 Timothy G. Standish
Gel ElectrophoresisGel Electrophoresis
Wells
©2000 Timothy G. Standish
Gel ElectrophoresisGel Electrophoresis
Wells
©2000 Timothy G. Standish
Gel ElectrophoresisGel Electrophoresis
+
-
Direction of
DNATravel
Wells
Small
Large
©2000 Timothy G. Standish
Electrophoresis of DyesElectrophoresis of Dyes
1 2 3 4 5
©2000 Timothy G. Standish
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