anti-uch l1
Post on 05-Feb-2016
43 Views
Preview:
DESCRIPTION
TRANSCRIPT
anti-UCH L1
anti-α-tubulin
H157
H358
SW40
3
COLO205
KM12
14
CaCO2
RIE-1
SMC
HUVEC
MCF7
Primary cells
Lung cancer cells
Small intestine epithelial cells
Colon cancer cells
Breast cancer cells
Supplementary Data (S 1.)
5h
71h
immediately
7h
b
a
control UCH-L1 RNAiuninfected
control UCH-L1 RNAiuninfected
Supplementary Data (S 2.)
anti UCH-L1
anti -tubulin
control UCH-L1 RNAi
wc sol. insol. wc sol. insol. wc sol. insol.
uninfected
control UCH-L1-myc
wc sol. insol. wc sol. insol. wc sol. insol.
uninfected
anti UCH-L1
anti -tubulin
UCH-L1-mycEndo UCH-L1
3 g 6 g
wc sol. insol. wc sol. insol. wc sol. insol.
Empty vector
anti UCH-L1
anti -tubulin
Flag-UCH-L1
Endo UCH-L1
Flag-UCH-L1
Supplementary Data (S 3.)
b
a
c
Supplementary Data (S 4.)
M1M2
M3
M1M2
M3
M1M2
M3
M1M2
M3
control UCH-L1 RNAicontrol UCH-L1 RNAi
control UCH-L1 RNAi
phase control UCH-L1 RNAi
G1 (M1) 46.59 47.69
S (M2) 22.18 20.60
G2/M (M3) 30.58 31.48
phase control UCH-L1 RNAi
G1 (M1) 54.73 56.30
S (M2) 17.04 16.15
G2/M (M3) 27.41 26.89
b
a
top related