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Achieve More Efficiency With Less Backpressure
Leverage Benefits on Any HPLC System
Utilize C18 and F5 Phases
Maximize Performance
Ascentis® Express 5 µm Columns with Fused-Core® ParticlesOutperforms Popular 3 µm and 5 µm Columns
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ORDER: 800-325-3010 TECHNICAL SERVICE: 800-325-5832
Ascentis Express 5 µm columns provide a new choice for improving the performance of traditional HPLC systems. Ascentis Express provides the benefits of high speed and high efficiencies without the concerns of smaller particle columns. Due to the high efficiencies at low backpressures, Ascentis Express 5 µm can benefit conventional HPLC users with no drawbacks.
Ascentis® Express 5 µmAchieve Faster Separations With No Backpressure Concerns
“With the addition of the 5 micron particle to the Ascentis Express product line, end users can now boost the performance of their legacy HPLC systems and effortlessly transfer methods to any site or CRO with no concerns of incompatibility. The Ascentis Express product line is scalable from UHPLC to legacy HPLC systems. Furthermore, these columns provide researchers interested in ultra-high resolution HPLC the ability to reach new levels of resolution through column coupling, due to their low back pressure.”
Features at a Glance
•High e�ciencies
•Low backpressures
•Same instrument – no change of methods or sample prep
•Faster method development
•C18 and F5 phases
•Rugged design
•Outperforms popular 3 μm and 5 μm columns
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0.5 µm
1.7 µm2.7 µm Solid Core
0.6 µm
3.3 µm~5 µm Solid Core
Small Diffusion PathThe new Fused-Core particle consists of a 3.3 µm solid core and a 0.6 µm porous shell. A major benefit of the Fused-Core particle is the small diffusion path (0.6 µm) compared to conventional fully porous particles. The short diffusion path reduces axial dispersion of solutes and minimizes peak broadening. In fact, Ascentis Express 5 µm columns are able to achieve greater speed and efficiency than any other 5 µm particle based column. This means that Ascentis Express 5 µm becomes the standard column for all of your 5 µm based methods.
Beyond the new standard column for all 5 µm based methods, Ascentis Express 5 µm is an excellent choice for bioanalytical LC/MS methods. The Ascentis Express 5 µm excels under high flow rates and the high throughput demands of these methods. Furthermore, the large particle format provides an extremely rugged HPLC column.
C18 and F5 (PFP) PhasesAvailable in 17 dimensions from 2 cm x 2.1 mm I.D. to 25 cm x 4.6 mm I.D. and two introductory phases, C18 and F5, the conversion of current methods is seamless.
Key benefits of C18 and F5 phases include:
•Great combination of phases for method development
•Orthogonal selectivity
•High probability for separation
•F5 retentive both in RP and HILIC Mobile Phase conditions
Ascentis Express Fused-Core Particles
Leverage Easy Implementation on Any System
2.7 µm 5 µm
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ORDER: 800-325-3010 TECHNICAL SERVICE: 800-325-5832
Maximize Performance Efficiencies With Fast Fused-Core
Fast HPLC with Shorter Diffusion Path
Fused-Core Particles Traditional Porous Particles
Consistent Bed Yields Sharper Peaks
Fused-Core Particles Traditional Porous Particles
Narrow Particle Size Distribution and Rugged Column Design
Fused-Core Particles Traditional Porous Particles
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0 2 4 6 8 10Min
Ascentis Express C18 5 μm, 15 cm x 4.6 mm ID 1.0 mL/min, N=23,422 for last peak, 80 Bar
0 10 20Min
2 4 6 8 12 14 16 18
Popular C18 5 μm, 15 cm x 4.6 mm ID 1.0 mL/min, N=13,633 for last peak, 72 Bar
Summary of Efficiency, Backpressure and Speed
Column Efficiency (N) Toluene Backpressure (PSI) Runtime
Ascentis Express “5” C18, 15 cm x 4.6 mm 23,000 1200 8 min
Popular 5 µm C18, 15 cm x 4.6 mm 14,000 1100 16 min
Twice the Efficiency, Same Backpressure
23,422
13,633
1,200 1,0800
5,000
10,000
15,000
20,000
25,000
Ascentis Express 5 µm Popular 5 µm
Efficiency (plates)
Backpressure (PSI)
3 μmTotally Porous
5 μmTotally Porous
5 μmFused-Core
0 100 200 300 400
360
Plates/Pressure in bar
Best Plates per Pressure
The 5 µm Ascentis Express Fused-Core particle has more than double the number of plates/pressure of the 5 µm totally porous particles and four times the number of plates/pressure of the 3 µm totally porous particles.
Outperform Popular 3 μm and 5 μm Columns
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ORDER: 800-325-3010 TECHNICAL SERVICE: 800-325-5832
Characteristics
Particle TypeShell
Thickness (µm)BET Surface Area (m2/g)
Average Pore Diameter (Å) Plates*
Pressure* (bar)
2.7 µm Ascentis Express Fused-Core 0.5 135 90 38,300 284
5 µm Ascentis Express Fused-Core 0.6 90 90 28,300 78
3 µm totally porous N/A 300 100 24,200 309
5 µm totally porous A N/A 300 100 14,600 100
5 µm totally porous B N/A 170 120 14,400 63
5 µm totally porous C N/A 450 100 15,300 120
* Plates and Pressure: reported for 1-chloro-4-nitrobenzene at the flow rate corresponding to the plate height minimum
Columns: 15 cm x 4.6 mm Mobile Phase: 50:50 ACN:water Temperature: 30 °C Injection: 1 μL Instrument: Agilent 1100 or Agilent 1200 with autosampler
10
100
1000
10000
1.00E+04 1.00E+05 1.00E+06
5 µm totally porous
3 µm totally porous
5 µm Fused-Core
t 0 (s
)
N (/)
Kinetic Plots of Various Particles Outperforms popular 3 μm and 5 μm columns under all conditions.
Compare Particle Attributes
Technical Conclusions
•Plates/Pressure: 5 µm Fused-Core particle has more than double the plates/pressure of 5 µm totally porous particles. – 4 times better than 3 µm particles
•5 µm Fused-Core particles can provide faster, more e�cient separations compared to 3 µm and 5 µm totally porous particles.
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Get Started
Additional resources are available for helping you implement Ascentis Express HPLC Columns into your laboratory.
WebVisit sigma-aldrich.com/express for videos, product information, ordering and real time availability information.
EmailOur technical service staff is ready to answer questions.
EU: eurtechserv@sial.com US: techservice@sial.com
In PersonA technical seminar can be arranged on-site or via the web. Request via seminars@sial.com.
I.D. Length C18 F5
2.1 mm 2 cm 50507-U 50603-U
2.1 mm 3 cm 50508-U 50604-U
2.1 mm 5 cm 50509-U 50605-U
2.1 mm 7.5 cm 50511-U 50607-U
2.1 mm 10 cm 50517-U 50612-U
2.1 mm 15 cm 50518-U 50613-U
2.1 mm 25 cm 50521-U 50614-U
3.0 mm 3 cm 50522-U 50615-U
3.0 mm 5 cm 50523-U 50616-U
3.0 mm 7.5 cm 50525-U 50619-U
3.0 mm 10 cm 50526-U 50622-U
3.0 mm 15 cm 50527-U 50623-U
3.0 mm 25 cm 50528-U 50624-U
4.6 mm 3 cm 50529-U 50625-U
4.6 mm 5 cm 50530-U 50626-U
4.6 mm 7.5 cm 50533-U 50627-U
4.6 mm 10 cm 50536-U 50628-U
4.6 mm 15 cm 50537-U 50631-U
4.6 mm 25 cm 50538-U 50632-U
Ordering Information
I.D. Length C18 F5
2.1 mm – 50539-U 50633-U
3.0 mm – 50541-U 50634-U
4.6 mm – 50542-U 50635-U
Ascentis Express 5 µm Columns Ascentis Express 5 µm Guard Cartridges, Pk of 3
Description Cat. No.
Holder w/EXP Titanium Hybrid Ferrule (cartridge not included)
53500-U
Ascentis Express Universal Guard Holder
Guard Cartridge
Holder
For more information about Ascentis Express, visit sigma-aldrich.com/express
Order/Customer Service (800) 325-3010 • Fax (800) 325-5052 Technical Service (800) 325-5832 • sigma-aldrich.com/techservice Development/Custom Manufacturing Inquiries (800) 244-1173 Safety-related Information sigma-aldrich.com/safetycenter
OSV11490-509521
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©2012 Sigma-Aldrich Co. LLC. All rights reserved. SAFC, SIGMA-ALDRICH and SUPELCO are trademarks of Sigma-Aldrich Co. LLC, registered in the US and other countries. Ascentis is a registered trademark of Sigma-Aldrich Co. LLC. Fused-Core is a registered trademark of Advanced Materials Technology, Inc. Supelco brand products are sold through Sigma-Aldrich, Inc. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see product information on the Sigma-Aldrich website at www.sigmaaldrich.com and/or on the reverse side of the invoice or packing slip.
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