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Collaborative possibilities with the Biomedical Research Foundation of the
Academy of Athensin the context of IMI
European Programmes Conference Week
6 December 2016
Nicosia, CyprusAristidis Charonis MD PhD
Biomedical Research Foundation of the Academy of Athens
About IMI
• Information about the Innovative Μedicines Ιnitiative can be found at www.imi.europa.eu
• It is the largest European initiative which is public-private and aims at speeding up the development of better and safer medicines for patients
• To achieve this goal it tries to built networks of industrial and academic partners in order to reach innovation in pharmaceutical products by supporting scientific excellence
A glance at the current budget (ΙΜΙ2)
• The current IMI programme has a total budget of about 3.300 million euros for 2014-2024
• The EU will contribute via the Horizon programme 1.638 million euros
• The EFPIA (European Federation of Pharmaceutical Industries and Associations) will contribute 1.425 million euros
• Other Associated Partners will contribute 213 million euros
The first period 2008-13 (IMI1)
• The budget for the first period was 2.000 million euros
• About 50 projects were funded
• Subjects varied, focusing on cancer, neurodegeneration, diabetes, osteoarthritis, bacterial resistance, vaccines, ect
Actions supported by IMI2
• Activities aiming to establish new knowledge
• Activities aiming to explore the feasibility of a new or improved technology, product, process or service
So, both basic and applied research proposals are accepted
How can Cyprus Institutes
collaborate with
the Biomedical Research Foundation
of the Academy of Athens
BIOMEDICAL RESEARCH FOUNDATION, ACADEMY OF ATHENS(BRFAA) - www.bioacademy.gr
MAIN MISSION OF BRFAA :
THE CATALYSIS OF TRANSLATIONAL RESEARCH
BIOMEDICAL APPLICATIONS
RESEARCH DIRECTIONS
CARDIOVASCULAR NEUROBIOLOGY
Development
Physiology - Pathology
Cellular Death
REGULATION OF GENE EXPRESSION
INFLAMMATION-TISSUE REMODELING
GENETICS OF AGEING
CANCER BIOLOGY
Basic Molecular Mechanisms
Gene Expression Networks
Genetic re-programming
Severe Asthma
Chronic Obstructive Pulmonary Disease
Interstitial Lung Diseases
Renal Failure
Rheumatoid Arthritis
Ulcerative Colitis, Crohn’s Disease
Mechanisms of carcinogenesis
Novel Markers
Cutting edge methodologies for:
Treatment and diagnosis
Gene Therapy
New drugs
Development
Neurodegenerative Diseases
Memory and Learning
Metabolic Diseases
Diabetes Obesity
Systemic Responses
In C. elegans
Telomeres
Systemic analysis
BIOLOGY OF STRESS
Animal Facility
Total Surface area: 1,600 sq.m.
Housing capacity: 20.000 transgenic mice, 1.000 conventional mice or
equal number of rats, 70 rabbits and 20 pigs.
ATLS COURSES, ENDOSCOPIC OTOLARYNGOLOGY. LIVER AND INTESTINE SURGERY,
ENDOSCOPIC SURGERY, ENDOSCOPIC ORTHOPEDICS, SPINAL CORD SURGERY,
PLASTIC SURGERY, ARTHROSCOPY, ROBOTIC SURGERY, LAPAROSCOPIC SURGERY
NUMBER OF COURSES: 119
NUMBER OF TRAINEES: 4.823
Surgery Rooms: Total surface area: 1,300 sq.m.
Four operating rooms, Two Intensive Care Units, Central Supply Area
Personnel involved:
A director, two Special Scientist, one head nurse, three technologists,
three animal specialists, two fully dedicated cleaning personnel
RESEARCH ACTIVITY 2004 - 2015
• Number of surgical procedures: 12.129
• Publications: 107
• Presentations in Congresses: 272
• Obtained awards: 15
BioImaging Unit (BIU)Two confocal microscopesOne is equipped for live cell imaging (with environment chamber, CO2 perfusion, very
fast live scanner). The other is on an upright fixed stage microscope, which makes it ideal for big samples such as whole animal embryo imaging
Fast Imaging system for quantitative intracellular measurements
Stereology and neuron tracing imaging workstation
Several automated microscopes with monochrome and colour high resolution digital cameras.
Image analysis suite with modern, wide screen computer workstations loaded with specialised Image Analysis software (Volocity, Imaris, LAS AF, Matlab, ImageJ etc).
All computers are linked via a fast (Gbit) network to two data servers (RAID5 - NAS servers) for more than 12TB safe, unified storage
Full time personnel involved
HEAD OF FACILITY: STAMATIS N. PAGAKIS, MS, PhD
MICROSCOPY SCIENTIST: ELENI RIGANA, MS, PhD
Clinical Imaging
• Imaging of live animals with high resolution ultrasound and positron emission tomography (PET/CT)
• Ultrasound and PET/CT technologies are also used for outpatient services
Dr. Dimitris Thanos (thanos@bioacademy.gr)
Dr. Alexandros Polyzos (apolyzos@bioacademy.gr)
FACS Analyzer Isolation of Cell
Subpopulations
Fluidigm C1Single Cell
Sequencing
Analysis
BD FACSAria™ III
Cell population sequencing
analysis
NextSeq 500
HiSeq 4000
MiSeq
NGS ExpressLibrary Preparation Robotic
System
Sequencing Platforms
single cell genomics
High Power
Computing Cluster
Mac Cluster
CyTOF2Single cell
proteomics analysis
PolarisSingle Cell
Functional Analysis
IrysStructural Variation
Detection
Analysis of Biological
Samples
NextSeq500
Study the transcriptome of 12 patients in 11 hours
Perform WGS for 1 patient in 29 hours (30x coverage)
Perform WES for 12 patients in 29 hours (100x coverage)
MAC cluster
CPU - 120 cores (threads)RAM - 0.5 TB
HARD DRIVES - 120 TB
Align > 400GB of data (WGS 30x) in less than 22 hours
Or Analyze the transcriptome of 24
patients in less than 24 hours
LINUX HPC
500 cores>2TB RAM
160TB HARD DRIVE for NGS analysis
>300TB HARD DRIVE for BACK-UP and STORAGE
Analyze (align) 16 Whole Genomes (30x) or > 6 TB of data
in less than a day
GGC techniques
4C-seqHiC
WGSWES
GGC projects
Germany
Freiburg – MAX PLANCK
Heidelberg - EMBL
Chania - MAICh Heraklion - IMBB
Ioannina - IMBB
Thessaloniki-AUTH
BRFAA Fleming NTUA UOA
New York - COLUMBIA
Seattle - UW
USA
• 50 Different Lab-projects
• 700 Sequenced Samples
• 70 Runs
• 2 publications in 1 year
PHARMACOLOGY UNIT WITH BIOANALYTICAL CAPABILITIES
DIVISION OF PHARMACOLOGY - PHARMACOTECHNOLOGY
The laboratory operates according to
the Good Laboratory Practice (GLP)
standards and has been awarded an
ISO/IEC 17025:2005 certificate.
The lab is equipped with Hybrid system of Liquid
Chromatography – Mass Spectroscopy of triple
quadrupole – Ion trap (LC-MS/MS), and all the
other equipment that are required to perform
bioanalysis and validation of drugs.
• Qualitative/ Quantitative analysis of drugs in biological fluids/tissues
• Pharmacokinetics (PK) of drugs in pre-clinical/clinical stages
• Drug metabolism
• Bioavailability and bioequivalence studies
• Quantitative determination of biomarkers (e.g. peptides, proteins) in various
matrices (e.g. plasma)
PROTEOMICS GENOMICS CYTOGENETICS
SUPPORT FACILITIES AT BRFAA
TransgenicsLaser Micro-dissection FACS-SORTING Lung Function
Measurements
Pan-Hellenic Research Infrastructure Networksthat will support Translational Research in Greece
(PIs: Drs Efsratiadis, Sideras, Thanos, Charonis, Kollias)
INFRAFRONTIER-GR
ΦΛΕΜΙΝΓΚIIBEAA
European Infrastructure for phenotyping and archiving of model mammalian genomes
BBMRI-GR
IIBEAAΦΛΕΜΙΝΓΚ, ΠΑΣΤΕΡ, EIE, ΕΚΠΑ, ΔΗΜΟΚΡΙΤΟΣ
ΠΙ
ΑΠΘΔΠΘ
ΠΘ
ΠΠ
ΠΚ, ΙΤΕΠΡ
Biobanking and Biomolecular Resources Research Infrastructure
EATRIS-GR
IIBEAAΦΛΕΜΙΝΓΚ, ΠΑΣΤΕΡ, EIE, ΕΚΠΑ
ΠΙ
ΑΠΘΔΠΘ
ΠΘ
ΠΠ
ΠΚ, ΙΤΕ
European Advanced Translational Research Infrastructure
Eager to collaborate…
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