conventional and molecular surveillance of paratuberculosis in tamil nadu

Conventional and molecular surveillance of paratuberculosis in

Tamil Nadu

Conventional and molecular surveillance of paratuberculosis in

Tamil Nadu

Vinodh Kumar,O.RDivision of Epidemiology

ICAR-Indian Veterinary Research InstituteIzatnagar, Uttar Pradesh, India

A1 , B1- negative controlC1, D1 – positive control

A1, A2- positive control

B1, B2 – negative control

Gamma interferon assay (Bovigam,Biocor,USA)

A1 , B1- negative controlC1, D1 – positive control

Out of 1034 sera samples tested 126 (12.24 % ) were positive to MAP IgG antibodies.

ELISA on 486 samples which represented Chennai and sub urban areas showed 78 were positive (16.04%).

Seropositivity in 460 sheep brought for slaughter at the local abattoir showed 44 (9.56%) were positive by ELISA

Districts Number of

samples

PCR Positives Percentage

Salem 220 54 24.55

Karur 37 4 10.81

Namakkal 35 6 17.14

Dharmapuri 41 5 12.20

Krishnagiri 28 4 14.29

Erode 82 15 18.29

Trichy 141 17 12.06

Perambalur 67 15 22.39

Ariyalur 25 7 28.00

Dindigul 72 8 11.11

Madurai 156 31 19.87

Thanjavur 37 4 10.81

Coimbatore 93 10 10.75

1034 180 17.41

Calvings Number

of

samples

ELISA Positivity

%

PCR Positivity

%

AFB Positivity

%

Heifer 32 14 43.75 21 65.63 17 53.13

First calving 87 19 21.84 24 27.59 4 4.60

Second

calving

527 57 10.82 92 17.46 38 7.21

Third calving 308 28 9.09 55 17.86 28 9.09

Fourth

calving

64 12 18.75 2 3.13 4 6.25

Fifth calving 16 9 56.25 6 37.50 0 0.00

Calvings vs percentage positivity

Districts Number of

samples

AFB positives Percentage

Salem 220 20 9.09

Karur 37 2 5.41

Namakkal 35 1 2.86

Dharmapuri 41 2 4.88

Krishnagiri 28 1 3.57

Erode 82 7 8.54

Trichy 141 12 8.51

Perambalur 67 5 7.46

Ariyalur 25 3 12.00

Dindigul 72 4 5.56

Madurai 156 12 7.69

Thanjavur 37 2 5.41

Coimbatore 93 8 8.60

1034 79 7.64

Districts Number of

samples

ELISA

positives

Percentage

Salem 220 40 18.18

Karur 37 2 5.41

Namakkal 35 3 8.57

Dharmapuri 41 3 7.32

Krishnagiri 28 2 7.14

Erode 82 8 9.76

Trichy 141 15 10.64

Perambalur 67 12 17.91

Ariyalur 25 2 8.00

Dindigul 72 7 9.72

Madurai 156 23 14.74

Thanjavur 37 2 5.41

Coimbatore 93 7 7.53

1034 126 12.19

Thank you