csii-ef-venus-nls...csii-ef-venus-nls 9959 bp mlui 228 agei 2668 apai 3187 agei 3760 xhoi 3766 ecori...
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CSII-EF-Venus-NLS
9959 bp
MluI 228
AgeI 2668
ApaI 3187
AgeI 3760XhoI 3766EcoRI 3771PstI 3776NotI 3781BamHI 3789
PstI 4002XhoI 4528
BamHI 4608HpaI 4624
ApaI 5941
CMV RU55' SD
RRE
3' SAcPPT+CTS
EF-1
VenusNLS
PREdel U3RU5BGH pA
f1 ori
SV40 pro/ori
Zeo
SV40 pA
ColE1 ori
Amp 1
Miyoshi
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Plasmid name: CSII-EF-Venus-NLS Plasmid size: 9959 bp Note CMV: Human cytomegalovirus immediate early promoter y: Packaging signal 5’SD: 5’ splicing donor site 3’SA: 3’ splicing acceptor site RRE: Rev responsive element cPPT: Central polypurine tract CTS: Central termination sequence EF-1: Human elongation factor 1 α subunit promoter Venus: A variant of yellow fluorescent protein (YFP) gene NLS: Nuclear localization signal PRE: Woodchuck hepatitis virus posttranscriptional regulatory element del U3: Deletion of enhancer and promoter sequences in the U3 region BGH pA: Bovine growth hormone polyadenylation signal SV40 pro & ori: SV40 early promoter and origin Zeo: Zeocin resistance gene SV40 pA: SV40 polyadenylation signal Amp: Ampicillin resistance gene
Miyoshi
テキストボックス
y
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