grds international conference on health and life (4)
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Enhancement of Lycopene and β-carotene Production in Cherry Tomato Fruits (Solanum lycopersicum L. var. cerasiforme) by Using Red and Blue Light Treatment
Calvin Lo, Robert Manurung, Rizkita Rachmi EsyantiSchool of Life Sciences and Technology
Institut Teknologi Bandung, Indonesia
calvinloe.92@gmail.com, manurung@sith.itb.ac.id, rizkita@sith.itb.ac.id
Introduction
• Lycopene prevention of cancer, cardiovascular disorder, etc.(Kong et al., 2010)
• β-carotene provitamin A & natural pigment.
• Prospective high economic value
(Pizarro & Stange, 2009)
(Kondo et al., 2014)
Aims of Study
• To determine the optimal light spectrum to enhancelycopene, β-carotene and sugar production in cherry tomatofruits.
• To construct mass distribution of lycopene, β-carotene andsugar production in plant treated with red, white and bluelight exposure.
Methods
Vegetative Growth
2
2.5
3
3.5
4
4.5
0 7 14 21 28 35
ln (
pla
nt
dry
we
igh
t)
Period of treatment (days)
Control
Red
White
Blue
Treatment Specific growth rate (day-1) Doubling time (days)Control 0.0572 12.2
Red 0.0642 10.8White 0.0719 9.6Blue 0.0677 10.2
Fruit Biomass Yield
0
100
200
300
400
500
600
700
Control Red White Blue
Tota
l mas
s o
f h
arve
ste
d f
ruit
(g
)
Treatment
Gilberto et al. (2005):Overexpression Cry2 inhibited flowering time
Sugar Content (Brix Analysis)
1 °Brix ≅ 10%-w of sucrose
0
2
4
6
8
10
Control Red White Blue
Suga
r co
nte
nt
(°B
rix)
Period of treatment (days)
Sugar 7 days
Sugar 28 days
Lycopene & β-carotene Content
0
50
100
150
200
Control Red White Blue
Lyco
pe
ne
& β
-car
ote
ne
co
nte
nt
(mg
/kg
fru
it)
Treatments
Lycopene 7 days
Lycopene 28 days
Beta-carotene 7 days
Beta-carotene 28 days
Mass Distribution
TreatmentVegetative biomass (g)
Fruit biomass (g)
Total amount produced in fruit
Lycopene (mg) β-carotene (mg) Sugar (g)
Control 41.33 47.7 63.78 17.46 41.25
Red 50.28 55.0 86.62 25.18 51.87
White 62.43 41.0 40.17 15.68 30.61
Blue 55.54 45.9 52.43 16.93 36.37
Conclusion
•Red light (663 nm) treatment enhanced lycopene, β-carotene andsugar production in cherry tomato fruits, with increment of 35.8%,46.5%, and 27.5% respectively.
•The best vegetative growth was observed on tomato plants treatedwith white light, while the highest yield of fruit (1.15 fold), sugar,lycopene and β-carotene were observed on plants treated with redlight.
Thank You
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