lab meeting
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Lab meeting. 2014.02.24 이세라. Gene Scan –B2M. PCR. electrophoresis. Sample 2. Sample 1. 95℃ / 5min. Sequencing-PLK1. Childhood AML -RNA. cDNA 합성. 65 ℃ / 5min -> ice. 2 5 ℃ / 10min -> 50 ℃ / 50min ->85 ℃ / 5min -> ice. 1 μ l of Rnase H. 37 ℃ / 20min -> ice. cDNA ->electrophoresis. - PowerPoint PPT Presentation
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Lab meeting
2014.02.24이세라
Gene Scan –B2MSample name 등록번호 Sample 날짜
양회식 70952242 11/09 CRS 환자
신원홍 70951849 11/16 CRS 환자
PCR
Sample 2.Sample 1.
electrophoresis
95℃ / 5min
Sequencing-PLK1
Childhood AML -RNA
cDNA합성
65 ℃ / 5min -> ice
25 ℃ / 10min -> 50 ℃ / 50min->85 ℃ / 5min -> ice
1μl of Rnase H 37 ℃ / 20min -> ice
cDNA->electrophoresis
cDNA PCR->electrophoresis
PLK1 -Primer
pyrosequencing
A-365B-2009-129D-4
D-22
2014.02.24(BDNF)
PCR전 PCR후2013.09.24(SLC6A4)
flowcytomety2014.02.21
G-MDSCM-MDSC
G-MDSCM-MDSC
2/20sample 조일도 71411924
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