mesa 2.4. myriam calle
Post on 12-Feb-2017
182 Views
Preview:
TRANSCRIPT
Mesa 2. Control y biomarcadores
Dra. Myriam
Calle Rubio
Hospital Clínico Universitario
San Carlos. Madrid
Mesa 2. Control y biomarcadores
[ATS] Inflammatory
Markers In Different COPD
Subgroups Compared To
Smokers
And Healthy Controls
Behndig AF
Mesa 2. Control y biomarcadores
Curtis et al: Proc Am Thorac Soc 2007; 4:512-521
Mesa 2. Control y biomarcadores
Inflammatory Markers in Different COPD Subgroups
Compared to Smokers and Healthy Controls
The chronic airway inflammatory response in COPD is suggested to be a result of both
innate and adaptive immune responses.
Natural killer cells (NK) and CD56-expressing T cells are cytotoxic lymphocytes that have
been implicated in COPD pathogenesis. Natural Killer cell group 2 D (NKG2D) receptors
have also been shown to be involved in emphysema development.Titular tabla
We hypothesised that cytotoxic T cells would be associated to the rate of lung function
decline in subjects with COPD. Two groups of COPD subjects were recruited, rapid
decliners (loss of FEV1 of > 60 ml/year) and slow decliners (loss of FEV1 of < 25 ml/year).
Am J RespirCrit Care Med 191;2015:A2884
Mesa 2. Control y biomarcadores
METHODS
As controls, we included two groups; smokers with normal lung function and healthy never
smoking individuals. All subjects were recruited from the OLIN-cohort (Obstructive Lung
disease In Northern Sweden) that has been followed with spirometry annually for a period
of more than 10 years.
Bronchoscopy with bronchoalveolar lavage was performed. T cell subsets were
determined in BAL-fluid from 17 individuals with COPD stage II-III (12 rapid decliner
and 5 slow decliners), and compared to data from 15 smokers with normal lung
function and 14 healthy never-smokers. The cells were stained with monoclonal
antibodies against CD3, CD8, CD16, CD56 and NKG2D, and analyzed using flow
cytometry.
Am J RespirCrit Care Med 191;2015:A2884
Mesa 2. Control y biomarcadores
RESULTS
In BAL-fluid NK-cells (CD3-/CD16+/CD56+) were higher in COPD subjects compared to healthy
(p=0.013). The expression of CD 16+/CD56+ on CD3+lymphocytes was increased both in
subjects with COPD with rapid decline in lung function (p<0.001), in COPD with slow decline
(p=0.0082) as well as in smokers with normal lung function (p=0.0065), all compared to healthy
non-smokers. Furthermore, the activating receptor NKG2D on CD8 positive lymphocytes was
found to be up-regulated in rapid decliners (p=0.0078) compared to healthy, but not in slow
decliners and in smokers.
CONCLUSIONS
NK-cells and CD56-expressing T cells were increased in COPD subjects regardless of rapid
or slow lung function decline. These data do not support the hypothesis that cytotoxic T cells can
be predictive for the rate of lung function decline in COPD. The increase in NKG2D in rapid
decliners may indicate that these cells can play a role in lung function decline. Further
research is warranted to confirm this finding.
Mesa 2. Control y biomarcadores
[SEPAR] Evaluación de
redes de interacción entre
biomarcadores sistémicos
de reactantes de fase aguda
en la EPOC
Calerio C
Mesa 2. Control y biomarcadores
EPOC e inflamación
Mesa 2. Control y biomarcadores
¿Sus implicaciones?
• Se desconocen sus mecanismos
de génesis.
• Se relaciona con comorbilidades,
algunos resultados clínicos y el
pronóstico.
Mesa 2. Control y biomarcadores
¿Papel de los marcadores de inflamación en el suero de los
pacientes con EPOC?Estudio Ciudad de Copenhague
N= 1302 EPOC
Seguimiento 8 años
Dahl et al; AJRCCM 2007; 175:250-255
Mesa 2. Control y biomarcadores
Subtítulo de la diapositiva
•Cabecera párrafo
Titular tabla
El objetivo era evaluar la relación entre diversos biomarcadores que actúan como
reactantes de fase aguda en la EPOC, para aproximarnos a conocer su impacto e
implicaciones.
Mesa 2. Control y biomarcadores
Estudio observacional, trasversal de pacientes con EPOC en fase
estable.
Determinación de 9 proteínas: alfa 2 macroglobulina, haptoglobina, PCR,
amiloide sérico P, procalcitonina, ferritina, activador tisular del
plasminógeno (tPA), fibrinógeno, amiloide sérico A.
Se estudiaron las correlaciones
bivariadas mediante un mapa de
calor.
Mesa 2. Control y biomarcadores
Existe un patrón de relaciones entre los biomarcadores reactantes de
fase aguda en la EPOC, que se modifica respecto a fumadores sanos y
distintos grados de afectación funcional.
Mesa 2. Control y biomarcadores
Muchas gracias
por su atención
top related