new uses of cord blood
Post on 18-Dec-2014
786 Views
Preview:
DESCRIPTION
TRANSCRIPT
Mahendra Rao- NIHCRM
New Uses of Cord Blood
The Cord Blood Industry
Hybrid • Korea
Other stem cells
MSC and Cord
Banking other cells
Making iPSC
HSC derivatives
• Public cord blood units are HLA typed • FACT offers accreditation services • US FDA has required licenses for public banks and we
expect this to be extended to private banks • The use of cord blood in children has exceeded the use of
bone marrow in children
Private eg: • Beike • China Cord
Blood • CBR
Public eg: • NYCBB • Carolinas • ClinImmune
• Private cord blood banks have proliferated and prices for storage have dropped.
• Most private large cord blood are in Asia and there is some consolidation and the average units stored in banks is in the range of 100K units
• Likely 50% of the units are too small for therapeutic use
Expanison of CBB’s
T cells, NK cells etc.
Potential other uses of cord blood
1: Expansion of cord blood 2: Identifying other stem cell populations a: MSC b: Hemangioblast c: Endothelial cells d: Differentiated blood products 3: Storing and using Placental tissue and cord 4:: Using infrastructure to store and process other cells 5: Making iPSC 6: Transdifferentiating to other cell types
Name/Website ViviCells
International, Inc. http://www.vivicells.
com
MEDIPOST http://www.medi-
post.com/
Lifeline Cord Blood Bank
Thai StemLife http://www.thaistemlife.co.
th
Vita 34 AG http://www.vita34.
de/
Cord Blood America http://www.cordblood-
america.com
Anthrogenesis Inc.
Eticur GmbH http://www.eticu
r.de
Cordlife Group Limited
http://www.cordlife.com
Meko Stem http://www.mekoste
m.com/
MAZE Cord Blood Laboratories
http://www.mazecordblood.com
Community Blood Services
Public Bank http://www.communitybloodserv
ices.org
The Institute For Transfusion Medicine Clinical Services,
ITxMCS, Cord Blood Services http://www.givcord.org/
StemCyte Inc. http://www.stemcy
teinc.com
Cord Blood Bank of Canada Inc
http://www.cordbloodbankofcanada.com
AssureImmune LLC
http://www.assureimmune.co
m/
FamilyCord http://www.famil
ycord.com/
CReATe Cord Blood Bank
http://www.createcordbank.com
MiracleCord, Inc http://www.miraclec
ord.com/
Karocell Tissue Engineering
http://www.karocell.com
NHS Cord Blood Bank
http://www.nhsbt.nhs.uk/
Insception http://www.insception.com
/
Smart Cells International
http://www.smartcells.com/
Cord Blood Registry http://www.cordblood.
com
BioE, Inc. http://www.bio
e.com
Future Health Biobank
http://www.futurehealth.co.uk
CRYOBANKS INTERNATIONAL,
INC. http://www.cryo-
intl.com National Stem Cell,
Inc. http://www.national
stemcell.com
Lazaron Biotechnologies (SA) Ltd
http://www.lazaron.co.za
StemLife Malaysia
http://www.stemlife.com
Celgene Corporation http://www.celgene.com
PacifiCord http://www.pacific
ord.com/
Cord Blood Solutions http://www.cordbloods
olutions.com/
Biohellenika http://www.biohe
llenika.ro
Healthbaby Biotech Co. Ltd
http://www.healthbaby.hk/
Cryo-Cell International, Inc. http://www.cryo-
cell.com
NeoCells http://www.neocells.
com/
Lifebank http://lifebank.com/
StemOne Biologicals Pvt.
Ltd. http://www.ste
mone.co.in
Cells For Life http://www.cellsforlife.com
/
Progenics Cord Blood Cryobank
http://www.progenicscryobank.com
Canadian Cord Blood Registry
http://www.ccbr.ca
BioLife Solutions Inc
http://www.biolifesolutions.co
m
Healthcord http://www.healt
hcord.com/
Cryo-Cell Mexico http://www.cryo-
cell.com.mx/
NeoStem, Inc. http://www.neostem
.com
LifebankUSA, Inc. http://www.lifebankusa.c
om
Stem Cell Institute
http://www.stemcell.co.jp/
China Cord Blood Corporation
http://www.chinacordbloodcorp.com
Relicord http://www.relicor
d.com/
Biosafe http://www.biosafe.ch
Bionet http://www.babybanks.com/2009/bionet-en
Héma-Québec http://www.hema-quebec.qc.ca
Cryocord http://www.cryocord
.com.my
Netcells Cryogenics http://www.netcells.
co.za/
LifeCord International http://english.lifecord.co.
kr
Stem Sciences, Inc
http://www.stemsciences.com/
CorCell, Inc. http://www.corcell.com
Saneron CCEL Therapeutics, Inc.
http://www.saneron-ccel.com
Alpha Cord http://www.alphacord.c
om/
CordBank http://www.cor
dbank.co.nz
HemaStem http://www.hem
astem.com/
Cryolife http://www.cryolife.
com.hk
New England Cord Blood Bank
http://www.cordbloodbank.com
Lifeforce Cryobanks http://www.lifeforcecryo
banks.com
ViaCord http://www.viac
ord.com/
Victoria Angel Registry of Hope Public Cord Blood
Bank http://www.cellsforlife.com
/victoriaangel/
ScienCell Research http://www.scienc
ellonline.com
Anthony Nolan Trust Cord Blood Bank
http://www.anthonynolan.org/
CordBancUSA
D-Finitive Cell Technologies
Cryo-Save http://www.cryo-
save.com
New Jersey Cord Blood Bank
LifeLine Cryogenics http://www.lifelinecryog
enics.com
SEWON Cellontech
http://www.cellontech.com
Virgin Health Bank http://www.virginhealthban
k.com/
Securacell http://www.secura
cell.com
Alberta Cord Blood Bank
http://www.acbb.ca/
Elie Katz Umbilical Cord Blood Program http://www.communitybloodservices.org/cb_
ekucbp.php
Cryosite http://cordblood.
cryosite.com/
Cord Blood Companies
ViviCells International, Inc. http://www.vivicells.com
MEDIPOST http://www.medi-post.com/
Lifeline Cord Blood Bank
Thai StemLife http://www.thaistemlife.co.th
Meko Stem http://www.mekostem.com/
MAZE Cord Blood Laboratories http://www.mazecordblood.com
Community Blood Services Public Bank
http://www.communitybloodservices.org
The Institute For Transfusion Medicine Clinical Services, ITxMCS, Cord Blood
Services http://www.givcord.org/
MiracleCord, Inc http://www.miraclecord.com/
Karocell Tissue Engineering http://www.karocell.com
NHS Cord Blood Bank http://www.nhsbt.nhs.uk/
Insception http://www.insception.com/
National Stem Cell, Inc. http://www.nationalstemcell.com
Lazaron Biotechnologies (SA) Ltd http://www.lazaron.co.za
StemLife Malaysia http://www.stemlife.com
Celgene Corporation http://www.celgene.com
NeoCells http://www.neocells.com/
Lifebank http://lifebank.com/
StemOne Biologicals Pvt. Ltd.
http://www.stemone.co.in
Cells For Life http://www.cellsforlife.com/
NeoStem, Inc. http://www.neostem.com
LifebankUSA, Inc. http://www.lifebankusa.com
Stem Cell Institute http://www.stemcell.co.jp/
China Cord Blood Corporation http://www.chinacordbloodcorp.com
Netcells Cryogenics http://www.netcells.co.za/
LifeCord International http://english.lifecord.co.kr
Stem Sciences, Inc http://www.stemsciences.c
om/
CorCell, Inc. http://www.corcell.com
New England Cord Blood Bank http://www.cordbloodbank.com
Lifeforce Cryobanks http://www.lifeforcecryobanks.com
ViaCord http://www.viacord.com/
Victoria Angel Registry of Hope Public Cord Blood Bank
http://www.cellsforlife.com/victoriaangel/
New Jersey Cord Blood Bank
LifeLine Cryogenics http://www.lifelinecryogenics.com
SEWON Cellontech http://www.cellontech.co
m
Virgin Health Bank http://www.virginhealthbank.com/
Vita 34 AG http://www.vita34.de/
Cord Blood America http://www.cordblood-america.com
Anthrogenesis Inc.
Eticur GmbH http://www.eticur.de
Cord Blood Companies
StemCyte Inc. http://www.stemcyteinc.com
Cord Blood Bank of Canada Inc http://www.cordbloodbankofca
nada.com
AssureImmune LLC http://www.assureimm
une.com/
FamilyCord http://www.familycord.com/
CReATe Cord Blood Bank http://www.createcordb
ank.com
Smart Cells International http://www.smartcells.com/
Cord Blood Registry http://www.cordblood.com
BioE, Inc. http://www.bioe.com
Future Health Biobank http://www.futurehealth.co.uk
CRYOBANKS INTERNATIONAL, INC.
http://www.cryo-intl.com
PacifiCord http://www.pacificord.com/
Cord Blood Solutions http://www.cordbloodsolution
s.com/
Biohellenika http://www.biohellenik
a.ro
Healthbaby Biotech Co. Ltd http://www.healthbaby.hk
Cryo-Cell International, Inc.
http://www.cryo-cell.com
Progenics Cord Blood Cryobank
http://www.progenicscryobank.com
Canadian Cord Blood Registry http://www.ccbr.ca
BioLife Solutions Inc http://www.biolifesoluti
ons.com
Healthcord http://www.healthcord.com
Cryo-Cell Mexico http://www.cryo-
cell.com.mx/
Relicord http://www.relicord.com/
Biosafe http://www.biosafe.ch
Bionet http://www.babybanks.
com/2009/bionet-en
Héma-Québec http://www.hema-
quebec.qc.ca
Cryocord http://www.cryocord.co
m.my Saneron CCEL Therapeutics,
Inc. http://www.saneron-
ccel.com
Alpha Cord http://www.alphacord.com/
CordBank http://www.cordbank.c
o.nz
HemaStem http://www.hemastem.com
Cryolife http://www.cryolife.co
m.hk
ScienCell Research http://www.sciencellonline.c
om
Anthony Nolan Trust Cord Blood Bank
http://www.anthonynolan.org/
CordBancUSA
D-Finitive Cell Technologies
Cryo-Save http://www.cryo-
save.com
Securacell http://www.securacell.com
Alberta Cord Blood Bank http://www.acbb.ca/
Elie Katz Umbilical Cord Blood Program
http://www.communitybloodservices.org/cb_e
kucbp.php
Cryosite http://cordblood.cryosite.com
Cord Blood Companies (con’t)
Blood collection and processing devices
Derivation of an iPSC
culture
Reprogramming
Candidate ESC like line
Select clones
Adult cells
Identify iPSC clones from partially programmed
Differentiated cell type
Grow propagate and bank
Around 1-5M
Multiple strategies with advantages and disadvantages
Around 21-28 days
1-6 rounds of infection Around 50-150 colonies
10-30 clones picked
Pick 3 to be safe per parent line
ESC like line
Test for differentiation
Test for pluripotency
Around 8 weeks-12 weeks
Around 5 weeks for EB stage
Around 6 weeks
Around 6 weeks
Why source from cord blood banks 1: Tissue sourcing is well organized and validated
2: HLA typing data is already being collected by banks
3: Existing samples can be used without compromising their ultimate use
4: A renumeration model already exists
5: Zerofootprint methods work well with cord blood cells
6: A Xenofree media and protocol has been developed so clinical grade iPSC lines can be made
7: Cord blood is the youngest source of stem cells one may obtain reliably and easily
8: Stem cells in general have specific mechanisms to maintain genomic integrity , delay senescence and protect against transformation.
9: The process can be easily extended to blood banks and marrow derived CD34 cells using the same infrastructure
10: Somatic memory and differentiation bias may work in our favor for early therapeutic efforts
11: May revive the cord blood banking business
Table 1: The relative advantages of making iPSC from either a private or a public cord blood bank are listed.
iPSC from blood or cord blood
•Fresh Harvest
•CD34+ cells or mononuclear fraction
•Confirm consent •Data Anonymization Issues •HLA typing data
•iPSC generation
•50,000 cells •Sendai •Electroporation •Vectors •IP issues •96 well format •Media and timeline
•At time of use of unit
•From designed bag or tubing •/ Non Ficoll process
•Cord Bank
•Receive at iPSC site
•Shipping •Tracking •Identity
•iPSC testing •Pluripotency •Differentiation •Karyotype
•Ship and store
Thank You Collaborators
top related