animal welfare training - 2013 animals in research & teaching biomedical models & basic...
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ANIMAL WELFARE TRAINING - 2013
ANIMALS in RESEARCH & TEACHING BIOMEDICAL MODELS & BASIC STUDIES
W.L. Shelton, Director
Laboratory Animal Resources
Institutional Animal Care and Use Committee
University of Oklahoma
http://iacuc.ou.edu
Time Line: 1966 - Public Law promulgation = Animal Welfare Act 1985 – USDA & NIH regulation revisions – established IACUC requirement
UNIVERSITY OF OKLAHOMA STRUCTURE of ANIMAL CARE
and USE PROGRAM*
LABORATORY ANIMAL RESOURCES (LAR)
INSTITUTIONAL ANIMAL CARE AND USE COMMITTEE (IACUC)
*Mandated by AWA of 1966 and amendments
Animal Care & Use Program
Implies the two major programmatic functions —
CARE = Animal husbandry = LAR
USE = Experimental Aspect = IACUC
STUDY and TEACHING CATEGORIES* Laboratory vs. Field – Manipulate vs. Observation Basic Studies (biology, behavior, husbandry)
Natural History Studies – native species in natural habitat Animal Models (genetics, physiology, medical, human surrogate) – Lab mammals, fish, amphib.
Biomedical –Lab Animals or Farm Animals or Native species Agricultural – Husbandry (Simulate farm environ.)
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*ALL LIVE VERTEBRATES COVERED BY ANIMAL WELFARE ACT
ETHICAL ISSUESHUMAN SUBJECT - Testing/Experimentation? - Institutional Review Board - Required
- Permission - Required- Placebos?- Drugs/Procedures?
ANIMALS – Cannot Obtain Permission!! Therefore: - Well being – Animal welfare - Appropriate animal model? - Information Value – Justification - Numbers – statistical validity/ minimum required
- Non-human Vertebrates – Sentience Consideration - Pain/ Stress alleviation – Required & Explained
Not everyone is enthusiastic about using animals in research
IS IT NECESSARY / IMPORTANT?
USE of ANIMALS in RESEARCH and TEACHING
Opponents vs Proponents
Animal Rights vs Animal Welfare
Animal Protection Societies were instrumental in the stimulation of legislation and subsequent development of the mechanism for
animal welfare protection relative to biomedical research
ANIMAL WELFARE PERSPECTIVE
Ecoterrorist and Protest groups have developed and operate outside the law
ANIMAL RIGHTS PERSPECTIVE
PETA opposes animal use – entertainment, food, experimentation.
Not above radicalization
ANTHROPOMORPHISM IS NOT SCIENTIFIC
STRUCTURE of ANIMAL CARE and USE PROGRAM*
LABORATORY ANIMAL RESOURCES
(LAR)
INSTITUTIONAL ANIMAL CARE and USE COMMITTEE
(IACUC)
*Mandated by AWA of 1966 and amendments
THE UNIVERSITY OF OKLAHOMA(A3240-01)
ANIMAL WELFARE ASSURANCEin accordance with the PHS Policy for
Humane Care and Use of Laboratory Animals
Update Approved 10 June 2013In effect through 30 June 2017
MANUAL of STANDARD OPERATING PROCEDURES (M-SOP)LABORATORY ANIMAL RESOURCES (LAR)
UNIVERSITY OF OKLAHOMA, NORMAN CAMPUS (OUNC)
February 2012 (3rd Edition)January 2009 (2nd Edition)
May 2005 (1st Edition)
William L. Shelton, LAR Director
Sutton Hall, 306405-325-1058
Administrative Office Sutton Hall, 308c
405-325-2609
Mailing address730 Van Vleet Oval Richards Hall, 314
University of OklahomaNorman, OK 73019
ANIMAL ORDER APPROVAL & CONFIRMATION FORM LABORATORY ANIMAL RESOURCES
Complete this form and send it to Director, Laboratory Animal Resource, Department of Biology, Richards Hall, Room 314.
Project Name & Animal Use Statement Number (R0X-XXX)
Project Number or Course Number: ____________________
Principal Investigator/ Instructor: ______ Phone #:_______
Housing of Animals: Building: _______ Room#: _______
Single : ____Group:______ Number Per Cage:____ NOTE: Animals will be segregated by sex unless otherwise directed.
Ordering Information:
Vendor: ______________________ Phone # Number:____
Name of Person Placing the Order: _______________
Species: ______ Sex: ________ Strain/Stock______________
Weight/Age: ________
Date Ordered: _________ Expected Deliver Date: _________
Deliver Animals to: ________________________________
Special Requirements:
Received in LAR office:________________ by:___________
Processed Form will be sent to the P.I./Instructor, the Animal Facilities Manager, and to the appropriate stockroom manager if ordered on P-card.
Update July 2013
University of Oklahoma, Norman Campus Animal Procurement, Use, and Disposal Procedures1
All Investigators must follow the appropriate procedures of the University of Oklahoma, Norman Campus LAR for procurement, use, and disposal of laboratory animals.
Ordering a. Complete the animal order form and email to LAR Director. b. Receive confirmation to proceed with the order; a processed copy of the order will be sent to the PI, animal facilities manager and to the appropriate Stockroom manager for P-card purchases. c. Call in order as described. d. Be available to receive the animals at delivery location where the animals will be housed.
Receiving a. The PI receives the animals and places in the appropriate cages which have been prepared by LAR personnel. b. Investigators will be responsible for the welfare of the animals until they are delivered to the housing facilities.
Housing a. Housing location will be determined by the Office of Lab Animal Resources, with the Investigator’s concurrence. b. Location of the research and well-being of the animals will be primary considerations. Disposal a. Biology PI’s & instructors will deliver carcasses and waste material to
the stockroom in Richards Hall, others will deliver this material to the Animal House. A special pickup each semester may be arranged for material from Comparative Anatomy.
b. Carcasses will be placed in plastic bags; no laboratory or surgical trash should be included.
c. Identify contents on a label, include investigator’s name, and whether contents have biohazards, infectious, or radioactive materials.
1 SOP-1 – Update Jul 2013
STRUCTURE of ANIMAL CARE and USE PROGRAM*
LABORATORY ANIMAL RESOURCES
(LAR)
INSTITUTIONAL ANIMAL CARE and USE COMMITTEE
(IACUC)
*Mandated by AWA of 1966 and amendments
IACUC
• Self-regulatory entity with responsibility to monitor animal research and the animal care program at institutions that use Live Vertebrate Animals IN RESEARCH or TEACHING
APHIS/USDA – Registered Research Facility OLAW/PHS - Institutional Assurance Compliance Document
= 4-year ‘contract’
Training• Federal law requires training for all who use
live animals in research & teaching
• Type of training depends on role– Supervisor and husbandry staff– Veterinarians – IACUC Members – Investigators
Signature of Major Professor -- Letter
Faculty and/or Graduate Student Research involving live
vertebrate animals--IACUC approval must precede research
--Documentation of IACUC review is
required by funding agencies & University:
Office of Research Support – proposals
Graduate College – thesis check-off
--Students must be named in approval letter if working on your Major Prof’s. AUS, OR You must have your own!
M.S. – COMMITTEE MEETING THESIS TOPIC FORM – TOO LATE!
DOCTORAL – ADVISORY CONFERENCE OK
IACUC Review Verification
for thesis/dissertation research
iacuc.ou.edu
Application procedure & forms
Links and Announcements
Training
IACUC Responsibilities*• Review Program – Semi-annually• Inspect Laboratory & Housing facilities – d.o.• Investigate suspected violations of AWA• Research Protocol Review - Monthly Personnel qualifications (Requisite training) Description of Literature Search Justification – protocol, species, numbers, & objectives Procedures to mitigate pain/distress Appropriate analgesia/anesthesia/euthanasia__________________________ *Report to Institutional Official
ELECTRONIC LITERATURE SEARCH
• Background or Foundational Studies
• Non-duplication
• Appropriate procedures
• Correct Animal Model
*Sentience? Pain?*(Conscious awareness of stimuli)
• Reactivity can & does occur without sentience• Many Complex Behaviors can be done without conscious awareness, including learned avoidance• Pain is sensed by cerebral cortex where it is perceived
and processed, but with no definite physical dimension.• However – USDA definition of Pain relative to AWA
Cerebral Cortex in CNS
Evolution of the Vertebrate CNS
Evolutionary Adaptation
CNS – Physiology - Behavior
RESEARCH & TEACHING
Biomedical
Ecological
Agricultural
iacuc.ou.edu
Thank You – Questions?