antioxidant role of mulberry (morus indica l. cv. anantha) leaves in streptozotocin-diabetic rats
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Antioxidant role of mulberry (Morus indica L. cv. Anantha) leaves in streptozotocin-diabetic rats. Bondada Andallu a *, N.Ch. Varadacharyulu b a Department of Home Science, Sri Sathya Sai Institute of Higher Learning, Anantapur-515001, India - PowerPoint PPT PresentationTRANSCRIPT
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Antioxidant role of mulberry (Morus indica L. cv. Anantha)leaves in streptozotocin-diabetic rats
• Bondada Andallua *, N.Ch. Varadacharyulub
• a Department of Home Science, Sri Sathya Sai Institute of Higher Learning, Anantapur-515001, India
• b Department of Biochemistry, Sri Krishnadevaraya University, India
系別:生科四甲
學號:91390391
姓名:吳佳煒Received 20 December 2002 received in revised form 7 July 2003; accepted 7 July 2003
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Abstract• The antihyperglycemic and
antioxidant role of mulberry (Morus indica L.) leaves were investigated.
Model : Wistar ratsI. Standard dietII. 25% dry mulberry leaf powder and
standard dietFor 8 weeks
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Abstract
antihyperglycemic and antioxidant role of mulberry effect :
• on blood glucose • lipid peroxidation• reduced glutathione (GSH) concentrations
and on the activity of glucose-6-phosphate dehydrogenase (G6PDH)
• various antioxidant enzymes in erythrocytes
♠Key point :
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Abstract
lipid peroxidation
fasting blood glucose concentrations glutathione GSH concentrations activity of glucose-6-phosphate
dehydrogenase (G6PDH) antioxidant enzymes
Results :
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Introduction
• Hyperglycemia 血糖過高症• Free radicals result in the consumption
of antioxidant defenses may lead to :a. disruption of cellular functionsb. oxidative damage to membranesc. enhance susceptibility to lipid peroxidation.
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Introduction• Total antioxidant status (TAS) of
serum is reduced in type I diabetes• The reactive oxygen species are
modulated by antioxidant enzymes :Glutathione reductase (GR) glutathione peroxidase (GPx)glutathione-S-transferase (GST)superoxide dismutase(SOD) catalase (CAT) non-enzymatic scavengers like reduced
glutathione.
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Introduction• Mulberry (M. alba L.) leaves containing many
nutritional components :• Flavones 黃酮• Steroids 類固醇• Triterpenes 三烯• amino acids 氨基酸• Vitamins 維生素• Moracin 桑辛素• other trace minerals 無機物
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Materials
• Male rats of Wistar strain from Hyderabad
weight range 150~200gair-conditioned room (25° ±1℃)light from 7 a.m. to 7 p.m.Divide into 4 groups
Animals
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Materials
I : normal control II : normal treated with
mulberry leaf powder III : diabetic controlIV : diabetic treated with
mulberry leaf powder
Wistar rats
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Materials
• The mulberry leaves (M. indica L. cv. Anantha)
• The 4th and the 5th leaves from the apex of healthy plants were plucked
• washed thoroughly under running tap water
• shade dried for 3 days and ground to a fine powder in an electric mixer
Leaves
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MethodsGroups III and IV (diabetic)by a single i.p. injection of streptozotocin
(STZ) (55 mg/kg) prepared in 1 ml of freshly prepared 0.1 mol/l citrate buffer after an overnight fast.
Groups I and II (Control) were injected with citrate buffer alone.
Streptozotocin injected animals were given 5% glucose water for 24 h following streptozotocin injection to prevent initial drug-induced hypoglycemic mortality
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Methods
•注射 streptozotocin ,以破壞分泌胰島素的β胰島細胞,進而形成高血糖的情形 。
•增加大鼠之進食量、飲水量及排泄量,並使體重變輕、血糖升高。
•人類糖尿病的症狀:吃多、喝多、尿多
streptozotocin
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Methods - Biochemical estimations
• Erythrocytes were separated from whole blood
• Erythrocytes were washed thrice with 0.9% NaCl
• suspended in 1 volume of 0.9% NaCl• The packed cell volume was adjusted
to 5% with PBS (pH 7.4)
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Student's T test
n
tsX
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Results & Discussion
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Fast
ing
Blo
od
Glu
cose
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Fast
ing
blo
od g
luco
se
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decreased food consumption insignificantly
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increased the body weight in diabetic rats
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• Mulberry decreased food consumption
insignificantly increased the body weight in
diabetic rats
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fagomine which potentiates the glucose induced insulin release similar to the action of glibenclamide a sulfonylurea drug which triggers the release of endogenous insulin
increase in tissue uptake of glucose by mulberry leaves
桑葉生物鹼 fagomine 可顯著增加葡萄糖引起的胰島素含量增加,從而降低糖尿病小鼠血糖。
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The effect of mulberry in controlling hyperglycemia and glycosuria could be due to:
the N-containing sugars isolated form mulberry leaves which inhibit the functions of
α-glucosidaseα-mannosidase β-galactosidase
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glucose-6-phosphate dehydrogenase 13%
glutathione reductase 34%glutathione peroxidase 20%glutathione-S-transferase 30%superoxide dismutase 46%Catalase 59%p < 0.01
diabetic control rats vs. normal controls
CATALASE 可氧化分解身體內過量的尿酸 . 將 H2O2 還原成 H2O 的反應 Glutathione peroxidase: 催化以 glutathione 為還原劑,將 H2O2 還原成 H2O 的反應
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• The activity of glucose-6-phosphate dehydrogenase (69%; p < 0.01)
• glutathione reductase (95%; p < 0.01), glutathione peroxidase (151%; p < 0.01)
• glutathione-S-transferase (24%; p < 0.01)• superoxide dismutase(106%; p < 0.01)• activity of Catalase 33% all p < 0.01
Diabetic control v.s mulberry
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Conclusion• Mulberry leaves are palatable and
effective in controlling hyperglycemia and glycosuria in STZ-diabetic rats.
• The leaves exerted a rapid protective effect against lipid peroxidation by scavenging O2 and elevating the activities of antioxidant enzymes by virtue of antioxidant flavonoids and moracins present in the leaves.
類黃酮 flavonoid 一類不含氮的生物色素,包括花色素 &FAA1; 和花黃色素。類黃酮在植物中存在普遍,但在動物中出現較少,具有抗癌的作用。
桑辛素 Moracin 這類化合物顯示了良好的抗菌和抗真菌作用,是植物抗真菌抗生素。
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Question & Answer
Thanks your listening