atnap, a nac family trancription factor, has an important role in leaf senescence
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学术报告 — 文献阅读汇报. AtNAP, a NAC family trancription factor, has an important role in leaf senescence. 报 告 人：王爽 专业年级：食品科学 2011 级 指导老师：寇晓虹副教授. Result and Conclusion. Introduction. Discussion. 1. 2. 3. 4. Outline. Experiment content. TF. - PowerPoint PPT PresentationTRANSCRIPT
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报 告 人：王爽专业年级：食品科学 2011 级指导老师：寇晓虹副教授
AtNAP, a NAC family trancription factor,has an important role in leaf senescence
学术报告—文献阅读汇报
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Outline
IntroductionIntroduction11
22
Result and ConclusionResult and Conclusion33
DiscussionDiscussion44
Experiment contentExperiment content
Introduction1.1 Leaf Senescence
Transcription FactorsTranscription Factors
TFTF 能够结合在某基因上游特异核苷酸序列上的蛋白质，活化后从胞质转位至胞核，通过识别和结合基因启动子区的顺式作用元件 , 启动和调控基因表达。
1.2 NAC family transcription factor
Introduction
WRKY
DREBbZIP
NACMYB
NAC
含有 NAC 结构域的蛋白统称为 NAC 蛋白
植物生长发育植物生长发育激素调节激素调节
环境胁迫应答环境胁迫应答
NAMNAM
CUC2CUC2
ATAF1/2ATAF1/2
Experiment content Inducible overexp-
ression of AtNAP
AtNAP expression in T-DNA line
Subcellular location of AtNAP
AtNAP expressionduring leaf senescence
3.1 AtNAP is upregulated during leaf sencence in Arabidopsis
Results and Conclusion
3.2 AtNAP is targeted to nuclei
Results and Conclusion
3.3 The AtNAP expression is knocked out in one T-DNA line and knocked down in another line
Results and Conclusion
3.4 Leaf senescence is significantly delayed in the atnap null mutant plants
Results and Conclusion
Results and Conclusion
Results and Conclusion
3.5 AtNAP homologs in rice and kidney bean are specifically expressed in senescing leaves
Results and Conclusion
Results and Conclusion
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3.6 AtNAP restores the atnap null mutant plants to wild type
Results and Conclusion
3.7 Inducible overexpression of AtNAP causes precious senescence
Results and Conclusion
Results and Conclusion
3.8 Microarray analysis of the expression profile of AtNAP
Results and Conclusion
RT-PCRRNA gel blot
T-DNA insertionsPlasmid constructionPlant transformationComplementation test
Disscussion
1.冶晓芳 ,唐益苗 ,高世庆 ,等 .植物NAC转录因子的研究进展 . 生物计数通报 ,2009,10(6)20–24.2.李鹏 ,黄耿青 ,李学宝 .植物NAC 转录因子 . 植物生理与分子生物学 ,2010,46(3)294–300.3.郭玉娟 ,魏强 .高羊茅中衰老相关转录因子AtNAP同源基因的克隆及功能分析 . 复旦学报 ,2010,49(5)544–558.4.彭辉 ,于兴旺 ,成慧颖 ,等 .植物NAC转录因子家族研究概况 . 植物学报 ,2011,45(2)236–248.报 ,2011,45(2)236–248.5.史红飞 ,高翔 ,陈其皎 , 等 .小麦NAC 转录因子的基因克隆与序列分析 . 西北农林科技大学学报 ,2011,31(3)395–401.6.易素华 ,张 丞 ,史志浩 ,等 .拟南芥花粉表面蛋白基因T-DNA插入突变体的鉴定 .上海师范大学学报 ,2010,39(2)313–318.7.尤扬 ,叶永忠 ,等 .植物激素对黄姜叶片Fv/Fo和Fv/Fm 的影响 .安徽农业科学 ,2007,35(19)5707–5703,5744.
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