SmartEnzymes™ Genovis AB

IgG Proteases

IgG Glycosidases

Site-specific

endopeptidase

Site-specific

Antibody

conjugation

O-Glycan

elimination

- 2 -

IgG Proteases FabRICATOR® (IdeS) p.3

FragIT™ p.3

FabRICATOR® (IdeZ) p.4

FabULOUS® (SpeB) p.5

GingisKHAN® (KGP) p.6

FabALACTICA™ p.7

IgG Glycosidases GlycINATOR® (EndoS2) p.8

Immobilized GlycINATOR® p.8

IgGZERO® (EndoS) p.9

deGlycIT™ (Immobilized IgGZERO®) p.9

Site-specific endopeptidase GingisREX™ p.10

Lysyl Endopeptidase® p.20

Site-specific Antibody conjugation GlyCLICK™

p.11-p.12

O-Glycan elimination

OglyZOR™ p.13

OpeRATOR™ p.14

SialEXO™ p.15

GlycOCATCH™ O p.16

Bio-pharma research products

KANEKA KanCapA™ p.17

Ni-NTA Ni- p.18

p.19

Lysyl Endopeptidase p.20

Line up & Index

No.

A0-FR1-020

FabRICATOR® (IdeS)

FabRICATOR® (IdeS) Enzyme 8 96

2,000 Units 94,300

A0-FR1-050 5,000 Units 182,900

A0-FR1-250 5 x 5,000 Units 734,900

A0-FR1-008 8 x 100 Units 64,400

A0-FR1-096 96 x 100 Units 379,500

A0-FR6-010

FragITTM – Immobilized FabRICATOR® FabRICATOR® (IdeS)

2 x 0.5 mg 70,200

A0-FR6-025 5 x 0.5 mg 159,900

A0-FR6-050 10 x 0.5 mg 264,500

A0-FR6-100 1 - 10 mg 212,800

A0-FR6-1000 10 - 100 mg 634,800

A2-FR2-005

FragITTM Kit FabRICATOR® (IdeS)

0.5 mg 78,200

A2-FR2-025 5 x 0.5 mg 212,800

A2-FR2-100 10 mg 265,700

A2-FR2-1000 100 mg 797,000

A0-FR8-020 FabRICATOR® LE (Low Endotoxin)

2,000 Units 103,500

A0-FR8-050 5,000 Units 201,300

A0-FR4-060 FabRICATOR® Validation Kit 3 x 2,000 Units 282,900

A3-AF1-010 Anti-FabRICATOR® 0.1 mL 55,200

FabRICATOR®

Native IgG

Human IgG1 ..CPAPELLG / GPSVF..

Human IgG2 ..CPAPPVA / GPSVF..

Human IgG3 ..CPAPELLG / GPSVF..

Human IgG4 ..CPAPELLG / GPSVF..

Mouse IgG1 X

Mouse IgG2a ..CPAPNLLG / GPSVF..

Mouse IgG2b X

Mouse IgG3 ..CPPGNILG / GPSVF..

Rat IgG2b ..CPVPELLG / GPSVF..

Rhesus Monkey ..CPAPELLG / GPSVF..

Rabbit ..CPPPELLG / GPSVF..

Species & Subclasses

X = no digestion confirmed

Hu

Hu

Hu

Hu

Mo

Mo

Mo

Mo

ICATOR eS)

FabRICATOR® (IdeS) His

FabRICATOR® (IdeS) 0.02 - 0.05% (w/v)

: 1 10mM 137mM NaCl 2.7mM KCl pH7.4 37 30 1μg IgG 95%

FabRICATOR® (IdeS) Streptococcus pyogenes E. coli

- 3 -

FabRICATOR®

Native IgG

Human IgG1 ..CPAPELLG / GPSVF..

Human IgG2 ..CPAPPVA / GPSVF..

Human IgG3 ..CPAPELLG / GPSVF..

Human IgG4 ..CPAPELLG / GPSVF..

Mouse IgG1 X

Mouse IgG2a ..CPAPNLLG / GPSVF..

Mouse IgG2b X

Mouse IgG3 ..CPPGNILG / GPSVF..

Rat IgG2b ..CPVPELLG / GPSVF..

Rhesus Monkey ..CPAPELLG / GPSVF..

Rabbit ..CPPPELLG / GPSVF..

Species & Subclasses

No.

A0-FRZ-020

FabRICATOR® Z (IdeZ)

FabRICATOR® Z Enzyme 2,000 Units 94,300

A0-FZ6-010

FragITTM Z – Immobilized FabRICATOR® Z FabRICATOR® (IdeZ)

2 x 0.5 mg 70,200

A0-FZ6-025 5 x 0.5 mg 159,900

A0-FZ6-050 10 x 0.5 mg 264,500

A2-FZ2-005 FragITTM Z Kit FabRICATOR® (IdeZ)

0.5 mg 78,200

A2-FZ2-025 5 x 0.5 mg 212,800

FabRICATOR® Z (IdeZ) His

FabRICATOR® Z (IdeZ) 0.02 - 0.05% (w/v)

: 1 10mM 137mM NaCl 2.7mM KCl pH7.4 37 2 1μg IgG 95%

FabRICATOR® Z (IdeZ) Streptococcus equi ssp. zooepidemicusand E. coli

1. pH FabRICATOR®

pH pH

(PBS) 6.0 – 8.0 HEPES 7.0 – 8.0

Tris 7.0 – 8.0 6.0 – 7.0

MES 5.5 – 6.5 6.0

FabRICATOR® pH5.0 pH8.0

- 4 -

No.

A0-PU1-020

FabULUOS® (SpeB)

FabULOUS® Enzyme 2,000 Units 94,300

A1-PFK-020

FabULOUS® Fab kit FabULOUS® (SpeB) Fab L -Kappa

2,000 Units 159,900

1.

A) IgG1

B) FabULOUS Fab kit

C) CaptureSelectTM LC-Kappa column

Fab

Waters Acquity

UPLC® BEH300 C4 RP-HPLC (Agilent

1290)

2. FabULOUS Fab kit IgG

Fab SDS-PAGE

1 IgG1

2 IgG1

3 CaptureSelectTM

4 Fab

5

FabULUOS® (SpeB) His

FabULUOS® (SpeB)

: 1 FabULOUS® 1μg IgG

FabULUOS® (SpeB) Streptococcus pyogenes E. coli

pH

(PBS) 6.5 – 8.0

Tris 7.0 – 8.0

6.5 *

1. pH FabULOUS®

(mM) pH (mM) pH

DTT 1-5 6.5 - 8.0 50-100 6.5 - 8.0

TCEP 1-5 6.5 - 8.0 2- 50-100 6.5 - 8.0

50-100 6.5 - 8.0

2. FabULOUS®

- 5 -

1. GingisKHAN FabKit Fab

(A) IgG (B) GingisKHAN

(C) CH1 CaptureSelect Fc

(D) Fab

Supelco C4 bioshell A4 RP-UHPLC (Agilent

1260)

2. GingisKHAN Fab kit IgG1 Fab

SDS-PAGE

1 6 2 IgG 3

GingisKHAN Fab Fc 4 Fc GingisKHAN

5 Fab

: 1 0.1M Tris pH8.0 37 1 1μg IgG1 95%

Porphyromonas gingivalis

No.

B0-GKH-020

GingisKHAN® (KGP)

GingisKHAN® Enzyme 2,000 Units 94,300

B0-GFK-020

GingisKHAN® Fab kit GingisKHAN® (KGP) Fab CH1

2,000 units 212,800

- 6 -

150mM NaP 0-150mM

37 pH6.5-8 IgG1 E:S 1:10 150mM NaP pH7.0 37

IgdE

SDS-PAGE Fig. 1c RP-HPLC Fig. 1d HPLC Waters Acquity UPLC®

BEH300 C4, 1.7 μm, 2.1x100 mm / 60

Agilent 1260

FabALACTICATM (IgdE) His

FabALACTICATM (IgdE)

: 1 150mM Sodium phosphate pH7.0 37 16-18 1μg IgG1 90%

FabALACTICATM Streptococcus agalactiae E. coli

No.

A0-AG1-020 FabALACTICATM 2,000 units 110,400

- 7 -

GlyclNATOR® (EndoS2) His

GlyclNATOR® (EndoS2)

: 1 10mM Sodium phosphate 150mM pH7.4 37 30 1μg IgG 95%

GlyclNATOR® (EndoS2) Streptococcus pyogenes in E. coli .

GlycINATOR® LE GlycINATOR®

(< 0.2 EU) 2,000 unit/vial

No.

A0-GL1-020

GlyclNATOR® (EndoS2)

GlycINATOR® (EndoS2) 2,000 Units 94,300

A0-GL6-010

Immobilized GlycINATOR® GlycINATOR®

2 x 0.5 mg 70,200

A0-GL6-025 5 x 0.5 mg 159,900

A0-GL6-050 10 x 0.5 mg 264,500

A0-GL6-100 1-10 mg 212,800

A0-GL6-1000 10-100 mg 634,800

A0-GL8-020 GlycINATOR® LE 2,000 Units 103,500

Immobilized GlycINATOR® MidiSpin

Deglycosylates 1-10 mg

Immobilized GlycINATOR®

Deglycosylates 0.5 mg Immobilized GlycINATOR® Maxispin

Deglycosylates 10-100 mg

- 8 -

IgGZERO® (EndoS) His

IgGZERO® (EndoS)

: 1 10mM Sodium phosphate 150mM pH7.4 37 30 1μg IgG 95%

IgGZERO® (EndoS) Streptococcus pyogenes E. coli

IgGZERO® LE IgGZERO®

(< 0.2 EU) 2,000 unit/vial

deGlycITTM MidiSpin

Deglycosylates 1-10 mg

deGlycITTM MicroSpin,

Deglycosylates 0.5 mg deGlycITTM Maxispin

Deglycosylates 10-100 mg

No.

A0-IZ1-010

IgGZERO® (EndoS)

IgGZERO® Enzyme 1,000 Units 47,200

A0-IZ1-050 5,000 Units 190,900

A0-IZ6-010

deGlycITTM – Immobilized IgGZERO®

IgGZERO® (EndoS)

2 x 0.5 mg 70,200

A0-IZ6-025 5 x 0.5 mg 159,900

A0-IZ6-050 10 x 0.5 mg 264,500

A0-IZ6-100 1-10 mg 212,800

A0-IZ6-1000 10-100 mg 634,800

A0-IZ8-020 IgGZERO® LE 2,000 units 103,500

IgGZERO® (EndoS) GlyclNATOR® (EndoS2)

TM MicroSpin

ates 0.5

EndoS)

ITTM MidiSpin

ates 1-10 mg

ITTM Maxispin

ylates 10-100

® (EndoS2)

- 9 -

GingisREX® (Rgp)

GingisREX® (Rgp) TCEP ( pH) Cysteine ( pH) DTT

GingisREX® (Rgp) Porphyromonas gingivalis

No.

B0-GRX-005 GingisREX® (Rgp) GingisREXTM – Arginine Specific Protease 5 μg 102,400

1. β GingisREX Arg-C 37

1:20 w/w 20mM pH7.4 GingisREX

pH7.6 Arg-C

RP-HPLC 1

1. 1. GingisREX Arg-C β

6 β

2. GingisREX Arg-C Herceptin®

GingisREX Arg-C 1:50 w/w

37

RP-HPLC

- 10 -

GlyCLICKTM conjugation of Alexa Fluor® 488

(L1-F01-025) to 250 μg IgG

GlyCLICKTM conjugation of Biotin

(L1-A01-025) to 250 μg IgG

GlyCLICKTM conjugation of DFO

(L1-C01-025) to 250 μg IgG

GlyCLICKTM conjugation of Azide Activation

(L1-AZ1-025) to 250 μg IgG

No.

L1-F01-025 GlyCLICK conjugation of Alexa Fluor® 488 to 250 μg IgG 1 kit 193,200

L1-C01-025 GlyCLICK™ DFO, labeling of up to 250 µg IgG 1 kit 193,200

L1-A01-025 GlyCLICK™ Biotin, labeling of up to 250 µg IgG 1 kit 193,200

L1-AZ1-025 GlyCLICK™ Azide Activation of 250 μg IgG 1 kit 172,500

- 11 -

Application

1. Waters Acquity UPLC® BEH300 C4, 1.7 μm, 2.1x100 mm /

65 Agilent 1290

Herceptin®

A. FabRICATOR® F(ab')2 Fc/2

B. Alexa Fluor®488 FabRICATOR® F(ab')2 Fc/2

C. B.

Application LC/MS

GlyCLICKTM conjugation of Alexa Fluor® 488

GlyCLICKTM conjugation of Biotin

GlyCLICKTM conjugation of DFO

GlyCLICKTM conjugation of Azide Activation

- 12 -

2. LC/MS FabRICATOR Waters BEH300 C4

Bruker Impact ESI Q-TOF Agilent 1290 UPLC

MaxEnt

A. Fc/2 LC

B. GlycINATOR®

C. Fc/2 GlyCLICKTM 245 Da DIBO MMAE 1,781 Da

2,026 Da

OglyZOR™ SialEXO™ His

OglyZOR™ SialEXO™

: 1 OglyZOR™ 1 SialEXO™ 20mM Tris pH6.8 37 2 1μg TNFαR O-

90%

OglyZOR™ Streptococcus oralis E. coli SialEXO™ Akkermansia muciniphila E. coli

No.

G2-OG1-020 OglyZOR™ OglyZOR™

(OglyZOR™ Enzyme + SialEXO™) 2,000 units 182,900

- 13 -

OglyZOR™

OglyZOR™ TNFR

SialEXO™ 1 37 OglyZOR™ 3 O-

1. OglyZOR SialEXO

O-

OpeRATOR™ SialEXO™ His

OpeRATOR™ SialEXO™

: 1 OpeRATOR™ 1 SialEXO™ 20mM Tris pH6.8 37 2 1μg TNFαR 90%

OpeRATOR™ Akkermansia muciniphila E. coli SialEXO™ Akkermansia muciniphila E. coli

No.

G2-OP1-020 OpeRATOR™ OpeRATOR™

(OpeRATOR™ Enzyme + SialEXO™) 2,000 units 159,900

OpeRATOR™

- 14 -

SialEXO™ His

SialEXO™

1 SialEXO™ 20mM Tris pH6.8 37 2 1μg Fetuin 90%

SialEXO™ Akkermansia muciniphila E. coli

No.

G1-SM1-020 SialEXO™ SialEXO™ 2,000 units 113,900

OglyZOR™ OpeRATOR™

- 15 -

SialEXO™ SialEXO™ α2-3 α2-6 α2-8

SialEXO™

1. 3’-sialyllactose α2-3 6’-

sialyllactose α2-6 Colominic

acid α2-8

SialEXO™

1. H2686 H4062 H8390 β

IOB Glycodrosocin

SialEXO™ GlycOCATCH™

O-GalNAcGal

Glycodrosocin

RP-LC C18 Advance BioPeptide

Map 2.1x100 2.7µm [Agilent ] ESI-Q-

TOF Bruker Impact II mass spectrometer

O-GalNAcGal

No.

G3-OC6-002 GlycOCATCH™ 200 μg 136,900

- 16 -

- 17 -

111-01081

KANEKA KanCapA™ Prepacked Column

1 mL 22,000

117-01083 5 mL 60,000

115-01084 5 5 mL 240,000

Bio-pharma research products

114-01071

KANEKA KanCapA™

2 mL (net 1 mL) 10,000

110-01073 10 mL (net 5 mL) 40,000

118-01074 50 mL (net 25 mL) 150,000

- 18 -

146-09731 Ni-NTA Cartridge

1 (5 mL) 13,000

142-09733 1 5 (5 mL) 55,000

147-09761

Ni-NTA Agarose

2 mL Net 1 mL 4,500

143-09763 10 mL Net 5 mL 10,500

141-09764 100 mL Net 50 mL 62,000

149-09684

Ni-NTA Agarose HP

2 mL Net 1 mL 85,000

145-09681 10 mL Net 5 mL 13,000

141-09683 100 mL Net 50 mL 100,000

081-10381 1 L 12,000

297-73101 BCA 250 tests 15,000

637-25715

500 mL 8,700

635-25711 500 mL 10 70,000

- 19 -

125-05061 20 μg×5 19,500

125-02543 1 vial (2AU) 8,800

129-02541 1 vial (10AU) 41,000

127-06621 1 (5AU) 25,000

295-50201 50 19,500

202-15951 20 μg×5 17,900

299-58901 20 tests 19,000

293-57701 20 tests 34,000

®

19101.5 01J

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