Bio-bandage which injects medicine to organ directlyusing extension of the Hydrogel

서강대학교 화공생명공학과 연성물질공학연구실 .김정민 김영대 양태경 임승모

서강대학교 화공생명공학과 - 화공생명공학심화설계 , 2014

Background

Manufacture process

Bandage for organ

Invivo bandage!

+ injection drugs to organ directly

+ dissolve itself in body Bilayer structure

Objective

Previous study

Experiment & Result

Step1. Prepare Hydrogel Step2. 1st Layered glass

Step5. Chain-polymerization of 2nd hydrogel

Curvature of bilayerHydrogel properties

technogel,, plastic solution company

Hydrogel Timoshenko equation

Extension / Micro size processing Physical property / Micro size

Step3. Photopolymeriztion of 1st Hyrogel

Step4. Stack 2nd layered glass

Drug is injected by adequate circumstance

l l

t1

t2

E1

E2

=

m= /

Network structure polymer

Properties of hydrogel are similar with the cellEasy processing

Drug release by specific Temperature, ionic/magnetic force, PH or wavelength

Application

Pilot test product

1st gel chain

2nd gel chain

Step6. Remove glasses & residue

Degassing Hydrogel

Degassing HydrogelResidue should be removed

Control degree of crosslinking

Control bilayer thickness

Low Modulus hydrogel (Extension occur)

High Modulus hydrogel(Forming hard structure)

Remove gas in molecules by vacuum pressure

Remove Oxygen

Bio-bandage Control of injected materials Where can we use Bio-bandage?

Control of injection timeAdvantage

Change types of medicine

Extension of hydrogel by liquids

Possible to inject drug of your choice

Possible to control the quantity of in-jected medicine

Control of duration of medicine efficiencyControling the quantity of crosslink can change duration of drug emission

Duration of medicine

- Damaged organ- Place of sutures- Bleeding wound

Attach Bio-bandage to damaged or wounded organ

After attached, medicine continuously is injected by extension of hydrogel

Hard structure that prohibit gel deformation or damaged

1st hydrogel

Similar properties with cell

2nd hydrogel

Inject medicine to organ by extension of hydrogel

Low extension that prohibit gel expansion to wrong area

Control of size or shape

Swelling

Swelling by ModulusControl of gel size easilyControl of shape by Timo-shenko equation

HydrogelUsually used bio-material

Easy control of releasing

Easy control of size

No technology for drug deliv-ery to organs

Drug delivery

Purpose Hydrogel bandage

Similar properties with organ

OctoDex, Octoplus companyCure surgical site

We can control releasing time and types of drug

Hydrogel has good bio-compability

We can find hydrogel is appropriate for drug delivery

So We attempt to develop bio-bandage for internal organs

(a) Schematic degradation process (b) photographs of hydrogels in dry state and during degradation process

We can make biodegradable hydrogel with natural polymers such as collagen, DNA Also, with degradable cross linking agent , we can synthesize biodegradable hydrogel

This hydrogel is liquid in room temperature. So it is more convenient to use and more applicable material to medi-cal field

We can make bio-adhesive hydrogel synthesizing pluronic-thiol ( Plu-SH)and catechol-functionalized hyaluronic acid.It is designed to mimic the properties of mussel

No needs to additional operation for removing bandage

Bio-adhesive

Transportation of drug

Swelling

Dried hydrogels have drugs - tight network structure

A

A

B

B

Experiment of sample’s diffusion Experiment of diffusion rate

0min 15min

30min

Being able to control sample diffusion rate us-ing cross link ratio

Bio-degradable

Expected occupation of demands

Swelled hydrogels release drugs- loose network structure

PolymerizationPhotopolymerization

Chainpolymerization

1. Drug can be transported directly without additional

injecting medication.2. We can control the site where drug is injected .

4. The method of uses is simple.

the hydrogel bandage can be applied for biotechnology such as surgical operation, plastic surgery, bio-sensor, drug delivery, etc.

Diffusion rate of sampleDifference of cross link ratio

Cross linker Monomer Water(dye) Initiator7 50 140.8 2.029 50 138.8 2.0211 50 136.8 2.0212 50 135.8 2.0213 50 134.8 2.0215 50 132.8 2.02

Crosslinker ratio

Diffused dyeDiffusion

Measure average intensity on rec-tangle of UV dye by ZEN software

Detection of UV dyeMeasure intensity of UV dye

Intensity is positively time dependent

3. We can control the effective duration of medicine