BIOmem

Glucose sensor

Une belle histoire…

- Concept -- Clark (1956)- Concept -- Clark (1956)

- Enzyme electrode -- Lyons and Clark (1962)- Enzyme electrode -- Lyons and Clark (1962)

HISTORY OF BIOSENSORS

•Oxygen sensor: [O2] current

Glucose Gluconic acid

Oxygen Hydrogen Peroxide O2 H2O2

Glucose Oxidase

- Concept -- Lyons and Clark (1956)- Concept -- Lyons and Clark (1956)

- Enzyme electrode -- Lyons and Clark (1962)- Enzyme electrode -- Lyons and Clark (1962)

- Glucose Analyser -- Yellow Springs Instr. Co. - Glucose Analyser -- Yellow Springs Instr. Co. (1973)(1973)

HISTORY OF BIOSENSORS

• Peroxide sensor: [H2O2] current

Glucose Gluconic acid

Oxygen Hydrogen Peroxide O2 H2O2

Glucose Oxidase

1975 : YSI 23A Blood Glucose AnalyzerFirst whole blood glucose analyzer (YSI, USA)

1982 : YSI 2300 STAT Plus Glucose & Lactate Analyzer

Glucose analyseur commercialisé par YELLOW SPRINGS Instr. Co.

- Concept -- Lyons and Clark (1956)- Concept -- Lyons and Clark (1956)

- Enzyme electrode -- Lyons and Clark (1962)- Enzyme electrode -- Lyons and Clark (1962)

- Glucose Analyser -- Yellow Springs Instr. Co. - Glucose Analyser -- Yellow Springs Instr. Co. (1973)(1973)

- Enzyme Electrode – MediSense Inc. (1987)- Enzyme Electrode – MediSense Inc. (1987)

HISTORY OF BIOSENSORS

• Mediator (ferrocene) e- current

Glucose Gluconic acid

Oxygen Hydrogen Peroxide O2 H2O2

Glucose Oxidase

1983 : Glucose capteur avec médiateur électrochimique

Glucose Gluconic acid

GOD-FAD GOD-FADH2

Ferricinium Ferrocene

1 e-

RFe

+R

Fe

THE MEDISENSE* EXACTECH™ GLUCOSE biocapteur pour le sang

*MediSense is now owned by AbbottSince 1996 (867 million $)

WalMart ReliOn™ (MediSense ™)

®

2005 : GlucoWatch G2 (Cygnus Inc., USA) : based on electroosmosis and electrochemical measurement of interstitial fluid

Evolution récente : système non invasif

GLUCOSE

SENSOR

Market opportunity

-Diabetus

Biology -Glucose oxidase

Instrumentation-portable

Electronics-data processing

Chemistry-polymers-electrode materials-mediators

Glucose sensors

OthersTHE BIOSENSOR MARKET

The growing global problem of diabetesStatistics 1995 2000 2025 (estimate)People with diabetes (million) 135 154 300% in developed countries 25.2 23.8 17.1Female/male ratio 1.18 1.16 1.12

Source: World Health Organisation.(Newman & Turner, 2005)