blueface presentation (kj)
TRANSCRIPT
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Conservation Genetics of the Blueface Darter (Etheostoma sp. cf. zonistium), a rare
undescribed fish in northwest Alabama
Kenny Jones Brook L. Fluker
Bernard R. Kuhajda
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Distribution and Characteristics
Bandfin Darter, Etheostoma zonistium
Blueface Darter, Etheostoma cf. zonistium
Fish art: Joseph R. Tomelleri
AL
MS
TN
KY
Gulf Coastal
Plain
Cumberland Plateau
Bailey and Etnier (1988); Kuhajda and Mayden (upubl. data)
Blueface Darter description is forthcoming (Kuhajda and Mayden).Once formally recognized, conservation status will need to be determined.
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Previous Work
Phylogenetic analysis of cytochrome b (mtDNA) data identified Blueface Darters as a monophyletic group
Sister to E. zonistium sp. group
Approximately 3% sequence divergence
Kuhajda and Fluker (2009)
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An Isolated Distribution
Natural isolation: The Hubbard Creek population is potentially isolated from other populations via natural isolation.
Reservoir-induced isolation: The Upper Bear Creek systems are possibly isolated due to the construction of the Upper Bear Cr. Reservoir (1978). Extant
Possibly extirpatedKuhajda and Mayden (2002)
Sipsey Fork
Little
Bear C
r.
Bear Cr.
Bear C
r.
Turke
y Cr.
Tennessee RiverDrainage
Black Warrior River Drainage (Mobile Basin)
Hubba
rd Cr.
Sipsey ForkUpper Bear
Cr. Reservoir
10.2 km
5.4 km
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QuestionsI. Is there significant genetic structure between
Blueface and Bandfin Darters?II. Does the Blueface Darter have reduced
genetic variation compared to the Bandfin?III. Is there significant genetic structure within
the Blueface Darter across the Tennessee/Black Warrior divide?
IV. Does the Black Warrior population of Blueface Darter have reduced genetic variation compared to the Tennessee River drainage populations?
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Methods: Sample collection
Upper BearCr.
Reservoir2
ALMS
TN
KY
Little
Bear C
r.
Bear Cr.
Bear
Cr.
Turke
y Cr.
Tennessee RiverDrainage
Black Warrior River Drainage (Mobile Basin)
Hubbard Cr.1
3
5
Sipsey Fork
Upper BearCr. Reservoir 2
6 478
9
Kinlock Falls, Hubbard Creek
20-30 individuals per site (seine) Fin-clipped (95% EtOH) Photo-vouchered or formalin preserved
specimens Extracted DNA using Qiagen DNeasy Kit
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Methods: Microsatellite DNA analyses Genotyped 187 individuals for 8
microsatellite DNA loci: Esc132b, Esc68, Esc18, Esc26b (Gabel
et al. 2008) Eca46, Eca48, Eca49 (Tonnis 2006) EosD107 (Switzer et al. 2008)
Estimated genetic structure using AMOVA (ARLEQUIN) and STRUCTURE
Estimated genetic variation via allelic diversity and heterozygosity expected (ARLEQUIN)
Evaluated differences in genetic variation using non-parametric statistics (Kruskal-Wallis and Mann-Whitney U tests)
Structure v2.3.4
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ResultsUpper Bear
Cr. Reservoir
2
ALMS
TN
KY
Little
Bear C
r.
Bear Cr.
Bear
Cr.
Turke
y Cr.
Tennessee RiverDrainage
Black Warrior River Drainage (Mobile Basin)
Hubbard Cr.1
3
4
Sipsey Fork
Upper BearCr. Reservoir 2
6 578
9
Results
1 63 4
Blueface Darter Bandfin Darter
2 5 7 8 9
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FCT= 0.02, and an overall P-value of 0.03
1 2 3 4 5 6 7 8 9
Hubbard Above --Hubbard Below 0.003* --Bear Cr. (cf. zon) 0.098 0.066 --Little Bear Cr. 0.146 0.109 0.060 --
Turkey Cr. 0.079 0.073 0.022* 0.109 --
Big Sandy 0.070 0.041 0.033 0.067 0.064 --
Birdsong 0.073 0.056 0.034 0.076 0.070 0.023 --
Hatchie 0.247 0.228 0.205 0.234 0.247 0.176 0.155 --Bear Cr. (E. zon) 0.168 0.140 0.119 0.162 0.158 0.090 0.104 0.246 --
Results cont.
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Blueface DartersBandfin Darters
Results
0
2
4
6
8
10
12
14
16
18
Blueface darters show a 23% reduction in genetic variation compared to Bandfin, but not significant (P = 0.084)
Mea
n Al
lele
s Per
Lo
cus
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ResultsUpper Bear
Cr. Reservoir
2
ALMS
TN
KY
Little
Bear C
r.
Bear Cr.
Bear
Cr.
Turke
y Cr.
Tennessee RiverDrainage
Black Warrior River Drainage (Mobile Basin)
Hubbard Cr.1
3
4
Sipsey Fork
Upper BearCr. Reservoir 2
6 578
9
Results
1 63 4
Blueface Darter Bandfin Darter
2 5 7 8 9
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1 2 3 4 5
Hubbard Above --
Hubbard Below 0.003* --
Bear Cr. (cf. zon) 0.098 0.066 --
Little Bear Cr. 0.146 0.109 0.060 --
Turkey Cr. 0.079 0.073 0.022* 0.109 --
Results cont.
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Mea
n Al
lele
s Pe
r Lo
cus
Genetic diversity differed between Blueface populations (P=0.018). Turkey Creek lower than all others.
Hubbard Above Hubbard Below Bear Cr. Little Bear Cr Turkey Cr.0
5
10
15
20
25
Results
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ConclusionsBlueface and Bandfin darters are differentiated with both
microsatellite and mtDNA. Species validity is supported with genetic data, although microsatellite data indicate retention of ancestral alleles in the Bear Creek population.
Overall, Blueface Darters do not have lower have lower genetic variation compared to the Bandfin Darter, even though allelic diversity was 23% percent lower and was a non-significant value.
Populations of the Blueface darter are isolated across the Tennessee/Black Warrior divide.
Possible reservoir fragmentation effect seen in Little Bear Creek, i.e. significant genetic structure and lower genetic variation compared to Bear Creek.
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Conservation implications After being formally
described, the results generated from this study can inform state and federal agencies about the conservation needs of the Blueface darter
Conservation units Hubbard Creek Bear Creek system
Conservation priorities1. Turkey Creek 2. Hubbard Creek3. Little Bear Creek4. Bear Creek (source for
PTRA)
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Acknowledgements
Funding Arkansas State University Faculty
Research Award Committee (FRAC). Tennessee Aquarium Conservation
Institute. Field and Lab Assistance
Alexandra Hook Taylor Lee Brittany McCall Dave Neely
Collecting Permits Alabama Dept. of Conservation and
Natural Resources Tennessee Wildlife Resources
Agency
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Closing Statement